Skip to main content

Table 2 Extant (fresh) Phalaris species specimens (USDA - GRIN) used for ITS sequencing, DNA concentration (ng/μl) of extracted samples, their corresponding DNA quality readings from the spectrophotometer (optical densities [OD] at two ratios: OD 260/280 and OD 260/230), amplification success (+/−), GRIN accession numbers and the published National Center for Biotechnology Information (NCBI) GenBank accession numbers

From: Variability in ITS1 and ITS2 sequences of historic herbaria and extant (fresh) Phalaris species (Poaceae)

Phalaris Species

DNA Concentration (ng/ul)

OD 260/280

OD 260/230

Amplification Success

GRIN Accession Number

GenBank Accession Number

P. aquatica

60.7

1.97

2.14

+

PI 476287

MN811177.1

80.7

1.97

2.06

+

PI 476288

n/a

59.9

1.96

1.93

+

PI 303825

MN811173.1

P. arundinacea

52.2

1.95

2.13

+

PI 241065

n/a

28.5

1.84

1.47

+

PI 422030 (‘Ioreed’)

MN811174.1

P. canariensis

53.6

1.89

1.99

+

PI 578800

MN811183.1

57.8

1.97

1.79

+

PI 578798

MN811184.1

  1. n/a denotes specimens with no GenBank sequence publication
  2. + denotes a successful PCR amplification
  3. - denotes an unsuccessful PCR amplification