- Research article
- Open Access
Comparative transcriptome analysis reveals the molecular regulation underlying the adaptive mechanism of cherry (Cerasus pseudocerasus Lindl.) to shelter covering
BMC Plant Biology volume 20, Article number: 27 (2020)
Rain-shelter covering is widely applied during cherry fruit development in subtropical monsoon climates with the aim of decreasing the dropping and cracking of fruit caused by excessive rainfall. Under rain-shelter covering, the characteristics of the leaves and fruit of the cherry plant may adapt to the changes in the microclimate. However, the molecular mechanism underlying such adaptation remains unclear, although clarifying it may be helpful for improving the yield and quality of cherry under rain-shelter covering.
To better understand the regulation and adaptive mechanism of cherry under rain-shelter covering, 38,621 and 3584 differentially expressed genes were identified with a combination of Illumina HiSeq and single-molecule real-time sequencing in leaves and fruits, respectively, at three developmental stages. Among these, key genes, such as those encoding photosynthetic-antenna proteins (Lhca and Lhcb) and photosynthetic electron transporters (PsbP, PsbR, PsbY, and PetF), were up-regulated following the application of rain-shelter covering, leading to increased efficiency of light utilization. The mRNA levels of genes involved in carbon fixation, namely, rbcL and rbcS, were clearly increased compared with those under shelter-free conditions, resulting in improved CO2 utilization. Furthermore, the transcription levels of genes involved in chlorophyll (hemA, hemN, and chlH) and carotenoid synthesis (crtB, PDS, crtISO, and lcyB) in the sheltered leaves peaked earlier than those in the unsheltered leaves, thereby promoting organic matter accumulation in leaves. Remarkably, the expression levels of key genes involved in the metabolic pathways of phenylpropanoid (PAL, C4H, and 4CL) and flavonoid (CHS, CHI, F3’H, DFR, and ANS) in the sheltered fruits were also up-regulated earlier than of those in the unsheltered fruits, conducive to an increase in anthocyanin content in the fruits.
According to the physiological indicators and transcriptional expression levels of the related genes, the adaptive regulation mechanism of cherry plants was systematically revealed. These findings can help understand the effect of rain-shelter covering on Chinese cherry cultivation in rainy regions.
Cherry is a popular spring–summer fruit because of its early maturity and delicious taste, along with its antioxidant properties . Cherry trees prefer environments that are sunny and without stagnant water . During fruit development, they are vulnerable to the weather, such as rain and hail, which are the main causes of the severe dropping and cracking of fruit .
Colorless rain-shelter coverings for fruit crops such as grapes  and apples  are increasingly being used worldwide, and this may significantly enhance fruit yield , while having no obvious adverse effect on fruit quality . However, rain-shelter covering may unavoidably alter the microclimate of the canopy, leading to a reduction in the photosynthetically active radiation (PAR) , which is regarded as a key constraint for vegetative growth and fruit development . Therefore, flexible adjustment between light energy utilization and photoprotection is critical for plant performance and field adaptability under changing light conditions .
Recently, the adaptability of plants to weak light and shade conditions was documented chiefly in terms of physiological adjustment in many species, such as rubber tree , cotton , and sweet cherry . It is generally accepted that the shift in the physiological adaptability of plants to adverse conditions can ultimately be attributed to alterations at the molecular level. To date, the adaptive regulatory mechanisms of plants under variable light and/or temperature conditions have been analyzed using transcriptomic strategies in many crops; for example, differentially expressed genes (DEGs) in maize were mainly involved in most assimilation processes (photosynthesis and carbon fixation pathways), namely, photosynthetic light capture via the modification of chlorophyll (Chl) biosynthesis, suggesting the complex regulatory mechanisms and interactions between cold and light signaling processes . Under low-light conditions, the expression of photosystem I and II complex and electron transport-related genes increased in low-light-tolerant rice varieties; thus, the accelerated expression of photosynthesis-related genes under low light conditions contributed to the maintenance of rice yield . In addition, under low light conditions, leaves need to maintain effectively antioxidant capacity to maintain their carbohydrate production level , which was enough to prove that adjustment or adaptation of leaves to low light conditions (hours or days) was achieved by integrating multiple signals, as shown by a study conducted on Arabidopsis thaliana . Although the leaf adaptation to short-term light change had been considerably studied, the physiological and molecular responses of leaves to long-term weak light had not yet been unraveled so far.
Anthocyanin biosynthesis and accumulation in the fruit peel were regulated by the light intensity . Owing to the frequent rain in spring, anthocyanin biosynthesis was severely inhibited by light loss of 8–22% , resulting in the reduction in the anthocyanin content. The flavonoid and phenylpropanoid pathways played key roles in pigment synthesis in strawberry fruit . The structural genes for anthocyanin biosynthesis, such as PAL, CHS, CHI, F3H, DFR, ANS, and UFGT, had been reported in sweet cherry . Under shelter covering, previous studies have only focused on the interaction between fruit anthocyanin contents and the transcription of related genes in grape . However, to date, however, the molecular regulation of sheltered covering on the gene expression profiles of flavonoid and/or phenylpropanoid pathway has not yet been elucidated, which may be helpful for better understanding the anthocyanin accumulation.
Chinese cherry (Cerasus pseudocerasus Lindl.) is widely grown in Southwest China, which is characterized as a region with a subtropical monsoon, with frequent rain and hail in spring, severely constraining the expansion of the cherry industry. Previously, the use of shelter covering was substantially justified to contribute to the total Pn accumulation and fruit yield elevation, and to cause no obvious adverse effects on the vegetative growth and fruit quality of cherry . To fully understand the adaptability and regulatory mechanism of cherry on microclimatic changes under shelter covering, the objectives of present study were as the following: 1) to discover the DEGs upon shelter covering via transcriptomic analysis; 2) to identify the key genes involved in growth and development; and 3) to reveal the regulatory network involved in light-harvesting, photosynthetic electronic transport, and the antioxidant system etc., which can strongly facilitate the innovative improvement of plantation systems of this fruit crop.
