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LncRNA regulates tomato fruit cracking by coordinating gene expression via a hormone-redox-cell wall network

BMC Plant Biology volume 20, Article number: 162 (2020) Cite this article

Abstract

Background

Fruit cracking occurs easily under unsuitable environmental conditions and is one of the main types of damage that occurs in fruit production. It is widely accepted that plants have developed defence mechanisms and regulatory networks that respond to abiotic stress, which involves perceiving, integrating and responding to stress signals by modulating the expression of related genes. Fruit cracking is also a physiological disease caused by abiotic stress. It has been reported that a single or several genes may regulate fruit cracking. However, almost none of these reports have involved cracking regulatory networks.

Results

Here, RNA expression in 0 h, 8 h and 30 h saturated irrigation-treated fruits from two contrasting tomato genotypes, ‘LA1698’ (cracking-resistant, CR) and ‘LA2683’ (cracking-susceptible, CS), was analysed by mRNA and lncRNA sequencing. The GO pathways of the differentially expressed mRNAs were mainly enriched in the ‘hormone metabolic process’, ‘cell wall organization’, ‘oxidoreductase activity’ and ‘catalytic activity’ categories. According to the gene expression analysis, significantly differentially expressed genes included Solyc02g080530.3 (Peroxide, POD), Solyc01g008710.3 (Mannan endo-1,4-beta-mannosidase, MAN), Solyc08g077910.3 (Expanded, EXP), Solyc09g075330.3 (Pectinesterase, PE), Solyc07g055990.3 (Xyloglucan endotransglucosylase-hydrolase 7, XTH7), Solyc12g011030.2 (Xyloglucan endotransglucosylase-hydrolase 9, XTH9), Solyc10g080210.2 (Polygalacturonase-2, PG2), Solyc08g081010.2 (Gamma-glutamylcysteine synthetase, gamma-GCS), Solyc09g008720.2 (Ethylene receptor, ER), Solyc11g042560.2 (Ethylene-responsive transcription factor 4, ERF4) etc. In addition, the lncRNAs (XLOC_16662 and XLOC_033910, etc) regulated the expression of their neighbouring genes, and genes related to tomato cracking were selected to construct a lncRNA-mRNA network influencing tomato cracking.

Conclusions

This study provides insight into the responsive network for water-induced cracking in tomato fruit. Specifically, lncRNAs regulate the hormone-redox-cell wall network, including plant hormone (auxin, ethylene) and ROS (H2O2) signal transduction and many cell wall-related mRNAs (EXP, PG, XTH), as well as some lncRNAs (XLOC_16662 and XLOC_033910, etc.).

Background

Fruit cracking, one of the main disorders in fruit production, can easily cause adverse impacts in fruit marketability such as reducing fruit quality due to a poor appearance, decreasing shelf life, and even making the fruit unmarketable because of fungal infection [1].

Fruit cracking occurs easily under unsuitable environmental conditions. For instance, under abiotic stress caused by dry to very wet conditions, there will be a rapid flow into the fruit, and if the skin loses strength and elasticity due to factors such as maturation, cracking is most likely to occur [2]. It is widely accepted that plants have developed defence mechanisms and regulatory networks to respond to abiotic stress, which involve perceiving, integrating and responding to stress signals by modulating the expression of related genes [3,4,5,6,7,8]. Fruit cracking is also a kind of physiological disease caused by abiotic stress. Is there a regulatory network involved in fruit cracking?

Since the 1930s, researchers have performed many theoretical and practical studies on cracking [2, 9, 10]. Cracking is the result of a combination of internal and external factors. The internal factors are the fruit’s own characteristics (fruit size, shape, firmness, deposition of cutin, wax, strength of the pericarp, arrangement of cells in the pericarp, quantity and status of stomata, accumulation of osmoregulatory substances such as soluble sugars, growth stage of the fruit, etc.), while the external factors mainly include environmental factors (humidity, light, temperature, wind, etc.) and cultivation management measures (irrigation, mineral nutrition, plant regulation, etc.) [10,11,12,13]. Cortes [14] comprehensively analysed 62 genotypes and found that the correlation of cracked fruit with heredity was significantly greater than that with the environment, indicating that the cracking characteristic can show stable heritability and be regulated by certain genes.

Notably, cell wall components and modifications appear to be correlated with the strength of the skin and fruit cracking [15,16,17,18]. As ripening proceeds, cell wall degradation gradually occurs, and the fruit cracking rate increases [15, 16]. The cell wall is composed of a cellulose-hemicellulose (Cel-Hem) network and pectin, which is essential to maintain the mechanical strength of the cell wall. As the fruit matures, enzymes and proteins that degrade the polysaccharide components of the cell wall are produced, such as polygalacturonase (PG), extended protein (EXP), pectin methylesterase (PME), pectate lyases (PL), pectinase, β-galactosidase. (β-gal) and cellulase (Cx) [19,20,21,22]. The synergistic action of these enzymes leads to the degradation of cell wall polysaccharides and softening of the mature fruit peel [23]. Previous research has shown that genes such as EXP, PG, β-gal and XET are associated with fruit cracking [24,25,26,27,28,29]. Inhibition of β-gal gene expression increases the rate of fruit cracking [27]. In tomato, inhibition of LePG expression slightly reduces the rate of fruit cracking [28]. Simultaneous suppression of SlPG and SlEXP1 expression in ripening fruits reduces cell wall disassembly and thereby reduces the fruit cracking rate by approximately 12% [30]. It is not a single gene but many genes working together that regulate fruit cracking [30,31,32,33]. It remains unclear whether there are other genes related to fruit cracking and which one is the major gene.

NcRNAs (Non-coding RNA) are involved in a lot of life processes, such as cell growth, differentiation, proliferation, and apoptosis [34,35,36,37,38]. In contrast to the approximately 2% of protein-coding genes, more than 90% of genes do not have the ability to encode proteins and are transcribed into ncRNAs [34]. These ncRNAs were originally thought to represent “expression noise” or “expression waste”, but they have now been proven to be strictly regulated to play important roles in the biological processes of organisms and exhibit extremely complex biological functions [36,37,38]. While much of the published work on ncRNAs has been conducted in humans and animals, the studies on plants are limited to certain model plants, such as Arabidopsis, maize, and wheat [39, 40]. Xin [39] identified 125 stress-related lncRNAs (long non-coding RNA) in wheat, among which 71 responded to powdery mildew, and 77 responded to heat stress. Swiezewski [40] discovered that cold-induced long antisense intragenic RNA (COOLAIR) is involved in the vernalization process and regulates the expression of the plant flowering suppressor “gene flowering locus C” (FLC). Wang [41] reported the expression and evolution of lncRNAs in Solanaceae. Cui’s [42] results provided insights into the WRKY1 − lncRNA33732 − RBOH module involved in the regulation of H2O2 accumulation and resistance to pathogens in tomato. Wang [43] identified several lnRNAs that are involved in TYLCV infection by virus-induced gene silencing (VIGS) and genome-wide analysis. However, none of these studies have focused on lncRNAs and fruit cracking. Is it possible that lncRNAs play important roles in fruit cracking too?

This study aimed to obtain a global view of the transcriptional regulation (mRNAs and lncRNAs) of fruit cracking induced by irrigation in tomato. Differentially expressed mRNAs and lncRNAs related to fruit cracking were identified through transcriptome profiling and bioinformatic analysis. Finally, we determined a lncRNA-regulated hormone-redox-cell wall network for water-induced cracking in tomato. The findings reported here can increase our understanding of the transcriptional regulatory mechanisms of fruit cracking.

