- Research article
- Open Access
Natural variation in a CENTRORADIALIS homolog contributed to cluster fruiting and early maturity in cotton
BMC Plant Biology volume 18, Article number: 286 (2018)
Plant architecture and the vegetative-reproductive transition have major impacts on the agronomic success of crop plants, but genetic mechanisms underlying these traits in cotton (Gossypium spp.) have not been identified.
We identify four natural mutations in GoCEN-Dt associated with cluster fruiting (cl) and early maturity. The situ hybridization shows that GhCEN is preferentially expressed in cotton shoot apical meristems (SAM) of the main stem and axillary buds. Constitutive GhCEN-Dt overexpression suppresses the transition of the cotton vegetative apex to a reproductive shoot. Silencing GoCEN leads to early flowering and determinate growth, and in tetraploids causes the main stem to terminate in a floral bud, a novel phenotype that exemplifies co-adaptation of polyploid subgenomes and suggests new research and/or crop improvement approaches. Natural cl variations are enriched in cottons adapted to high latitudes with short frost-free periods, indicating that mutants of GoCEN have been strongly selected for early maturity.
We show that the cotton gene GoCEN-Dt, a homolog of Antirrhinum CENTRORADIALIS, is responsible for determinate growth habit and cluster fruiting. Insight into the genetic control of branch and flower differentiation offers new approaches to develop early maturing cultivars of cotton and other crops with plant architecture appropriate for mechanical harvesting.
Cotton (Gossypium spp.) is the world’s most important natural fiber crop, a significant oilseed, and an important source of high-quality protein . The Gossypium genus includes 45 diploid and 7 tetraploid species . Two allotetraploids, G. hirsutum L. and G. barbadense (2n = 4x = 52, AADD), originating from trans-oceanic dispersal of an A-genome African species, G. herbaceum (A1) or G. arboreum (A2) (2n = 2x = 26, AA) and hybridization with an American D-genome species, G. raimondii (2n = 2x = 26, DD), respectively provide 95 and 2% of worldwide cotton production  from cultivation in about 80 countries .
Improved productivity to meet increased consumption of cotton fiber has heavily relied upon mechanization of traditionally labor-intensive tasks . As was true in rice and maize [5, 6], genetic modification of plant architecture is of great importance to optimizing cotton for mechanized production . A variety of architectures have been proposed and used to adapt to polytropic climates and to improve cotton yield potential in breeding programs .
Cotton has a complex growth pattern due to perennation, indeterminate growth, and sympodial fruiting of its wild ancestors [9, 10]. Indeterminate growth affects the distribution of reproductive structures, node number and yield. Determinate growth habit is conferred by a single recessive gene associated with the ‘cluster’ (cl) trait, of central importance for mechanical harvest [11, 12]. Now the world’s largest cotton producer, the introduction into China of the two tetraploid cultivated species in the last century included G. barbadense genotypes with the cl trait, which was incorporated into many cultivars in Xinjiang Province. Mutants of the more widely grown G. hirsutum L. have also been identified and used in breeding programs.
In the present study, we characterized a cl mutant with determinate cluster fruiting. Using map-based cloning, we identified the GoCEN gene corresponding to the cl trait. Transgenic evidence, transcriptome and polymorphism analysis show that natural mutation in GoCEN has facilitated cotton architecture and early maturation throughout a long history of scientific cotton breeding.
The cl trait affects the distribution of reproductive structure
The cl trait is a crucial factor controlling plant architecture and shows wide genetic variations in G. hirsutum and G. barbadense. The wild-type growth habit is ‘indeterminate’, with continuous production of fruiting branches, and the cl mutant is ‘determinate’ with clustered fruit (Additional file 1: Figure S1). To quantify the impact of cl on cotton architecture, we compared the first fruiting branches of three Upland (G. hirsutum wild type CCRI35; cl mutants Duan063 and Chaozao3) and three Sea-Island genotypes (G. barbadense wild type Pima S-6; cl mutants Hai170 and Xinhai18). The cl mutants were characterized by fruiting branch termination, and reduced numbers of nodes, leaves and bolls (Fig. 1), showing that cl contributes substantially to variations of plant architecture and reproductive structure.
Map-based cloning of cl
To understand the hereditary basis of the cl trait, an F2 population including 310 progenies was constructed by crossing CCRI 35 with Hai170. The segregation ratio of normal (238) and cluster fruiting plants (72) did not deviate significantly from 3:1 (χ2 = 0.572 < χ20.05 = 3.84), indicating that the Gb-cl trait was controlled by a single recessive gene. At least three cl genes are known in cotton cl1 and cl3 on chromosome 16 (D07), and cl2 on chromosome 7 (A07) . Using 100 SSR markers on chromosome 07 and chromosome 16 from an interspecific genetic map , Gb-cl was mapped to a 600-kb region including 20 genes on chromosome 16 , delimited by markers CIR100 and STV023 (Fig. 2a and Additional file 1: Figure S2a). Another 559 SSR markers developed based on the G. raimondii genome  were used to screen CCRI 35 and Hai170, finding 82 markers on chromosome 16 and linked to Gb-cl (Additional file 1: Figure S2b and Additional file 2: Date S1). Using another 2341 F2 plants, Gb-cl co-segregated with five SSR marker was narrowed to a 0.2 cM region between markers SWU07707 and SWU08487 (Fig. 2c and Additional file 1: Figure S2c), corresponding to a 69-kb region on chromosome 01 of G. raimondii  and a 139.4 kb-region on D07 of G. hirsutum , respectively (Fig. 2d and e). Only one or two genes were annotated in this region in G. raimondii (Gorai.001G121800) or G. hirsutum (GohirD07G113500 and Gohir.D07G113600), and Gorai.001G121800 and GohirD07G113500 encoded a TFL1-like/CEN-like gene. In a second F2 population with 184 individuals from a cross between Duan063 and Pima-S6, Gh-cl was co-segregated with two SSR markers, SWU10320 and SWU7712, which also co-segregated with Gb-cl (Additional file 1: Figure S2c). In a third large F2 population with 2236 individuals from a cross between Yumian1 and Chaozao3, Gh-cl was narrowed to a 0.8-Mb region flanked by SWU7649 and SWU7554, containing thirty-six putative open reading frames (Additional file 1: Table S1) including GohirD07G113500.
