Fig. 6

ALA induced GSTU43 expression and GST activity in partial GSTU43-silenced plants exposed to low temperature. a GSTU43 expression. The gene transcription levels in pTRV plants under normal condition at 24 h was normalized as 1, (b) GST activity. The VIGS plants leaves were treated with distilled water or 25 mg·L^− 1 ALA, then exposed to normal condition (22 °C/20 °C, day/night) or low temperature (15 °C/8 °C, day/night) 12 h later. After 24 h of low temperature, these indexes were measured. Data are expressed as the mean ± standard error of three independent biological replicates. The experiments were repeated for three times. Different letters above the bars indicate a significant difference determined by one-way ANOVA with Tukey’s test (P < 0.05)