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Impact of the 7-bp deletion in HvGA20ox2 gene on agronomic important traits in barley (Hordeum vulgare L.)
BMC Plant Biology volume 17, Article number: 181 (2017)
Alike to Reduced height-1 (Rht-1) genes in wheat and the semi dwarfing (sd-1) gene in rice, the sdw1/denso locus involved in the metabolism of the GA, was designated as the ‘Green Revolution’ gene in barley. The recent molecular characterization of the candidate gene HvGA20ox2 for sdw1/denso locus allows to estimate the impact of the functional polymorphism of this gene on the variation of agronomically important traits in barley.
We investigated the effect of the 7-bp deletion in exon 1 of HvGA20ox2 gene (sdw1.d mutation) on the variation of yield-related and malting quality traits in the population of DHLs derived from cross of medium tall barley Morex and semi-dwarf barley Barke. Segregation of plant height, flowering time, thousand grain weight, grain protein content and grain starch was evaluated in two diverse environments separated from one another by 15° of latitude. The 7-bp deletion in HvGA20ox2 gene reduced plant height by approximately 13 cm and delayed flowering time by 3–5 days in the barley segregating DHLs population independently on environmental cue. On other hand, the sdw1.d mutation did not affect significantly either grain quality traits (protein and starch content) or thousand grain weight.
The beneficial effect of the sdw1.d allele could be associated in barley with lodging resistance and extended period of vegetative growth allowing to accumulate additional biomass that supports higher yield in certain environments. However, no direct effect of the sdw1.d mutation on thousand grain weight or grain quality traits in barley was detected.
The tremendous success of the “Green Revolution” of the XX century was achieved due to the introduction in agriculture the new cereal varieties distinguished by a shortened thick stem, resistant to lodging. These varieties made it possible to change agricultural technology, to increase the dose of mineral fertilizers, and, as a result, dramatically improved the productivity of cereal crops. For wheat and rice the “Green Revolution” genes were involved in a gibberellic acid (GA) metabolism influencing plant height (dwarfing), lodging resistance, and, consequently, harvest index (e.g. ). In hexaploid wheat, dwarfing has been achieved mainly through the introduction of the mutant alleles of Reduced height-1 (Rht-1) genes that encode DELLA proteins, which act to repress GA-responsive growth . In rice, the record yields obtained in the 1960s throughout Asia were owing to semi-dwarfing sd-1 mutants carrying a deletion of 280 bp within the coding region of Os20ox2 gene resulting in the nonfunctional 20-oxidase GA biosynthetic enzyme . Thus, the genes involved in the metabolism of GA (semi dwarfing sd-1 gene in rice and Rht gene in wheat) have played the crucial role in plant height reduction, which led to a significant increase in yields of the two major cereal crops. In barley, however, the impact of the semi-dwarfing genes on agronomical success of modern varieties still remains uncertain.
Two dwarfing genes, sdw1/denso and uzu1.a involved, respectively, in the metabolism of the GA and brassinosteroid hormones, were designated as “the Green Revolution” genes in barley . Among them, the sdw1 barley gene is an ortholog of the semi-dwarf sd-1 gene in rice; the corresponding candidate gene HvGA20ox2 encoding the gibberellin 20-oxidase was recently reported [5, 6]. Four mutant alleles of sdw1 gene are known in barley. One of them sdw1.c (originally named denso) was found in a spontaneous mutant selected from the cv. Abed Denso in Denmark. Three other sdw1 alleles have been obtained artificially with the use of mutagens: 1) sdw1.a mutant (originally named sdw1) were induced by X-rays in the Norwegian six-rowed barley cv. Jotun. 2) the sdw1.d mutant was obtained using X-rays treatment of cultivar Valticky and released in 1965 as cv. Diamant. Today cultivars with the sdw1.d mutation from Diamant are often described as «sdw1/denso». 3) sdw1.e named as ‘Risø no. 9265’ was isolated in a M2 generation from the variety Bomi treated by neutrons .
