Fig. 4
![Fig. 4](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12870-019-1854-3/MediaObjects/12870_2019_1854_Fig4_HTML.png)
Regions of Pol1A, Pol1B, and Twinkle involved in the highest number of yeast-two-hybrid interactions. The regions highlighted above are still rather large and were selected for further truncation to specify exactly which regions of the protein led to positive interaction