Specificity versus redundancy in the RAP2.4 transcription factor family of Arabidopsis thaliana: transcriptional regulation of genes for chloroplast peroxidases
© The Author(s). 2017
Received: 18 February 2017
Accepted: 14 August 2017
Published: 23 August 2017
The Arabidopsis ERFIb / RAP2.4 transcription factor family consists of eight members with highly conserved DNA binding domains. Selected members have been characterized individually, but a systematic comparison is pending. The redox-sensitive transcription factor RAP2.4a mediates chloroplast-to-nucleus redox signaling and controls induction of the three most prominent chloroplast peroxidases, namely 2-Cys peroxiredoxin A (2CPA) and thylakoid- and stromal ascorbate peroxidase (tAPx and sAPx).
To test the specificity and redundancy of RAP2.4 transcription factors in the regulation of genes for chloroplast peroxidases, we compared the DNA-binding sites of the transcription factors in tertiary structure models, analyzed transcription factor and target gene regulation by qRT-PCR in RAP2.4, 2-Cys peroxiredoxin and ascorbate peroxidase T-DNA insertion lines and RAP2.4 overexpressing lines of Arabidopsis thaliana and performed promoter binding studies.
All RAP2.4 proteins bound the tAPx promoter, but only the four RAP2.4 proteins with identical DNA contact sites, namely RAP2.4a, RAP2.4b, RAP2.4d and RAP2.4h, interacted stably with the redox-sensitive part of the 2CPA promoter. Gene expression analysis in RAP2.4 knockout lines revealed that RAP2.4a is the only one supporting 2CPA and chloroplast APx expression. Rap2.4h binds to the same promoter region as Rap2.4a and antagonizes 2CPA expression. Like the other six RAP2.4 proteins, Rap2.4 h promotes APx mRNA accumulation. Chloroplast ROS signals induced RAP2.4b and RAP2.4d expression, but these two transcription factor genes are (in contrast to RAP2.4a) insensitive to low 2CP availability, and their expression decreased in APx knockout lines. RAP2.4e and RAP2.4f gradually responded to chloroplast APx availability and activated specifically APx expression. These transcription factors bound, like RAP2.4c and RAP2.4g, the tAPx promoter, but hardly the 2CPA promoter.
The RAP2.4 transcription factors form an environmentally and developmentally regulated transcription factor network, in which the various members affect the expression intensity of the others. Within the transcription factor family, RAP2.4a has a unique function as a general transcriptional activator of chloroplast peroxidase activity. The other RAP2.4 proteins mediate the fine-control and adjust the relative availability of 2CPA, sAPx and tAPx.
Plants evolved signaling pathways and regulatory networks to sense environmental changes, to process them and to adjust metabolism and growth. In the regulatory circuitries, transcription factors earn a crucial role . The RAP2 (RELATED TO APETALA-2) transcription factors  are a prominent group, which highly diversified during plant evolution. Compared to 12 genes in the green algae Chlamydomonas reinhardtii, the moss Physcomitrella patens encodes 56, most monocots 30–60 and most dicots 120 to more than 200 RAP2 proteins (data taken from plantTFdb; ). Arabidopsis thaliana has 147 open reading frames for RAP2 proteins . Identification of the first RAP2-binding motif in the promoter of ethylene-inducible genes  gave the RAP2 transcription factor subfamily its alternative name ETHYLENE RESPONSIVE TRANSCRIPTION FACTORS (ERF). The characteristic DNA-binding motif, the AP2-domain [2, 4], is formed by three anti-parallel ß-sheets and one α-helix . The two loops connecting the three β-sheets interact with base pairs in the major groove of the DNA .
Here, we focus on a small subgroup, the ERFIb or RAP2.4 proteins. It consists of eight transcription factors, namely RAP2.4a – RAP2.4h . They share a single, highly conserved AP2-domain. Transcription factors with similar DNA binding domains can compete among each other for binding sites. They can either compensate for each other or block each other in gene regulation [7–9].