Phylogenetic investigation indicated that GohirD07G113500 is a co-ortholog of CENTRORADIALIS (CEN) in Antirrhinum and SELF-PRUNING (SP) in tomato (Additional file 1: Figure S4), which regulate inflorescence architecture and flowering time, respectively [11, 17]. Based on these results, we focused on GohirD07G113500 (GhCEN-Dt) as a candidate for the cl trait.
To clarify sequence variations of cl, we cloned the corresponding genomic sequences from five G. hirsutum cultivars/lines (two wild-type, Yumian1 and CCRI 35; three cl mutants, Chaozao3, Duan063 and Xiaoxian2) and four G. barbadense cultivars/lines (two wild-type, Pima S-6 and 3–79; two cl mutants, Hai170 and Xinhai18). Based on the CL genomic sequence from G. hirsutum TM-1 , we found 3 mutations differentiating G. hirsutum genotypes from CCRI35 and Yumian1. In Chaozao3, an ‘A’ to ‘G’ nucleotide substitution changed amino acid Asp (D) to Asn (N); in Duan063, a single ‘A’ nucleotide acid was deleted between 1035 and 1037 bp; in Xiaoxian2, two nucleotides, ‘CA’, were deleted between 387 and 390 bp (Fig. 2f and Additional file 1: Figure S5). In G. barbadense, Hai170 and Xinhai18 had the same mutation: a ‘T’ to ‘C’ substitution changed proline (P) to Ser (S). Analysis of CEN cDNA sequences confirmed two prominent polymorphisms between G. hirsutum and G. barbadense (Additional file 1: Figure S5 and Table S2), also showing that four SNPs differentiate the At_genome and Dt_genome alloalleles.
Spatiotemporal expression pattern of GhCEN
Four SNPs differentiated homoeologous GhCEN-At and GhCEN-Dt transcripts (Additional file 1: Figure S5 and Table S2), but the RT-PCR primer designed to amplify GoCEN could not distinguish between GhCEN-At and GhCEN-Dt. Real-time PCR revealed that GhCEN expression was much higher in cotton main stem and fruit branch apices than in root, stem, leaf, pistil, petal, sepal, ovule or embryo (Fig. 3a). There were no significant differences in GoCEN expression between wild-type and mutant cottons from both G. barbadense and G. hirsutum, respectively, except Xiaoxian2 for which a two base deletion results in an open reading frame shift mutation (Fig. 2f).
The situ hybridization (Fig. 3c) revealed GoCEN to be expressed throughout all organ primordia. GoCEN is preferentially expressed in the axillary and apical meristems of the main stem, specifically in the narrow domain around the provascular bundles and in the growing tips (Fig. 3c). These results support the hypothesis that GoCEN plays a crucial role in the development of all axillary buds.
To determine the subcellular localization of GhCEN, two vectors 35S::GhCEN-Dt-GFP and 35S::GhCEN-At-GFP were transformed into Nicotiana benthamiana. Fluorescence detection result showed that both GhCEN-Dt-GFP and GhCEN-At-GFP were located in the nucleus of transformed tobacco plants (Fig. 3d).
GhCET-Dt over-expression delays the cotton vegetative to reproductive transition
To determine the function of the GbCEN-Dt mutation, two overexpression vectors containing full-length coding regions driven by 35S promoters, named 35S::CEN (from CCRI 35) and 35S::cen (from Hai170), were constructed and transformed into Jimian 14 (an indeterminate Upland cotton). Totals of 6 and 9 independent transgenic T0 lines were obtained for 35S::CEN and 35S::cen, respectively (Fig. 4a-c). Three 35S::CEN and three 35S::cen T0 lines with high expression were selected for further analysis (Fig. 4e). The first fruiting branch of wild-type plants usually occurs on the sixth or seventh node of the main-stem, whereas in 35S::CEN plants it occurred on the twelfth node (Fig. 4f). As showed in Fig. 4 g and h, at the same growth stage, 35S::cen over-expressed cotton shows no difference with wild-type cotton (Fig. 4g), both having a fruiting branch on the tenth node of the main stem, but 35S::CEN over-expressed cotton has a vegetative branch. This result suggests that GhCEN delays the vegetative to reproductive transition.
