Skip to main content
Download PDF

Poplar aquaporin PIP1;1 promotes Arabidopsis growth and development

BMC Plant Biology volume 21, Article number: 253 (2021) Cite this article

Abstract

Background

Root hydraulic conductance is primarily determined by the conductance of living tissues to radial water flow. Plasma membrane intrinsic proteins (PIPs) in root cortical cells are important for plants to take up water and are believed to be directly involved in cell growth.

Results

In this study, we found that constitutive overexpression of the poplar root-specific gene PtoPIP1;1 in Arabidopsis accelerated bolting and flowering. At the early stage of the developmental process, PtoPIP1;1 OE Arabidopsis exhibited faster cell growth in both leaves and roots. The turgor pressure of plants was correspondingly increased in PtoPIP1;1 OE Arabidopsis, and the water status was changed. At the same time, the expression levels of flowering-related genes (CRY1, CRY2 and FCA) and hub genes in the regulatory networks underlying floral timing (FT and SOC1) were significantly upregulated in OE plants, while the floral repressor FLC gene was significantly downregulated.

Conclusions

Taken together, the results of our study indicate that constitutive overexpression of PtoPIP1;1 in Arabidopsis accelerates bolting and flowering through faster cell growth in both the leaf and root at an early stage of the developmental process. The autonomous pathway of flowering regulation may be executed by monitoring developmental age. The increase in turgor and changes in water status with PtoPIP1;1 overexpression play a role in promoting cell growth.

Peer Review reports

Background

Plant growth derives from the meristem, and meristem cells divide for several cycles before expansion to their final volume [1]. The rate of individual cell expansion is considered to be a function of turgor pressure, cell wall properties and cell hydraulic conductivity [2, 3]. Sustained cell growth requires sufficient water; thus, cellular water movement and homeostasis are tightly controlled. Cellular water movement is mainly mediated by aquaporins (AQPs), which facilitate passive exchange of water across membranes [4]. The plasma membrane (PM) intrinsic protein (PIP) subfamily is a member of the AQP family [5]. Since the hydrophobic Casparian strip of the endodermal cells in the root severely restricts the radial transport of water, PIPs are regarded as the first threshold of radial water absorption on PM, responsible for up to 30%—90% of the root water permeability in more than ten plant species [5,6,7].

Given the indispensability of water for plant growth and the importance of PIP in water permeability, PIP should have a role in plant root growth. Delayed lateral root emergence was found in Arabidopsis pip2;1 [8], as an example. Given the large members in the PIP family and their functional redundancy, some PIP mutations show no significant differences in growth morphology, such as Arabidopsis pip1;2–1, pip1;2–2, pip2;2–1 and pip2;2–2 [9, 10], which makes it hard to investigate their role in plant growth. Lower root total length, surface area, and root volume and fewer root tips were found in OsPIP2; 1 RNAi transgenic plants [11], and obvious morphological deformation and developmental retardation were observed in BnPIP1 antisense transgenic tobacco [12], which provides evidence that PIP is involved in plant growth and development. Recently, much work has been done to improve plant growth via the genetic manipulation of PIPs. Although not all attempts were successful, better growth performance was observed in some PIP-overexpressing (OE) plants. Listed below are a few examples: VvPIP2;4 N OE grape [13], TdPIP2;1 OE wheat [14], RsPIP2;1 OE Eucalyptus [15], AtPIP1b, TdPIP1;1 and TdPIP2;1 OE tobacco [16, 17], RcPIP2;1, RcPIP2;2, and AcPIP2 OE Arabidopsis [18, 19].

Turgor pressure is an essential driver of plant cell growth. Sustained cell growth requires sufficient water to keep the stable turgor pressure above the yield threshold, while turgor pressure provides the physical driving force against the cell wall and thus promotes cell division [20, 21] and cell expansion [22,23,24]. Early in 1965, the relationship between the steady-state elongation rate and turgor pressure was recognized [25]. Later, it was reported that cell division was greatly stimulated as turgor pressure increased to 1.0 bar, while cell expansion was stimulated as turgor pressure increased above 3.0 bar in isolated radish (Raphanus sativus L., var. Red Prince) cotyledons [20].

In an early study, we found that root pressure participated in refilling the embolized vessels of poplar [26]. In this study, we conducted an experiment to detect the expression of poplar PIP genes in roots, stems and leaves through qRT-PCR, and the results showed that PIP1;1 and PIP2;8 were relatively highly expressed in roots compared to stems and leaves, and the relative expression level of PIP1;1 in roots was the highest (Fig. 1a). It should be noted that in this experiment, PtPIP2,5 and PtPIP2;6 were not included because the sequence similarity was very high, 99%, and no suitable primers were found when we conducted this experiment. However, later, a colleague in our lab found primers to successfully separate the two PIPs and determined that PtPIP2;5 and PtPIP2;6 are the most highly expressed PIPs in poplar roots [27]. qRT-PCR in this paper was carried out using two-month-old cutting poplar plants growing in a greenhouse, while Jiang et al. used tissue culture plantlets [27]. Despite the different growth conditions of the plants, the qRT-PCR results in this paper are consistent with those of the previous paper. Originally, we intended to investigate the involvement of the predominantly expressed aquaporins in root pressure. Thus, we conducted constitutive overexpression (OE) of the Populus PIP aquaporin PtoPIP1;1 with PtoPIP2;8 in Arabidopsis and unexpectedly found that PtoPIP1;1 OE resulted in early flowering. To our knowledge, it has not been reported that AQP accelerates flowering. In addition to PtoPIP1;1, we also chose PtoPIP1;3 and PtoPIP2;3 for comparison. Our choice depends on different amino acid residues in loopE of PtoPIP1;1, PtoPIP1;3 and PtoPIP2;3. The different amino acid residues in loopE are related to their different interactions with PIP2, resulting in different water permeability coefficients of PIP2 [28]. Accordingly, the different protein sequences in loop E of PtoPIP1;1, PtoPIP1;3 and PtoPIP2;3 (Figure S6) may lead to different interactions with other PIPs and hence different water permeability coefficients.