Results

RNA sequencing and identification of lncRNA and mRNA

In total, we obtained 0.81 to 1.14 billion raw reads and 0.79 to 1.14 billion clean reads from CR and CS tomatoes at various time points (0 h, 8 h and 30 h of saturated irrigation treatment) (Table 1). Through genomic comparison, Cufflinks splicing, and CPC2 and PFAM analysis, we identified 1 annotated lncRNA, 2508 putative lncRNAs, 33,784 annotated mRNAs and 409 novel mRNAs (Additional file 1).

Table 1 The overall assessment of the sequencing data
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Feature analysis of lncRNAs and identification of lncRNA-mRNA pairs

The average length of the obtained lncRNAs was 1470 nt, which was similar to that of the mRNAs (1221 nt); the average number of exons and average ORF length of the identified lncRNAs were 2.6 and 88.5 bp, which were much lower values than those for the mRNAs (4.7 and 347 bp) (Fig. 1), consistent with previous studies [44, 45]. At the same time, we used phyloP to separately score the lncRNAs and mRNAs, and the sequence conservation of the lncRNAs was lower than that of mRNAs, which was consistent with previous studies [46]. We identified 21,048 lncRNA-mRNA pairs with target relationships upstream and downstream of 2508 lncRNAs (Additional file 2).

Fig. 1
figure 1

Comparative features of mRNAs and lncRNAs. a Length distribution of mRNAs and lncRNAs. b Exon number distribution of mRNAs and lncRNAs. c ORF length distribution of mRNAs and lncRNAs. d Expression levels are indicated as log10 (FPKM + 1) values for the mRNAs and lncRNAs

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Differential expression analysis

Differentially expressed mRNAs and lncRNAs were analysed in CR and CS tomatoes by using edge R software (Fig. 2) and the number of differentially expressed genes was listed in the Additional file 3. mRNAs and lncRNAs with a Q-value< 0.05 and |log2 fold-change| > 1 were selected as differentially expressed genes.

Fig. 2
figure 2

Differentially expressed mRNAs and lncRNAs in different libraries. Upregulated (blue) and downregulated (red) mRNAs and lncRNAs were quantified. a mRNAs. b lncRNAs. Note: CS is cracking-susceptible, CR is cracking-resistant. The same abbreviations are used below

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Functional prediction of DEGs

To investigate the trends in gene functions and enrichment for DEGs, we performed GO (Gene Ontology) analysis of the selected mRNAs (Fig. 3; Additional file 4). The results showed that DEGs in the CR tomato were involved in a series of biological processes, such as regulation of biological process, biological regulation and regulation of cellular process, as well as catalytic activity. For the CS tomato, DEGs were mainly involved in single-organism metabolic process, biological process and catalytic activity. Between the CR and CS tomatoes, before irrigation treatment (0 h), DEGs were significantly enriched in oxidoreductase activity; after 8 h of irrigation treatment, there were some DEGs enriched in fruit ripening, anatomical structure maturation, and ageing; after 30 h of irrigation treatment, the number of DEGs enriched in the catalytic category was the highest, followed by the single-organism metabolic process and oxidation-reduction process categories, and cell components.

Fig. 3
figure 3

GO classification of transcripts of DEGs. The x-axis indicates number of DEGs, and the y-axis indicates the GO term

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To better understand the function of the DEGs, significantly enriched KEGG pathways were analysed (Fig. 4; Additional file 5). The results showed that the DEGs were mainly enriched in the ‘biosynthesis of secondary metabolites’, ‘cysteine and methionine metabolism’, ‘metabolic pathways’, ‘plant-pathogen interaction’, ‘photosynthesis-antenna protein’, ‘photosynthesis’, ‘histidine metabolism’ and ‘circadian rhythm-plant’ categories.

Fig. 4
figure 4

The top 20 KEGG pathways enriched by DEGs. The x-axis indicates the enrichment factor, and the y-axis indicates the pathway names

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DEGs (cell wall, redox, hormone related) regulate tomato fruit cracking

According to the gene expression analysis, 16 significantly differentially expressed genes (Additional file 6) were predicted to be related to fruit cracking in tomato, such as Solyc02g080530.3 (Peroxide, POD), Solyc01g008710.3 (Mannan endo-1,4-beta-mannosidase, MAN), Solyc08g077910.3 (Expanded, EXP), Solyc09g075330.3 (Pectinesterase, PE), Solyc07g055990.3 (Xyloglucan endotransglucosylase-hydrolase 7, XTH7), Solyc12g011030.2 (Xyloglucan endotransglucosylase-hydrolase 9, XTH9), Solyc10g080210.2 (Polygalacturonase-2, PG2), Solyc08g081010.2 (Gamma-glutamylcysteine synthetase, gamma-GCS), Solyc09g008720.2 (Ethylene receptor, ER), Solyc11g042560.2 (Ethylene-responsive transcription factor 4, ERF4) etc. (Table. 2 [47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70]). Hierarchical clustering analysis showed that the expression trends or levels of these genes in the two varieties were completely different after the irrigation treatment (Fig. 5a). For instance, the expression of XTH7, XTH9, PE and POD in the CR tomato showed a downward trend, while the expression in the CS tomato presented an upward trend. These genes play important roles in cell wall loosing and expansion. As disassembly of the fruit cell wall can influence fruit cracking [30], These plant cell-wall loosing genes may also play a key regulatory role in tomato fruit cracking. At the same time, we used Tomato Gene Expression Atlas (http://tea.solgenomics.net/expression_viewer/input) to verify the gene expression, and found that most differentially expressed genes in this experiment were expressed in tomato pericarp in red ripe stage. Among them, GCS, MAN and PG, the antioxidative genes and cell-wall degrading enzyme-associated genes showed the highest expression. Whereas high mRNA levels were never present in ERF. These might be because that the ERF is an upstream regulator [71], so it never presented a high expression in red ripe stage. The differences in gene expression in this experiment and Tomato Gene Expression Atlas might be due to different varieties, treatment and detection standards (Fig. 5b).

Table 2 Key genes related to tomato fruit cracking
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Fig. 5
figure 5

Hierarchical clustering analysis and proposed fruit cracking regulatory network. a Hierarchical clustering analysis showed the expression profiles of key genes involved in tomato fruit cracking. Based on the Euclidean distance, the minimum linkage method was used for cluster analysis. Boxes of the same colour represent the same gene in CS and CR. The solid dark circles represent genes that showed different expression trends in the two genotypes. b Gene expression cube in total pericarp of tomato during red ripen stage by using Tomato Gene Expression Atlas. c Predicted pathway diagram of fruit cracking in tomato, including hormones, reactive oxygen species, and cell wall polysaccharide metabolism. Solyc09g008720.2, ethylene receptor. Solyc11g042560.2, ethylene-responsive transcription factor. Solyc02g080530.3, peroxide. Solyc07g026650.3, 1-aminocyclopropane-1-carboxylate oxidase 5. Solyc07g055990.3, xyloglucan endotransglucosylase/ hydrolase 7. Solyc12g011030.3, xyloglucan endotransglucosylase/ hydrolase 9. Solyc08g077910.3, expansin. Solyc09g075330.3, pectinase. Solyc10g080210.2, polygalacturonase-2. Solyc09g075330.3, pectinesterase. XLOC_016662, XLOC_010878 and XLOC_033910 are predicted lncRNAs associated with the redox pathway. The dotted line indicates the speculation process

