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Table 2 List of primer pairs used to isolate the α-type gliadins and in RT-PCR analysis

From: Production and molecular characterization of bread wheat lines with reduced amount of α-type gliadins

Primer name Sequence Reference
L1 5’-ATGAAGACCTTTCTCATCCTTG Xie et al. [35]
R1 5’-TCAGTTRGTACCRAAGATGCC
RT a-gli F 5’-AGACCTTTCTCATCCTTGCC Li et al. [36]
RT a-gli R 5’-TGTACCAATGGAACTTGCTCT
G1Gli*1 5’-ACAGGTGAACCCATGCAAGAATTT Piston et al. [37]
G1Gli*2 5’-TGCATGATGATGGAATGTATGATGG
G2Gli*1 5’-TCATTCCCCCAACAACAACGG Piston et al. [37]
G2Gli*2 5’-AGGTTTGCATTGTTGCAAGAGGAT
G3 Gli*1 5’-GCAAATCCTGGTGCCACTGTCTCAA Piston et al. [37]
G3 Gli*2 5’-TGGGACATACACGTTGCACATGGTT  
G4 Gli*1 5’-GATCCTGCGGCCACTATTTCAGCTC Piston et al. [37]
G4 Gli*2 5’-CAGGTGGCACATACACGTTGCACAT  
QF18 5’-AAGGCAAGCAAGCAGTAG Altenbach et al. [38]
QR18 5’-GATTGTTGAGGTGATTGTAGC
QF21 5’-CAACCACCACAACAATTC Altenbach et al. [38]
QR21 5’-TTACATCTCTTCATTTCATAGG