Fig. 1
figure 1

PIP gene expression in poplar tissues and subcellular localization of PtoPIP1;1. a The relative expression level of PIP genes in different tissues. EIF-5A was used as the internal control. Bars represent the standard error of the mean of three biological replicates. Means ± standard errors (n = 3) are shown. b Subcellular localization of PtoPIP1;1 in PIP1;1-YFP transgenic Arabidopsis under isosmotic conditions and hypotonic conditions. Bars = 50 µm

Full size image

Flowering is a typical event that occurs during the plant transition from vegetative growth to reproductive development, and five genetically defined pathways have been identified as controlling flowering: the vernalization pathway (VRN1, VRN3, etc.), the photoperiod pathway (CRY1, CRY2, etc.), the gibberellin pathway (GAI, RGA, SPY, etc.), the autonomous pathway (FCA, FPA, FVE, etc.) and the aging pathway [29]. LFY, FT, SOC1 and CO, however, are integrators in the regulatory networks underlying floral timing [29]. In this study, overexpression and wild-type plants grew in the same environment, so light or temperature could not be the cause of variation in flowering time. The autonomous pathway may monitor developmental age; plants must pass through a juvenile phase and reach the adult vegetative phase before they can flower [30]. Therefore, we hypothesize that the growth changes caused by aquaporins promote flowering in terms of aquaporin involvement in plant growth. Thus, we studied the growth rate and osmotic potential of transgenic plants to explore the mechanism by which AQP accelerates flowering. In a previous study, the effects of aquaporins on root hydraulic conductivity, growth, and even turgor pressure were inversely proven by inhibiting the activity of aquaporins with mercury [22, 31, 32]. In this study, we wanted to further explore the involvement of aquaporins in water conductivity, turgor pressure and growth by overexpressing aquaporins, as well as determining their relationship with flowering time.

Results

PtoPIP1;1-overexpressing Arabidopsis showed accelerated flowering

Fifteen PIP genes were identified in the genome of P. trichocarpa (Figure S1 and Table S1). The expression of poplar PIP genes in roots, stems and leaves was analyzed through qRT-PCR, and the results showed that PIP1;1, PIP2;7 and PIP2;8 were relatively highly expressed in roots among roots, stems and leaves, while the relative expression level of PIP1;1 in roots was the highest (Fig. 1a). Then, PIP1;1 was cloned from P. tomentosa, and its subcellular localization was investigated. Through transiently expressing the PtoPIP1;1-YFP fusion protein in leaf epidermal cells of N. benthamiana, the fluorescence signal of PtoPIP1;1-YFP merged well with FM4-64 and the endoplasmic reticulum (ER) marker HDEL, indicating that PtoPIP1;1 localized on the plasma membrane (PM) and ER (Figure S2). Consistent with the results, the localization of PtoPIP1;1 on PM was also observed in PtoPIP1;1-YFP transgenic Arabidopsis (Fig. 1b). Interestingly, under hypotonic conditions (water treatment), most PtoPIP1;1-YFP fluorescence signals were distributed at both ends of root epidermal and cortical cells (Fig. 1b), showing a polar-like localization pattern at the PM. This localization pattern of PtoPIP1;1 may reflect its dynamic response to different water conditions in facilitating water transport.

Compared to wild-type (WT), PtoPIP1;1 OE Arabidopsis showed an early-flowering phenotype regardless of whether it was grown under long-day (LD) or short-day conditions (SD) (Fig. 2). In addition, the OE PtoPIP1;1 lines had the same leaf number before bolting compared with nontransgenic control plants; however, the leaf development of the former was faster than that of the latter (Figure S3). In the developmental process (at 14 DAS), the expression levels of CRY1, CRY2 and FCA (genes in photoperiod and autonomous pathways) as well as FT and SOC1 (major hubs in the regulatory networks underlying floral timing) were significantly upregulated in OE plants, while the floral repressor gene FLC was significantly downregulated in OE plants (Fig. 3). Thus, overexpression of PtoPIP1;1 was able to accelerate Arabidopsis development.

Fig. 2
figure 2

Early flowering of PtoPIP1;1-overexpressing (OE) Arabidopsis. a Photographs of wild-type (WT) and PtoPIP1;1 OE Arabidopsis grown under long day (LD) and short day (SD) conditions. b Days of bolting of WT and two PtoPIP1;1 OE Arabidopsis lines (OE-1 and OE-5) under LD and SD conditions. Means ± standard errors (n = 20 or more) are shown. Data were analyzed statistically using Student’s t-test. **P < 0.01

Full size image
Fig. 3
figure 3

Expression of PtoPIP1;1 and flowering-related genes in WT and two PtoPIP1;1 OE Arabidopsis lines (OE-1 and OE-5). a The expression of PtoPIP1;1. b-e The expression levels of four flowering time pathway-related genes. f The expression levels of major hub genes in the regulatory networks underlying floral timing. The 14-DAS plants after 8 h of light (n = 3) were measured, and Actin2 was used as the internal control. Data were analyzed statistically using Student’s t-test. *P < 0.05. **P < 0.01. PIP (plasma membrane intrinsic protein); VRN (vernalization response); CRY (cryptochrome); GAI (gibberellin-insensitive); RGA (repressor of GA1-3); SPY (spindly); FCA (an RNA-binding protein); FPA (an RNA-binding protein); FVE (a WD-40 repeat protein); AP1 (apetala1); FT (flowering locus t); FD (flowering locus d); SOC (suppressor of overexpression of constans 1); LFY (leafy); CO (constans); FLC (flowering locus c)

Full size image

Leaf cell growth

To determine the mechanism of accelerated cell growth, the number and size of cells during leaf development were kinematically analyzed. The 3rd leaf was harvested every 2 days during the development process of WT and PtoPIP1;1 OE Arabidopsis; more precisely, the sampling time was from 11 to 19 days after stratification (DAS). The leaf size and palisade cell area were measured. The leaf area of PtoPIP1;1 OE plants was 45.3% and 17.5% larger than that of WT at 11 and 13 DAS, respectively, but comparable at 19 DAS (Fig. 4b). The palisade cell size of OE plants, however, was smaller than that of WT at 11, 13, and 15 DAS but similar at 17 and 19 DAS (Fig. 4a, c). The calculated cell number in OE plants was greater than that in WT at 11 and 13 DAS but nearly the same at 17 and 19 DAS (Fig. 4d). The higher cell division rate and larger leaf area of PtoPIP1;1 OE Arabidopsis than of WT at the early developmental stage (Fig. 4e, f) indicate that PtoPIP1;1 can promote leaf growth and development by accelerating cell division. Furthermore, the status of cell division of WT and PtoPIP1;1 OE Arabidopsis was analyzed at 11 DAS by flow cytometry. The results showed that the 4C fraction in PtoPIP1;1 OE Arabidopsis (55.6%) was much greater than that in WT Arabidopsis (31.5%) (Fig. 4g), which further indicates that PtoPIP1;1 could promote leaf cell growth.