Only limited information is available on the RAP2.4 family and the competition potential between the transcription factors. RAP2.4a (At1g36060) was isolated in a screening approach for proteins binding to the redox-box of the 2-Cys peroxiredoxin-A (2CPA) promoter . The transcription factor activates 2CPA expression by binding to the CGCG core of a CE3-like promoter element . 2CPA is a highly abundant chloroplast peroxidase . It provides protection against photooxidative stress [12, 13]. 2CPA transcription activity is under control of redox signals, which correlate with the regeneration efficiency of the photosynthetic electron acceptor NADP+ . RAP2.4a dimerization takes place under slightly oxidizing conditions and activates 2CPA transcription. Oligomerization upon severe redox-imbalances inactivates RAP2.4a . Interaction of RAP2.4a with RCD1 (RADICAL-INDUCED CELL DEATH 1) supports activation of 2CPA and other genes for chloroplast antioxidant enzymes, such as thylakoid-bound ascorbate peroxidase (At1g77490; tAPx) and CuZn-superoxide dismutase-2 (At2g28190; Csd2), in young leaves and protects mesophyll cells from early cell death [10, 15, 16]. Overexpression of RAP2.4a (alternatively designated WIND3 (WOUND INDUCED DEDIFFERENTIATION 3; ) under control of the Cauliflower Mosaic Virus 35S (CaMV35S) promoter enables wound-induced cell dedifferentiation via ARR (ARABIDOPSIS RESPONSE REGULATOR)-mediated regulation of cytokinin signaling . Zhu et al.  also reported higher drought tolerance, activation of aquaporins, growth retardation, reduced leaf expansion, transluced rosette leaves in RAP2.4a over-expressing plants. RAP2.4a knockout lines (KO-lines) develop symptomless under non-stress growth conditions, but get chlorotic under naturally fluctuating light conditions .
RAP2.4b, which is induced in RAP2.4a KO-lines , binds the ethylene-responsive GCC-box and the dehydration-responsive element (DRE) . The RAP2.4b transcript level is (in contrast to RAP2.4a ) down-regulated by light, but induced by salt and drought stress. Unlike RAP2.4a , the transcription factor promotes tolerance to salt and drought stress and inhibits ethylene-mediated apical hook formation and hypocotyl elongation . Interaction with BPM (BTB/POZ-MATH) proteins, which are substrate adapters in cullin-E3 ligase complexes, regulates ubiquitination-mediated RAP2.4b degradation . Similar to overexpression of RAP2.4a, over-expression of RAP2.4b (WIND1; At1g78080), and also of presumably post-translationally chloroplast-targeted RAP2.4d (WIND2; At1g22190)  and RAP2.4e (WIND4; At5g65130), but not RAP2.4f (At4g39780) and RAP2.4c (At2g22200), support wounding-induced cell-dedifferentiation . For RAP2.4f (At4g39780) and RAP2.4g (At1g64380), transcriptome analysis showed regulation by pathogens, such as Bortrytis spec. or the plant defense regulator chitin . The responses demonstrate overlapping, but also specific functions of the RAP2.4 transcription factors.
To test the ERFIb / RAP2.4 transcription factors systematically for redundancy and specificity, we analyzed RAP2.4 regulation in T-DNA insertion lines and transient overexpression lines, performed promoter binding studies in yeast and analyzed target gene regulation in Arabidopsis thaliana.
Expression of RAP2.4 transcription factors is hardly regulated throughout development, but responds differentially to stress
To analyze the stress responsiveness, we performed qRT-PCR (quantitative amplification of cDNA by polymerase chain reaction after reverse transcription) analysis for all eight RAP2.4 transcription factors. Wildtype plants were cultivated for 2 weeks at 20 °C and then shifted either for 1 week to 10 °C or 30 °C or for 2 h to excess light (ca. 1000 μmol photons m−2 s−1). Control plants were kept at 20 °C and standard light conditions. The transcript levels of RAP2.4a, RAP2.4c, RAP2.4g and RAP2.4h decreased at 10 °C and that of RAP2.4e increased (Fig. 1b left). The mRNA levels of RAP2.4b, RAP2.4d and RAP2.4f did not change significantly, as compared to 20 °C (two-way ANOVA; p ≤ 0.01). At 30 °C, the transcript levels of RAP2.4a, RAP2.4b, RAP2.4d and RAP2.4g were higher and that of RAP2.4c and RAP2.4h lower than at 20 °C. RAP2.4e and RAP2.4f mRNA levels were barely changed. Similar to the 10 °C treatment, the RAP2.4a, RAP2.4g and RAP2.4h transcript levels decreased in response to the excess light treatment (Fig. 1b right). RAP2.4b, RAP2.4d and RAP2.4f transcript levels increased and RAP2.4c and RAP2.4e mRNA levels were not significantly changed. The experiment showed widely gene-specific regulation.