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Finally, we mapped a pathway diagram (Fig. 5c) of fruit cracking based on these differentially expressed lncRNAs, mRNAs and previous studies [71,72,73,74,75,76,77]. Within this pathway, ERF, POD, PG and PE play important roles. Previous researches suggests that ethylene influences fruit development and ripening (regulating cell wall-related PG and EXP gene expression) [71] and promotes programmed cell death of epithelial cells under ROS signalling [72]. Li et al. [73] showed that ARFs represent a point of cross-talk between ethylene and auxin signalling. Furthermore, auxin induces the production of ROS, and H2O2 decomposes polymers at the cell wall by producing ·OH [74]. Programmed cell death leads to a reduction in or loss of permeability of the plasma membrane, which in turn influences fruit cell activity, water absorption and cracking [75]. Simultaneously, the increase of auxin can promote the accumulation of H2O2 and the elongation of cells [76]. Furthermore, Rayle and Cleland [77] proposed the acid growth theory indicating that hydrogen ions may exert a purely chemical or physical effect, such as cleavage of acid-labile bonds on the wall, or they may activate normal enzymatic processes directly or indirectly, potentially leading to wall loosening. We analyzed the regulatory element in the promoter sequence of PG, PE, EXP and XTH7 and found that there are Ethylene-responsive element and auxin-responsive element. Based on these findings, we speculate that the regulatory network of fruit cracking, especially the coexpression of cell wall-, redox-, and hormone-related mRNAs and their corresponding lncRNAs, influences fruit cracking.

qRT-PCR validation of DEGs

Genes showing upregulated and the downregulated expression were randomly selected from the DEGs for qRT-PCR verification. The results of qRT-PCR revealed that most of these mRNAs shared similar expression tendencies to those indicated by the mRNA-Seq data, which can validate the reliability of our sequence data and our research results from the present study (Additional file 7). The expression levels detected by the two methods were slightly different, which might have been due to the different detection ranges and sensitivities of the two detection methods. The comparison of the relative expression measured by qRT-PCR and RNA-seq was showed in Additional file 8, R2>0.7 confirmed the reliability of the RNA-Seq analysis results.

Discussion

Tomato is one of the most popular commercial vegetables [78], however, its fruit shows high susceptibility to cracking [16, 31]. Cracks can occur throughout the fruit development stage during the ripening and post-harvest period [79, 80], which may cause serious economic losses. Different hypotheses have been presented to explain the occurrence of tomato fruit cracking. Previous studies have shown that rapid fruit swelling and fruit cracking are closely related [81]. Irregular temperatures or watering, especially a shift from a lower temperature to a much higher temperature or from extremely dry to very humid conditions, will lead to rapid swelling. The pressure of the rapidly expanding pulp on the peel may lead to fruit cracking [82]. Cell senescence and apoptosis also influence skin strength and water absorption, which can in turn affect fruit cracking [10]. In addition, a large differences between day and night temperatures can lead to the accumulation of carbohydrates [83]. Fruits with high levels of carbohydrates absorb more water, grow much faster and are more likely to crack [84]. In general, fruit cracking is a complex problem involving a mixture of genetics and the environment. Previous studies have suggested it is not a single gene but many genes that work together to regulate fruit cracking [31, 32].

Cell wall polysaccharide metabolic

The DEG Solyc08g077910.3 encodes an Expansin-like protein that breaks down the hydrogen bonds between molecules in the cell wall macromolecular network to promote the depolymerization of the network, which can lead to relaxation of the cell wall [52]. In this experiment, the expression level of Solyc08g077910.3 was increased significantly after 8 h of irrigation (log2 fold-change = 7.13395) in CS tomato. The increased expression of the expansin-like gene can relax the cell wall and may influence fruit cracking.

Solyc07g055990.3 and Solyc12g011030.2 encode xyloglucan endotransglucosylase/hydrolases 7 and 9, respectively, which mediate the cleavage and polymerization of β-1,4-xyloglucan in the primary cell wall. Xyloglucan is usually fused to the cell wall, and its oligosaccharides determine tissue tension [47]. Jan [48] found that OsXTH8 is involved in the cell wall modification process in rice and is highly expressed in the vascular bundle of the sheath and the young roots, in which the cells are rapidly elongated and differentiated. In addition, it can respond to gibberellin. He [49] found that OsXTH5, OsXTH19, OsXTH20, OsXTH24 and OsXTH28 play important roles in the elongation of rice peduncles and can respond to drought stress. These studies indicate that the OsXTH gene family plays an important role in the regulation of the structural function of rice cell walls. In this experiment, the expression levels of Solyc12g011030.2 and Solyc07g055990.3 in CS tomato showed an upward trend, while they showed a downward trend in the CR tomato (Fig. 5a). Simultaneously, the expression level in CS tomato was significantly higher than that in CR tomato. This illustrates that the CR tomato may exhibit a greater osmotic stress resistance ability with downregulation of the XTH gene that can strengthen the cell wall upon encountering water stress.

The DEG Solyc10g080210.2 (Polygalacturonase-2) can remove the methyl group from polygalacturonic acid; during tomato maturation, the degree of methylation decreases from 90% in the green ripen period to 35% in the red ripen period [50], which accelerates the degradation of the cell wall. In an antisense PaPG1 transgenic study of strawberry, the expression level of PG was significantly inhibited, and the degree of fruit softening was significantly delayed [51].

Redox processes

Previous studies have shown that peroxidase in the cell wall leads to cell wall sclerosis by causing cross-linking of cell wall components, thereby inhibiting cell elongation [56,57,58]. Peroxidase can also directly regulate plant cell elongation by controlling H2O2 levels [59]. Solyc02g080530.3 encodes peroxide, whose levels are significantly higher in CS tomato than in CR tomato. The expression of these genes in CS tomato fruits may increase cell wall hardness and hinder the elongation of the cell wall, which will lead to fruit cracking when water absorption swelling occurs. Solyc01g081250.3 encodes glutathione-S-transferase (GST). The GST superfamily enzymes exhibit multiple functions in plants. They are not only involved in primary metabolism and secondary metabolism [70], but they can also protect plants from oxidative damage and heterogeneous substances [60,61,62]. According to the data analysis, the gene expression of Solyc01g081250.3 in the CR tomato was significantly higher than that in the CS tomato after 0 h, 8 h and 30 h of irrigation treatment. Higher expression of GST in CR tomato can better maintain cell vigour and be beneficial to tomato fruits when coupled with water stress.

Hormone-related

Previous research has shown that hormones can regulate the expression of cell wall-related genes. Trainotti [63] studied the expression of 32 genes related to cell wall synthesis and degradation. Their research showed that the expression of these genes in fleshy fruits can be inhibited by ethylene, while ethylene promotes the expression of these genes during fruit ripening and softening. At the same time, ethylene inhibits and promotes dual regulatory effects on the formation of plant secondary metabolites [64,65,66]; TAPG1, encoding a cell wall-degrading enzyme, can be induced by ethylene at the transcriptional level in tomato [67]; Rose [68] showed that ethylene regulates LeEXP1, which is specifically expressed only during fruit ripening. The pathway of ethylene biosynthesis in plants is the methionine cycle [85,86,87]. In this study, KEGG functional analysis of DEGs revealed significant enrichment in the methionine metabolic pathway. Solyc11g042560.2 encodes an ethylene receptor, while Solyc09g008720.2 encodes an ethylene-responsive transcription factor, and their expression levels are significantly upregulated after irrigation and are higher in CS tomato than in CR tomato.