Fig. 4
figure 4

Characterization of the 3rd leaf in WT and two PtoPIP1;1 OE Arabidopsis lines (OE-1 and OE-5). a Palisade cells of the 3rd leaf from WT and PtoPIP1;1 OE at 11, 15, and 19 days after stratification (DAS). Bar = 20 µm. b Leaf area, Means ± standard errors (n = 6 ~ 8) are shown. c Palisade cell area. The cell area was measured using 40 to 60 leaf cells from photographs of 6 to 8 leaves. d The calculated cell number of the 3rd leaf from WT and two PtoPIP1;1 OE lines. Means ± standard errors (n = 6 ~ 8) are shown. e The relative cell growth rate was calculated based on the data from A. The relative cell growth rate is expressed as the increase in cell area (µm2) relative to the previous cell area per unit of time (day). f The average cell division rate was calculated based on the data from A. The average cell division rate of 11-DAS seedlings is expressed as the average cell number per unit of time. g The ratio of 2C/4C cells from the 3rd leaf of WT and PtoPIP1;1 OE lines at 11 DAS by flow cytometric analysis of their nuclear DNA content. Data for the ratio of 2C/4C represent the mean of six biological replicates with multiple leaves pooled in each replicate. Data were analyzed statistically using Student’s t-test. **P < 0.01

Full size image

Root cell growth

Arabidopsis roots are also a good model to study cell division, elongation and terminal differentiation [33]; thus, root length, root meristem size, and root cell size were investigated in both WT and PtoPIP1;1 OE Arabidopsis. The root length of PtoPIP1;1 OE Arabidopsis was not significantly different from that of WT (Figure S4A, B); however, the root meristem size of PtoPIP1;1 OE Arabidopsis was significantly decreased by 24.5% (Fig. 5), indicating that root cell elongation-differentiation was promoted in PtoPIP1;1 OE Arabidopsis. In addition, the cell length of the 6th cell from elongating roots in PtoPIP1;1 OE Arabidopsis was 28.9% smaller than that of WT, whereas the length of elongated cells was not significantly different between WT and PtoPIP1;1 OE Arabidopsis (Figure S4C), which is consistent with the growth process of leaf palisade cells. The root length and root meristem size of another 2 PIPs, PtoPIP1;3 and PtoPIP2;3, OE Arabidopsis showed no difference from that of WT (Figure S5), indicating that not all of the PIPs were involved in the regulation of root cell growth.

Fig. 5
figure 5

Root meristem zone in WT and two PtoPIP1;1 OE Arabidopsis lines (OE-1 and OE-5). a Root meristem zone. Green and white arrowheads indicate the QC and the cortex TZ, respectively. The insert shows an enlarged view of elongating cells exiting from the meristem at the TZ. Bar = 10 µm. b Measurements of root meristem size represented in A. The root meristem size was measured with at least 20 roots in three independent experiments. Data were analyzed statistically using Student’s t-test. **P < 0.01

Full size image

Water loss and turgor pressure

Since water movement across the cell membrane is facilitated by AQPs [34], the difference in water loss between WT and PtoPIP1;1 OE Arabidopsis was examined. The water loss in PtoPIP1;1 OE Arabidopsis was significantly faster than that in WT (Fig. 6a), indicating that overexpression of PtoPIP1;1 changed the water status. Since turgor pressure plays pivotal roles in cell division and expansion/elongation [19, 24, 35], the osmotic potential of PtoPIP1;1 OE Arabidopsis was measured, and turgor pressure was calculated accordingly. Compared with WT Arabidopsis, PtoPIP1;1 OE Arabidopsis exhibited significantly lower osmotic potential and higher turgor pressure (Fig. 6b, c). Notably, the turgor pressure was increased by a value of approximately 0.05 osmol kg−1 (Fig. 6b), which is enough to promote cell division according to a previous study of radish cotyledons [19].

Fig. 6
figure 6

Physiological parameters detected in WT and two PtoPIP1;1 OE Arabidopsis lines (OE-1 and OE-5). a Water loss detected in WT and PtoPIP1;1 OE lines. Bars represent the standard error of the mean of at least 4 plants. b, c Turgor pressure (b) and osmotic potential (c) of WT and PtoPIP1;1 OE lines at 11 DAS. Data are shown as the means ± SE from six biological replicates. Data were analyzed statistically using Student’s t-test. **P < 0.01

Full size image

Discussion

In this study, we report that constitutive overexpression of PtoPIP1;1 in Arabidopsis accelerates plant development with rapid leaf and root cell growth. Turgor pressure was altered in PtoPIP1;1 OE Arabidopsis to a level that can affect cell growth.

The autonomous pathway for controlling flowering refers to endogenous regulators that are independent of the photoperiod, temperature and gibberellin pathways. All genes in the autonomous pathway act by repressing FLC expression [29], as shown in this study, in which FLC expression was significantly downregulated in OE plants. FCA (its gene was upregulated in this study) was shown to be associated with the downregulation of FLC [36]. On the other hand, the upregulation of FT and SOC1 also confirmed the downregulation of FLC [37]. In addition, cryptochromes (CRY) act to promote photomorphogenic development and the transition from vegetative to floral development [38]. The growth rate and growth amount may input signals to the flowering regulation pathway. As reported, Agalinis strictifolia flowered earlier than slower growing individuals [39], and larger plants flowered earlier and produced more flowers than smaller plants [40]. In this study, the earlier flowering Arabidopsis grew faster in both the root and leaf during the developmental process.

Through phenotypic and histocytological analysis, we found that PtoPIP1;1 OE Arabidopsis exhibited the same accelerated growth as the previously mentioned PIP OE plants [13, 14, 16, 18, 41, 42]. However, it should be noted that not all PIP OE plants showed improved growth. For example, AtPIP1;4 and AtPIP2;5 OE Arabidopsis and tobacco [43], MusaPIP1;2 and MusaPIP2;6 OE banana [44, 45], BnPIP1 OE tobacco [12], CsPIP1;1, CfPIP2;1, MfPIP2-7, ThPIP2;5, and MsPIP2;2 OE Arabidopsis [43, 46,47,48] were indistinguishable from their nontransgenic controls. In addition, the growth of some PIP OE plants was even inhibited, such as BjPIP1 OE tobacco [49], RsPIP1;1 OE Eucalyptus [15], and AtPIP2;1 OE Arabidopsis [8]. In our study, although PtoPIP1;1 OE Arabidopsis exhibited accelerated plant growth, the growth and development of PtoPIP1;3 and PtoPIP2;3 OE Arabidopsis were indistinguishable from those of WT. Therefore, not all PIP members can improve plant growth.

