Fig. 4
From: Roles of the SPL gene family and miR156 in the salt stress responses of tamarisk (Tamarix chinensis)
Identification and validation of miR156-targeted SPLs of Tamarix chinensis (TcSPLs). a Graphic representation of the cDNAs of all TcSPLs carrying the predicted MRE (red and blue boxes). Coding sequences are shaded grey, and the conserved SBP-box is shaded with diagonal. b Alignment of the predicted MRE sequences compared with the miR156 reverse complementary sequence (TcmiR156). Nucleotide divergences compared with TcmiR156 are highlighted by colours. Reading frames are indicated by triplet codons, and the target motif is represented by amino acid residues. c Sequence logos of MRE within TcSPLs and putative SPL orthologues of Arabidopsis thaliana and Populus trichocarpa (AtSPLs and PtSPLs). TcSPL1/3/8-MRE was aligned with, TcSPL2/4-MRE was aligned with, TcSPL1/3/8-MRE was aligned with, and the TcSPL5i-MRE targeted motif was aligned with. d Schematic representation of the dual-luciferase reporter (DLR) assay. The reporter and effector were transferred into Populus protoplasts after overnight transformation. The ratio of firefly luciferase to Renilla luciferase of four MREs represents the activity of the miR156-TcSPL interaction. mMRE are MRE mutated in 10-11th nucleotides of TcmiR156. Data are represented by percentage with SEs (n = 4). Significant differences were determined by Student’s t-test (**P < 0.01, *** P < 0.001)