A pronounced success was achieved in molecular characterization of the sdw1 barley alleles [5,6,7,8]. First, HvGA20ox2 sequences of 2413 bp were compared between medium tall barley variety AC Metcalfe and semi-dwarf variety Baudin possessing the semi-dwarfing gene (sdw1.d); the A/G substitution was identified in the intron 2 co-segregating with plant height in AC Metcalfe/ Baudin DH progeny . Next, the 7-bp deletion in exon 1 resulting in coding frame shifts of HvGA20ox2 gene was suggested as the functional polymorphism of the sdw1.d allele from Diamant . A complete deletion of HvGA20ox2 gene was identified in semi-dwarf mutant Riso no. 9265 carrying sdw1.e allele, originated from the variety Bomi . Five different sequence variations were identified by comparing the HvGA20ox2 gene sequence of the sdw1.c mutant (Abed Denso) with the tall barley cultivars: they include a 1-bp deletion and a 4-bp insertion in the 5′ untranslated region as well as two synonymous mutations in coding sequence of HvGA20ox2 gene . While plant height variation was significantly associated with the 7-bp deletion of HvGA20ox2 gene (sdw1.d allele) in a bi-parental mapping population and a natural population of barley varieties, none of the exposed sequence variations the sdw1.c mutants could explain dwarf phenotype .
All the sdw1 barley mutants are distinguished by shorter and stronger culms supporting spikes and preventing lodging. However, the mutations in HvGA20ox2 gene affect biosynthesis of the GA that control many processes in different plant tissues, thus, their pleiotropic effects also cause a number of unwanted agronomic traits such as reduction of grain size . Both beneficial and deleterious effects associated with sdw1 gene were reported for barley (reviewed in ). Recent success in molecular characterization of the sdw1 alleles facilitated further studies focusing on the better understanding of their pleiotropic effect on agronomic performance of barley varieties.
In the present paper we estimated the effect causing by the 7-bp deletion in exon 1 of HvGA20ox2 gene (the sdw1/denso allele) on the variation of economically important traits in the segregating population of the double haploid lines (DHLs) derived from the cross of the tall barley Morex and the semi-dwarf sdw1.d mutant cv. Barke. Barke inherited the sdw1/denso allele from Triumph via cv. Alexis (https://triticeaetoolbox.org/barley/), while Triumph is a descendant of cv. Diamant. We estimated the phenotypic effect of the sdw1/denso allele in two very diverse environments by the QTL mapping approach. Variation of plant height, heading date, thousand grain weight, grain protein and starch content in seeds was evaluated.
Plant material and field evaluation
Seeds of double haploid lines (DHLs) derived from the cross between Morex and Barke were kindly offered for the field evaluation by Nils Stein (Gatersleben, Germany). The DHLs population is abbreviated further in the text as Morex/Barke DHLs population.
Field trials of the Morex/Barke DHLs population were conducted in two experimental stations of VIR: in Pushkin (59°53′39 ″N, St. Petersburg region, North of Russia) and Krasnodar (45°02′55 ″N, South of Russia). The field experiment consisted of two replicate plots each containing a row of 50 plants of each doubled haploid line and two rows of each parent. Rows were spaced approximately 20 cm apart.
DHLs were evaluated for heading date (HD) that was calculated as number of days from sowing until first awns visible. Plant height (PH) was measured at full maturity: height to top of spike excluding awns was recorded for ten individuals per each DH line. PH records for Morex/Barke DHLs were obtained in 2015 (Pushkin).
Thousand grain weight (TGW) was measured at the conditioning humidity, according to standard protocols : from the sample of grains for each DH line two weights were singled out, each with 500 seeds. The samples were weighed on the laboratory balances with an accuracy of 0.01 g. The sum of the results of weighing of two samples of 500 seeds was calculated.
Grain Protein Content (GPC) and starch content in dry seeds were analyzed using Infratec 1241 Grain Analyser (Foss, USA). The data to construct the calibration curve for GPC were obtained with Kjeltec Auto 1030 Analyzer (Sweden) by Kjeldahl method. The calibration curve for starch determination was developed with Evers’ Polarimetric Method.
QTL mapping and statistical analysis
For the Morex/Barke segregating population a comprehensive genetic map was available consisting of 1068 SNP markers for 93 DHLs , the published genotyping data were used for the QTL analysis. Mapping of QTL was carried out with the Windows QTL Cartographer version 2.5 software  using the CIM algorithm (Composite Interval Mapping). The minimum threshold of LOD, significant at 95% (p = 0.05), was calculated based on the results of 1000 permutations. To visualize LOD scans a Perl script was used (available upon request).