Overview of the single-molecule real-time (SMRT) sequencing
To obtain the gene expression profiles under sheltered and unsheltered conditions, SMRT sequencing and Illumina RNA-Seq were conducted for leaves (Fig. 1a) and fruits (Fig. 1b) on the 35th, 45th, and 55th days after flowering (DAF35, DAF45, and DAF55). In total 1,048,866 post-filter polymerase reads (21.55G) were scanned. The subreads from the same polymerase read sequence generated a circular consistent sequence (CCS), which yielded 685,339 CCS sequences. Among them, 542,795 full-length non-chimera (FLNC) sequences with 5′-primer, 3′-primer, and poly-A were obtained, including 79.20% of all CCSs being FLNCs (300–22,293 bp). This proportion varied slightly between the two tissues, at 34.96% in the leaf data set and 44.25% in the fruit data set. The mean length of the FLNC reads is presented in Additional file 2: Table S1, and the length distribution of the FLNC reads is shown in Additional file 1: Figure S1. The FLNC length of leaf and fruit libraries of > 2 kb accounted for 63.95 and 65.21% of the corresponding FLNC, respectively. Comparison between the protein-coding gene transcripts and those of FLNC revealed a strong concordance, which exhibited a better recovery of large transcripts than previous Illumina RNA-Seq data for gene model prediction, particularly in the size range of 2500–4000-bp size range .
Annotation and functional classification of unigenes
The functions of unigenes were annotated by BLAST comparison and were predicted by comparative analysis with five databases [NCBI Nonredundant Protein Sequences (NR), Clusters of Orthologous Groups of Proteins (COG), a manually annotated and reviewed protein sequence database (Swiss-Prot), Kyoto Encyclopedia of Genes and Genomics (KEGG), Gene Ontology (GO)]. Among the 45,825 unigenes distributed to each of the databases, 45,747 (97.35% of the total) for Nr, 28,481 (60.61%) for COG, 38,629 (82.20%) for SwissProt, 18,202 (38.73%) for KEGG, and 26,065 (55.46%) for GO were investigated (Fig. 2a).
Analysis of the Nr database indicated that the highest homologies of cherry were with Prunus mume or with P. persica, with 22,919 (50.09%) and 18,545 (40.53%) unigenes annotated, respectively. In total, 28,481 annotated unigenes were classified into 37 functional groups of the three GO main categories: nine groups for molecular function (MF), 18 for biological process (BP), and 10 for cellular component (CC) (Fig. 2b, Additional file 4: Table S3). The top three GO terms for the classified genes were “protein binding” (4812), “ATP binding” (3907), and “protein kinase activity” (2272) for MF; “protein phosphorylation” (2273), “oxidation-reduction process” (1833), and “signal transduction” (1255) for BP; and “membrane” (1656), “integral component of membrane” (1571), and “nucleus” (748) for CC.
In total, 18,202 unigenes were mapped into 265 KEGG database pathways. The top three KEGG pathways were “Metabolism, Carbohydrate Metabolism” (1719); “Environmental Information Processing, Folding, Sorting, and Degradation” (1563); and “Genetic Information Processing, Translation” (1491) (Fig. 2c, Additional file 5: Table S4). The maps representing the highest number of unigenes included those of carbon metabolism (map 01200), RNA transport (map 03013), biosynthesis of amino acids (map 01230), protein processing in endoplasmic reticulum (map 04141), Spliceosome (map 03040), and starch and sucrose metabolism (map 00500).
Functional classification of DEGs under sheltered covering
After filtering the low-quality reads, 17.25 billion clean reads were acquired by Illumina RNA-Seq (Additional file 6: Table S5). According to the Illumina data, pair-wise comparisons (SL vs. UL, SF vs. UF) of gene expression among the three stages were performed. In response to microclimatic change with low-PAR conditions, in total, 38,621 (SL35 vs. UL35, SL45 vs. UL45, and SL55 vs. UL55) and 3584 (SF35 vs. UF35, SF45 vs. UF45, and SF55 vs. UF55) DEGs were detected from the GO database. Additionally, 38,621 DEGs for SL45 vs. UL45 and 2871 DEGs for SF45 vs. UF45 were acquired (Fig. 3a). Throughout the three developmental stages, a total of 6911 and 1755 DEGs were detected from the KEGG database in leaves and fruits; 6868 and 1552 DEGs for SL45 vs. UL45 and SF45 vs. UF45 were obtained, respectively (Fig. 3b). To assess the adaptation of leaves and fruits to the sheltered covering, the overall changes in biochemical pathways were analyzed using MapMan (Additional file 7: Table S6), including the DEGs of SL vs. UL and SF vs. UF at DAF45 (Fig. 3c, d). The overall changes in the biochemical pathway of these DEGs were shown by MapMan annotation. The metabolic activities of the leaves under the sheltered covering were greatly increased at DAF45, and the DEGs were assigned to different functional categories, such as photosynthesis, secondary metabolism, coenzyme metabolism, RNA biosynthesis, etc. (Fig. 3c). Under shelter covering for 45 days, the DEGs of fruit characterized by the up-regulation of secondary metabolism, cell wall organization, amino acid metabolism, etc. (Fig. 3d).
Compared with UL and UF, the up-regulated genes from GO terms of SL were significantly enriched in “catalytic activity,” “biological process,” “oxidoreductase activity,” and “oxidation-reduction process,” among others (Fig. 4a). The up-regulated genes of SF were primarily associated with “catalytic activity,” “biological process,” “oxidoreductase activity,” and “oxidation-reduction process,” among others, at DAF45 (Fig. 4b). Up-regulated genes from KEGG terms of SL in contrast to UL included those associated with “circadian rhythm-plant,” “glyoxylate and dicarboxylate metabolism,” “porphyrin and chlorophyll metabolism,” and “carbon fixation in photosynthetic organisms,” among others (Fig. 4c); moreover, the up-regulated genes of SF were particularly associated with “biosynthesis of amino acids,” “phenylpropanoid biosynthesis,” “phenylalanine metabolism,” and “flavonoid biosynthesis,” among others, at DAF45 (Fig. 4d).