Cell growth occurs through cell division and cell expansion/elongation-differentiation [1]. In our study, the growth process of leaf palisade cells and root cells in PtoPIP1;1 OE Arabidopsis was similar. Leaf size was larger and palisade cell size was smaller in PtoPIP1;1 OE Arabidopsis than in WT at the early stage of leaf development, while leaf size and palisade cell size became similar to WT at the later stage of leaf development, indicating that cell division was promoted at the early stage of leaf development and that cell expansion was promoted at the late stage of leaf development by the overexpression of PtoPIP1;1. For roots, although root meristem size was decreased in PtoPIP1;1 OE Arabidopsis, the whole root length and the mature root cell size were similar between WT and PtoPIP1;1 OE lines, indicating that root cell elongation-differentiation was enhanced by the overexpression of PtoPIP1;1. In summary, the overexpression of PtoPIP1;1 accelerated leaf and root cell growth and thus leaf area growth at the early stage of the developmental process.

Turgor pressure is an essential driver of plant cell growth, including cell division, cell expansion and cell wall extension [19, 24, 35, 50]. In this study, the turgor pressure of PtoPIP1;1 OE Arabidopsis was approximately 1.3 bar higher than that of WT. It was reported that cell division could be greatly stimulated as turgor pressure increased to 1.0 bar, while cell expansion was stimulated as turgor pressure increased above 3.0 bar in isolated radish cotyledons [20]. Accordingly, the increased turgor pressure of PtoPIP1;1 OE Arabidopsis is enough to promote cell division. Although the underlying mechanism of turgor change in terms of PtoPIP1;1 expression is not clear, some plant aquaporins can transport small neutral solutes, such as glycerol, urea, formamide, acetamide, methylammonium, boric acid, silicic acid, lactic acid and CO2 (see review by Maurel et al. [5]), which can all affect the osmolality and turgor of cells. For example, aquaporin-mediated hydraulic conductivity reduction in maize roots by arid load and hydrogen peroxide and anoxia application was reported to have an impact on turgor pressure and leaf elongation rate [22], and CpPIP2 was suggested as a potential gene to adjust the turgor pressure-driven elongation of developing fibers in transgenic cotton [51]. In fact, the osmotic potential was decreased and the turgor pressure was increased in PtoPIP1;1 OE Arabidopsis, although the turgor pressure here was calculated but not directly determined. However, it should be noted that the turgor pressure estimation method used here was approved in comparison to the direct turgor pressure determination method using the cell-pressure probe, and the result showed good agreement between these two methods [52]. Interestingly, with the same material, PtPIP1;1 was found to participate in the osmotic stress response [27].

In this study, PtoPIP1;1 OE Arabidopsis exhibited accelerated plant growth, while the growth of PtoPIP1;3 OE Arabidopsis was indistinguishable from that of WT. The water loss experiment reflected changes in the water status and water hydraulics of PtoPIP1;1. Thus, overexpression of PtoPIP1;1 could increase the rate of passive water transport into roots, since all plants were grown on the medium in the plate with 100% relative humidity; under this condition, there was a considerable water potential gradient outside and inside the root. In addition, it was reported that WT plants may always suffer a water deficit due to suboptimal symplastic water transport via aquaporins even under favorable growth conditions [16]. Here, the accelerated plant growth observed in PtoPIP1;1 OE Arabidopsis further confirmed the increased water permeability coefficient caused by PtoPIP1;1. In addition, under hypotonic conditions, most PtoPIP1;1 proteins accumulated in both ends of root epidermal and cortical cells. This localization pattern of PtoPIP1;1 may reflect its dynamic response to different water conditions in facilitating proper water transport. This finding is consistent with a previous report showing that the hydraulic conductivity of a growing root cell should be seen more in the context of facilitating water flow through a tissue rather than facilitating water uptake at a sufficiently high rate into an individual cell to support that cell’s expansion growth [53].

Conclusions

Constitutive overexpression of the poplar root-specific gene PtoPIP1;1 in Arabidopsis accelerates flowering through faster cell growth in both the leaf and root at an early stage of the developmental process. The results of this study indicate that expressed PtoPIP1;1 can increase turgor pressure and that turgor pressure alteration promotes accelerated plant growth, at least at some stage of plant development. Accompanying accelerated growth, the genes involved in flowering regulation in the autonomous pathway changed. The autonomous pathway of flowering regulation may be executed by monitoring developmental age, and the expression levels of flowering-related genes (CRY1, CRY2 and FCA) and hub genes in the regulatory networks underlying floral timing (FT and SOC1) are significantly upregulated in OE plants. It is likely that the promotion of leaf growth and development is the first message, and the hub regulatory networks of genes in the autonomous pathway constitute the second message.

Methods

Plant materials and growth conditions

Arabidopsis thaliana ecotype Col-0, obtained from the Arabidopsis Biological Resource Center (ABRC) through TAIR (www.arabidopsis.org), was grown in a greenhouse at 21 °C and 16-h light/8-h dark with a relative humidity of 70% and a light intensity of 110 μmol m−2 s−1. Sterilized seeds were plated on 1/2 MS supplemented with 1% sucrose (Suc) and 0.8% agar. Seven-DAS (days after stratification) seedlings were transferred to soil and further grown for approximately two months to harvest seeds. For meristem size analysis and water potential measurement, transgenic seedlings were grown together with their corresponding WT plants on the same plate placed vertically.

The seedlings of a hybrid poplar crossing Populus alba and P. glandulosa (clone 84 K) were propagated by stem cuttings. Sections of stems 8 cm long with two or three lateral buds were rooted and grown for 2 months in plastic pots (30 cm deep × 26 cm diameter, one seedling per pot) filled with artificial soils in a half-controlled greenhouse at 20 °C—28 °C with relative humidity and photosynthetically active radiation fluctuating between 30 and 55%, 600 and 1200 μmol m-2 s-1, respectively. Pots were randomly rotated twice a week to minimize edge effects.

Subcellular localization

To determine the subcellular localization of PtoPIP1;1, attB sites were added via PCR-mediated ligation to the coding regions of PtoPIP1;1 without a stop codon and inserted into pDONR 222 according to the manufacturer’s protocol (Invitrogen). The cDNA was then transferred via LR reaction (Invitrogen) into the destination vector pEarleyGate 101(C-YFP), resulting in the 35S::PtoPIP1;1-YFP construct. Transient expression of PtoPIP1;1-YFP and GFP-HDEL in Nicotiana benthamiana leaf lower epidermal cells was performed using Agrobacterium transformation according to a previous report [54].