CAPS assay to reveal 7 bp deletion in exon 1 of HvGA20ox2 gene
PCR was performed with designed primers: forward 5′-CTCCCTCCCTCCCCGATTAC and reverse 5′-CCGGACACCTGGAAGAACCC. The reaction mix contained 1 × Taq-buffer (2.5 mM Mg2+); 200 μM dNTP; 2,5 U Tag DNA polymerase (Sileks, Moscow); 0.4 μM of each primer; ~ 30 ng of template DNA, 5% DMSO and sterile distilled water in a final volume of 25 μl. PCR cycling conditions consisted of an initial denaturation step of 95 °C for 3 min, followed by 30 cycles of 95 °C for 20 s, 63 °C for 30 s, 72 °C for 45 s, and a final extension cycle at 72 °C for 5 min. PCR products were detected on 1,3% agarose gel in TBE buffer, then were purified with ISOLATE II PCR and Gel Kit (Bioline) and sequenced in two ends (Eurogen, Moscow) using forward and reverse primers. The DNA sequences were aligned with Unipro UGENE software . GenBank sequence accessions are: ‘Barke’ KX611232, ‘Triumph’ KX611233, ‘Morex’ KX611234, ‘Franklin’ KX789375.
To reveal the 7 bp deletion in exon 1 of HvGA20ox2 gene 3 μl of the PCR product were digested with 1 U of Hinf I (Sibenzyme, Novosibirsk) in a total reaction volume of 15 μl containing 1х SEbuffer O (pH 7.6) for 2 h at 37 °C, followed by electrophoretic separation in 1.5% agarose gel in TBE buffer.
Re-sequencing and mapping of HvGA20ox2 gene in Morex/Barke DHLs population
To prove that Barke is the sdw1.d mutant we re-sequenced the exon 1 of HvGA20ox2 gene for the barley variety. To design primers we aligned the morex_contig_40861 (http://webblast.ipk-gatersleben.de/barley/viroblast.php) containing the published fragment of HvGA20ox2 sequence and MLOC_56462.1 that is the coding sequence for HvGA20ox2 gene (Fig. 1a). The PCR fragment of 488 bp was successfully amplified and sequenced for Barke, Triumph and Franklin barley varieties which are derived from the barley ‘Diamant family’ carrying semi-dwarf sdw1/denso allele, and also for Morex (tall barley). Deletion of 7 bp leading to the shift of reading frame in Barke, Triumph and Franklin was confirmed in exon 1 of HvGA20ox2. The intact and altered predicted proteins have just 34 amino acid sequence in common, followed by completely different polypeptides (Fig. 1b).
To map the HvGA20ox2 gene we developed the gene specific CAPS marker. The CAPS marker exposes whether a DH line has the 7-bp deletion (Barke allele) or carries intact exon 1 (Morex allele) (Fig. 2). Next, 94 DHLs from the cross of Morex and Barke were assessed with the gene-specific CAPS marker. As the result, the HvGA20ox2 gene was mapped in the Morex/Barke DHLs population on the long arm of 3H chromosome in the position of 132.7 cM, co-segregating with SNP 1_0754 (Additional file 1).
7-bp deletion in exon 1 of HvGA20ox2 associates with plant height and heading date segregation in Morex/Barke DHLs population
Segregation of plant height in Morex/Barke DHLs population
To verify whether the sdw1.d mutation is associated with the plant height segregation observed in the Morex/Barke DHLs population, the height of 920 plants (ten individual plants per each of 90 DHLs and two parents (Additional file 2) were compared considering the 7-bp deletion in exon 1 of the HvGA20ox2 gene. The resulting bimodal distribution is shown in Fig. 3a. Two distinct peaks reflect significant difference between plant height values of the two allele classes (p < 0.0000001). For DH lines with Barke sdw1.d allele the averaged plant height varied from 50 to 87 cm with a mean value of 70 cm; for DH lines with Morex allele plant height ranged from 60 to 107, an average 83 cm. It was previously reported that the sdw1 and denso allele reduced height by 10 to 20 cm [5, 14, 15].
The plant height variation was also subjected to QTL mapping. As the result, the highly significant QTL peak (LOD = 10.6, p < 0.05) was mapped on 3H in Morex/Barke DHLs population, co-segregating with the HvGA20ox2 gene specific CAPS marker and explaining 31% of observed phenotypical variation (Fig. 3b).