Common expression patterns were employed to further analyze the DEGs between SL vs. UL and SF vs. UF at three stages; overall, 7244 (leaf) and 1707 (fruit) DEGs were placed into four clusters (Fig. 5). Most of the candidate DEGs was categorized into either leaf Cluster 1 (3796 genes) or fruit Cluster 1 (1271 genes). By KEGG pathway enrichment, the biological pathway of enrichment in each similar regulatory transcription cluster was described. The DEGs in leaf Cluster 1 exhibited peak expression at DAF45 of SL, including a broad range of genes responsible for porphyrin and cholorophyll metabolism, circadian rhythm-plant, photosynthesis-antenna proteins, carotenoid biosynthesis, peroxisome, and carbon fixation in photosynthetic organisms. The DEGs were mainly involved in photosynthesis, which reflected that leaves can maintain an effective adaptability from DAF35 to DAF45 after a long duration of rain-sheltered covering. For the top five accumulated pathways in leaf Cluster 2, the DEGs showed peak expression at DAF45 of UL, including peroxisome, phenylpropanoid biosynthesis and glutathione metabolism, and these DEGs were mainly involved in the antioxidant system (Fig. 5a). Meanwhile, for the top four enriched pathways in fruit Cluster 1, the DEGs showed peak expression at DAF35 of SF, including a broad range of genes responsible for phenylpropanoid biosynthesis, starch and sucrose metabolism, ascorbate and aldarate metabolism, and phenylalanine metabolism. Finally, for the top five enriched pathways in fruit Cluster 2, the DEGs showed peak expression at DAF55 of SF, including flavonoid biosynthesis, plant hormone signal transduction, starch and sucrose metabolism, and phenylpropanoid biosynthesis (Fig. 5b). Transcriptional results indicated that the adaptability of leaves to the microclimate was primarily attributed to the regulation of photosynthetic characteristics, assimilation, antioxidant status, and circadian rhythm. The genes implicated in the biosynthesis of sugars and anthocyanins in the sheltered fruits were up-regulated at DAF35 until DAF55, reflecting the prior accumulation of nutrition compared with that under shelter-free conditions.
To verify the authenticity of the RNA-Seq results, 12 DEGs were randomly selected and their expression profiles were analyzed by qRT-PCR. The results of qRT-PCR analysis showed that the expression profiles of these DEGs were similar to those assessed by RNA-Seq (Fig. 6), confirming the reliability and accuracy of our RNA-Seq data.
Expression of genes involved in photosynthetic system in sheltered leaves
To clarify the molecular adaptability of cherry trees to the microclimatic change during fruit development upon exposure to the shelter covering, genes involved in environmental sensitivity were screened out from the filtered DEGs for further investigation (Figs. 7 and 8). Most of the DEGs encoding antenna proteins, or proteins involved in electron transport, reaction center in photosystem I (PSI) and photosystem II (PSII), and components of CO2 fixation, were highly expressed in SL compared with UL at DAF45 (Fig. 7a).
More than 70 genes were annotated to three metabolic pathways: photosynthesis-antenna proteins (ko00196), photosynthesis (ko00195), and carbon fixation in photosynthetic organisms (ko00710). Therefore, we focused on the transcriptional levels of those genes closely related to the photosynthetic efficiency (Fig. 7a). All annotated DEGs of light-harvesting Chl a/b binding protein complex I and II (LHCs), namely, 17 genes encoding the Chl A/B binding protein complex I (Lcha1, Lcha2, Lcha3, Lcha4, and Lcha5) and 20 genes encoding Chl A/B binding protein complex II (Lchb1, Lchb2, Lchb3, Lchb4, Lchb5, Lchb6, and Lchb7), were up-regulated in SL at DAF45 (Figs. 7a, 8a). Moreover, 13 genes encoding proteins involved in reaction center and electron transport in photosynthesis, including the PSI reaction center subunit X (PsaK), reaction center subunit VI (PsaH), PSI reaction center subunit PsaN (PsaN), PSII oxygen-evolving enhancer protein 2 (PsbP), PSII 10 kDa protein (PsbR), PSII repair protein Psb27-H1 (Psb27), ferredoxin of photosynthetic electron transport (PetF), and H+/Na+-transporting ATPase subunit beta (AtpF), were up-regulated under sheltered covering (Figs. 7a, 8b), whereas one gene of the cytochrome f complex (PetA) and one gene of H+/Na+-transporting ATPase subunit alpha (ATPF1AI) had lower transcription levels. In the carbon fixation pathway, D-ribulose 1,5-bisphosphate (RuBP) and CO2 produced 3-phosphate-glycerate (3-PGA) under the action of ribulose-bisphosphate carboxylase large chain (rcbL) and ribulose-bisphosphate carboxylase small chain (rcbS); then, 3-PGA was reduced to glyceraldehyde-3P (3-PGAld) by glyceraldehyde 3-phosphate dehydrogenase (GADPH) and glyceraldehyde-3-phosphate dehydrogenase (GAPA), which completed the energy storage process of photosynthesis and increased the production and accumulation of photosynthate. Among them, one gene of rbcL, nine genes of rbcS, one gene of GADPH, and seven genes of GAPA were significantly up-regulated under sheltered covering. Notably, the expression of rbcL and GAPA was up-regulated ≥10-fold (Fig. 7a, 8c).