RNAi silencing of GoCEN promotes cotton floral bud formation
Virus induced gene silencing (VIGS) offers an attractive and quick approach to down-regulate gene expression,and VIGS vectors have been used to identify gene function in cotton [18, 19]. VIGS is environmentally sensitive and fluctuates over time [20, 21]. Therefore, a TRV:CLA treatment was usually used as positive control, which was sensitive and easily observable after the result of blocking chlorophyll production. In this study, numerous newly emerging photo-bleached leaves were showed in all plants inoculated with TRV:CLA after two weeks post inoculation (Additional file 1: Figure S6), which suggested that systemic silencing by TRV in cotton is highly potent. Then, a 241 bp fragment of GoCEN cDNA, corresponding to 793-1013 bp coding bases of GohirD07G113500 from CCRI35, was cloned and inserted into the vector pTRV2. To identify the function of GoCEN in different Gossypium species, we transformed TRV: GhCEN-silencing fragments into G. arboreum, G. barbadense and G. hirsutum (Fig. 5). GoCEN transcript levels were significantly reduced in TRV:GhCEN-silenced cottons of all three species compared with the negative controls (Fig. 5d). The predicted polypeptide sequences were analyzed phylogenetically with TFL1 proteins from cotton, and two other TFL1-like clade, TFL1-L1 and TFL1-L2, shared homology with AtTFL1 . We performed TR-PCR to check the change of the mRNA expression levels for TFL1-L1 and TFL1-L2, and the results showed that there is no difference between wild plants and TRV:CEN-silenced plants (Additional file 1: Figure S7). Floral buds emerged at the fifth node of TRV:CEN-silenced plants, however, no buds were observed in wild-type cotton plants in the three cultivated species. These results suggested that down-regulation of CEN promotes cotton floral bud formation. However, the process of the primary axis on the main stem stops the growth and the first leaf was replaced by a flower bud on the main stem in the CEN-silenced species (Fig. 5). This did not exist in wild-type and cl mutant (Fig. 1). It is well known that FT is transported from leaves to the shoot apex , and LFY acts as a master regulator to orchestrate the whole floral network . Both FT and LFY are required for the differentiation of floral primordium. Therefore, RT-PCR was used to analyze the change of GoFT and GoLYF expression after TRV:CEN-silenced. The result showed that GoLFY significantly enhanced in TRV:CEN-silenced plants than wild plants, while the expression of GoFT in TRV:CEN-silenced plants was a little higher than wild plants (Additional file 1: Figure S7). The result above indicates that GoCEN has a fundamental role in control of the vegetative-reproductive transition and plant architecture.
Down-regulation of GoCEN produces early flowering by activating MADS-box transcription factors
Silencing GoCEN resulted in early production of cotton floral buds (Fig. 5a). The morphology of floral primordia was first observed in the axillary bud when the third true leaf expanded (Fig. 6a). RNA-seq data for the stem apex SAM was used to analyze transcriptional changes between GoCEN-silenced and WT plants, from both G. hirsutum (CCRI35) and G. barbadense (Pima S-6). A total of 1142 common genes showed differential expression between silenced GoCEN and WT plants in the two species (Fig. 6b and Additional file 2: Date S2). As expected, the expression level of GoCEN in GoCEN-silenced plant was lower than in WT plants (about 10 times). GO analyses revealed major enrichment of transcription factor activity (Additional file 1: Figure S8 and Additional file 2: Date S3), suggesting that transcription factors are a basis for flower formation. Further analysis revealed that 37 MADS-box transcription factors may perform important functions in cotton flower development, including 5 AP1, 6 AGL6, 3 SEP2, 2 SEP3, 2 SEP4, 3 AG, 4 AP3 and 4 PI (Additional file 1: Table S3 and Fig. 6c), which had been demonstrated to play pivotal roles in Arabidopsis floral induction and development [24, 25]. KEGG pathway analysis showed that these differentially expressed genes were mainly involved in secondary metabolism, plant hormone signal transduction and starch and sucrose metabolism (Additional file 1: Figure S8). Subsequent RT-PCR confirmed increased expression of some MADS-box transcription factors in CEN-silenced tissues (Additional file 1: Figure S9). We speculated that CEN protein could control the specification and differentiation of flower buds, possibly through regulating the expression of MADS box, hormone or other genes.
Natural variation in GbCEN enhances early maturity
Xinjiang Province is one of the main cotton production areas in China, and is the only region of China where G. barbadense is grown commercially. Compared with other cotton production regions, Xinjiang Province is located at the highest latitudes and had the shortest frost-free days (an average of 180 days per year; Fig. 7a), prompting breeders to select early flowering and maturing cultivars. Surprisingly, we found that almost all cultivars grown in Xinjiang Province are cl mutants (Additional file 2: Date S4), the same as the cl mutants, Hai170 and Xinhai18, which were further confirmed through the pedigree of those varieties (Additional file 1: Figure S10). We hypothesized that the specific natural variant GbCEN could accelerate the early flowering and maturing of cotton. To demonstrate this hypothesis, we collected 51 cl mutant lines and 41 wild type lines from the main Sea-Island cotton growing countries (Additional file 2: Date S5) and measured variation in the time of budding, flowering and maturity between the available mutants and wild types from 2013 to 2016. All cl mutant lines flowered and ripened earlier than wild type lines (Fig. 7b-e), indicating a significant role of cl variation in the adaptation of varieties to high latitudes with short frost-free periods.
Several independent lines of evidence support the assertion that CEN/TFL1 genes, important regulators of plant architecture in multiple species , have been largely responsible for converting cultivated cottons to determinate growth habit, a trait of central importance for mechanical harvest [11, 12] that permitted cotton to become the world’s dominant textile fiber. Genetic mapping of a cl mutant with determinate ‘cluster fruiting’ led to identification of the GoCEN gene. Transgenic evidence, transcriptome and polymorphism analysis show that natural mutation in GoCEN has facilitated cotton architecture and early maturation throughout the history of scientific cotton breeding.