To test the hypothesis on widely gene-specific regulation on a general basis, we performed transcript abundance correlation analysis in the full abiotic stress data set of Affimetrix chip experiments provided by Genevestigator . The highest Spearman correlation coefficient between two RAP2.4 transcription factor genes was calculated for RAP2.4b and RAP2.4d. It was with 0.44 low (p-value 4.05 × 10−4). The next ranked pair, RAP2.4d and RAP2.4f, gave a Spearman correlation coefficient for transcript abundance regulation of already only 0.3. The p-value for the correlation was higher than 0.01 (1.91 × 10−2) reflecting that the correlation is not significant anymore and supporting the conclusion, that the eight RAP2.4 genes are highly differentially regulated.
Isolation and basic characterization of RAP2.4 T-DNA insertion lines
Impact of the transcription factors on target gene regulation
Consistent with our previous analysis , the transcript levels of 2CPA, sAPx and tAPx were decreased in 3-week-old RAP2.4a KO-lines (Fig. 5). On the contrary, all other RAP2.4 KO-lines had higher 2CPA mRNA levels (Fig. 5). tAPx transcript levels were decreased in all eight RAP2.4 KO-lines and sAPx transcript levels were less abundant than in wildtype plants in all RAP2.4 KO-lines, except RAP2.4c. Lack of RAP2.4b, RAP2.4c, RAP2.4f and RAP2.4g had a stronger effect on tAPx than sAPx, while lack of RAP2.4d and RAP2.4h similarly affected sAPx and tAPx expression. 2CPB, which encodes the second chloroplast-targeted 2-Cys peroxiredoxin besides 2CPA, was slightly stronger expressed in the RAP2.4a KO-line, slightly less in the RAP2.4b, RAP2.4c and RAP2.4d KO-line and not significantly affected in the RAP2.4f, RAP2.4g and RAP2.4h KO-line (Fig. 5).
Yeast-1-hybrid analysis of RAP2.4 binding to the 2CPA and tAPx promoter
Comparison of the structural models of DNA binding domains of RAP2.4 proteins
As compared to RAP2.4a, RAP2.4b, RAP2.4d and RAP2.4h carry substitutions in aa288 (D➔E), aa292 (E➔Q) and aa295 (L➔M), aa299 (R➔K, T) and / or aa302 (Y➔F) in the AP2 domain (Fig. 7). RAP2.4b and RAP2.4d have identical AP2 domains (Fig. 7). The two transcription factors also share the 14 aa long LEKYPSYEIDWDSI sequence (aa451 - aa466). The KYPS and EIDWD motifs were observed in many other ERF transcription factors [4, 30–32] and have been discussed in context of DNA-methylation regulation, gene expression stability control and ethylene responsiveness [4, 30].
RAP2.4f and RAP2.4c carry a R➔K (aa277; blue ring in Fig. 8) substitution in loop 2 between β-sheet 2 and 3, which is in direct contact with the DNA . The amino group of the lysine residue (RAP2.4f and RAP2.4c) is less bulky than the guanidinium group of the arginine residue (RAP2.4a) and has fewer options for H-bridge formation. Protein surface analysis with RasMol  showed the ε-amino group of K277 in close contact with the keto-group of the peptide bond between K277 and N278 (Fig. 8 bottom; blue ring), where it could decrease options for H-bond formation between the transcription factor and the DNA.
In addition to the R➔K substitution in aa277, RAP2.4e has an H➔Q (aa265) substitution in loop 1 between the β-sheets 1 (aa260–264) and 2 (aa267–275), modifying the second DNA contact surface (Fig. 8 mid and bottom). The N-terminus of β-sheets 2 differs in RAP2.4e even stronger from RAP2.4a than the DROUGHT RESPONSE ELEMENT BINDING FACTOR 2A (DREB2A) does (Fig. 8 top). DREB2A belongs to the subfamily IV of the ERF transcription factors  and has a characteristic glutamate residue in the tip of β-sheet 2 (aa275), where the RAP2.4 proteins have an aliphatic leucine residue. In RAP2.4g, the β-sheet 2 and 3 are shortened due to substitutions in aa277 (R➔Q) and aa280 (T➔M) (Fig. 8 top). The sulfur of the M280 site chain is exposed to the DNA contact site and the second guanidinium finger is missing, which otherwise could interact with the negative charges in the DNA backbone (Fig. 8 mid and bottom) pointing out that RAP2.4g has the most severe modification.