In combination with the PAR-Pn and CO2-Pn curves, the Pn of sheltered leaves was lower, but there were no significant differences between the first two stages of fruit development (Fig. 9). The AQY and ACE of sheltered leaves visibly increased by 13.0 and 23.5%, respectively; meanwhile, the LCP and CCP parameters decreased to 13.87 and 75.62 μmol·m− 2·s− 1 at DAF45 (Additional file 8: Table S7). In general, combined with photosynthetic characteristics and transcriptomic results of leaves, the findings illustrated that sheltered leaves had stronger abilities to capture and utilize the weak light, while maintaining stable CO2 utilization efficiency. This indicated the possibility of good adaptation to the weak light conditions under the sheltered covering in a short time.
Expression of genes encoding photosynthetic pigments in sheltered leaves
The adaptability of plants to weak light is inextricably related to the photosynthetic pigmentation synthesis pathway . In this study, 34 genes involved in porphyrin and chlorophyll metabolism (ko00860) and one gene related to each of terpenoid backbone biosynthesis (ko00900) and carotenoid biosynthesis (ko00906) in sheltered leaves were up-regulated at DAF45 (Fig. 7b). Regarding the DEGs annotated to Chl synthesis and carotenoid biosynthesis, one gene encoding geranylgeranyl diphosphate reductase (CHLP), one gene encoding oxygen-independent coproporphyrinogen III oxidase (hemN), two genes encoding glutamyl-tRNA reductase (hemA), 23 genes encoding magnesium chelatase subunit H (ChlH), and two genes encoding magnesium chelatase subunit I (ChlI), among others, were up-regulated under sheltered covering at DAF45 (Fig. 8d). Additionally, four genes of 15-cis-phytoene synthase (crtB), two genes of 15-cis-phytoene desaturase (PDS), one gene of prolycopene isomerase (crtISO), and two genes of lycopene beta-cyclase (lcyB) were up-regulated under sheltered covering at DAF45 (Fig. 8e). The contents of photosynthetic pigments Chl a, Chl b and Car tended to increase under sheltered covering. Moreover, the contents were higher than those under Cont; for example, Chl a increased by 14 to 16.7%, Chl b by 13.6 to 24%, and Car consistently increased by 20 to 22.6% under sheltered covering (Fig. 10). The transcription levels of genes related to Chl and carotenoid synthesis in the sheltered leaves also fully confirmed that they would not be adversely affected by the sheltered covering (Fig. 7b, 8); conversely, sheltered covering would enhance the gene transcription levels, thereby increasing the pigment content.
Expression of genes involved in antioxidant systems in sheltered leaves
ROS scavenging enzymes such as SOD, catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX), as well as nonenzymatic antioxidants (glutathione, carotenoids, etc.) are essential for ROS detoxification . Malondialdehyde (MDA) can be used as an indicator of lipid peroxidation under different stress conditions. Transcription levels of multiple genes relating to antioxidant capacity including peroxisome (ko04146), phenylpropanoid biosynthesis (ko00940), glutathione metabolism (ko00053), and carotenoid biosynthesis (ko00906), fluctuated slightly with the microclimatic conditions (Fig. 7c). The expression of most antioxidant-related genes was clearly higher in SL than in UL at DAF45; for example, eight genes involved in peroxisome (encoding SOD, and CAT), 32 genes involved in phenylpropanoid biosynthesis [encoding trans-cinnamate 4-monooxygenase, CYP; phenylalanine ammonia-lyase, PAL; 4-coumarate-CoA ligase, 4CL; cinnamyl-alcohol dehydrogenase, CAD; caffeic acid 3-O-methyltransferase, COMT; 5-O-(4-coumaroyl)-D-quinate-3′-monooxygenase, C3’H; caffeoyl-CoA O-methyltransferase, CCoAOMT; and POD]; eight genes involved in glutathione metabolism (encoding glutathione synthetase, GSS; glutathione reductase, GSR; and glutathione S-transferase, GST); and 16 genes involved in carotenoid biosynthesis (encoding zeaxanthin epoxidase, ZEP; violaxanthin de-epoxidase, VED; beta-carotene 3-hydroxylase, CrtZ; beta-ring hydroxylase, LUT5; lycopene beta-cyclase, and lcyB; and abscisic acid 8′-hydroxylase, CYP707A) showed markedly higher expression in SL than in UL at DAF45 (Fig. 7c).
The antioxidant enzyme activities and MDA content in leaves also changed with the change of microclimate associated with sheltered covering. At DAF35, there was no remarkable difference in the activities of SOD, POD, and CAT of SL and UL; however, the MDA content was higher under sheltered covering. Moreover, the activities of antioxidant enzymes were higher than under shelter-free conditions at DAF45, whereas the MDA content was lower than in UL, with the POD, SOD, and CAT activities increasing by 1.7-, 1.9-, and 1.3-fold, respectively (Fig. 11). These results showed that the activities of antioxidant enzymes in leaves increased during RSC, which maintained the ability of plants to scavenge ROS, and prevented the damage of membrane lipid peroxidation to leaves, thus preserving photosynthetic efficiency. The results showed the same trend as the results of gene expression levels.
Expression of genes associated with anthocyanin synthesis in sheltered fruits
The anthocyanin content of mature cherry fruits under sheltered covering increased significantly compared with that of mature cherry fruits under unsheltered covering . The up-regulated genes of fruits were mainly involved in phenylpropanoid biosynthesis (ko00940) and flavonoid biosynthesis (ko00941). The transcriptional levels of more than 25 genes in sheltered fruits began to increase from DAF35 until fruit ripening (Fig. 7d); these included 12 genes involved in phenylpropanoid biosynthesis, with one gene of PAL, six genes of C4H, and five genes of 4CL. Among these, the expressions of C4H and 4CL were up-regulated more than 8.5- and 6.1-fold, respectively. Moreover, for 13 genes involved in flavonoid biosynthesis encoding chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3′-hydroxylase (F3’H), dihydro-flavonol 4-reductase (DFR), and anthocyanidin synthase (ANS), the expressions were remarkably higher in the sheltered fruits at DAF45 (Fig. 12). The results showed that the synthesis of anthocyanin could be accelerated and the accumulation cycle could be prolonged in the sheltered fruits.
