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Table 3 Primers sequences constructed and used in qRT-PCRs

From: Metabolic pathways and genes identified by RNA-seq analysis of barley near-isogenic lines differing by allelic state of the Black lemma and pericarp (Blp) gene

Gene

Product

Primers sequences

MLOC_16300

Apyrase 3

Forward 5’CCTATGGGTTGCTCTGAATTAC3’, Reverse 5’AGTAGTCTCCTCCCAGTTTAC3’

MLOC_51393

Isoflavone reductase

Forward 5’GCTTTCTTCCCGCATTCT3’, Reverse 5’GATACGATGGCAGATGAACTAA3’

MLOC_55029

Lipoxygenase B

Forward 5’ACAGAAACGGACCCTAGAT3’, Reverse 5’CCTGGTTATCGGAAGAATGG3’

MLOC_76018

Caffeic acid O-methyltransferase

Forward 5’CAAGAAGTACCCGAGCATAAA3’, Reverse 5’CGGCACCATCTCAAACAT3’

MLOC_62322

Phenylalanine ammonia-lyase

Forward 5’GCTCATGTTTGCCCAATTC3’, Reverse 5’GGAAAGGTTGGAAGGTAGAC3

MLOC_80301

Polyphenol Oxydase

Forward 5’AGCAAGGAGAAGGAGGAG3’, Reverse 5’GCACGTCGAACTTGATGA3’

MLOC_3802

Universal Stress Protein

Forward 5’CCGGAGGTGATGAAGAACTA3’, Reverse 5’ATGACGATGGTGTCGATCT3’

MLOC_54913

Lys-63-specific deubiquitinase

Forward 5’CGGCAAGACATCTACCAATAG3’, Reverse 5’GCACTGTTATCCGGTTCATAA3’