Transcription factor regulation network
In all transiently RAP2.4 overexpressing Arabidopsis seedlings (Fig. 10 and Additional file 3), RAP2.4a transcript levels were increased and RAP2.4c transcript levels decreased in response to stronger expression of any transcription factor. The increase in RAP2.4a was accompanied by the induction of RAP2.4b, RAP2.4e and RAP2.4f and a decrease in RAP2.4c and RAP2.4h transcript levels. RAP2.4e and RAP2.4f were stronger expressed in all RAP2.4 over-expressing lines, except the RAP2.4c over-expressors, demonstrating a feedback effect from RAP2.4c on the regulation of these RAP2.4 transcription factors.
Effect of RAP2.4a and RAP2.4h overexpression on 2CPA expression
ROS regulation of RAP2.4 mRNA levels
Regulation in 2CP and APx knock-out lines
ROS bursts in FLU deficient Arabidopsis
As introduced by op den Camp and co-workers , we grew the mutant for 2 weeks in constant light, transferred the plants afterwards for 8 h into darkness and re-illuminated them for 30 and 60 min to induce a rapid release of ROS. The transcript levels of the genes for RAP2.4a, RAP2.4b and RAP2.4d increased in parallel to ZAT10 and BAP1, which are markers for chloroplast ROS signals ; The mRNA levels for RAP2.4g decreased. RAP2.4c and RAP2.4h transcript levels were higher than in wildtype plants after 30 min and decreased after 60 min. RAP2.4e and RAP2.4f transcript levels were only weakly regulated.
The RAP2.4 transcription factors show promiscuity and specificity in binding and function
All eight RAP2.4 genes are actively expressed in Arabidopsis leaves (Figs. 1, 9 and 10), but are differentially regulated in response to environmental variations (Fig. 1b) and share little homology besides the DNA binding domain (Fig. 7). In transcription factor families with conserved DNA-binding motifs, the individual members can have redundant function or act as competitors for DNA-binding sites. For example, the basic helix-loop-helix transcription factors MYC2, MYC3 and MYC4 mediate the jasmonate response in Arabidopsis. Their function is antagonized by bHLH003 bHLH013 and bHLH017, which bind to the same promoter elements with their basic helix-loop-helix motifs, but lack the appropriate gene activating activity .
Binding of all eight RAP2.4 transcription factors to the tAPx promoter in the high protein complexity of yeast cells (Fig. 6) and co-induction of tAPx expression in Arabidopsis (Fig. 5) showed redundancy of the RAP2.4 transcription factors with respect to tAPx regulation. All RAP2.4 transcription factors, except RAP2.4c, also supported sAPx expression significantly (Fig. 5). On the contrary, only the RAP2.4 transcription factors with the most conserved DNA-binding domains, namely RAP2.4a, RAP2.4d and RAP2.4h (and to a lesser extent RAP2.4b), bound the redox-box of the 2CPA promoter (Fig. 6). In this subgroup, only RAP2.4a supported 2CPA expression (Fig. 5). The other RAP2.4 proteins negatively regulated 2CPA expression (Fig. 5 and for RAP2.4h also Fig. 11). Transcript abundance analysis in T-DNA insertion lines (Fig. 9) showed that RAP2.4b and RAP2.4d, in contrast to RAP2.4h, inversely regulate RAP2.4a expression. Consequently, stronger 2CPA expression in RAP2.4b–KO and RAP2.4d–KO might not result from lack of transcriptional inhibition, but could (partly) result also from higher RAP2.4a availability. On the contrary, RAP2.4h suppresses 2CPA promoter activity (Fig. 11) without a significant effect on RAP2.4a transcript levels (Fig. 9) indicating an antagonistic function. As compared to RAP2.4a, RAP2.4h lacks a protein domain with positively charged and hydroxylated amino acid residues (Fig. 7 aa361 – aa368 “charged 3”) and has a two amino acid long insertion in the EIDWD-motif (Fig. 7 aa459-aa460; charged 5) [4, 30–32, 42]. Based on reporter gene studies (Fig. 11) and transcript abundance analysis (Figs. 5, 9 and 10) we conclude that RAP2.4h serves as a transcription inhibitor, whose function is antagonized by RAP2.4a.