Plasmid construction and gene expression analysis

Populus PIP (Table S1) sequences were obtained from the Poplar Genome Database (http://www.phytozome.net/poplar.php, release 3.0) and NCBI database (http://www.ncbi.nlm.nih.gov/), while Arabidopsis PIP protein sequences were obtained from TAIR (http://www.arabidopsis.org/). Then, amino acid sequence alignment was performed using the CLUSTALX program (http://www.clustal.org/). A putative full-length expressed sequence (locus name Potri.010G191900) homologous to other PIP1s was obtained (Figure S1). The CDSs of PIP1;1, PIP1;3 and PIP2;3 were cloned from Populus tomentosa using the primers listed in Table S2. For overexpression, the purified PCR products were first inserted into the pMD-19 vector (TaKaRa) and confirmed by DNA sequencing. Then, XbaI and SalI were used to digest and ligate the sequences of PtoPIP1;1, PtoPIP1;3 and PtoPIP2;3 into the pBI121 binary vector using T4 ligase (NEB), resulting in the 35S::PtoPIP1;1, 35S::PtoPIP1;3 and 35S::PtoPIP2;3 constructs, respectively.

qRT-PCR in this paper was carried out using two-month-old cutting poplar plants (Populus alba × Populus glandulosa, clone 84 K) growing in a greenhouse. The third leaves from the apex were sampled for total RNA extraction, the xylem part from the middle portion of the stem was used for stem RNA extraction, and fine roots were used for root RNA extraction. Quantitative real-time PCR (qRT-PCR) analysis was carried out with a 7500 Real-Time PCR System (Applied Biosystems, CA, USA) using a SYBR Premix Ex TaqTM Kit (TaKaRa, Tokyo, Japan). Primers of Arabidopsis flowering-related genes [29] and PIP genes found in P. trichocarpa [26] for qRT-PCR analysis were designed with a length of 20–24 nucleotides and a predicted melting temperature of 59–61 °C (Table S3). PCRs were carried out in 20 µL final volumes containing 0.2 µM primers, 2 µL of cDNA and 10 µL of SYBR Premix Ex Taq™ (2 ×). The PCR program was 1 cycle of 30 s at 95 °C, followed by 40 cycles of 95 °C for 5 s and 60 °C for 34 s, with a final dissociation stage of 15 s at 95 °C, followed by 60 °C for 1 min and 95 °C for 15 s to verify the specificity of the primer pairs. PCRs without templates were used as negative controls. The final threshold cycle (Ct) values were the means of twelve values from three biological replicates with four technical replicates in each biological replicate. The relative transcript abundance was calculated using 7500 Real-Time PCR analysis software with EIF-5A for poplar [26] and Actin2 for Arabidopsis [55] as an internal control.

Plant transformation

35S::PtPIP1;1, 35S::PtPIP1;3, 35S::PtPIP2;3, and 35S::PtPIP1;1-YFP constructs were introduced into the Agrobacterium tumefaciens GV3101 strain. Agrobacterium-mediated transformation was performed using the floral dipping method [56] in Col-0 Arabidopsis thaliana. Putative transgenic plants were screened on 1/2 MS plates supplemented with 30 mg L−1 kanamycin and then transferred to soil for propagation. Kanamycin-resistant plants of the T2 generation were subjected to expression analyses. Homozygous T3 transgenic plants, together with WT plants, were used for further studies. In this study, a total of more than 10 overexpressing lines of PtPIP1;1 were generated, and all of them had an early flowering phenotype compared to nontransgenic control plants. Among them, two independent lines, OE-1 and OE-5, were selected for further functional analyses given the heavy workload of the experiment.

For fluorescence signal detection, roots from 35S::PtPIP1;1-YFP Arabidopsis were observed either in 1/2 MS or in water (hypotonic conditions) using a confocal microscope (Zeiss LSM 510). Images (Fig. 1b) shown are representative of at least three independent experiments with at least 10 roots in each solution.

Leaf growth analysis

Leaf growth was analyzed on the 3rd leaf harvested at different time points (11, 15 and 19 DAS). Leaves were fixed in formalin-acetic acid-alcohol (FAA) and then cleared in chloral hydrate solution (200 g of chloral hydrate, 20 g of glycerol, 50 ml of H2O). Leaves were then mounted in 100% lactic acid on microscopy slides. All observations were performed using a Zeiss LSM 510 AX70 with a differential interference contrast objective. The leaf size and cell area were measured using photographs of 6 to 8 leaves and 40 to 60 leaf cells with IMAGEJ software (http://rsb.info.nih.gov/ij/), and cell numbers were calculated accordingly. The cell area was measured in the region located 25–75% from the distance between the tip and the base of the blade, halfway between the midrib and the leaf margin. For the kinematic analysis, ln-transformed means of leaf area, cell area, and cell number were locally fitted to a quadratic function, of which the first derivative was taken as the relative growth and expansion, respectively [57, 58].

Flow cytometric analysis

Flow cytometric analysis was carried out according to a method reported previously [59]. The 3rd leaves were dissected from Arabidopsis seedlings at 11 DAS and chopped with a razorblade in 400 µL of nuclear extraction buffer. After the addition of 600 µL of PI (propidium iodide) (50 μg/mL) staining buffer, the supernatant was collected after filtering with a 30-µm filter. The distribution of nuclear DNA content was analyzed with a FACSCalibur flow cytometer (BD Biosciences, CA, USA). For each sample, 10,000 events were analyzed. The cell cycle was analyzed using ModFit LT 3.0 software. Data for the ratio of 2C/4C represent the mean of six biological replicates with multiple leaves pooled in each replicate. The nuclear 2C/4C ratio reflects the status of the cells within the cell cycle [60].

Root length and root-meristem size analysis

Roots were stained in a 10 µM PI solution (Sigma-Aldrich) for 2 min. The root meristem was defined as the zone of cortex cells extending from the quiescent center (QC) to the first elongated cell of the transition zone (TZ) [61]. Then, root meristem size was measured with an LSM 510 confocal microscope (Zeiss). Root length was measured using IMAGE J software (http://rsb.info.nih.gov/ij). Data on root length and root meristem size represent the means from three independent experiments with at least 20 roots.

