Fig. 2From: Generation of a new thermo-sensitive genic male sterile rice line by targeted mutagenesis of TMS5 gene through CRISPR/Cas9 systemPCR-based identification of T-DNA-free T1 segregants using primers directed at the hpt sequence. Segregants among (a) tms5–1 plants, and (b) tms5–2 plants. VK005–01 was used as the positive control (CK+) and cv. YK17 as the negative control (CK-). M: 2 kbp DNA ladderBack to article page