BMC Plant Biology

Table 1 Top 10 residues likely to display interaction between Twinkle protein and Pol1A or Pol1B as predicted by Direct Coupling Analysis (DCA). Amino acid designations and tri-alanine mutants for each of these residues is also displayed

From: Arabidopsis thaliana organelles mimic the T7 phage DNA replisome with specific interactions between Twinkle protein and DNA polymerases Pol1A and Pol1B

Twinkle
Residue number	Amino acid	Tri-Alanine Mutant created
124	Glu	∆ 123,124,125
126	Cys	∆ 126,127,128
127	Pro	∆ 126,127,128
159	Leu	∆ 158,159,160
177	Ala	∆ 176,177,178
185	Asp	∆ 184,185,186
189	Lys	∆ 188,189,190
233	Gly	∆ 232,233,234
430	Thr	∆ 429,430,431
431	His	∆ 429,430,431
Pol1A/B
Residue number	Amino acid (Pol1A/Pol1B)	Tri-Alanine Mutant created*
118	Val/Glu	∆ 117,118,119
248	Arg/Asp	∆ 247,248,249
255	Val/Glu	∆ 254,255,256
412	Pro/Lys	∆ 411,412,413
422	Glu/Asp	∆ 421,422,423
427	Leu/Lys	∆ 426,427,428
495	His/Phe	∆ 494,495,496
591	Val/Lys	∆ 590,591,592
634	Asn/Val	∆ 633,634,635
695	Thr/Gly	∆ 694,695,696

*Mutants were created for Pol1A and not Pol1B

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ISSN: 1471-2229

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