LncRNAs regulate tomato fruit cracking by coordinating gene expression in the hormone-redox-cell wall network

Liu’s research suggests that plants have gradually developed complex signalling pathways to cope with adverse environmental stimuli [8]. That is, plants perceive different stress signals from the circumstances in which they occur and then integrate these signals and respond to these different stresses by modulating the expression of related genes. Is it possible that cracking is also regulated by a complex network?

LncRNAs play important roles in epigenetic regulation, cell cycle regulation and many other activities. Here, we identified several lncRNAs that are involved in fruit cracking. Most lncRNAs are not annotated, and we do not know their functions. To predict the functions of these lncRNAs, we performed functional analysis of lncRNA-targeted mRNAs and constructed an lncRNA-mRNA network (Fig. 6; Additional file 9). The results showed that the mRNAs in the network (Fig. 6a) were mainly enriched in the ‘oxidation-reduction process’, ‘oxidoreductase activity’, ‘hormone metabolic process’, ‘response to hormone stimulus’, ‘catalytic activity’, ‘cell wall organization’ and ‘external encapsulating structure’ categories. We classified the target genes into four categories (cell wall polysaccharide metabolism, oxidation-reduction processes, hormones and others) based on their functions and amounts.

Fig. 6
figure 6

LncRNA-mRNA networks. The orange triangles represent lncRNAs, and the circles represent mRNAs (green: cell wall polysaccharide metabolism, yellow: redox process, blue: hormone-related, pink: other). The red edge represents the targeting mode of the lncRNA and the co-localized mRNA, and the grey edge represents co-expression. a The lncRNA-mRNA network influencing tomato fruit cracking. b LncRNAs that target the same kinds of mRNAs. c LncRNAs that target different kinds of mRNAs play an important role in the network

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Some lncRNAs specifically target functional mRNAs, and we can assume that the lncRNAs perform similar functions to their target mRNAs. For example, many of the target genes of XLOC_033910, XLOC_007053, and XLOC_008464 (Fig. 6b) are enriched in categories such as ‘dioxygenase activity’, ‘oxidation-reduction process’ and ‘oxidoreductase activity’, so we predicted their gene function as ‘redox regulation’.

Some lncRNAs are targets of significantly differentially expressed mRNAs with various functions. For example, for XLOC_033910 (Fig. 6c), the target genes (Solyc06g060070.3,Solyc07g014670.3, Solyc05g055590.2, etc.) are enriched in the ‘oxidoreductase activity’ and ‘dioxygenase activity’ terms. XLOC_008464 also has other target genes, such as Solyc03g123550.1 (‘response to hormone stimulus’), and Solyc11g005750.2 (‘cell wall’). Previous studies [58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80] have shown that redox, hormone and cell wall terms are all very important factors that can influence fruit cracking, so we speculate that lncRNA XLOC_7053 may plays an important role in regulating tomato fruit cracking.

Liao’s research strongly supported Ethylene-responsive factor 4 (ERF4) was associated with rind hardness and cracking resistance in watermelon fruits [88]. In our study, DEGs also included ethylene, as well as cell wall and redox related genes. Based on our research and previous research [67,68,69, 71,72,73,74,75,76,77], we mapped a “hormone-redox-cell wall” pathway diagram including lncRNA associated with fruit cracking.

Conclusions

In this study, the key genes involved in the response to tomato cracking were identified by high-throughput sequencing, which has important significance for guiding the selection of new tomato varieties. We have also established an lncRNA-mRNA (hormone-redox-cell wall) network to learn about the precise regulation of fruit cracking by lncRNAs. To the best of our knowledge, this is the first discovery of the lncRNA-mRNA network involved in tomato fruit cracking.

Methods

Plant materials and sample collection

RNA expression in 0 h, 8 h and 30 h irrigation-treated fruits from two contrasting tomato genotypes, ‘LA1698’ (cracking-resistant, CR) and ‘LA2683’ (cracking-susceptible, CS), was analysed by mRNA and lncRNA sequencing. ‘LA2683’ and ‘LA1698’ (Fig. 7) were both introduced by the Tomato Genetics Resource Centre (TGRC, University of California, Davis). The fruit cracking rates of ‘LA2683’ and ‘LA1698’ are 77.53 and 20.17% (Additional file 10), respectively. Both lines were selected and self-pollinated for more than 6 generations. All the seedlings were grown in 72-plug trays on 18 March 2016. On 28 April 2016, they were transplanted to the same greenhouse of the Kunshan Yuye Leaf Vegetable Base (31°95′E, 119°16′N), Suzhou, Jiangsu Province, China. The climate of this area belongs to the north subtropical south monsoon climate zone, with four distinct seasons and plenty of rainfall. The average annual temperature is 15.7 °C, and the annual average precipitation is 1094 mm according to China Weather Network (http://www.weather.com.cn/jiangsu). These two genotypes were planted side by side. Plant spacing followed a 30 × 50 × 100 cm pattern. Drip irrigation were adopted to guarantee the consistent of irrigation amount. Fertilization practices and pest control were those usually used by local growers. When fruits of the third cluster ripen, saturated irrigation based on previous researches [2, 16, 31] with slightly modificationwas adopted to induce fruit cracking. Specifically, plants were firstly irrigated using flood irrigation for 2 h. The field was essentially flooded with water which was allowed to totally soak into the soil. We then adopted drip irrigation to keep the soil saturated with water. After 0 h, 8 h and 30 h of irrigation treatment, for each genotype, treatment, one fruit were cut into four pieces from peduncle to blossom-end including exocarp and mesocarp without pulp. Each group has two repetitions. Samples of both genetypes were immediately frozen in liquid nitrogen and stored at − 80 °C. Sampling time is based on morphological observation. At 8 h, cracked fruits began to appear in cracking susceptible tomato ‘LA2683’ but not in cracking resistant tomato ‘LA1698’. At 30 h, the fruits of ‘LA2683’ severely cracked and fruits of ‘LA1698’ slightly cracked .

Fig. 7
figure 7

Experimental flow chart

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RNA-seq analysis

Twelve fruit samples (2 genotypes, 3 time points, each with two replications) were collected and sequenced by Novogene, Beijing, China. RNA was extracted from the tomato pericarp, and qualified RNA samples were used to construct a cDNA library. Transcriptome sequencing was carried out on the Illumina HiSeq 2500 platform. Sequences showing low quality, linker contamination or a high unknown base (N) content were filtered from the raw reads obtained after sequencing. The filtered high-quality clean data were aligned to the ITAG4.0 reference genome using Hisat2 [89], and the transcriptome was assembled by using Cufflinks [90].

Identification of mRNA and lncRNA

The transcripts that could be compared to known transcript data were identified as annotated mRNAs.

