Fig. 5

Analysis of overexpressed LaMYC4 gene in Arabidopsis plants. Wild type (WT), transformed by the empty vector pCAMBIA2300S (2300) and overexpressed LaMYC4 gene (35S::LaMYC4) plants (#2, #7). (a) Total contents. (b) monoterpenoids. (c) sesquiterpenoids. (d) Relative expression level of LaMYC4 as verified by qRT-PCR. (e) Relative expression level of At5g23960 as verified by qRT-PCR. (f-j) GC trace of caryophyllene. The number on the peak represents the peak area. The products were identified by comparison with compounds in the library NIST14 and reference standards. The values shown are mean ± SD at least three replicates. Standard errors are indicated as vertical lines on the top of each bar, and bars annotated with different letters were significantly different according to Fisher’s LSD test (P < 0.05) after ANOVA