Regulation highlighted photosynthetic adaptation under shelter covering
The alteration of the physiological characteristics of leaves/fruits may be involved in adaptive responses to changes in microclimate, and the transcriptional regulation of genes is the molecular basis for plant adaptation to shifts in environmental factors such as light and temperature. Photosynthesis is pivotal for plant growth, yield  as well as fruit quality; therefore, agronomic techniques are generally particularly focused on improving photosynthesis. Under low light intensity, the photosynthetic efficiency of plants mainly depends on the adaptability of light-harvesting apparatus to the microclimatic conditions, in which PSI/PSII represents the adaptability of plants to low light . LHCs in PSI and PSII play important roles in light-harvesting and light protection . Lhcb (PSII) was reported to be transcriptionally amplified under low light intensity to enhance photon capture . The differentially expressed transcripts of plant LHCs in response to low light stress might play collective roles in maintaining the normal metabolic activities under low light intensity; for example, the genes encoding Lchbs from the low-light-tolerant rice cultivar were up-regulated under low light intensity ; in the current study, all the DEGs associated with the members of LHCs, such as Lcha and Lchb, were up-regulated in cherry leaves under shelter covering. A total of 37 genes were involved in the regulation of light capture protein expression (Fig. 7a). Compared with the unsheltered leaves, nine genes encoding Lcha2 and nine genes encoding Lchb1 were up-regulated; their highest expression levels were increased by 6-fold (Lcha2) and 11-fold (Lchb1). In addition, Psb27, which is essential for energy metabolism and effective recovery of photodamaged PSII complex , was also up-regulated in the sheltered leaves. PsbS might stabilize the PSII–LHCII supercomplex structure and could improve electron transmission efficiency ; in the current study, PsbS was up-regulated in the sheltered leaves (Fig. 7a). During the light reaction of photosynthesis, genes encoding LCHs were up-regulated, among which, Lchbs promoted the absorption and transmission of light energy under low light conditions, and the light capture area and light capture efficiency were also improved. In addition, the photosynthetic characteristics of sheltered leaves showed that LCP decreased and AQY increased (Additional file 8: Table S7). It is speculated that these genes play important roles in regulating adaptation leaf adaption to the low light environment associated with the rain-shelter covering, by increasing the efficiency of light absorption and electronic transmission, leading to the improvement of weak light utility.
Rubisco (rbcL and rbcS) is a bifunctional enzyme located in the chloroplast stoma that catalyzes photosynthetic CO2 fixation to form ribulose-1,5-bisphosphate (RuBP) ; the transcriptional levels of rbcL and rbcS genes could directly reflect the photosynthetic efficiency of leaves . Available evidences have justified that the activation status of Rubisco is affected by light intensity ; therefore, the up-regulation of the rbcL and rbcS in cherry leaves under sheltered covering might play a crucial role in CO2 fixation (Fig. 7a). During the CO2 assimilation in photosynthesis, genes encoding Rubisco (rbcL and rbcS) were found to be up-regulated; among them, the gene expression of rbcL increased by 10.8-fold; nine genes of rbcS were also up-regulated (Fig. 8c). Moreover, in terms of the photosynthetic characteristics of sheltered leaves, it was shown that CCP decreased and ACE increased (Additional file 8: Table S7). These results indicated that the enhancement of CO2 assimilation under low light intensity could be ascribed as the up-regulation of the Rubisco-related genes, which play a significant role in the leaf adaptation to the sheltered covering and provide a material basis for vegetative growth and reproductive growth of plants.
Previous studies indicated that the mechanism of Chl synthesis plays a crucial role in adaptation to low-light conditions . Chl synthesis directly affects photosynthetic efficiency , and Chl b is a prerequisite for the stable existence of light-harvesting complex protein (LHCP) . The increase of Chl b was found to be beneficial for increasing LHCP and the absorption of short-wavelength light, thus effectively improved the illumination ability of plants under low light conditions . Furthermore, an obvious increase of Chl b in the low-light-tolerant rice cultivar was beneficial to capture light energy and drive electron transfer under low light, thereby, maintain a high photosynthetic rate [16, 38]. In current case, the the contents (mg·g− 1) of Chl b in sheltered leaves were also significantly increased in comparison to those of the unsheltered (Fig. 9), which was beneficial to the stability of leaf LHCP, thus to enhance the utilization of light energy under weak light. The HemA, HemN, ChlH and ChlL genes play key roles in plant chlorophyll synthesis. Among them, ChlH is a multifunctional protein with roles in plastid-to-nucleus and plant hormone signal transduction pathways. ChlH accelerated the catalytic activity of ChlM, which catalyzes the conversion of Mg-Proto to Mg-protoporphyrin IX monomethyl ester; this is the key enzyme for chlorophyll synthesis, finally resulting in the synthesis of chlorophyll . In the chlorophyll synthesis pathway of sheltered leaves in current research, the expressions of genes encoding HemA, HemN, ChlH, and ChlL were up-regulated in the sheltered leaves. In addition, 23 genes encoding ChlH were up-regulated (Fig. 7b). Therefore, the expression of multiple genes related to Chl synthesis were enhanced under the shelter covering, and the increase of Chl b content improved the LHCP status, leading to the elevation in photosynthetic efficiency of the sheltered leaves (Fig. 10).