Then, the transcripts were screened according to the following criteria: (1) exon number ≥ 2 and (2) transcript length ≥ 200 bp. Additionally, (3) Transcripts that overlapped with the database annotation of the exon area according to Cuffcompare software were screened out. The lncRNAs overlapping with the exon region of a spliced transcript were included in the subsequent analysis. (4) The expression level of each transcript was calculated with Cuffquant, and transcripts with an FPKM≥0.5 were selected. (5) Two algorithms for evaluating protein-coding potential (CPC2 [91] and PFAM [92]) were used to predict the protein-coding potential of the remaining transcripts. Only when these two algorithms simultaneously indicated no protein-coding potential were the sequences considered to be predicted lncRNAs. Finally, the predicted lncRNAs were obtained. The transcripts showing the potential to encode proteins by “CPC2” and “PFAM” were identified as novel mRNAs (Fig. 8).

Fig. 8
figure 8

Transcript screening process

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Analysis of mRNAs and lncRNAs

The mRNA and lncRNA abundance of the unigenes was normalized via the fragments per kilobase of exon model per million mapped reads (FPKM) approach. The log2 fold changes between two samples were tested statistically to determine whether the expression of an individual gene was significantly altered. A Q-value < 0.05 and |log2 fold-change| > 1 was considered to indicate a differentially expressed gene (DEG). Analyses of mRNA and lncRNA expression trends in tomato fruit after irrigation were then carried out. To understand the function of differentially expressed mRNAs, these mRNAs were further subjected to GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis by using GOseq [93] and KOBAS [94] software, respectively.

Feature analysis of lncRNAs and identification of lncRNA-mRNA pairs

Genomic characterization of the predicted lncRNAs was performed and compared with the mRNA results. The parameters for comparison to understand the genomic characteristics of the lncRNAs included the number of exons, ORF length, transcript nucleic acid length and sequence conservation between species.

LncRNAs mainly acts on their protein-coding target genes by cis-regulation or trans-regulation to achieve their regulatory functions. One of the functions of lncRNAs is cis-regulation of their neighbouring genes of the same allele; lncRNAs, which were located in less than 100 kb up/down stream of a gene, probably acted as cis-regulators. The up-stream lncRNAs having intersection of promoter or other cis-elements may regulate gene expression during or after transcription. The 3’UTR region or down-stream lncRNAs may perform other regulatory functions [95]. Another function of lncRNAs is the trans-regulation of co-expressed genes that are not adjacent to lncRNAs. For trans-regulation analysis, multiple repetitions are required for the result to be accurate and persuasive, samples with a value < 6 are not recommended [96, 97]. So in this study, lncRNAs by cis-regulation were analyzed. LncRNA within 100 kb upstream and downstream of the mRNA was identified as the lncRNA-mRNA pair and the function of lncRNAs was predicted through functional enrichment analysis of the cis target gene.

qRT-PCR verification

Total RNA was extracted from tomato fruit by using the RNAprep Pure Plant Kit (Tiangen Biotech Co., Ltd. (Beijing, China)) following the manufacturer’s instructions. cDNA was generated using 5 μl of total RNA and abm’s 5X All-In-One MasterMix. qRT-PCR was performed in a CFX 96 Touch RT-PCR detection system (Bio-Rad, USA) with abm’s EvaGreen 2X qPCR MasterMix-Low ROX. Then, 9 DEGs were randomly selected from the DEGs to verify the RNA-seq results. Gene-specific primers were designed using Premier 5.0 [98] and actin as a reference gene [30]. The relative levels of gene expression were calculated using the 2-ΔΔCT method [99] . The sequences of the primers are listed in Additional file 11.

Availability of data and materials

The datasets generated during and/or analysed during the current study are included in this published article (and its supplementary information files). All RNA sequencing data from this study are available in the NCBI sequence read archive (SRA) under accession numbers: SAMN14409497, SAMN14409498, SAMN14409499, SAMN14409500, SAMN14409501, SAMN14409502, SAMN14409503, SAMN14409504, SAMN14409505, SAMN14409506, SAMN14409507 and SAMN14409508 (https://www.ncbi.nlm.nih.gov/sra/PRJNA613785).

Abbreviations

CS:

Cracking-susceptible

CR:

Cracking-resistant

CS 0 h:

Cracking-susceptible tomato fruit before irrigation (0 h)

CS 8 h:

Cracking-susceptible tomato fruit after 8 h of irrigation

CS 30 h:

Cracking-susceptible tomato fruit after 30 h of irrigation

CR 0 h:

Cracking-resistant tomato fruit before irrigation (0 h)

CR 8 h:

Cracking-resistant tomato fruit after 8 h of irrigation

CR 30 h:

Cracking-resistant tomato fruit after 30 h of irrigation

DEGs:

Differentially expressed genes

GO:

Gene Ontology

KEGG:

Kyoto Encyclopedia of Genes and Genomes

qRT-PCR:

Quantitative real-time PCR

FPKM:

Expected number of fragments per kilobase of transcript sequence per millions base pairs sequenced

References

  1. Khadivikhub A, Zamani Z, Bouzari N. Evaluation of genetic diversity in some Iranian and foreign sweet cherry cultivars by using RAPD molecular markers and morphological traits. Hortic Environ Biotechnol. 2008;49(3):188–96.

    CAS  Google Scholar 

  2. Balbontín C. Ayala, et al. cracking in sweet cherries: a comprehensive review from a physiological molecular and genomic perspective. Chilean J Agric Res. 2013;73(1):66–72.

    Article  Google Scholar 

  3. Mukhopadhyay A, Vij S, Tyagi AK. Overexpression of a zinc finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco. Proc Natl Acad Sci U S A. 2004;101:6309–14.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Kang MY, Fokar M, Abdelmageed H, Allen RD. Arabidopsis SAP5 functions as a positive regulator of stress responses and exhibits E3 ubiquitin ligase activity. Plant Mol Biol. 2011;75:451–66.

    Article  CAS  PubMed  Google Scholar 

  5. Wu X, Shiroto Y, Kishitani S, Ito Y, Toriyama K. Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter. Plant Cell Rep. 2009;28(1):21–30.

    Article  CAS  PubMed  Google Scholar 

  6. Sakuma Y, Maruyama K, Osakabe Y, et al. Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought responsive geneexpression. Plant Cell. 2006;18(5):1292–309.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Yamaguchi-Shinozaki K, Shinozaki K. Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters. Trends Plant Sci. 2005;10:88–94.

    Article  CAS  PubMed  Google Scholar 

  8. Liu JH, Peng T, Dai WS. Critical cis-acting elements and interacting transcription factors: key players associated with abiotic stress responses in plants. Plant Mol Biol Report. 2014;32:303–17..

    Article  CAS  Google Scholar 

  9. Sawada E. Studies on the cracking of cherries. I. the cause of cracking. Agric Hortic. 1931;6:964–892.

    Google Scholar 

  10. Khadivi-Khub A. Physiological and genetic factors influencing fruit cracking. Acta Physiol Plant. 2015;37:1718.

    Article  CAS  Google Scholar 

  11. Cristián B, Héctor A, Bastías RM, et al. Cracking in sweet cherries: a comprehensive review from a physiological, molecular, and genomic perspective. Chilean J Agric Res. 2013;73(1):66–72.

    Article  Google Scholar 

  12. Beyer M, Hahn R, Peschel S, Harz M, Knoche M. Analysing fruit shape in sweet cherry (Prunus avium L.). Sci Hortic. 2002;96:139–50.

    Article  Google Scholar 

  13. Considine J, Brown K. Physical aspects of fruit growth. Theoretical analysis of distribution of surface growth forces in relation to cracking and splitting. Plant Physiol. 1981;68:371–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Cortes C, Ayuso MC, Palomares G, Cuartero J, Nuez F. Relationship between radial and concentric cracking of tomato fruit. Sci Hortic. 1983;21:323–8.