Water loss speed assay

To quantify water loss, at least four 14-DAS seedlings were acclimated in the dark for 3 h prior to measurement. Then, each whole seedling was weighed immediately and weighed every 20 min using a microbalance (the relative humidity was between 40 and 50% and the temperature was approximately 22 °C). The proportion of water loss was calculated on the basis of the initial fresh weight of the samples.

Osmotic potential measurement and turgor pressure calculation

The 11-DAS seedlings were frozen in liquid nitrogen and then centrifuged for 20 min at 1,500 g in microcentrifuge tubes. Further separation of the cellular fluid from plant debris was obtained by centrifugation at 9,600 g for 10 min, and osmotic potential was measured using 30 µL samples at freezing-point depression with an Osmomat 030 (Gonotec, Berlin, Germany). Since the WT and PtoPIP1;1 OE plants all grew in plates with 100% relative humidity, the water potential of the plants was used as the value of the osmotic media. Based on the above osmotic potential and water potential, the turgor pressure was calculated and could be regarded as an estimated value of turgor pressure in plants [62]. PtoPIP1;1 OE Arabidopsis plants were grown together with their corresponding WT on the same plate. Osmotic potential was measured by using six biological replicates with multiple leaves pooled in each replicate.

Availability of data and materials

All data generated or analyzed during this study are included in this article and available from the corresponding author on reasonable request.

References

  1. Rymen B, Sugimoto K. Tuning growth to the environmental demands. Curr Opin Plant Biol. 2012;15:683–90.

    Article  PubMed  Google Scholar 

  2. Boyer JS. Water transport. Annu Rev Plant Physiol. 1985;36:473–516.

    Article  Google Scholar 

  3. Cosgrove DJ. Water uptake by growing cells: an assessment of the controlling roles of wall relaxation, solute uptake, and hydraulic conductance. Int J Plant Sci. 1993;154:10–21.

    Article  CAS  PubMed  Google Scholar 

  4. Chrispeels MJ, Agre P. Aquaporins: water channel proteins of plant and animal cells. Trends Biochem Sci. 1994;19:421–5.

    Article  CAS  PubMed  Google Scholar 

  5. Maurel C, Verdoucq L, Luu DT, Santoni V. Plant aquaporins: membrane channels with multiple integrated functions. Annu Rev Plant Biol. 2008;59:595–624.

    Article  CAS  PubMed  Google Scholar 

  6. Wan X, Zwiazek JJ. Mercuric chloride effects on root water transport in aspen seedlings. Plant Physiol. 1999;121:939–46.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Javot H, Maurel C. The role of aquaporins in root water uptake. Ann Bot. 2002;90:301–13.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Peret B, Li G, Zhao J, Band LR, Voss U, Postaire O, Luu D-T, Da Ines O, Casimiro I, Lucas M, Wells DM, Lazzerini L, Nacry P, King JR, Jensen OE, Schäffner AR, Maurel C, Bennett MJ. Auxin regulates aquaporin function to facilitate lateral root emergence. Nat Cell Biol. 2012;14:991–8.

    Article  CAS  PubMed  Google Scholar 

  9. Javot H, Lauvergeat V, Santoni V, Martin-Laurent F, Guclu J, Vinh J, Heyes J, Franck KI, Schäffner AR, Bouchez D, Maurel C. Role of a single aquaporin isoform in root water uptake. Plant Cell. 2003;15:509–22.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Postaire O, Tournaire-Roux C, Grondin A, Boursiac Y, Morillon R, Schaffner AR, Maurel C. A PIP1 aquaporin contributes to hydrostatic pressure-induced water transport in both the root and rosette of Arabidopsis. Plant Physiol. 2010;152:1418–30.

    Article  CAS  PubMed  Google Scholar 

  11. Ding L, Uehlein N, Kaldenhoff R, Guo S, Zhu Y, Kai L. Aquaporin PIP2;1 affects water transport and root growth in rice (Oryza sativa L.). Plant Physiol Bioch. 2019;139:152–60.

    Article  CAS  Google Scholar 

  12. Yu Q, Hu Y, Li J, Wu Q, Lin Z. Sense and antisense expression of plasma membrane aquaporin BnPIP1 from Brassica napus in tobacco and its effects on plant drought resistance. Plant Sci. 2005;169:647–56.

    Article  CAS  Google Scholar 

  13. Perrone I, Gambino G, Chitarra W, Vitali M, Pagliarani C, Riccomagno N, Balestrini R, Kaldenhoff R, Uehlein N, Gribaudo I, Schubert A, Lovisolo C. The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well-watered conditions but not under water stress. Plant Physiol. 2012;160:965–77.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Ayadi M, Brini F, Masmoudi K. Overexpression of a wheat aquaporin gene, TdPIP2;1, enhances salt and drought tolerance in transgenic durum wheat cv. Maali. Int J Mol Sci. 2019;20(10):2389.

    Article  PubMed Central  CAS  Google Scholar 

  15. Tsuchihira A, Hanba YT, Kato N, Doi T, Kawazu T, Maeshima M. Effect of overexpression of radish plasma membrane aquaporins on water-use efficiency, photosynthesis and growth of Eucalyptus trees. Tree Physiol. 2010;30:417–30.

    Article  CAS  PubMed  Google Scholar 

  16. Aharon R, Shahak Y, Wininger S, Bendov R, Kapulnik Y, Galili G. Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress. Plant Cell. 2003;15:439–47.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Ayadi M, Cavez D, Miled N, Chaumont F, Masmoudi K. Identification and characterization of two plasma membrane aquaporins in durum wheat (Triticum turgidum L. subsp. durum) and their role in abiotic stress tolerance. Plant Physiol Bioch. 2011;49:1029–39.

    Article  CAS  Google Scholar 

  18. Peng Y, Arora R, Li G, Wang X, Fessehaie A. Rhododendron catawbiense plasma membrane intrinsic proteins are aquaporins, and their over-expression compromises constitutive freezing tolerance and cold acclimation ability of transgenic Arabidopsis plants. Plant Cell Environ. 2008;31:1275–89.

    Article  CAS  PubMed  Google Scholar 

  19. Li J, Yu G, Sun X, Liu Y, Liu J, Zhang X, Jia C, Pan H. AcPIP2, a plasma membrane intrinsic protein from halophyte Atriplex canescens, enhances plant growth rate and abiotic stress tolerance when overexpressed in Arabidopsis thaliana. Plant Cell Rep. 2015;34:1401–15.