Segregation of heading date in Morex/Barke DHLs population
The Morex/Barke DHLs population was evaluated for heading date segregation in five environments: three years (2011–2013) in Pushkin and two years (2012, 2013) in Krasnodar. The two geographical locations are separated from one another by 15° of latitude; the day length on the date of seedlings emergence in 2012 was 17 h 30 min and 13 h 24 min respectively. The averaged heading date of the DH lines in the short day conditions (Krasnodar) was 12–20 days longer than in long day conditions (Pushkin) depending on the year (Additional file 3). The remarkable difference in flowering time was hardly due to alleles of Ppd genes, since for both Morex and Barke a recessive allele of Ppd-H1 gene was reported, suggesting reduced response to the long day .
Two significant QTLs affecting heading date in Morex/Barke DH population were mapped on 2H and 3H chromosomes (Fig.4) in two different geographical locations. For both mapped QTLs the prolonged heading date was associated with Barke alleles. One of the QTLs was consistently mapped on the long arm of 3H in the position of 132.7 cM in all environments tested. The position of the QTL perfectly co-segregated with the HvGA20ox2 gene-specific CAPS marker on Morex/Barke genetic map. The maximal proportion of variance (R 2) explained by the QTL varied from 28% to 41% in two geographical locations. DHLs, inheriting sdw1/denso allele from Barke, initiated flowering 3–5 days later than DHLs carrying the HvGA20ox2 allele from Morex.
The closest SNP marker to the QTL on 3H in all five environments was SNP 1_0754; this SNP was co-segregating with BOPA SNP marker 11_0977 in OWB barley genetic map . The SNP 11_0977, in turn, was mapped in the same position as a major QTL peak for heading date and plant height detected on 3H (139 cM) in SBCC145 x Beatrix DH population and was proposed to be the closest marker to the sdw1/denso dwarfing gene .
Variation of thousand grain weight (TGW) and grain quality traits in Morex/Barke DHLs population depending on 7-bp deletion in exon 1 of HvGA20ox2 gene
TGW variation was estimated in the Morex/Barke DHLs population in three environments: Pushkin_2011, Pushkin_2013, Krasnodar_2011. The robust significant QTL peak for the trait was consistently mapped on 2H chromosome (77.6 cM) (Fig. 5), co-segregating with SNP 3_0896, which was reported as POPA SNP marker of the VRS1 locus controlling the inflorescence type . The allelic state of at the VRS1 locus differentiates the wild type two-rowed barley and six-rowed barley. Loss-of-function mutations in the HvHOX1 gene that underlies the VRS1 locus cause a cessation of suppression of lateral-spikelet development and thus lead to the recessive six-rowed phenotype . In the Morex/Barke cross the two-rowed parent Barke carries the dominant Vrs1.b3 allele, while six-rowed parent Morex has the recessive vrs1.a1 allele . Polymorphism in the VRS1 locus explained up to 48% of the TGW variation observed among the DHLs derived from the cross between Morex and Barke (Additional file 4). The two-rowed DHL offspring showed higher TGW values compared to six-rowed DHLs. However, no impact of the sdw1/denso locus on the trait variation was detected.
Grain protein concentration (GPC) is a primary determinant of grain value and malting quality in barley. While very low (< 90 g kg−1) grain protein concentration reduces the value of malting barley, high grain protein concentration (> 145 g kg−1) is associated with low levels of malt extract and increased likelihood of chill haze in finished beer . Since grain protein concentration is impacted both by environmental conditions and genetic background, it was suggested as an ideal subject for QTL analysis and potential marker assisted selection. Starch content affects water uptake during steeping of barley and is considered as the important trait for malting industry . To check whether sdw1.d allele affects GPC and starch content the two traits were assessed in Morex/Barke DHLs populations in five and three environments respectively. Alike to TGW analysis results, the 7-bp deletion in exon 1 of HvGA20ox2 gene did not affected accumulation of protein and starch in grains, while significant influence of VRS1 locus on GPC in Morex/Barke DHLs population was discovered in three out of five environments tested (Additional file 4).
