Fig. 5From: A biolistic method for high-throughput production of transgenic wheat plants with single gene insertionsBiolistic wheat transformation process with hygromycin selection. a Freshly isolated immature embryo; (b) 2 week-old embryogenic callus before bombardment; (c) Shoot regeneration on 50Â mg/L hygromycin selection medium; (d) Shoot and root regeneration on 50Â mg/L hygromycin selection medium; (e) Plantlet recovery in 1/2 MS medium; (f) T0 transgenic plants in the greenhouseBack to article page