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Fig. 4 | BMC Plant Biology

Fig. 4

From: CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family alters arabinogalactan-protein glycosylation and function in Arabidopsis

Fig. 4

Multiplex gene-editing events occurred in three gRNAs of galt2 galt3 galt4 galt5 galt6 mutant line #29–36. (From top to bottom) The 3–3 gRNA generated a 97 bp deletion in GALT3; the 4–2 and 4–3 gRNAs created a 1 bp deletion and a 1 bp insertion in the GALT4 gene, respectively; the 6–3 gRNA produced a 4 bp indel mutation in the GALT6 gene. All mutations resulted in frame-shifts (red amino acids) and pre-mature stop codons (asterisks) in their respective protein sequences. Mutation sites are indicated by red arrows on the sequencing chromatograms on the right

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BMC Plant Biology

ISSN: 1471-2229

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