In the present study we investigated effect of sdw1/denso allele on the variation of yield-related and malting quality traits in the population of DHLs derived from cross of medium tall barley Morex and semi-dwarf barley Barke. The latter belongs to the ‘Diamant family’ of the barley varieties inheriting the sdw1/denso allele from X-ray mutant cv. Diamant. The sdw1/denso allele has been reported for more than 150 new successful malting barley cultivars in Europe  and also has gained great acceptance in malting barley breeding programs around the world. The question was whether only the short stature of the sdw1/denso mutants and their resistance to lodging was the cause for the apparent agronomical success of semi-dwarfing barleys, or the pleiotropic effect of sdw1/denso allele on the yield-related traits has played the decisive role. For wheat it was previously reported that the direct effect of the Rht mutations is reduced plant height, but there is also an important pleiotropic effect causing increased assimilate partitioning to developing ears and an increased number of grains per spike and resulting in enhanced yield (reviewed in ). On other hand, the agronomic success of sd-1 mutants in rice may be intimately linked to the developmental timing of stem growth and panicle development .
The sdw1/denso locus was mapped on 3H chromosome of barley few decades ago , since that time many QTL mapping studies reported the linkage of the sdw1/denso locus to agronomical important traits (reviewed in ). However, the candidate HvGA20ox2 gene for sdw1/denso locus and the functional polymorphism assigned to the semi-dwarf alleles were defined just recently [5, 8]. The sdw1/denso allele is most likely resulted from the deletion of 7 bp in exon 1 of HvGA20ox2 gene . The deletion leads to the shift of reading frame of HvGA20ox2 gene and may affect dramatically the structure of corresponding protein. HvGA20ox2 encodes GA 20-oxidase that is involved in the final steps of gibberellin biosynthesis. Mutations of the HvGA20ox2 lead to reduced endogenous GAs concentration affecting stem elongation and also flowering time, since GA acts a particularly important developmental switch between vegetative and reproductive development . Corresponding to these reports, we detected the significant association between the 7-bp deletion in exon 1 of HvGA20ox2 gene and the segregation of plant height and heading date in Morex/Barke DHLs population: DHLs inheriting the sdw1/denso allele from Barke were on average 13 cm shorter in height and initiated flowering 3–5 days later than DHLs carrying the intact HvGA20ox2 allele from Morex. This also confirms previous observations that the sdw1 barley mutants show a 10–20 cm reduction of plant height and 3 days heading delay [15, 25]. Besides, Maurer et al.  recently highlighted the important role of sdw1/denso gene in a whole plant’s life cycle: introgression of the wild sdw1 allele into Barke genetic background from wild barley (Hordeum vulgare ssp. spontaneum) increased plant height by up to 12.3 cm, reduced the time required to reach shooting, flowering and maturity by 5.7, 4.3 and 4.0 days, respectively.
While the main characteristics of the sdw1/denso phenotype, such as a reduction of culm internode length, increased lodging resistance and delayed flowering are generally recognized, its effect on yield components or malting quality traits in barley is still questionable. QTLs for heading date, growth habit, yield, development score, plumpness and hectolitre weight were co-located with the sdw1/denso on the long arm of chromosome 3H (e.g. [6, 25]). Both positive and negative effects of sdw1/denso gene on the yield components in barley were reported [9, 22]. For example, DHLs derived from the cross of the barley variety Magnum and the variety Goldmarker (carrying the sdw1/denso gene) showed lower plant grain weights and 50 grain weights . Grain yield of semi-dwarf lines derived from the crosses of semi-dwarf ‘Royal’ parent and eight tall barley lines was not significantly increased in all the cases . The major issue reported for sdw1/denso barley varieties is the decreased thousand grain weight: replacement of sdw1/denso Barke alleles by those introgressed from wild barley increased TGW by up to 4.5 g . On other hand, the sdw1/denso locus had a large positive effect on grain yield in DHLs population derived from the cross of medium tall AC Metcalfe and semi dwarf Baudin, explaining 49% of the variation .
Final grain yield in wheat and barley depends on grain number and grain weight (reviewed in ). The yield components influencing grain number include number of tillers bearing fertile spikes, extension of vegetative as well as reproductive growth, inflorescence architecture, culm hardiness, spike initiation, elongation and branching as well as spikelet formation. In the Morex/Barke segregating population the variation of grain weight (TGW) and grain quality (GPC) were toughly determined by the VRS1 locus, differentiating the two-rowed and six-rowed DHLs progenies. No impact of the sdw1/denso locus on the yield component and grain quality traits was detected.