The selective advantage of determinacy associated with GoCEN is highlighted in that several independent mutations have each conferred determinate growth habit to different cotton species and genotypes (Fig. 2). Bradley et al.  and Foucher et al.  described similar situations wherein multiple mutants with indeterminate growth habit were caused by independent mutations in Arabidopsis and peas. A G. barbadense mutation at position 113 in the fourth exon was previously suggested to have a critical role in conferring determinacy . However, to our knowledge, the mutation leading to amino acid substitution of Asp-63 with Asn in the second exon described in this study has not been previously reported in any other species. The nucleobases desertion also resulted in the reading frame shift mutation in Duan063 and Xiaoxian2.This mutation possibly inactivates the GoCEN protein or prevents its interaction with hypothetical cooperator(s) resulting in determinate fruiting branches with cluster fruiting.
In cotton, FD could interact with FT and CEN (TFL1 homolog), and thus antagonize FT activity in the apex and may act as transcriptional repressors of flowering transition, leading to prolonged vegetative growth [30, 31]. Though expression of FT did not significantly increase in the silencing plant, the activity of FT could further enhance interaction with FD and promoter flower. GoCEN may further regulate flowering time through inducing MADS-box and AUX/IAA transcription factors and activating plant hormone signal transduction. CEN-silencing by VIGS promoted the expression of MADS box proteins, including 8 SEP, 6 AGL6, 5 AP1, 4 PI and 3AG (Fig. 6c), that could interact with each other to regulate flowering time and floral organ formation . In contrast, 6 LHY and 4 AUX/IAA transcription factor homologues are down-regulated in GoCEN silenced plants. LHY and SVP interact to delay flowering of Arabidopsis , and AUX/IAA transcription factors cause aberrant cotyledon placement in embryo apical patterning . Sun et al.  also reported multi-hormone signal transduction pathways to be closely related to cotton floral induction. Uncovering the actions of these proteins will provide insight into molecular mechanisms determining plant architecture and flower formation.
Silencing CEN by VIGS conferred a novel phenotype that transgressed the cl mutant, with the main stem terminating in a floral bud in two tetraploid cotton species. This phenotype is consistent with a prior report , and may be due to the combined effects of silencing on homoeologous GoCEN-At and GoCEN-Dt. In other word, a functional GoCEN allele in one of the two tetraploid cotton subgenomes may maintain normal vegetative growth of the main stem apex, while silencing of both homeologs may cause the main stem to terminate in a floral bud. We mapped the cl trait to chromosome 16 in two tetraploid cultivated cottons, as did Chen et al.  and Zhu et al. , but Silow  reported that a cl trait from Pima cotton (G. barbadense) was located on homoeologous chromosome 7. In this study, We show that GoCEN-At and GoCEN-Dt differ by only four nucleotides and have virtually the same functionality through expression and CEN-silenced analysis in G. arboreum (Figs. 2 and 5).
Genetic dosage effects of TFL1/CEN in controlling fruiting type and flowering time in allotetraploid cotton have important implications. TFL1/CEN had been researched in diploid species by positional cloning . However, our discovery of non-additive consequences of silencing multiple, functionally-similar gene family members provide an intriguing example of how polyploid subgenomes have co-evolved following their merger in a common nucleus. Moreover, this finding implies that discovery or engineering of genotypes with additional TFL1/CEN copies (for example, by natural single gene duplication, or artificial genome editing) could result in novel phenotypes, for example conferring extreme determinacy or rapid flowering. This implication suggests new approaches to research on plant architecture and flower formation, as well as new opportunities for seeking or creating novel crop phenotypes using CEN genes.
The close association of cl variation with adaptation of varieties to high latitudes with short frost-free periods suggests opportunities to extend the productive range of cotton and other crops. TFL1/CEN had been reported to play crucial roles in maintaining shoot formation by SAM [11, 27]. In the present study, GhCEN is shown to be highly expressed in the SAM of the main stem and axillary buds, with overexpression of CEN-Dt postponing the floral transition while CEN-silencing advanced flowering in cotton. Thus, cl can accelerate flowering, providing a probable explanation for its abundance in G. barbadense cottons bred for high latitudes and low density early planting. Likewise, introduction of the recessive sp gene into tomato cultivars resulted in determinate growth habit and facilitated mechanical harvesting , and bean and pea also have determinate varieties [37,38,39]. Therefore, the suppression of CEN may provide a strategy for improvement of plant architecture and better adaptation of cotton and other crops to cultivation in regions with short frost-free periods.
In the present study, using a map-based cloning strategy, we have successfully cloned a gene GoCEN-Dt, a homolog of Antirrhinum CENTRORADIALIS, which is responsible for the four natural mutations in the determinate growth habit with cluster fruiting (cl) in cotton. The overexpression of GhCEN-Dt suppresses the transition of the vegetative apex to a reproductive shoot, whereas silencing GoCEN leads to early flowering and determinate growth habit in all apices. We evaluated the importance of the CEN gene for plant architecture and flowering transition in cotton. The cl mutation as marker trait has been selected in cotton breeding in China because of the mutation related with early-maturity. The mutation in CEN gene can increase global cotton production and accelerate flowering, providing a probable explanation for its abundance in cotton bred for high latitudes and high density early planting.