    Article  CAS  PubMed  Google Scholar 

  20. Kirkham MB, Gardner WR, Gerloff GC. Regulation of cell division and cell enlargement by turgor pressure. Plant Physiol. 1972;49:961–2.

    Article  Google Scholar 

  21. Proctor SA, Minc N, Boudaoud A, Chang F. Contributions of turgor pressure, the contractile ring, and septum assembly to forces in cytokinesis in fission yeast. Curr Boil. 2012;22:1601–8.

    Article  CAS  Google Scholar 

  22. Ehlert C, Maurel C, Tardieu F, Simonneau T. Aquaporin-mediated reduction in maize root hydraulic conductivity impacts cell turgor and leaf elongation even without changing transpiration. Plant Physiol. 2009;150:1093–104.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Ortega JK, Munoz CM, Blakley SE, Truong JT, Ortega EL. Stiff mutant genes of phycomyces affect turgor pressure and wall mechanical properties to regulate elongation growth rate. Front Plant Sci. 2012;3:99.

    Article  PubMed  PubMed Central  Google Scholar 

  24. Kutschera U, Niklas KJ. Cell division and turgor-driven stem elongation in juvenile plants: a synthesis. Plant Sci. 2013;207:45–56.

    Article  CAS  PubMed  Google Scholar 

  25. Lockhart JA. An analysis of irreversible plant cell elongation. J Theor Boil. 1965;8:264–75.

    Article  CAS  Google Scholar 

  26. Leng H, Lu M, Wan X. Variation in embolism occurrence and repair along the stem in drought-stressed and re-watered seedlings of a poplar clone. Physiol Plantarum. 2013;147:329–39.

    Article  CAS  Google Scholar 

  27. Jiang C, Song X, He H, Chu L, Zhou H, Zhao Y, Xu Y, Zeng W, Lin X, Lu M. Genome-wide identification of plasma membrane aquaporin gene family in Populus and functional identification of PIP1;1 involved in osmotic stress. Environ Exp Bot. 2020;179:104200.

    Article  CAS  Google Scholar 

  28. Fetter K, Van WV, Moshelion M, Chaumont F. Interactions between plasma membrane aquaporins modulate their water channel activity. Plant Cell. 2004;16:215–28.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Srikanth A, Schmid M. Regulation of flowering time: all roads lead to Rome. Cell Mol Life Sci. 2011;68:2013–37.

    Article  CAS  PubMed  Google Scholar 

  30. Simpson GG, Dean C. Arabidopsis, the Rosetta stone of flowering time? Science. 2002;296:285–9.

    Article  CAS  PubMed  Google Scholar 

  31. Hukin D, Doering-Saad C, Thomas C, Pritchard J. Sensitivity of cell hydraulic conductivity to mercury is coincident with symplasmic isolation and expression of plasmalemma aquaporin genes in growing maize roots. Planta. 2002;215:1047–56.

    Article  CAS  PubMed  Google Scholar 

  32. Picaud S, Becq F, Dedaldechamp F, Ageorges A, Delrot S. Cloning and expression of two plasma membrane aquaporins expressed during the ripening of grape berry. Funct Plant Biol. 2003;30:621–30.

    Article  CAS  PubMed  Google Scholar 

  33. Matsuzaki Y, Ogawa-Ohnishi M, Mori A, Matsubayashi Y. Secreted peptide signals required for maintenance of root stem cell niche in Arabidopsis. Science. 2010;329:1065–7.

    Article  CAS  PubMed  Google Scholar 

  34. Zeuthen T. Molecular mechanisms for passive and active transport of water. Int Rev Cytol. 1995;160:99–161.

    Article  CAS  PubMed  Google Scholar 

  35. Proseus TE, Zhu GL, Boyer JS. Turgor, temperature and the growth of plant cells: using Chara corallina as a model system. J Exp Bot. 2000;51:1481–94.

    Article  CAS  PubMed  Google Scholar 

  36. Liu F, Quesada V, Crevillén P, Baürle I, Swiezewski S, Dean C. The Arabidopsis RNA-binding protein FCA requires a lysine-specific demethylase 1 homolog to downregulate FLC. Mol Cell. 2007;28:398–407.

    Article  PubMed  CAS  Google Scholar 

  37. Lim MH, Kim J, Kim YS, Chung KS, Seo YH, Lee I, Hong CB, Kim HJ, Park CM. A new Arabidopsis gene, FLK, encodes an RNA binding protein with K homology motifs and regulates flowering time via FLOWERING LOCUS C. Plant Cell. 2004;16:731–40.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Liu L, Zhang Y, Li Q, Sang Y, Mao J, Lian H, Wang L, Yang H. COP1-mediated ubiquitination of CONSTANS is implicated in cryptochrome regulation of flowering in Arabidopsis. Plant Cell. 2008;20:292–306.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Dieringer G. Variation in individual flowering time and reoproductive success of Agalinis strictifolia (Scrophulariaceae). Am J Bot. 1991;78:497–503.

    Article  Google Scholar 

  40. Ollerton J, Lack A. Relationships between flowering phenology, plant size and reproductive success in Lotus corniculatus (Fabaceae). Plant Ecol. 1998;139:35–47.

    Article  Google Scholar 

  41. Jang HY, Ahn SJ. Overexpression of Jatropha aquaporin genes, JcPIP1 and JcPIP2, does not alter response to salt and drought stresses in transgenic Arabidopsis. J Crop Sci Biotech. 2015;18:27–35.

    Article  Google Scholar 

  42. Wang X, Gao F, Bing J, Sun W, Feng X, Ma X, Zhou Y, Zhang G. Overexpression of the Jojoba aquaporin gene, ScPIP1, enhances drought and salt tolerance in transgenic Arabidopsis. Int J Mol Sci. 2019;20(1):153.

    Article  PubMed Central  CAS  Google Scholar 

  43. Jang JY, Lee SH, Rhee JY, Chung GC, Ahn SJ, Kang H. Transgenic Arabidopsis and tobacco plants overexpressing an aquaporin respond differently to various abiotic stresses. Plant Mol Biol. 2007;64:621–32.

    Article  CAS  PubMed  Google Scholar 

  44. Sreedharan S, Shekhawat UK, Ganapathi TR. Transgenic banana plants overexpressing a native plasma membrane aquaporin MusaPIP1;2 display high tolerance levels to different abiotic stresses. Plant Biotechnol J. 2013;11:942–52.