    Article  Google Scholar 

  15. Domínguez E. Ferna’ndez MD, Herna’ndez JCL, et al. tomato fruit continues growing while ripening, affecting cuticle properties and cracking. Physiol Plant. 2012;146(4):473–86.

    Article  PubMed  CAS  Google Scholar 

  16. Emmons CLW, Scott JW. Environmental and physiological effects on cuticle cracking in tomato. J Am Soc Hortic Sci. 1997;122(6):797–801.

    Article  Google Scholar 

  17. López-Casado G, Matas AJ, Domínguez E, Cuartero J, Heredia A. Biomechanics of isolated tomato (Solanum lycopersicuml.) fruit cuticles: the role of the cutin matrix and polysaccharides. J Exp Bot. 2007;58:3875–83.

    Article  PubMed  CAS  Google Scholar 

  18. Domínguez E, España L, López-Casado G, Cuartero J, Heredia A. Biomechanics of isolated tomato (Solanum lycopersicum) fruit cuticles during ripening: the role of flavonoids. Funct Plant Biol. 2009;36:613–20.

    Article  PubMed  Google Scholar 

  19. Ruiz-May E, Rose JKC. Progress toward the tomato fruit cell wall proteome. Funct Plant Biol. 2013;29:7.

    Google Scholar 

  20. Carpita NC, Campbell M, Tierney M. Pectin: cell biology and prospects for functional analysis. Plant Cell Walls. 2001;2:9–27.

    Google Scholar 

  21. Marin-Rodriguez MC. Pectate lyases, cell wall degradation and fruit softening. J Exp Bot. 2002;53(377):2115–9.

    Article  CAS  PubMed  Google Scholar 

  22. Geitmann PA. Pectin and the role of the physical properties of the cell wall in pollen tube growth of Solanum chacoense. Planta. 2005;220(4):582–92.

    Article  PubMed  CAS  Google Scholar 

  23. Cantu D, Vicente A, Greve L, et al. The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea. Proc Natl Acad Sci U S A. 2008;105(3):859–64.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Wang Y, Lu WJ, Li JG, Jiang Y. Differential expression of two expansion genes in developing fruit of cracking-susceptible and resistant litchi cultivars. J Am Soc Hortic Sci. 2006;131(1):118–21.

    Article  CAS  Google Scholar 

  25. Kasai S, Hayama H, Kashimura Y, Kudo S, Osanai Y. Relationship between fruit cracking and expression of the expansin gene MdEXPA3 in ‘Fuji’ apples (Malus domesticaBorkh.). Sci Hortic. 2008;116(2):194–8.

    Article  CAS  Google Scholar 

  26. Huang XM, Wang HC, Lu XJ, et al. Cell wall modifications in the pericarp of litchi (Litchi chinensis Sonn.) cultivars that differ in their resistance tocracking. J Hortic Sci Biotechnol. 2006;81(2):231–7.

    Article  Google Scholar 

  27. Moctezuma E, Smith DL, Gross KC. Antisense suppression of a ß-galactosidase gene (TBG6) in tomato increases fruit cracking. J Exp Bot. 2003;54:2025–33.

    Article  CAS  PubMed  Google Scholar 

  28. Schuch W, Kanczler J, Robertson D, et al. Fruit quality characteristics of transgenic tomato fruit with altered polygalacturonase activity. Hortscience. 1991;26(12):1517–20.

    CAS  Google Scholar 

  29. Lu W, Wang Y, Jiang Y, et al. Differential expression of litchi XET genes in relation to fruit growth. Plant Physiol Bochemistry. 2006;44(11):707–13.

    Article  CAS  Google Scholar 

  30. Jiang FL, Lopez A, Jeon S, et al. Disassembly of the fruit cell wall by the ripening-associated polygalacturonase and expansin influences tomato cracking. Horticulture Res. 2019;6:17.

    Article  CAS  Google Scholar 

  31. Peet M. Fruit cracking in tomato. Hort Technol. 1992;2(2):216–23.

    Article  Google Scholar 

  32. Cuartero J, Palomares G, Balasch S. Tomato fruit cracking under plastic-house and in the open air. II. General and specific combining abilities. BMC Infect Dis. 1981;15(1):1–13.

    Google Scholar 

  33. Yang ZE, Wu Z, Zhang C, Hu EM, Zhou R, Jiang FL. The composition of pericarp, cell aging, and changes in water absorption in two tomato genotypes: mechanism, factors, and potential role in fruit cracking. Acta Physiol Plant. 2016;38:215.

    Article  CAS  Google Scholar 

  34. Birney E. Stamato yannopoulos JA, Dutta a, et al. Indentification and analysis of function elements in 1% of the human genome by the encode pilot project. Nature. 2007;447(7146):799–816.

    Article  CAS  PubMed  Google Scholar 

  35. Derrien T, Johnson R, Bussotti G, et al. The GENCODE v7 catalog of human long noncoding RNAs: analysis of their gene structure, evolution, and expression. Genome Res. 2012;22(9):1775–89.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Ponting CP, Belgard TG. Transcribed dark matter: meaning or myth? Hum Mol Genet. 2010;19(R2):R162–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Huttenhofer A, Schattner P, Polacek N. Non-coding RNAs: hope or hype? Trends Genet. 2005;21(5):289–97.

    Article  PubMed  CAS  Google Scholar 

  38. Wang JQ, Zhang YJ, Ren JM, et al. Advances in research on biological functions and significance of long non-coding RNA. Life Sci. 2012;24(06):543–8.

    Google Scholar 

  39. Xin MM, Wang Y, Yao YY, et al. Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing. BMC Plant Biol. 2011;11:61.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Swiezewski S, Liu F, Magusin A, Dean C. Cold-induced silencing by long antisense transcripts of an Arabidopsis Polycomb target. Nature. 2009;462(7274):799–802.

    Article  CAS  PubMed  Google Scholar 

  41. Wang X, Ai G, Zhang C, et al. Expression and diversification analysis reveals transposable elements play important roles in the origin of Lycopersicon-specific lncRNAs in tomato. New Phytol. 2016;209(4):1442–55.

  42. Cui J, Jiang N, Meng J, et al. LncRNA33732-respiratory burst oxidase module associated with WRKY1 in tomato- Phytophthora infestans interactions. Plant J. 2019;97(5):933–46.

    Article  CAS  PubMed  Google Scholar 

  43. Wang J, Yu W, Yang Y, et al. Genome-wide analysis of tomato long non-coding RNAs and identification as endogenous target mimic for microRNA in response to TYLCV infection. Sci Rep. 2015;5:16946.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. Consortium TF, Carninci P, Kasukawa T, et al. The transcriptional landscape of the mammalian genome. Science. 2005;309(5740):1559–63.

    Article  CAS  Google Scholar 

  45. Luo H, Yang H, Lin Y, et al. LncRNA and mRNA profiling during activation of tilapia macrophages by HSP70 and Streptococcus agalactiae antigen. Oncotarget. 2017;8(58):98455–70.

    PubMed  PubMed Central  Google Scholar 

  46. Johnsson P, Lipovich L, Dan G, Morris KV. Evolutionary conservation of long non-coding RNAs; sequence, structure, function. Biochim Biophys Acta. 2014;1840:1063–71.

