Skip to main content
Fig. 6 | BMC Plant Biology

Fig. 6

From: Lipases of germinating jojoba seeds efficiently hydrolyze triacylglycerols and wax esters and display wax ester-synthesizing activity

Fig. 6

WE-synthesizing activity of the jojoba seed lipases. a. Time course of WE synthesis from 18:1-TAG and 18:1-FA combined with 18:1-OH. Data represent mean values and error bars show the range of duplicates. Assay condition: aliquots (2.5 nmol of endogenous PC) of microsomal fraction mixture (isolated from two individual jojoba seeds from each accession, 35 dpg); substrates (20 nmol of [14C]18:1-FA or [14C]18:1-TAG and 20 nmol of fatty alcohol) added to freeze-dried microsomes in 19 μl benzene; benzene evaporation, and addition of 100 μl 0.1 M HEPES buffer (pH 7.0); incubation at 60 °C. b. WE synthesis from 18:1-FA and different fatty alcohols. Data represent mean values and error bars show the range of duplicates. Assays conditions: as above, 20 min incubation at 60 °C

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us