Table 1 Effect of pre-culture duration, colchicine concentration, and exposure time on leaf blades survival, shoot regeneration and octaploid induction of P. hopeiensis
From: In vitro octaploid induction of Populus hopeiensis with colchicine
Treatment | Pre-culture duration (d) | Colchicine concentration (μM) | Exposure time (d) | Survival rate (%)a | Shoot regen- eration rate (%)a | No. of shoots regeneratedb | No. of octaploidb | Octaploid induction rate (%)a |
|---|---|---|---|---|---|---|---|---|
1 | 7 | 50 | 2 | 76.67 ± 11.55 | 66.67 ± 5.77 | 150 | 0 | 0.00 ± 0.00 |
2 | 7 | 100 | 3 | 43.33 ± 15.28 | 43.33 ± 15.28 | 82 | 0 | 0.00 ± 0.00 |
3 | 7 | 150 | 4 | 30.00 ± 10.00 | 26.67 ± 5.77 | 54 | 1 | 1.59 ± 2.75 |
4 | 9 | 50 | 3 | 76.67 ± 15.28 | 66.67 ± 11.55 | 178 | 1 | 0.69 ± 1.20 |
5 | 9 | 100 | 4 | 46.67 ± 11.55 | 40.00 ± 10.00 | 93 | 8 | 8.61 ± 0.68 |
6 | 9 | 150 | 2 | 50.00 ± 10.00 | 46.67 ± 5.77 | 125 | 4 | 3.32 ± 1.81 |
7 | 11 | 50 | 4 | 63.33 ± 15.28 | 53.33 ± 5.77 | 138 | 0 | 0.00 ± 0.00 |
8 | 11 | 100 | 2 | 86.67 ± 5.77 | 76.67 ± 5.77 | 190 | 0 | 0.00 ± 0.00 |
9 | 11 | 150 | 3 | 56.67 ± 11.55 | 56.67 ± 11.55 | 86 | 0 | 0.00 ± 0.00 |
总计 | - | - | - | - | - | 1096 | 14 | - |