Regulation of antioxidant capacity under shelter covering
ROS are produced by plants under stress, which can lead to photoinhibition and photooxidative damage of PSII and PSI . ROS accumulation was shown to be regulated by a series of antioxidant compound; the expression of genes involved in the antioxidant system in plants was found to be diversely inhibited under light stress . The antioxidant enzymes (SOD, CAT, POD, etc.) and nonenzymatic antioxidants (lutein, lignin, glutathione, anthocyanins, etc.) play various roles in the response to abiotic stresses . Under low light conditions, low-light-tolerant rice cultivars were shown to maintain their carbohydrate production level by holding an effective photosynthetic rate and oxygen resistance . Similarly, the activities of antioxidant enzymes in sheltered leaves were higher, which could avoid membrane lipid peroxidation and help maintain photosynthetic efficiency (Fig. 11). Meanwhile, one gene for SOD, two genes for CAT, and six genes for POD were found to be up-regulated, whereas five genes were inhibited in the leaves from the sheltered covering (Fig. 7c). Therefore, the improvement of photosynthetic capacity of sheltered leaves might be considerably contributed to ROS scavenging.
Carotenoids may capture light energy, also, it play a key role in light protection; β-carotene participates in the energy-transfer process and plays an essential role in scavenging singlet oxygen in the photosynthetic reaction centers . In the carotenoid synthesis pathway, nine genes encoding crtB, PDS, crtISO, and lcyB were up-regulated in rain-sheltered leaves (Fig. 8d), and the level of carotene was higher in the sheltered leaves (Fig. 10). Furthermore, lutein has the photoprotective function of quenching triplet Chl and excited singlet Chl in light-harvesting antenna systems , which provides antioxidant activity by scavenging oxides, thus preventing lipid peroxidation . In the current study, five genes for LUT5, crtZ, and lcyB that synthesize lutein were up-regulated in sheltered leaves. These results further proved that the sheltered leaves enhanced the genes expression of carotenoid synthesis, and somehow promoted photosynthesis.
It is reported that the genes encoding PAL, 4CL, CYP and CCoAOMT in phenylpropanoid pathway in shade tolerant soybean cultivar were up-regulated in low light environment . Similarly, 14 genes for PAL, 4CL, and CYP were found to be up-regulated under the sheltered leaves herein (Fig. 7c). Glutathione (GSH) can be used as an electron donor to inactivate free radicals, or as a cofactor of several antioxidant enzymes  for the detoxification of hydrogen peroxide produced under different stress conditions ; in this study, genes for GSS and GSR were also up-regulated in cherry leaves under the sheltered covering (Fig. 7c).
Based on the above-mentioned results, the antioxidant capacity of sheltered leaves was integrated with the antioxidant enzymes and a variety of nonenzymatic antioxidants to maximize the protection effect, which reduce the negative effect of low light intensity, somewhat maintaining higher photosynthetic efficiency of the sheltered leaves.
Regulation of anthocyanin biosynthesis under shelter covering
Solar radiation, air humidity, and air temperature in the sheltered covering were reported to be affected by a rain-shelter covering . Anthocyanin accumulation was also influenced by microenvironmental changes . Meanwhile, the light quality could affect the synthesis of anthocyanin by regulating the expression patterns of related genes . Therefore, anthocyanin content is governed by the differential expression of corresponding genes in a metabolic network, which was ascribed to the change in environmental factors . Available evidence shows that VvPAL plays a key role in the phenylpropane biosynthesis pathway (pathway upstream of anthocyanin synthesis) by catalyzing phenylalanine to cinnamic acid . Structural genes involved in anthocyanin biosynthesis in Arabidopsis thaliana shown to be enzymes expressed in the initial and late stages of the regulation of the flavonoid biosynthesis pathway (pathway downstream of anthocyanin synthesis), such as CHS and CHI (initial stage), and DFR and ANS (late stage) are essential for anthocyanin biosynthesis . In addition, CHS catalyzes the initial steps of the phenylpropanoid pathway branching to the flavonoid pathway, which is particularly important for the synthesis and accumulation of fruit anthocyanin . In sweet cherry, DEGs including PAL, 4CL, CHS, CHI, F3’H, DFR, and ANS were confirmed to be involved in anthocyanin synthesis . With the progression of cherry fruit ripening, the genes involved in anthocyanin synthesis were presumably up-regulated under unsheltered conditions . In another study, the levels of phenolic compounds in grape peel under shelter covering were found to be decreased in comparison with those under shelter-free conditions . In addition, a previous study, it was proven that, in grapes under the sheltered covering, the transcript abundance of VvF3’5’H and VvF3’H was significantly reduced , which contrasts with the results of the current study. In this study, these DEGs involved in phenylpropanoid and flavonoid biosynthesis, such as PAL, C4H, 4CL, CHS, and CHI, were up-regulated under covered conditions (Fig. 7d). Among these, the expression of three genes encoding 4CL increased over 3.5-fold and that of one gene encoding C4H increased 8.5-fold. In the flavonoid biosynthesis pathway, compared with the case for fruit grown under shelter-free conditions (DAF45), DEGs encoding CHS, CHI, F3’H, DFR, and ANS were up-regulated, of which the expression levels of five genes encoding CHS increased more than 3.5-fold (Fig. 12). Strong evidence showed that, compared with the unsheltered sweet cherry, the average level of phenols in covered sweet cherry increased by 14%. The results demonstrated that phenolics present under covered conditions can be explained by the larger temperature fluctuations and moderate but not excessive heat stress under the lower-light conditions under the cover . Previous research also showed that anthocyanin synthesis was restricted by upstream metabolites (such as sugar) during fruit development, which proved that anthocyanin accumulation is closely related to photo-assimilates . Furthermore, the results for grapes proved that the increase in anthocyanin content was attributable to a prolonged life of functional leaves, an increase in assimilative products of leaves, and an increase in the soluble solid content of fruits under sheltered covering; ultimately, the content of anthocyanin was also increased . From the results of transcriptomic regulation in the current study, anthocyanin synthesis of fruit under sheltered covering increased the anthocyanin content of fruit by synergizing with key enzymes in upstream/downstream pathways. The synthesis and accumulation of anthocyanins could be increased by increasing the upregulation of genes involved in phenylpropane biosynthesis and flavonoid biosynthesis. Of course, this process was also inseparable from the adaptation of leaves to weak light, which provided assimilation products for the synthesis of fruit anthocyanins.