The ortholog of HvGA20ox2 gene in rice genome, sd1 is recognized as the classic Green Revolution gene. Similar to barley, large deletions in coding part of the corresponding rice gene Os20ox-2 were discovered for rice semi-dwarf cultivars. Some of them (i.e. IR8) brought the Green Revolution to many countries in Asia  and still show the best agronomic performance . In barley, however, no significant positive pleiotropic effect of sdw1/denso gene on the yield and quality components such as TWG or GPC was detected neither in the present study, nor in the most of previous reports. Though, an indirect positive effect of semi dwarfism on agronomic performance in barley could be assumed. Semi-dwarf barleys are more resistant to lodging than tall plants; lodging reduces not only barley yield and grain quality but also affects malt quality since the grain from lodged plants is often lighter in weight and lower in malt extract . Compared to its ancestor cv. Valticky, the sdw1.d mutant cv. Diamant was reported to increase grain yields by 12% . Triumph derived from Diamant is known as the best genetic sources for malting barley in Europe.
The positive or negative effect of sdw1/denso gene on the yield components in barley could also depend on different environments. Two-rowed sdw1/denso spring barleys could be highly advantageous in Western Europe with the long growing season allowing to accumulate additional biomass that supports higher yields. In hot and dry areas, however, the delay in heading date raises a risk that grains filling will occur concurrently with a drought and high summer temperatures which may harm yield performance of denso-carrying spring barley varieties. As a consequence, lateness in heading associated with denso was reported as another issue of barley breeding in Spain .
In our study the 7-bp deletion in HvGA20ox2 gene, which was recently proposed as the functional polymorphism of sdw1/denso locus in barley, was significantly associated with reduced plant height and delayed flowering time in the segregating population of DHLs derived from cross of medium tall barley Morex and semi-dwarf sdw1.d (sdw1/denso) variety Barke, independently on environmental cue. On other hand, the sdw1.d mutation did not affect either thousand grain weight or grain quality traits variation in this DHLs population. Thus, the beneficial effect of the semi dwarf sdw1.d allele in barley seems does not relate directly to the grain yield potential, but is associated with lodging resistance. Besides, in certain ecological environments the extended period of vegetative growth allows to accumulate additional biomass supporting higher grain yield.
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We are grateful to Prof. Andreas Graner and Prof. Nils Stein for providing the seed material of Morex/Barke DHLs population.
This work was supported by Interstate Targeted Program of Eurasian Economic Community “Innovative Biotechnologies” (grant no. 2014–14-М.04–0008). ML was supported by scientific project no. 37.1521.2014/K of the Ministry of Science and Education of the Russian Federation. The publication costs were funded by the Saint Petersburg State University.
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This article has been published as part of BMC Plant Biology Volume 17 Supplement 1, 2017: Selected articles from PlantGen 2017. The full contents of the supplement are available online at https://bmcplantbiol.biomedcentral.com/articles/supplements/volume-17-supplement-1.
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Mapping of the HvGA20ox2 gene in the Morex/Barke DHLs population on the long arm of 3H chromosome and phenotypic trait data scored for DHLs of the Morex/Barke barley mapping population. (XLSX 27 kb)
Plant height segregation evaluated for 10 individual plants per each of 90 DHLs and two parents the Morex/Barke DHLs population. (XLSX 15 kb)
Flowering time (days to awn emergence) of 90 DHLs derived from Barke and Morex cross in 2011–2013 in two geographical locations: Pushkin (59° 53′ 39″ N) and Krasnodar (45°02′55″ N). (PPTX 72 kb)
LOD scans, additive effect and percent of explained phenotypic variation (R2) of Composite Interval Mapping (CIM) performed for agronomic traits scored for DHLs of Morex/Barke barley mapping population. Obtained from output of Windows QTL Cartographer version 2.5 via Perl script. (XLSX 284 kb)
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Teplyakova, S., Lebedeva, M., Ivanova, N. et al. Impact of the 7-bp deletion in HvGA20ox2 gene on agronomic important traits in barley (Hordeum vulgare L.). BMC Plant Biol 17, 181 (2017) doi:10.1186/s12870-017-1121-4
- Semi-dwarf barley
- Functional polymorphism
- Heading date
- Plant height
- Grain protein content
- Thousand grain weight