Fig. 6

qRT-PCR analysis of miRNAs (A) and correlation of qRT-PCR (log₂ scale) and sequencing data of these miRNAs (B). FN39 and ROC22 were sugarcane mosaic disease-resistant and -susceptible varieties, respectively. Using miR159 as an internal reference gene. Each bar represents the mean values of three replicates ± standard error (n = 3). The correlation analysis is performed by scatter plot, and the y-axis and x-axis in Fig. 6B respectively show log₂ ratio of selected miRNAs expression in SrMV-infected samples versus virus-free samples via qRT-PCR and sRNA-seq