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Table 1 Top 10 residues likely to display interaction between Twinkle protein and Pol1A or Pol1B as predicted by Direct Coupling Analysis (DCA). Amino acid designations and tri-alanine mutants for each of these residues is also displayed

From: Arabidopsis thaliana organelles mimic the T7 phage DNA replisome with specific interactions between Twinkle protein and DNA polymerases Pol1A and Pol1B

Twinkle
Residue number Amino acid Tri-Alanine Mutant created
 124 Glu ∆ 123,124,125
 126 Cys ∆ 126,127,128
 127 Pro
 159 Leu ∆ 158,159,160
 177 Ala ∆ 176,177,178
 185 Asp ∆ 184,185,186
 189 Lys ∆ 188,189,190
 233 Gly ∆ 232,233,234
 430 Thr ∆ 429,430,431
 431 His
Pol1A/B
Residue number Amino acid (Pol1A/Pol1B) Tri-Alanine Mutant created*
 118 Val/Glu ∆ 117,118,119
 248 Arg/Asp ∆ 247,248,249
 255 Val/Glu ∆ 254,255,256
 412 Pro/Lys ∆ 411,412,413
 422 Glu/Asp ∆ 421,422,423
 427 Leu/Lys ∆ 426,427,428
 495 His/Phe ∆ 494,495,496
 591 Val/Lys ∆ 590,591,592
 634 Asn/Val ∆ 633,634,635
 695 Thr/Gly ∆ 694,695,696
  1. *Mutants were created for Pol1A and not Pol1B