In addition to 2CPA promoter regulation, RAP2.4 proteins show specificity in mediating wounding-induced cell dedifferentiation . Overexpression of RAP2.4b (WIND1) and RAP2.4d (WIND2), but also RAP2.4e, have similar effects as overexpression of RAP2.4a (WIND3) . The DNA contact site of RAP2.4e slightly differs from that of RAP2.4a as H265 is replaced by a Q residue next to the DNA-binding stabilizing RQR-motif (Fig. 7) . RAP2.4c, RAP2.4f and RAP2.4g did not bind the 2CPA promoter and also did not induce the WIND-effect , indicating similarities in the transcription factor specificity. RAP2.4h, which is an antagonist of RAP2.4a regulation of the 2CPA promoter (Fig. 11), was not tested on wounding-induced cell dedifferentiation . However, parallelism of RAP2.4a, RAP2.4b and RAP2.4d in the regulation of cell dedifferentiation and of sAPx and tAPx expression and inverse effects of RAP2.4a and the other RAP2.4 proteins on regulation of the genes for chloroplast peroxidases, demonstrate that the specificity and redundancy of the transcription factors depends on the promoters and their protein signatures.
Functional diversification of the RAP2.4 transcription factors
Diversification of the RAP2.4 transcription factor family, resulted in the most specific features for RAP2.4b. RAP2.4b can bind the DRE (DROUGHT-RESPONSE ELEMENT)  as well as the GCC-box, which are typically regulated either by DREBs (DROUGHT-RESPONSE ELEMENT BINDING PROTEIN; designated ERFIV by ) or RAP2.5 proteins (ERFVIII according to ). The structure of DREB2A, a well characterized DRE-binding transcription factor [45, 46], differs in the DNA binding loop 2 and the N-terminus of β-sheet 3 from the predicted common structures of RAP2.4b, RAP2.4a, RAP2.4d and RAP2.4h (Fig. 8). Selectively, only the lack of RAP2.4b, but not of RAP2.4a, RAP2.4d and RAP2.4h, disturbed acclimation to 100 mM NaCl (Fig. 4), demonstrating that the DREB-like effect reported by Lin et al.  is specific for RAP2.4b. Co-induction of RAP2.4a and RAP2.4b by ROS (Fig. 13) points out a circuitry, in which drought- (and ethylene) responses and activation of the chloroplast antioxidant system are co-regulated by independent signaling cascades.
Impact of RAP2.4 regulation on the composition of the chloroplast antioxidant system
2CP and APx both detoxify peroxides inside chloroplasts [47, 48]. They can compensate for the lack of each other under non-stress conditions [13, 36, 49], but have different functions during development and upon stress. 2CP, especially 2CPA, is a highly abundant peroxidase with low catalytic activity . It is expressed early during development and can be regenerated by various small redox proteins, like thioredoxins and NTRC (NADPH-dependent thioredoxin reductase C) [11, 49]. APx have higher catalytic activity . They require ascorbate for regeneration , which can be limiting in growing tissues with low carbohydrate availability [52, 53]. sAPx and tAPx originate from early gene duplication . The proteins have similar catalytic activities, but differ in regulation . tAPx is more important in the response to photooxidative stress than sAPx . tAPx is induced in response to a priming cold stimulus. tAPx accumulation serves as a stress memory and controls activation of ROS signaling cascades . On the contrary, sAPx accumulates during cold acclimation . 2CP is less dispensable upon longer lasting stress, e.g. in excess light, than chloroplast APx . RAP2.4a supports 2CPA, sAPx and tAPx expression, the other RAP2.4 proteins maintain tAPx and sAPx expression and antagonize either directly or indirectly 2CPA gene activity (Figs. 5 and 11), demonstrating that they control on the actual composition of the chloroplast peroxidase system.
RAP2.4b and RAP2.4d are co-induced by singlet oxygen (Fig. 13), but are not induced by low 2CP availability, and the transcript levels are decreased in APx KO-lines (Fig. 12), demonstrating that they are ROS-sensitive, but not specifically regulated by 2CP and / or APx availability.
The RAP2.4a antagonist RAP2.4h was even only transiently induced by FLU-dependent ROS bursts (Fig. 13) and not regulated by APx or 2CPA deficiency (Fig. 12). RAP2.4h responds to light and temperature variation (Fig. 1b) and controls the intrinsically redox-regulated RAP2.4a–mediated 2CPA activation.
Expression of RAP2.4e and RAP2.4f increased in the APx-KO-lines and decreased in the 2CP KO-lines (Fig. 12), which have higher APx levels [13, 49]. The pattern identifies RAP2.4e and RAP2.4f as candidates for APx steady state control.
Although RAP2.4g supports sAPx and tAPx expression (Fig. 5), its expression was almost inactivated in APx single and double KO-lines (Fig. 12) and decreased in response to a ROS-burst (Fig. 13). Lack of RAP2.4g even more than lack of RAP2.4h decreased RAP2.4a expression (Fig. 9), and overexpression increased it (Fig. 10), showing that RAP2.4g expression is essential for full activation of RAP2.4a and identifying RAP2.4g as a potential upstream regulator of RAP2.4a.
