Fig. 4

Expression of candidate genes with significant root specificity and low root preference. The expression of 18 genes (black borders) highly expressed in root at three stages was analyzed by qRT-PCR. Two candidate genes (blue borders) with low root preference are also shown. The maize Actin1 gene was used as an internal control, and leaf was used as a reference sample. For qRT-PCR, three biological replicates were used, each with four technical replicates. Error bars indicate standard deviations