Collectively, cherry trees could improve photosynthesis and antioxidant capacity by regulating the genes involved in light capture (LCHs), carbon dioxide fixation (Rubisco), photosynthetic pigment synthesis (ChlH), and the antioxidant system (carotenoids and phenolics), and ultimately enhance their adaptability to low light intensity under sheltered covering.
In summary, we produced some new transcriptome information from Chinese cherry with shelter covering using PacBio and Illumina sequencing in cherry leaves and fruits at three developmental stages under control and rain-shelter covering. The adaptive regulation of cherry leaves and fruits under the sheltered covering was mainly associated with their physiological characteristics (such as LCP, CCP, and Chl content) and transcriptome expression profiles. Also, the transcriptome data confirmed that the adaptation mechanism of leaves to low light was mainly achieved by regulating the gene transcription profiles of LHCP synthesis, photosynthetic electron transfer, CO2 fixation, as well as photosynthetic pigment and antioxidant synthesis. Moreover, the sheltered leaves maintained a higher ability for light capture under low light intensity by actively up-regulating Lcha and Lchb expression, promoted CO2 fixation by elevating the expressions of rbcL and rbcS, and enhanced the expression of chlH, thereby improved Chl synthesis. Additionally, improvement in the antioxidants biosynthesis of carotenoid, phenylpropanoid, and glutathione could comprehensively ensure leaves produce enough carbohydrates under low light intensity. Finally, the genes related to anthocyanin biosynthesis, e.g. PAL, C4H, 4CL, CHS, CHI, F3’H, DFR, and ANS were significantly up-regulated in the fruits under the sheltered covering, which might contributed to the anthocyanin accumulation in sheltered fruits. The present study and its findings can serve as valuable resources for future genomic studies on Chinese cherries. The DEG data may also provide useful candidate genes to elucidate the adaptation mechanism of Chinese cherries and other varieties to low-light condition and other abiotic stress.
The trials were conducted in 2016–2018 in an orchard located at Fuquan, Guizhou Province, P.R. China (latitude 26°70′ N, longitude 107°51′ E), which is characterized by a subtropical monsoon climate. At the site, the average temperature is 14 °C, the relative humidity is 88%, and the annual total precipitation is 1220 mm. Five-year-old Chinese cherry trees (“Manaohong” cultivar) were spaced at 3 m between each row and each tree, with the open-center model of trunks and branches. All the trees were subjected to management using the same integrated technique. The trials were conducted with by covering with colorless polyethylene film (semi-covered to ensure ventilation), with a transmission rate of approximately 70%, which was applied from before blooming until fruit harvest. The length, width, and height of the steel frame shelter were 30 m, 10 m, and 4 m (above the ground), respectively. Unsheltered trees were set as a control (Cont). Three trees each under sheltered and unsheltered conditions were labeled for sample collection on the 35th, 45th, and 55th days after flowering (DAF35, DAF45, and DAF55, respectively). Samples of unsheltered leaves (UL35, 45, and 55), unsheltered fruits (UF35, 45, and 55), sheltered leaves (SL35, 45, and 55), and sheltered fruits (SF35, 45, and 55) were collected; immediately frozen in liquid nitrogen; and stored at − 80 °C for physiological indicator measurement and RNA isolation.
Physiological characteristics of leaves under rain-shelter covering
The photosynthesis light-response (PAR-Pn) and CO2-response (CO2-Pn) curves under shelter covering and shelter-free conditions were measured between 9:00 a.m. and 11:30 a.m. on a sunny day using a portable infrared gas analyzer (Li-6400XT; Li-Cor, Inc., Lincoln, NE, USA). The photosynthetic PAR-response and CO2-response curves were fitted by the Farquhar mathematical model (Prioul and Chartier, 1977). The AQY and CE were obtained by linear regression under a PAR range of 0–200 μmol·m− 1·s− 1 and CO2 concentration range of 0–200 μmol·mol− 1; in addition, the LCP and CCP were calculated. Two new southern branches were selected for each tree (three trees per sheltered covering), and the fifth mature leaf was selected from the base of the shoot.
Thirty mature leaves per tree were sampled to quantify the following parameters, and three biological replicates were performed for each of the sheltered and unsheltered conditions. To minimize the positional effect of the canopy, leaves were harvested from four directions: east, south, west, and north. The contents of Chl a, Chl b, and carotenoids (Car) were determined according to the method described by Shi et al. (2013). The amount of malondialdehyde (MDA) and activity of superoxide dismutase (SOD, E.C.188.8.131.52) were measured as documented by Wen et al. (2011). The activities of peroxidase (POD, E.C. 184.108.40.206) and catalase (CAT, E.C. 220.127.116.11) were determined using a spectrophotometer following a previously described method (Sharma et al., 2016). All absorbances of extracts were measured using a microplate spectrophotometer (Thermo Scientific, MA, USA).
Total RNA extraction, PacBio and Illumina library construction and sequencing
In total, 36 samples [two sheltered coverings (sheltered and unsheltered) × three stages (DAF35, 45, and 55) × two tissues (leaves and fruits) × three biological replicates] were prepared, and the total RNAs were separately obtained using the QIAGEN RNeasy Plus Mini Kit (Cat. No. 74134). The purity and concentration of RNA were determined using a Nanodrop 2000 spectrophotometer and QUBIT Fluorometer (Life Technologies), and the integrity of RNA was assessed using an Agilent 2100 bioanalyzer (Agilent Technologies).