The RAP2.4 transcription factors, even if they share a highly conserved AP2-type DNA-binding domain, have overlapping and specific functions in target gene regulation. RAP2.4a is a general activator of 2CPA and APx expression. Any imbalance in the RAP2.4 pattern, except lack of RAP2.4g and RAP2.4h, induces RAP2.4a expression and supports activation of 2CP and APx expression. Dose-dependent effects of RAP2.4h and RAP2.4g on RAP2.4a identify them as important modulators. RAP2.4h antagonizes RAP2.4a, while RAP2.4g indirectly impacts on 2CPA expression by modulating sAPx and tAPx availability. RAP2.4b and RAP2.4d support long term induction of APx and RAP2.4e and RAP2.4f, which do not or only weakly bind to the 2CPA promoter, are involved in the APx steady state control.
Plant material and growth conditions
Seeds of Arabidopsis thaliana var. Col-0 wildtype plants, of RAP2.4 SALK-  and GABI-Kat lines , of 2CP  and APx T-DNA insertion lines  and a reporter gene line expressing glucuronidase under control of the 2CPA promoter  were stratified for 2 days at 4 °C on Arabidopsis soil [70 volumes “Topferde” (Einheitserde, Sinntal-Altengronau, Germany), 70 volumes “Pikiererde” (Einheitserde, Sinntal-Altengronau, Germany), 25 volumes Perligran Classic (Knauf, Dortmund, Germany) supplemented with 0.5 g L−1 dolomite lime (Deutsche Raiffeisen-Warenzentrale, Frankfurt/Main, Germany)] and afterwards transferred to a growth chamber with a day / night cycle of 10 h light at 20 ± 2 °C and 14 h darkness at 18 ± 2 °C. At an age of 8 days, the seedlings were transferred to individual pots (6 cm diameter) and watered once with 0,5 g L−1 Axoris Insekten-frei (COMPO, Münster, Germany). Illumination with 100–110 μmol photons*m−2*s−1 in 10 h light / 14 h dark cycles was performed with L36 W/840 Lumilux Cool White fluorescent stripes (Osram, Munich, Germany). The relative humidity was adjusted to 60% ± 5%.
Alternatively, Arabidopsis thaliana wild-type plants were grown aseptically on 50% MS plates supplemented with 0.5% sucrose  at 10 h light (100 μmol photons m−2 s−1)/ 14 h dark cycles in a Percival CU41 L4 growth cabinet. Surface sterilization was performed as described in .
The flu1 mutant [40, 41] was grown for 2 weeks on Arabidopsis soil at constant light (100 μmol photons m−2 s−1). Afterwards it was transferred for 8 h into darkness and then re-illuminated with 100 μmol photons m−2 s−1 for 30 or 60 min, respectively.
For the temperature treatments, 2 week old soil grown Arabidopsis plants were shifted to 10, 20 and 30 °C warm climate chambers for 1 week and illuminated with the same light regime as described of the standard growth conditions. To modify chlorophyll biosynthesis and photooxidative stress, one third of the 25-day-old plants grown for chlorophyll level analysis were illuminated 3 days for 4 h (starting 4 h after begin of the day period) with 750 μmol photons m−2 s−1. The plant temperature was kept at 18–20 °C with the help of a heat filtering water-bath and an optimized aeration system. The leaf temperature was controlled with an infrared thermometer during the experiment. For the prolonged dark period, the plants were covered with a light-dense shield for 23 h 1 h after the begin of the day period.
Identification of homozygous KO lines
Genomic DNA was extracted from rosette leaves of 2–4 week-old plants, according to standard procedures and tested for the T-DNA insertions with a T-DNA border primer and primers binding approximately 500 bp up- or downstream of the proposed T-DNA insertion site (Additional file 4: Table S1). The DNA of positive plants was subsequently tested for homozygosity of the insertion in a PCR with only the gene-specific primers (Additional file 4: Table S1). As negative control for the insertion test and a positive control for the homozygosity test, the same PCRs were performed with DNA of Arabidopsis thaliana var. Col-0 wildtype plants. Per T-DNA insertion line, eight plants of the progeny were re-tested with the same PCR protocols.