Plant materials and growth conditions
Two G. barbadense cluster fruiting mutants, Xinhai18 and Junhian1 of and two G. hirsutum cluster fruiting mutants Chaozao3 and Xiaoxian2, were provided by the National Medium-term Gene Bank of Cotton in China and National cotton germplasm resources platform. Cluster fruiting mutant Hai170 was provided by the Agricultural Sciences Institute for the seventh divisions of Xinjiang Production and Construction Corps. Gossypium hirsutum cultivar CCRI35 and G. barbadense line Hai170 were chosen to produce the segregating population. CCRI 35, a normal fruiting type, was widely planted in China in the last decade. The population was developed in summer 2011 at Southwest University (SWU), Chongqing, China. Individual F1 plants were self-pollinated and F2 seeds were harvested in winter 2011 in Sanya, Hainan, China. Parents and F2 segregating population were planted in single-row plots 0.7 m wide and 5 m long, in 2012 and 2013 at Southwest University (SWU), Chongqing, China.
An additional 92 accessions collected from the main Sea Island cotton growing countries, including 51 cl mutant lines and 41 normal type lines, were grown in Korla, Xinjiang Province, China from 2013 to 2016 and were used to investigate the relationship between the type of fruiting branch and maturity (Additional file 2: Data S5).
Map-based cloning of GoCEN
Genomic DNA from parents and the segregating population was isolated by CTAB method. Simple sequence repeat (SSR) primers with prefix SWU are described in Additional file 2: Data S1. All primers were synthesized by Invitrogen (Shanghai, China). Genotyping using SSR markers was done as described . Only the clear DNA bands on the gels were chosen for scoring and genotypes were scored according to the coding system described in the JoinMap4.0 Manual.
Based on the G. raimondii genome , Gorai.001G121800 was amplified with primers encompassing the coding regions. The 25-μl PCR reactions included 50 ng cotton genomic DNA or cDNA, 1 × PrimerSTAR mix (TaKaRa), and 200 nM upstream and downstream primers. The primers were listed in Additional file 2: Data S5. GoCEN genomic sequences were amplified from two diploid (G. arboreum, G. raimondii) and two tetraploid species (G. hirsutum, G. barbadense). The PCR thermal cycling parameters were: 98 °C for 1 min, followed by 35 cycles of 98 °C for 10s, 55 °C for 15 s and 72 °C for 30s, and a final extension of 5 min at 72 °C. PCR products were cloned into PDM19-T vector (TaKaRa) and sequenced by Invitrogen (Shanghai, China). In tetraploid cotton, GoCEN has two homeologs (one similar to the diploid D genome and the other to the A genome), named GoCEN-Dt and GoCEN-At, respectively.
Plasmid construction and plant transformation
To construct overexpression vectors, fragments of the full length CEN-Dt from CCRI35 and Hai170 were cut from PDM19-T with EcoRI and SmaI restriction enzymes, gel purified, and cloned into pPLGN-35S-MCS-Nos to produce the constitutive overexpression constructs.
Overexpression constructs were transformed into Agrobacterium tumefaciens (LBA4404), and the resulting strains were used to produce transgenic cottons. Jimian 14 cotyledons were used as explants for transformations according to the protocol of Luo et al.  Transgenic plantlets were identified using histochemical GUS staining in leaf tissues, and GUS-positive plants were transplanted and grown in the greenhouse. PCR was used to confirm the presence of transgenes in transformants and progeny of transgenic lines.
VIGS of GoCEN in cotton
A GhCEN cDNA fragment was cloned from the SAM of Upland cotton CCRI35 vegetative shoots. A 241-bp fragment from the fourth exon was amplified from cDNA. The primers were listed in Additional file 2: Data S1. The product was cloned into PDM19-T vector (TaKaRa) and sequenced by Invitrogen (Shanghai, China). EcoRI and KpnI inserts were cloned into TRV2, generating a TRV2-GoCEN vector. Binary vectors (TRV1 and TRV2-GhCEN) were introduced into Agrobacterium tumefasciens strain GV3101 by electroporation. Three cultivated species, G. arboreum, G. barbadense and G. hirsutum, were infiltrated with a 1:1 mixture of Agrobacterium carrying pTRV1 and TRV2 as a negative control, or pTRV1 and TRV2-GOCEN at 1 week post-germination using a 1-ml syringe. The VIGS assay was used to inoculate cultures as described . Inoculated plants were first kept for 48 h (hour) at room temperature in darkness, then transplanted into a growth chamber under a 26/22 °C day/night cycle in long-day conditions (16 h/8 h, light/dark).
RNA sample extraction and real-time PCR analysis
Total RNAs were extracted from roots, stems, leaves, petals, ovules and SAM, using a rapid plant RNA extraction kit (Aidlab, Beijing, China). The cDNAs were synthesized from total RNA using a first-strand cDNA synthesis kit (TaKaRa, Dalian, China), and then subjected to real-time PCR analyses. Real-time PCRs were performed on a CFX96 real-time PCR detection system using SYBR Green Supermix (Bio-Rad, CA, USA) according to the manufacturer’s introductions. The thermal cycling parameters were: 95 °C for 2 min, followed by 40 cycles of 95 °C for 10 s, 57 °C for 20 s, followed by a standard melting curve to monitor PCR specificity. The primers are listed in Additional file 2: Data S1. Data were analyzed using the software Bio-Rad CFX Manager 2.0 provided by the manufacturer.