    Article  CAS  PubMed  Google Scholar 

  47. Park YB, Cosgrove DJ. A revised architecture of primary cell walls based on biomechanical changes induced by substrate-specific endoglucanases. Plant Physiol. 2012;158:1933–43.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. Jan A, Yang GX, Nakamura H, et al. Characterization of a xyloglucan endotransglucosylase gene that is up-regulated by gibberellin in rice. Plant Physiol. 2004;136(3):3670–81.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  49. He H, Serraj R, Yang Q. Changes in OsXTH gene expression, ABA content, and peduncle elongation in rice subjected to drought at the reproductive stage. Acta Physiol Plant. 2009;31(4):749–56.

    Article  CAS  Google Scholar 

  50. Tieman DM, Harriman RW, Ramamohan G, Handa AK. An antisense pectin methylesterase Fene alters pectin chemistry and soluble solids in tomato fruit. Plant Cell. 1992;4(6):667–79.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  51. Quesada MA, Blanco-Portales R, Posé S, et al. Antisense down-regulation of the Fa PG1 gene reveals an unexpected central role for polygalacturonase in strawberry fruit softening. Plant Physiol. 2009;150:1022–32.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  52. McQueen-Mason S, Cosgrove DJ. Disruption of hydrogen bonding between plant cell wall polymers by proteins that induce wall extension. Proc Natl Acad Sci U S A. 1994;91(14):6574–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. Mo B, Bewley JD. The relationship between beta-mannosidase and endo-beta-mannanase activities in tomato seeds during and following germination: a comparison of seed populations and individual seeds. J Exp Bot. 2003;54(392):2503–10.

    Article  CAS  PubMed  Google Scholar 

  54. Stålbrand H, Saloheimo A, Vehmaanperä J, et al. Cloning and expression in Saccharomyces cerevisiae of a Trichoderma reesei beta-mannanase gene containing a cellulose binding domain. Appl Environ Microbiol. 1995;61(3):1090–7.

    Article  PubMed  PubMed Central  Google Scholar 

  55. Mohammad I, Sumit G, Meli VS, et al. Fruit ripening regulation of α-Mannosidase expression by the MADS box transcription factor RIPENING INHIBITOR and ethylene. Front Plant Sci. 2016;7:10.

  56. Andrews J, Adams SR, Burton KS, Edmondson RN. Partial purification of tomato fruit peroxidase and its effect on mechanical properties of tomato fruit skin. J Exp Bot. 2002;53:2393–9.

    Article  CAS  PubMed  Google Scholar 

  57. Cordoba-Pedregosa C, Cordoba F, Villalba JM, Gonzaez-Reyes JA. Zonal changes in ascorbate and hydrogen peroxide contents, peroxidase, and ascorbate-related enzyme activities in onion roots. Plant Physiol. 2003;131:697–706.

    Article  CAS  Google Scholar 

  58. Lin CC, Kao CH. Cell wall peroxidase against ferulic acid lignin, and NaCl-reddued root growth of rice seeding. J Plant Physiol. 2001;158:667–71.

    Article  CAS  Google Scholar 

  59. Passardi F, Cosio C, Penel C, Dunand C. Peroxidases have more functions than a Swiss army knife. Plant Cell Rep. 2005;24:255–65.

    Article  CAS  PubMed  Google Scholar 

  60. Kampranis SC, Damianova R, Atallah M, et al. A novel plant glutathione S-transferase-peroxidase suppresses bax lethality in yeast. J Biol Chem. 2000;275(38):29207–16.

    Article  CAS  PubMed  Google Scholar 

  61. Marrs KA, Alfenito M, Lloyd AM, Walbot V. A glutathioneS-transferase involved in vacuolar transfer encoded bythe maize gene Bronze-2. Nature. 1995;375(6530):397–400.

    Article  CAS  PubMed  Google Scholar 

  62. Bartling D, Radzio R, Steiner U, Weiler EW. A glutathione S-transferase with glutathione-peroxidase activity from Ara-bidopsis thaliana. Eur J Biochem. 1993;216(2):579–86.

    Article  CAS  PubMed  Google Scholar 

  63. Trainotti L, Zanin D, Casadoro G. A cell wall-oriented genomic approach reveals a new and unexpected complexity of the softening in peaches. J Exp Bot. 2003;54:1821–32.

    Article  CAS  PubMed  Google Scholar 

  64. Ruther J, Kleier S. Plant-plant signaling: ethylene synergizes volatile emission in Zea mays induced by exposure to (Z)-3-hexen-1-ol. J Chem Ecol. 2005;31:2217–22.

    Article  CAS  PubMed  Google Scholar 

  65. Hossain MA, Kim S, Kim KH, Lee SJ, Lee H. Flavonoid compounds are enriched in lemon balm (Melissa officinalis) leaves by a high level of sucrose and confer increased antioxidant activity. Hortscience. 2009;44:1907–13.

    Article  Google Scholar 

  66. Jeong SW, Das PK, Jeoung SC, et al. Ethylene suppression of sugar-induced anthocyanin pigmentation in Arabidopsis. Plant Physiol. 2010;154:1514–31.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  67. Kalaitzis P, Koehler SM, Tucker ML. Cloning of a tomato polygalacturonase expressed in abscission. Plant Mol Biol. 1995;28:647–56.

    Article  CAS  PubMed  Google Scholar 

  68. Rose JK, Cosgrove DJ, Albersheim P, Darvill AG, Bennett AB. Detection of expansin proteins and activity duringtomato fruit ontogeny. Plant Physiol. 2000;123(4):1583–92.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  69. Yoshida S, Tamaoki M, Ioki M, et al. Ethylene and salicylic acid control glutathione biosynthesis in ozone-exposed Arabidopsis thaliana. Physiol Plant. 2009;136(3):284–98.

    Article  CAS  PubMed  Google Scholar 

  70. Jablonkai I, Hatzios KK. In vitro conjugation of chloroacetanilide herbicides and atrazine with thiols and contribution of nonenzymatic conjugation to their glutathione mediated metabolism in corn. J Agric Food Chem. 1993;41(10):1736–42.

    Article  CAS  Google Scholar 

  71. Gao J, Zhang YX, Li ZG, Liu MC. Role of ethylene response factors (ERFs) in fruit ripening. Food Qual Saf. 2020;275:1–5.

  72. Steffens B, Kovalev A, Gorb SN, Sauter M. Emerging roots Alter epidermal cell fate through mechanical and reactive oxygen species signaling. Plant Cell. 2012;24(8):3296–306.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  73. Li JS, Dai XH, Zhao YD. A role for auxin response factor 19 in auxin and ethylene signaling in Arabidopsis. Plant Physiol. 2006;140(3):899–908.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  74. Liszkay A. Production of reactive oxygen intermediates (O2 middle dot-, H2O2, and middle dotOH) by maize roots and their role in wall loosening and elongation growth. Plant Physiol. 2004;136(2):3114–23.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  75. Wang BM, Ding GX, Wang XY, et al. Changes of histological structure and water potential of huping jujube fruit cracking. Sci Agric Sin. 2013;46(21):4558–68.

