Fig. 1

Expression of VaPYL4 is induced by cold stress. A Phylogenetic analysis of PYL ABA receptors from Vitis vinifera and Arabidopsis. The phylogenetic tree was constructed using MEGA5.0 by NJ method with bootstrap replicates of 1000. B Expression profiles of PYLs in V. amurensis in response to cold treatment. The expression data were collected from the previous RNA sequencing results (GSE166247). Data are means of three replicates ± SD. C Expression of VaPYL4 upon cold treatment. Two-month-old in vitro V. amurensis plants were treated at 4℃ for 48 h, and the leaves of cold-treated plants were collected at 0, 2, 4, 8, 12, 24 and 48 h during the cold treatment. The plants grown under normal conditions (25℃) were also sampled as the controls (CK) at each time point. The relative expression level of VaPYL4 was determined by quantitative real-time PCR (qPCR). Data are shown as means ± SD from three biological replicates. Significant differences were determined using Student’s t-test. **P < 0.01