In situ hybridization
A 221-bp gene-specific CEN probe was amplified with the primers Prob-CEN-F and Prob-CEN-R (Additional file 1: Data S1) and labeled using the DIG RNA Labeling Kit (SP6/T7; Roche), following the manufacturer’s recommendations. Pretreatment of sections, hybridization and immunological detection were performed using published methods . Source tissue was obtained from the tips of main stems, including embryonic leaves, apex tissues and non-elongated internodes at the full-bloom stage.
The coding regions of GoCEN-At and GoCEN-Dt without the stop codon were amplified from CCRI35 cDNA (G. hirsutum) using primers (Additional file 2: Data S1). The following constructs were obtained and confirmed by sequencing: 35S::GhCEN-At-GFP and 35S::GhCEN-Dt-GFP. Both constructs were introduced into N. benthamiana plants by Agrobacterium tumefasciens GV3101. After 48 h incubation, GFP fluorescence and DAPI was observed and co-localized by confocal laser scanning microscopy (FV1000; Olympus, Tokyo, Japan).
Shoot apical meristem (SAM) tissue was used for RNA-seq to identify DEGs in CEN silenced G. hirsutum and G. barbadense. Flower bud differentiation is first observed at the expansion of the third true leaf using VIGS of CEN in G. hirsutum and G. barbadense (Fig. 6a). After a period of growth in chamber, SAMs without leaves from G. hirsutum CCRI35, CEN silenced CCRI35 and G. barbadense Pima S-6, CEN silenced Pima S-6 were collected for total RNA extraction. Two biological replicates were performed. RNA extraction, detection and library construction followed our published method . Library preparations were sequenced on an Illumina Hiseq platform by Novogene Bioinformatics Institute (Beijing, China) and 125 bp/150 bp paired-end reads were generated. The clean reads were obtained by removing reads containing adapter, reads containing ploy-N and low quality reads from raw data. The clean sequence tags were mapped to a G. hirsutum (TM-1) genome  using TopHat v2.0.12 . FPKM  (Fragments Per Kilo base of exon per Million fragments mapped) was used to screen differentially expressed genes (DEGs) between pairwise comparisons (CEN-silenced CCRI35 versus CCRI35 and CEN-silenced Pima S-6 versus Pima S-6). Differentially expressed genes (FDR adjusted P value ≤0.05 and log2 fold change ≥1) were identified by performing a pair wise comparison. Gene Ontology (GO) terms with corrected P value less than 0.05 were considered significantly enriched using GOseq R package . Pathway analysis was mainly based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database .
- CEN :
Differentially expressed genes
Green fluorescent protein
Kyoto Encyclopedia of Genes and Genomes
Quantitative real-time PCR
Shoot apical meristems
Simple sequence repeat
Virus induced gene silencing
Chen ZJ, Scheffler BE, Dennis E, Triplett BA, Zhang T, Guo W, Chen X, Stelly DM, Rabinowicz PD, Town CD, et al. Toward sequencing cotton (Gossypium) genomes. Plant Physiol. 2007;145(4):1303–10.
Wendel JF, Grover CE. Taxonomy and evolution of the cotton genus, Gossypium. In: Fang DD, Percy RG, editors. Cotton. 2nd. Agronomy Monograph; 2015. p. 25–44.
Fang L, Wang Q, Hu Y, Jia Y, Chen J, Liu B, Zhang Z, Guan X, Chen S, Zhou B, et al. Genomic analyses in cotton identify signatures of selection and loci associated with fiber quality and yield traits. Nat Genet. 2017;49(7):1089–98.
Pettigrew WT. Source-to-sink manipulation effects on cotton lint yield and yield component. Agron J. 1994;86:731–5.
Jiao Y, Wang Y, Xue D, Wang J, Yan M, Liu G, Dong G, Zeng D, Lu Z, Zhu X, et al. Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice. Nat Genet. 2010;42:536–41.
Thompson AM, Yu J, Timmermans MCP, Schnable P, Crants JC, Scanlon MJ, Muehlbauer GJ. Diversity of Maize Shoot Apical Meristem Architecture and Its Relationship to Plant Morphology. G3-GENES GENOM GENET. 2015;5(5):819–27.
Wang Y, Li J. Molecular basis of plant architecture. Ann Rev Plant Biol. 2008;59:253–79.
Imaizumi T, Kay SA. Photoperiodic control of flowering: not only by coincidence. Trends Plant Sci. 2006;11:550–8.
Chen W, Yao J, Chu L, Yuan Z, Li Y, Zhang Y. Genetic mapping of the nulliplex-branch gene (gb_nb1) in cotton using next-generation sequencing. Theor Appl Genet. 2015;128(3):539–47.
McGarry RC, Prewitt SF, Culpepper S, Eshed Y, Lifschitz E, Ayre BG. Monopodial and sympodial branching architecture in cotton is differentially regulated by the Gossypium hirsutum SINGLE FLOWER TRUSS and SELF-PRUNING orthologs. New Phytol. 2016;212(1):244–58.
Pnueli L, Carmel-Goren L, Hareven D, Gutfinger T, Alvarez J, Ganal M, Zamir D, Lifschitz E. The SELF-PRUNING gene of tomato regulates vegetative to reproductive switching of sympodial meristems and is the ortholog of CEN and TFL1. Development. 1998;125(11):1979–89.
Reta-Sanchez DG, Fowler JL. Canopy light environment and yield of narrow-row cotton as affected by canopy architecture. Agron J. 2002;94:1317–23.