    CAS  Google Scholar 

  76. Ivanchenko MG, den Os D, Monshausen GB, Dubrovsky JG, Bednárová A, Krishnan N. Auxin increases the hydrogen peroxide (H2O2) concentration in tomato (Solanum lycopersicum) root tips while inhibiting root growth. Ann Bot. 2013;112(6):1107–16.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  77. Rayle DL, Cleland R. Enhancement of wall loosening and elongation by acid solutions. Plant Physiol. 1970;46(2):250–3.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  78. Nuruddin MM, Madramootoo CA, Dodds GT. Effects of water stress at different growth stages on greenhouse tomato yield and quality. Hort Science. 2003;38(7):1389–93.

    Article  Google Scholar 

  79. Walter JM. Hereditary resistance to disease in tomato. Annu Rev Phytopathol. 1967;5:131–62.

    Article  Google Scholar 

  80. Cotner SD, Burns EE, Leeper PW. Pericarp anatomy of crackresistant and susceptible tomato fruits. J Am Soc Hortic Sci. 1969;94:136–7.

    Google Scholar 

  81. Capel C, Yuste-Lisbona FJ, López-Casado G, et al. Multi-environment QTL mapping reveals genetic architecture of fruit cracking in a tomato RIL Solanum lycopersicum×S. pimpinellifolium population. Tag.theoretical & Applied Genetics. theoretische Und Angewandte Genetik. 2016;130(1):1–10.

    Google Scholar 

  82. Garcia-Luis A, Duarte AMM, Porras I, et al. Fruit splitting in “Nova” hybrid mandarin in relation to the anatomy of the fruit and fruit set treatments. Sci Hortic. 1994;57:215–31.

    Article  Google Scholar 

  83. Wang SY, Camp MJ. Temperatures after bloom affect plant growth and fruit quality of strawberry. Sci Hortic. 2000;85:183–99.

    Article  Google Scholar 

  84. Young HW. Inheritance of fruit cracking. Tomato Genet Coop. 1958;8:38.

    Google Scholar 

  85. Lieberman M, Kunishi A, Mapson LW, Wardale DA. Stimulation of ethylene production in apple tissue slices by methionine. Plant Physiol. 1966;41:76–82.

    Article  Google Scholar 

  86. Yang SF, Hoffman NE. Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol. 1984;35:155–89.

    Article  CAS  Google Scholar 

  87. Wang KL-C, Li H, Ecker JR. Ethylene biosynthesis and signaling networks. Plant Cell. 2002;14:S131–51.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  88. Liao NQ, Hu ZY, Li YY, et al. Ethylene-responsive factor 4 is associated with the desirable rind hardness trait conferring cracking resistance in fresh fruits of watermelon. Plant Biotechnol J. 2020;18(4):1066–77.

  89. Kim D, Langmead B, Salzberg SL. HISAT: a fast spliced aligner with low memory requirements. Nat Methods. 2015;12(4):357–60.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  90. Trapnell C, Williams BA, Pertea G, et al. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010;28:511–5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  91. Kang YJ, Yang DC, Kong L, et al. CPC2: a fast and accurate coding potential calculator based on sequence intrinsic features. Nuc Acids Res. 2017;45(W1):12–6.

  92. Finn RD, Tate J, Mistry J, et al. The Pfam protein families database. Nucleic Acids Res. 2008;32(1):D138.

    Google Scholar 

  93. Young MD, Wakefield MJ, Smyth GK. goseq: Gene Ontology testing for RNA-seq datasets; 2012.

    Google Scholar 

  94. Mao XZ, Cai T, Olyarchuk JG, Wei LP. Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary. Bioinformatics. 2005;21:3787–93.

    Article  CAS  PubMed  Google Scholar 

  95. Guli S, Esteller M. Cis-acting noncoding RNAs: friends and foes. Nat Struct Mol Biol. 2012;19:1068–75.

    Article  CAS  Google Scholar 

  96. Zhan SY, Dong Y, Zhao W, et al. Genome-wide identification and characterization of long non-coding RNAs in developmental skeletal muscle of fetal goat. BMC Genomics. 2016;17(1):666.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  97. Yang B, Xia ZA, Zhong B, et al. Distinct hippocampal expression profiles of long non-coding RNAs in an alzheimer’s disease model. Mol Neurobiol. 2017;54(7):4833–46.

  98. Lalitha S. Primer Premier 5. Biotech Softw Internet Rep. 2000;1(6):270–2.

    Article  Google Scholar 

  99. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 2001;25(4):402–8.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

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Funding

The work was supported by grants from the National Natural Science Foundation of China (No. 31701924), the Fundamental Research Funds for the Central Universities (No. KJQN201814, KYZZ201909) and Priority Academic Program Development of Jiangsu Higher Education Institutions. The funders provided the financial but played no role in the study design, analysis of the data and in writing the manuscript.

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Authors and Affiliations

  1. College of Horticulture, Nanjing Agricultural University, Weigang NO 1, Nanjing, 210095, Xuanwu District, China

    Lingzi Xue, Zhen Wu, Lu Yu & Fangling Jiang

  2. Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China, Ministry of Agriculture, Nanjing, 210095, China

    Lingzi Xue, Zhen Wu, Lu Yu & Fangling Jiang

  3. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun South St, Beijing, 10081, Haidian District, China

    Mintao Sun

  4. Institute of Vegetables, Xinjiang Academy of Agricultural Sciences, Nanchang Road 403, Urumchi, 830091, Shayibake District, China

    Qinghui Yu & Yaping Tang

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Contributions

JFL led and coordinated the project. XLZ and SMT collected the plant materials. YL and XLZ isolated the RNA. SMT and TYP conducted real-time quantitative PCR. XLZ, WZ and YQH conducted the bioinformatics analysis. XLZ, SMT, and JFL wrote the paper. JFL is the corresponding author and is responsible for all contact and correspondence. All authors have read and approved the final manuscript.

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Correspondence to Fangling Jiang.

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Supplementary information

Additional file 1: Table S1.

LncRNAs in 12 tomato fruit libraries.

Additional file 2: Table S2.

LncRNA-mRNA pairs were identified by cis-regulation.

Additional file 3: Table S3.

Number of differentially expressed mRNAs and lncRNAs in different libraries.

Additional file 4: Table S4.

GO analysis of DEGs between groups.

Additional file 5: Table S5.

KEGG analysis of DEGs between groups.

Additional file 6: Table S6.

Key genes related to tomato fruit cracking.

Additional file 7: Figure S1.

Real-time PCR validation of high-throughput sequencing data. The x-axis represents the different time points of sampling, the left y-axis represents relative expression levels, and the right y-axis represents FPKM values. Blue bars represent data yielded by qRT-PCR, and red points represent data obtained by RNA sequencing. Different letters indicate significant differences (P < 0.05). (a) LA2683, (b) LA1698.

Additional file 8: Figure S2.

The comparison of the relative expression measured by qRT-PCR and RNA-seq.

Additional file 9: Table S7.

Data for the lncRNA-mRNA network.

Additional file 10: Table S8.

Statistics for tomato cracking rate of ‘LA2683’ and ‘LA1698’.

Additional file 11: Table S9.

Detailed primer sequences for qRT-PCR.

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Xue, L., Sun, M., Wu, Z. et al. LncRNA regulates tomato fruit cracking by coordinating gene expression via a hormone-redox-cell wall network. BMC Plant Biol 20, 162 (2020). https://doi.org/10.1186/s12870-020-02373-9

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  • DOI: https://doi.org/10.1186/s12870-020-02373-9

Keywords

  • Tomato
  • LncRNA
  • mRNA
  • Transcriptome
  • Network
  • Fruit cracking

BMC Plant Biology

ISSN: 1471-2229

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