Table 1 Statistical results of the SMRT sequencing data
cDNA size | 1–2 kb | 2–3 kb | >  3 kb | All |
|---|---|---|---|---|
SMRT cells | 3 | 3 | 2 | 8 |
Polymerase reads | 450,876 | 450,876 | 300,584 | 1,202,336 |
Postfilter number of subreads | 4,761,465 | 2,481,575 | 1,185,345 | 8,428,385 |
Reads of insert | 240,441 | 189,779 | 122,598 | 552,818 |
Number of 5′ reads | 122,516 | 120,763 | 84,001 | 327,280 |
Number of 3′ reads | 139,768 | 126,512 | 87,086 | 353,366 |
Number of poly-A reads | 133,709 | 124,866 | 86,112 | 344,687 |
Number of filtered short reads | 42,363 | 11,932 | 2853 | 57,148 |
Number of non-full-length reads | 100,142 | 75,634 | 47,543 | 223,319 |
Number of full-length reads | 97,936 | 102,213 | 72,202 | 272,351 |
Number of full-length nonchimeric reads | 97,156 | 101,959 | 71,635 | 270,750 |
Average full-length nonchimeric read length | 1309 | 2282 | 3536 | 2264 |
Full-length percentage (FL%) | 40.73% | 53.86% | 58.89% | 49.27% |
Artificial concatemers (%) | 0.80% | 0.25% | 0.79% | 0.59% |