Fig. 2

Rootstock effect on global gene expression pattern revealed by RNA-sequencing. a Scatter plots comparing all expressed genes of scion stems on marang rootstocks vs. those on self-graft. Differentially expressed genes (FDR < 0.05 and |log₂FC|≥ 1) are donated in red or blue. b Heatmap and hierarchical clustering of differentially expressed genes among biologic replicates of graft combination. Heatmap was constructed with Heatmapper (http://www.heatmapper.ca/). Values of gene expression level are converted to Z-Score. C1-C3, replicates of self-graft (control); T1-T3, replicates of breadfruits on marang rootstocks. c Heatmap and hierarchical clustering of top major DEGs constructed by ClustVis (https://biit.cs.ut.ee/clustvis/). Colour codes represent standardized gene expression levels. Euclidean distance and Ward linkage were used to construct dendrograms against genes and samples. DEGs, differentially expressed genes