Endrizzi JE, Turcotte EL, Kohel RJ. Qualitative genetics, cytology, and cytogenetics. In: Kohel RJ, Lewis CF (eds) Cotton. Agronomy Society of America, Madison; 1984. p. 81–p119.
Wang W, Tan Z, Xu Y, Zhu A, Li Y, Yao J, Tian R, Fang X, Liu X, Tian Y, et al. Chromosome structural variation of two cultivated tetraploid cottons and their ancestral diploid species based on a new high-density genetic map. Sci Rep. 2017;7(1):7640.
Paterson AH, Wendel JF, Gundlach H, Guo H, Jenkins J, Jin D, Llewellyn D, Showmaker KC, Shu S, Udall J, et al. Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres. Nature. 2012;492(7429):423–7.
Zhang T, Hu Y, Jiang W, Fang L, Guan X, Chen J, Zhang J, Saski CA, Scheffler BE, Stelly DM, et al. Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement. Nat Biotechnol. 2015;33(5):531–7.
Bradley D, Carpenter R, Copsey L, Vincent C, Rothstein S, Coen E. Control of inflorescence architecture in Antirrhinum. NATURE. 1996;379(6568):791–7.
Ma D, Hu Y, Yang C, Liu B, Fang L, Wan Q, Liang W, Mei G, Wang L, Wang H, et al. Genetic basis for glandular trichome formation in cotton. Nat Commun. 2016;7:10456.
Andres RJ, Coneva V, Frank MH, Tuttle JR, Samayoa LF, Han SW, Kaur B, Zhu L, Fang H, Bowman DT, et al. Modifications to a late meristem identity1 gene are responsible for the major leaf shapes of upland cotton (Gossypium hirsutum L.). Proc Natl Acad Sci U S A. 2017;114(1):E57–66.
Fu DQ, Zhu BZ, Zhu HL, Zhang HX, Xie YH, Jiang WB, Zhao XD, Luo KB. Enhancement of virus-induced gene silencing in tomato by low temperature and low humidity. Mol Cells. 2006;21(1):153–60.
Senthil-Kumar M, Mysore KS. Virus-induced gene silencing can persist for more than 2 years and also be transmitted to progeny seedlings in Nicotiana benthamiana and tomato. Plant Biotechnol J. 2011;9(7):797–806.
Li C, Zhang Y, Zhang K, Guo D, Cui B, Wang X, Huang X. Promoting flowering, lateral shoot outgrowth, leaf development, and flower abscission in tobacco plants overexpressing cotton FLOWERING LOCUS T (FT)-like gene GhFT1. Front Plant Sci. 2015;17(6):454.
Moyroud E, Kusters E, Monniaux M, Koes R, Parcy F. LEAFY blossoms. Trends Plant Sci. 2010;15(6):346–52.
Fernandez-Nohales P, Domenech MJ. Martinez de Alba AE, Micol JL, Ponce MR, Madueno F. AGO1 controls arabidopsis inflorescence architecture possibly by regulating TFL1 expression. Ann Bot. 2014;114(7):1471–81.
de Folter S, Immink RG, Kieffer M, Parenicová L, Henz SR, Weigel D, Busscher M, Kooiker M, Colombo L, Kater MM, et al. Comprehensive interaction map of the Arabidopsis MADS box transcription factors. Plant Cell. 2005;17(5):1424–33.
Wickland DP, Hanzawa Y. The flowering locus t/terminal flower 1 gene family: functional evolution and molecular mechanisms. Mol Plant. 2015;8(7):983–97.
Bradley D, Ratcliffe O, Vincent C, Carpenter R, Coen E. Inflorescence commitment and architecture in Arabidopsis. Science. 1997;275(5296):80–3.
Foucher F, Morin J, Courtiade J, Cadioux S, Ellis N, Banfield MJ, Rameau C. Determinate and late flowering are two terminal flower1/centroradialis homologs that control two distinct phases of flowering initiation and development in pea. Plant Cell. 2003;15(11):2742–54.
Ahn JH, Miller D, Winter VJ, Banfield MJ, Lee JH, Yoo SY, Henz SR, Brady RL, Weigel D. A divergent external loop confers antagonistic activity on floral regulators FT and TFL1. EMBO J. 2006;25(3):605–14.
Si ZF, Liu H, Zhu JK, Chen JD, Wang Q, Fang L, Gao FK, Tian Y, Chen YL, Chang LJ, et al. Mutation of SELF-PRUNING homologs in cotton promotes short-branching plant architecture. J Exp Bot. 2018;69(10):2543–53.
Huang NC, Jane WN, Chen J, Yu TS. Arabidopsis thaliana centroradialis homologue (ATC) acts systemically to inhibit foral initiation in Arabidopsis. Plant J. 2012;72:175–84.
Fujiwara S, Oda A, Yoshida R, Niinuma K, Miyata K, Tomozoe Y, Tajima T, Nakagawa M, Hayashi K, Coupland G, et al. Circadian clock proteins LHY and CCA1 regulate SVP protein accumulation to control flowering in Arabidopsis. Plant Cell. 2008;20(11):2960–71.
Ploense SE, Wu MF, Nagpal P, Reed JW. A gain-of-function mutation in IAA18 alters Arabidopsis embryonic apical patterning. Development. 2009;136(9):1509–17.
Sun Q, Du X, Cai C, Long L, Zhang S, Qiao P, Wang W, Zhou K, Wang G, Liu X, et al. To be a flower or fruiting branch: insights revealed by mRNA and small RNA transcriptomes from different cotton developmental stages. Sci Rep. 2016;6:23212.