Transient RAP2.4 over-expressor lines
Genomic DNA was isolated from plant material ground in liquid nitrogen, using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). The full-length cDNA sequences of the eight RAP2.4 transcription factors was amplified from the intron-free genes by PCR with gene-specific primers (Additional file 5: Table S2) and inserted into the TOPO-cloning site of pCR8/GW/TOPO (Invitrogen, Carlsbad, U.S.A.). After amplification in E.coli DH5α, the plasmids were isolated. The cDNAs were transferred into the GATEWAY site of pMDC7  with LR clonase enzyme mix (Invitrogene, Carlsbad, U.S.A.), according to the suppliers instructions. Agrobacteria GV3101  were transformed with the RAP2.4-pMDC7 constructs and cultivated in YEB medium (0.5% (w/v) peptone, 0.1% (w/v) yeast extract, 0.5% (w/v) beef extract, 0.5% (w/v) sucrose, 0.5% (w/v) MgCl2). The strains and the agrobacteria strain GV3101 (pMP90) containing the cDNA for the p90 protein under control of the CaMV35S promoter  were grown at 28 °C up to an OD600 of 0.5. Before transfection, cultures of each RAP2.4 strain was mixed 60:40 with cultures of the p90 strain. After 15 min sedimentation at 3000 rpm at room temperature, the agrobacteria were resuspended in 40 mL activation buffer (10 mM MES-KOH pH 5.6; 10 mM CaCl2, 150 μM acetosyringon). After 1 h incubation at room temperature, 5% (w/v) sucrose and 0.02% (v/v) Silwet L-77 (Lehle Seeds, Texas, U.S.A) were added. Sterile plate cultures of 12 day old Arabidopsis seedlings were flooded with 40 mL of this agrobacteria suspension and six times vacuum infiltrated for 1.5 min. Afterwards, the seedlings were transferred on fresh MS plates containing 100 μg mL−1 cefotaxim and 10 μM estradiol. The plantlets were harvested after 1–2 days and immediately frozen in liquid nitrogen.
The rosette diameters and leaf numbers were determined with ImageJ  on digital images of 4-week-old plants.
Chlorophyll-a fluorescence analysis
The maximum quantum efficiency of PS-II (FV / FM = (FM – F0)/FM; ) was determined with a saturating light flash (1300 μmol photons m−2 s−1) after 30 min dark acclimatisation, using a MINI-PAM fluorimeter (Walz, Effeltrich, Germany).
GUS staining and GUS activity quantification
GUS staining and GUS activity quantification were performed as described in .
Seeds of the KO-lines and of Arabidopsis thaliana var. Col-0 wildtype were stratified and grown on 50% MS-medium supplemented with 0.5% (w/v) sucrose, as described in . Five times six seedlings per line were transferred after 2 days to either fresh 100 mm × 100 mm plates with 50% MS-medium and 0.5% (w/v) sucrose or to plates containing 50% MS-medium, 0.5% (w/v) sucrose and 100 mM NaCl. After 7 days, the root length was analyzed with EZ-Rhizo . The root length was normalized on the mean length of the wildtype seedlings grown on the same plate as the series of KO-lines. On each of the 5 plates, the order of the lines was changed. The root lengths on the NaCl plates were standardized on the means of the respective lines on the control plates.
RNA isolation, cDNA synthesis and qRT-PCR
Total RNA was extracted from rosette leaves using the Universal RNA Purification Kit (EURx). cDNA was synthesized from the mRNA, using the High Capacity Reverse Transcription Kit (Applied Biosystems, Carlsbad, CA), and 10 μM oligo-dT16 primer and quantitative real-time PCR was performed on a CFX96 real-time System (Bio-Rad, Hercules, CA), as described recently . Primers spanning exon-intron borders were designed using QuantPrime  (Additional file 6: Table S3). The primer specificity was tested by analyzing the melting curves. For each specific amplicon, they displayed a single peak. The primer efficiency was determined for each primer pair in qRT-PCR reactions with a series of diluted cDNA samples. The non-template control (NTC) was integrated in all qRT-PCR experiments to ensure the purity of the used buffers and solutions and to control possible primer-dimer formation. All reactions were performed for three biological replicates in triplicates. Transcript levels of analyzed genes were standardized on the ACT7 (ACTIN 7, At5g09810) and the YLS8 (YELLOW LEAF SPECIFIC PROTEIN 8; At5g08290) transcript levels .
