Fig. 2

Growth of transgenic and WT seedlings. Seven-day-old WT and transgenic seedlings were transferred from MS plates into soil (4 plants/pot) grown for 4 weeks. The plants were then treated with 0 or 200 mM NaCl. Ten days post-treatment, the plants were photographed (a) and their fresh weight was measured (b). The two preparations were performed at different times to more accurately assess the salt tolerance of the transgenic plants and (c) the relative change (percentage reduction) in fresh weight in the presence of salt stress relative to the nonstressed control was determined. Data are presented as mean ± SE of nine replicates. Different letters above the columns indicate statistically significant differences at a p < 0.05 level among the different experimental cohorts. SpSOS1, SpSOS1-overexpressing plants; SpAHA1, SpAHA1-overexpressing plants; SpSOS1 + SpAHA1, SpSOS1 and SpAHA1 co-expressing plants; WT, wild-type plants