    Article  CAS  PubMed  Google Scholar 

  45. Sreedharan S, Shekhawat UK, Ganapathi TR. Constitutive and stress-inducible overexpression of a native aquaporin gene (MusaPIP2;6) in transgenic banana plants signals its pivotal role in salt tolerance. Plant Mol Biol. 2015;88:41–52.

    Article  CAS  PubMed  Google Scholar 

  46. Zhuo C, Wang T, Guo Z, Lu S. Overexpression of MfPIP2-7 from Medicago falcata promotes cold tolerance and growth under NO3 (-) deficiency in transgenic tobacco plants. BMC Plant Biol. 2016;16:138.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  47. Wang L, Zhang C, Wang Y, Wang Y, Yang C, Lu M, Wang C. Tamarix hispida aquaporin ThPIP2;5 confers salt and osmotic stress tolerance to transgenic Tamarix and Arabidopsis. Environ Exp Bot. 2018;152:158–66.

    Article  CAS  Google Scholar 

  48. Li S, Liu J, An Y, Cao Y, Liu Y, Zhang J, Geng J, Hu T, Yang P. MsPIP2;2, a novel aquaporin gene from Medicago sativa, confers salt tolerance in transgenic Arabidopsis. Environ Exp Bot. 2019;165:39–52.

    Article  CAS  Google Scholar 

  49. Zhang Y, Wang Z, Chai T, Wen Z, Zhang H. Indian mustard aquaporin improves drought and heavy-metal resistance in tobacco. Mol Biotechnol. 2008;40:280–92.

    Article  CAS  PubMed  Google Scholar 

  50. Kierzkowski D, Routier-Kierzkowska AL. Cellular basis of growth in plants: geometry matters. Curr Opin Plant Biol. 2019;47:56–63.

    Article  PubMed  Google Scholar 

  51. Aslam U, Khatoon A, Cheema HM, Bashir A. Identification and characterization of plasma membrane aquaporins isolated from fiber cells of Calotropis procera. J Zhejiang Univ-SC B. 2013;14:586–95.

    Article  CAS  Google Scholar 

  52. Tomos AD, Leigh RA, Shaw CA, Jones RGW. A comparison of methods for measuring turgor pressures and osmotic pressures of cells of red beet storage tissue. J Exp Bot. 1984;35:1675–83.

    Article  Google Scholar 

  53. Fricke W, Knipfer T. Plant Aquaporins and Cell Elongation. In Chaumont F, Tyerman DS, eds. Plant Aquaporins. Springer International Publishing. 2017; p. 107–131.

  54. Zhou H, Song X, Wei K, Zhao Y, Jiang C, Wang J, Tang F, Lu M. Growth-regulating factor 15 is required for leaf size control in Populus. Tree Physiol. 2019;39:381–90.

    Article  CAS  PubMed  Google Scholar 

  55. Kim JM, To TK, Ishida J, Morosawa T, Kawashima M, Matsui A, Toyoda T, Kimura H, Shinozaki K, Seki M. Alterations of lysine modifications on the histone H3 N-tail under drought stress conditions in Arabidopsis thaliana. Plant Cell Physiol. 2008;49:1580–8.

    Article  CAS  PubMed  Google Scholar 

  56. Clough SJ, Bent AF. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1998;16:735–43.

    Article  CAS  PubMed  Google Scholar 

  57. De Veylder L, Beeckman T, Beemster GT, Krols L, Terras F, Landrieu I, Schueren EVD, Maes S, Naudts M, Inzé D. Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis. Plant Cell. 2001;13:1653–68.

    Article  PubMed  PubMed Central  Google Scholar 

  58. Skirycz A, Claeys H, De-Bodt S, Oikawa A, Shinoda S, Andriankaja M, Maleux K, Eloy NB, Coppens F, Yoo S-D, Saito K, Inzé D. Pause-and-stop: the effects of osmotic stress on cell proliferation during early leaf development in Arabidopsis and a role for ethylene signaling in cell cycle arrest. Plant Cell. 2011;23:876–1888.

    Article  CAS  Google Scholar 

  59. Dolezel J, Greilhuber J, Suda J. Estimation of nuclear DNA content in plants using flow cytometry. Nat Protoc. 2007;2:2233–44.

    Article  CAS  PubMed  Google Scholar 

  60. Zhang C, Gong FC, Lambert GM, Galbraith DW. Cell type-specific characterization of nuclear DNA contents within complex tissues and organs. Plant Methods. 2005;1:7. https://doi.org/10.1186/1746-4811-1-7.

    Article  PubMed  PubMed Central  Google Scholar 

  61. Casamitjana-Martinez E, Hofhuis HF, Xu J, Liu CM, Heidstra R, Scheres B. Root-specific CLE19 overexpression and the sol1/2 suppressors implicate a CLV-like pathway in the control of Arabidopsis root meristem maintenance. Curr Biol. 2003;13:1435–41.

    Article  CAS  PubMed  Google Scholar 

  62. Saneoka H, Moghaieb REA, Premachandra GS, Fujita K. Nitrogen nutrition and water stress effects on cell membrane stability and leaf water relations in Agrostis palustris Huds. Environ Exp Bot. 2004;52:131–8.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

None.

Funding

This work was financially supported by the Basic Research Fund of RIF (RIF-2014–08) to XS, and of RIFNT (CAFYBB2018SZ019) to XW.

Author information

Authors and Affiliations

  1. Institute of New Forestry Technology, Chinese Academy of Forestry, Beijing, 100091, China

    Huani Leng & Xianchong Wan

  2. State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China

    Huani Leng, Cheng Jiang, Xueqin Song & Mengzhu Lu

  3. Zhejiang Agriculture & Forestry University, Hangzhou, 311300, China

    Cheng Jiang & Mengzhu Lu

  4. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Jiangsu, 210037, China

    Xueqin Song & Mengzhu Lu

Authors
  1. Huani Leng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cheng Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xueqin Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mengzhu Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xianchong Wan
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

H.L., X.W. and M.L. designed the study. H.L. and C.J. performed the experiments. H.L. and X.S. analyzed the data. H.L., M.L., X.W. and X.S. all participated in writing the manuscript. The author(s) read and approved the final manuscript.

Corresponding authors

Correspondence to Xueqin Song or Xianchong Wan.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interest

The authors declare no competing interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Leng, H., Jiang, C., Song, X. et al. Poplar aquaporin PIP1;1 promotes Arabidopsis growth and development. BMC Plant Biol 21, 253 (2021). https://doi.org/10.1186/s12870-021-03017-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s12870-021-03017-2

Keywords

BMC Plant Biology

ISSN: 1471-2229

Contact us