Fig. 8

MC regulates the expression of cell cycle and cell division related genes. a Expression dynamics of genes differentially expressed at all three time points. DEGs satisfy |log₂Fc| ≥ 1 at least one time point were collected. K1:down-down; K2: up-down; K3:up-up. Values in boxes are log₂Fc (show |log₂Fc| ≥ 1 only). b MC overall induces genes in cell cycle and cell division. White digit indicated the gene number. The percentage represent the ratio of up−/down-regulated genes number to the total number of regulated-genes. Cotton seeds of K638 were treated with deionised water (Control) or 400 mg/L MC for 12 h. The roots sampled at 12, 48, and 72 h after treatment were subjected to RNA-seq analyses. “R” indicates the whole root, “M” indicates the root middle region (4 to 20–40 mm from the root tip), “T” indicates the root tip region (0 to 4 mm from the root tip). 3 biological replicates per treatment, 30 roots per replicate