The qualified RNAs of leaves (UL and SL) and fruits (UF and SF) from three stages (DAF35, 45, and 55) were equivalently mixed into two corresponding libraries. cDNA was synthesized using a SMART PCR cDNA Synthesis Kit (Clontech, Cat. No. 639206). PCR was optimized using a KAPA HiFi PCR Kit; different cDNA fractions (0.5–2 kb, 2–3 kb, and > 3 kb) were classified using the BluePippin size selection system (Sage Science, Beverly, MA, USA). The PCR products were amplified and constructed using SMRTbell Template Prep Kit 1.0 (part.100–259-100) following the vendor’s protocol. The library was sequenced on the PacBio Sequel real-time nanopore sequencer using PacBio Sequel V2.1 with five cells (one cell for fruits and four cells for leaves); 0.5–6 kb mixed libraries were sequenced. In total, 36 qualified RNA samples were used for Illumina sequencing. The RNA samples were used for poly(A)+ selection using oligo (dT) magnetic beads, while constructing RNA-Seq libraries. The cDNA libraries were sequenced using PacBio Sequel and HiSeq X Ten at Wuhan Nextomics Biosciences Co., Ltd.
Data processing and gene function annotation
Sequence data of PacBio Sequel were collected, and the high-quality consensus transcript sequences were obtained using smrtlink 5.0. Subsequently, the iterative clustering for error correction (ICE) algorithm was used to remove redundancy and improve the accuracy of the full-length transcripts. Raw data of Illumina HiSeq were processed through inner Perl scripts. Clean data were obtained by removing reads containing adapters, reads containing poly-N regions, and low-quality reads from the raw data. Meanwhile, the Illumina HiSiq reads were used to correct all full-length transcripts of single-molecule real-time (SMRT) Squel by Lordec . The proofread-corrected sequences after removal of the redundant sequences using CD-HIT-EST  were used as reference sequences for further analyses. Gene functions were searched against the databases of NR, COG, Swiss-Prot, KEGG, and GO.
Differential expression, GO, and KEGG enrichment analysis
Clean reads of all samples were counted and normalized into fragments per kilobase of transcript per million fragments mapped reads (FPKM) value using RSEM (v1.1.12) . DEGs between different treatments were determined using the DESeq package . The FDR values were < 0.05 and an absolute value of log2FoldChange ≥1 (or < − 1) were used to identify genes that were significantly differentially expressed. The GO functional classification statistics of DEGs involved the use of the WEGO software . Metabolic pathway assignments of DEGs were implemented based on the KEGG Orthology database (http://www.genome.ad.jp/kegg/) using the KAAS system (http://www.genome.jp/tools/kaas/). The overall pathway analyses were mainly based on the tool Mercator4 and Mapman4 . MapMan BIN was inferred based on both automatic and manual annotations. The different expression patterns of DEGs among the leaves and fruits were assessed using the R language, Cluster, Fpc, Ggplot2, and Reshape2 packages; the clustering method was K-means clustering.
Quantitative real-time PCR (qRT-PCR) analysis
The expression levels of samples under the different conditions, including leaves and fruits, were validated by qRT-PCR using PowerUp™ SYBR Green Master Mix (ThermoFisher, Chongqing, China) in a volume of 10 μL, which contained 5 μl of SYBR Green Master Mix, 150 ng (leaf)/100 ng (fruit) of cDNA template, and 0.4 μM of each of the forward and reverse primers. The qRT-PCR amplification was performed as follows: 95 °C for 30 s, followed by 40 cycles of 95 °C for 5 s and 60 °C for 30 s. Three cherry actin genes (UBCE, CYP2, and ACT2) were used as reference genes, as previously reported [64, 65]; the primer sequences are listed in Table S2. Relative gene expression was calculated using the 2−ΔΔCt method  with the CFX Connect™ Real-Time PCR Detection System (Bio-Rad Laboratories, CA, USA). All validations were performed in three biological and technical replicates.
All data were assessed for significant differences using Duncan’s and Tukey’s tests using the SPSS 21.0 statistics package (Chicago, IL, USA). All data are presented as the mean and standard deviation (SD) of at least three replicates. The graphs were constructed with Origin 9.0 (Origin Lab, Northampton, MA, USA).
Availability of data and materials
Circular consistent sequence
Differentially expressed genes
Iterative clustering for error
Kyoto Encyclopedia of Genes and Genomics
Light-collecting complex protein
Photosynthetically active radiation
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The project was supported by grants from the National Natural Science Foundation of China (31760552), Core Program of Guizhou Province, P. R. China (2016–2520), the Innovation Talent Program of Guizhou Province, P. R. China (2016–4010), as well as the Construction Program of Biology First-class Discipline in Guizhou (GNYL  009). We thank all the foundation of economic support. The funding organizations provided the financial support to the research projects, but were not involved in the design of the study, data collection, analysis of the data, or the writing of the manuscript.
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The experiments did not utilize transgenic technology or involve protected species. “Manaohong” used in this study were obtained from an orchard in Fuquan, which was a demonstration base of Guizhou University, China. It does not require specific permits.
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Additional file 2: Table S1. Summary of PacBio Sequel real-time sequencing. The four rows of “SL and UL1-4” represent the leaf library, and the row “SF and UF” represent the fruit library. aPolymerase, the original read generated by PacBio Sequel; bSubreads, Post-filter polymerase reads; cSequencing times of insert; dNumber of circular consensus sequences; eNumber of full-length non-chimeric.
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Tian, T., Qiao, G., Wen, Z. et al. Comparative transcriptome analysis reveals the molecular regulation underlying the adaptive mechanism of cherry (Cerasus pseudocerasus Lindl.) to shelter covering. BMC Plant Biol 20, 27 (2020). https://doi.org/10.1186/s12870-019-2224-x
- Rain shelter
- Transcriptional regulation
- Anthocyanin biosynthesis