For the Yeast-One-Hybrid assay with a 2CPA promoter bait the previously described pONE1-derived construct was used, which expresses HIS3 under the control of GAL1, 10 minimal promoter, if transcription factors bind to the promoter . For tAPx promoter reporter plasmid construct, the promoter was divided into two parts, which overlap 110 bp. tAPx-I (−868 to −227) and tAPx-II (−337 to +41) and the sAPx promoter (−908 to −33) were cloned into the pHIS2 vector (Clontech) upstream of the auxotrophic marker HIS3 and the GAL4 minimal promoter. The yeast strain Y187 was transformed with the bait constructs. Prey constructs were generated by cloning the coding sequences of the RAP2.4 transcription factors into the pACT2 vector downstream of the cDNA for the GAL4 activation. Prior to the interaction analysis on SD media lacking leucine (LEU), tryptophan (TRP) and histidine (HIS), the bait strains were co-transformed with empty pAct2 vectors. On 0–100 mM 3-AT, the constructs were tested for autoactivation and suppression of autoactivation by yeast proteins. Afterwards, the yeast-one-hybrid analysis of the RAP2.4 transcription factors was performed on 3-AT concentrations guaranteeing specificity. Colonies were re-assayed on the same auxotrophic medium for interaction confirmation.
Bioinformatics and protein structure modelling
All sequences were extracted from the TAIR database . The RAP2.4 cDNAs were re-sequenced after cloning them into pCR8/GW/TOPO (Invitrogen, Carlsbad, U.S.A.). Sequence alignments were performed online with CLUSTAL Ω  and MUSCLE . Protein modelling was performed with SWISS-MODEL  and RasMol . For comparison the models were overlaied with DeepView .
Gene expression intensity and transcript abundance co-regulation were analyzed on the Genevestigator platform , using all available data sets.
Chlorophyll levels were determined after extraction of the two largest rosette leaves in carbonate-buffered 80% (v/v) acetone according to .
Statistic test were performed with SPSS 22 and SPSS 23 (ANOVA, Tukey test, p < 0.05 or p < 0.01).
- 2CPA / 2CPB:
2-Cys peroxiredoxin A / B
3-Amino-1,2,4-triazole, an inhibitor of histidine biosynthesis
Analysis of variance
Arabidopsis response regulator
Arabidopsis thaliana accession Columbia-0
CuZn superoxide dismutase-2
Dehydration responsive element
Drought response element binding factor
Ethylene responsive transcription factor
Fluorescent in blue light
Galactose synthesis regulating yeast transcription factor
cDNA encoding yeast imidazoleglycero-phosphate dehydrogenase, an enzyme involved in histidine biosynthesis
Knock-out line / T-DNA insertion line of Arabidopsis thaliana
NADPH-dependent thioredoxin reductase C
Over-expressor line of Arabidopsis thaliana
Polymerase chain reaction
Quantitative amplification of cDNA by polymerase chain reaction after reverse transcription
Related to Apetala-2
Arabidopsis thaliana line with T-DNA insertion in a RAP2.4 gene
Radical-induced cell death 1
Stromal ascorbate peroxidase
Thylakoid-bound ascorbate peroxidase
Wound induced dedifferentiation
Gene codes and accession numbers (AGI codes) of analyzed genes
- 2CPA :
- 2CPB :
- Act7 :
- BAP1 :
- RAP2.4a :
- RAP2.4b :
- RAP2.4c :
- RAP2.4d :
- RAP2.4e :
- RAP2.4f :
- RAP2.4g :
- RAP2.4h :
- sAPX :
- tAPX :
- YLS8 :
- ZAT10 :
We thank Prof. Dr. K. Apel for the flu-mutant, Prof. Dr. Francisco Javier Cejudo for the 2CP KO-Lines and Prof. Dr. S. Kangasjärvi for the APx KO-lines, Andras Bittner and Bela Berking for the temperature-stable high-light set-up and supervising the high-light treatment, and Andreas Prescher and Dr. Joern van Buer for critical reading. The work was supported by the German Research foundation (DFG 2011/4 and CRC973-C4) and the Freie Universität Berlin.
Available of data and materials
All data generated during this study are included in this published article. Further data are available via the Genevestigator web interface .
RR selected and characterized the KO-lines, constructed and analysed the OE-lines, performed the transcript abundance analysis and drafted parts of the manuscript and most of the figures. JTB started the yeast-1-hybrid experiments. ER continued the work started by JTB and performed the chlorophyll analysis, UE analysed the salt tolerance of the RAP2.4 KO-lines; MB initiated and supervised the project and finalized the manuscript and the figures. All authors read and approved the final manuscript.
Ethics approval and consent to participate
Consent for publication
The authors declare that they have no competing interests.
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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