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Fig. 1 | BMC Plant Biology

Fig. 1

From: Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat–Thinopyrum intermedium and wheat–Thinopyrum ponticum substitution lines

Fig. 1

In situ hybridization patterns of the six alien substitution lines. a GISH patterns of ES-9 (a1) and ES-10 (a2): Th. ponticum genomic DNA (green) as probe and CS genomic DNA as a blocker; GISH patterns of ES-23 (a3), ES-24 (a4), ES-25 (a5), and ES-26 (a6): Th. intermedium genomic DNA (green) as probe and CS genomic DNA as a blocker. b FISH patterns of ES-9 (b1), ES-10 (b2), ES-23 (b3), ES-24 (b4), ES-25 (b5), and ES-26 (b6): Oligo-pSc119.2 (green) and Oligo-pTa535 (red) as probes. c Mc-GISH patterns of ES-9 (c1) and ES-10 (c2): Th. bessarabicum (J) genomic DNA (green) and tetraploid P. spicata (St) genomic DNA (red) as probes, CS genomic DNA as a blocker; ES-23 (c3), ES-24 (c4), ES-25 (c5), and ES-26 (c6): Th. bessarabicum (J) genomic DNA (green) and diploid P. spicata (St) genomic DNA (red) as probes, CS genomic DNA as a blocker. The arrows indicate the alien chromosomes of the six substitution lines

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