Zhu J, Chen J, Gao F, Xu C, Wu H, Chen K, Si Z, Yan H, Zhang T. Rapid mapping and cloning of the virescent-1 gene in cotton by bulked segregant analysis-next generation sequencing and virus-induced gene silencing strategies. J Exp Bot. 2017;68(15):4125–35.
Silow RA. Evidence on chromosome homology in the amphidiploid New World cottons. J Genet. 1946;47:213–21.
Cober ER, Tanner JW. Performance of related indeterminate and tall determinate soybean lines in short-season areas. Crop Sci. 1995;35(2):361–4.
Tian Z, Wang X, Lee R, Li Y, Specht JE, Nelson RL, McClean PE, Qiu L, Ma J. Artificial selection for determinate growth habit in soybean. P Natl Acad Sci USA. 2010;107(19):8563–8.
Dhanasekar P, Reddy KS. A novel mutation in TFL1 homolog affecting determinacy in cowpea (Vigna unguiculata). Mol Gen Genomics. 2015;290(1):55–65.
Liu D, Zhang J, Liu X, Wang W, Liu D, Teng Z, Fang X, Tan Z, Tang S, Yang J, et al. Fine mapping and RNA-Seq unravels candidate genes for a major QTL controlling multiple fiber quality traits at the T1 region in upland cotton. BMC Genomics. 2016;17(1):295.
Luo M, Xiao Y, Li X, Lu X, Deng W, Li D, Hou L, Hu M, Li Y, Pei Y. GhDET2, a steroid 5 alpha-reductase, plays an important role in cotton fiber cell initiation and elongation. Plant J. 2007;51(3):419–30.
Tuttle JR, Haigler CH, Robertson D. Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system. Plant Methods. 2012;8(1):27.
Zhang M, Zheng X, Song S, Zeng Q, Hou L, Li D, Zhao J, Wei Y, Li X, Luo M, et al. Spatiotemporal manipulation of auxin biosynthesis in cotton ovule epidermal cells enhances fiber yield and quality. Nat Biotechnol. 2011;29(5):453–8.
Kim D, Pertea G, Trapnell C, Pimentel H, Kelley R, Salzberg SL. TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions. Genome Biol. 2013;14(4):R36.
Wang L, Feng Z, Wang X, Wang X, Zhang X. DEGseq: an R package for identifying differentially expressed genes from RNA-seq data. Bioinformatics. 2010;26(1):136–8.
Young MD, Wakefield MJ, Smyth GK, Oshlack A. Gene ontology analysis for RNA-seq: accounting for selection bias. Genome Biol. 2010;11(2):R14.
Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M, Katayama T, Kawashima S, Okuda S, Tokimatsu T, et al. KEGG for linking genomes to life and the environment. Nucleic Acids Res. 2008;36(Database issue):D480–4.
This work was designed by the National Key Research and Development Program of China (grant No. 2016YFD0100203–2), and the National Science Foundation of China (grant No. 31701471 and 31460363) had role in the work for data collection and analysis and preparation of the manuscript.
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The datasets supporting the conclusions of this article are included within the article and its Additional files. The RNA-seq data have been deposited in the NCBI Sequence Read Archive under accession number PRJNA406850.
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Figure S1. Plant morphologies of WT and mutants with cl trait in G. barbadense and G. hirsutum. Figure S2. Fine mapping of Gb-cl. Figure S3 Gnentic mapping of Gh-cl. Figure S4. Phylogenetic tree of TFL1-related proteins constructed using neighbor-joining method with the program MEGA 5.10 in tree view. Figure S5. The nucleotide sequence alignment of GoCEN genesfrom A-subgenome and D-subgenome from cotton cultivars/lines used in this study. Figure S6. Functional characterization of GoCLA by VIGS. Figure S7. Genes expression level with RT-PCR in G. hirsutum and G. barbadense between CEN-silenced and WT plant. Figure S8. GO and KEGG enrichment analysis of differentially expressed genes screening from WT and GoCEN silenced plant in G. hirsutum and G. barbadense. Figure S9. qRT-PCR validation of MADS-box transcription factors in G. hirsutum and G. barbadense between CEN-silenced and WT plant. Figure S10. The pedigree of G. barbadense commercial cultivars in Xinjiang Province. Table S1. Thirty-six candidate genes for Gh-cl and their putative function. Table S2. Nucleotide sequence variations between GoCEN-A07 and GoCEN-D07 in tetraploid cotton. Table S3. MADS-box transcription factors differentially expressed in transcriptome. (DOCX 5482 kb)
Data S1. All primers used in this study. Data S2. The list of significant differentially expressed genes in Gh_VIGSvsGh_WT_Gb_VIGSvsGb_WT.venn. Data S3. 174 transcription factors in Gh_VIGSvsGh_WT_Gb_VIGSvsGb_WT.venn. Data S4. The 58 cultivars bred in Xinjiang Province. Data S5. The information of the expermental material of G. barbadense. (XLSX 167 kb)
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Liu, D., Teng, Z., Kong, J. et al. Natural variation in a CENTRORADIALIS homolog contributed to cluster fruiting and early maturity in cotton. BMC Plant Biol 18, 286 (2018). https://doi.org/10.1186/s12870-018-1518-8
- Map-based cloning
- Cluster fruiting
- Determinate growth habit
- Plant architecture
- Early maturity