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Table 3 Neutral sugar composition of the fractions released by sequential treatment of AIM

From: Cell wall dynamics during apple development and storage involves hemicellulose modifications and related expressed genes

  Treatment A % NSP B % initial sugar content in NSP
NS NS
Rha Fuc Ara Xyl Man Gal Glc UA Rha Fuc Ara Xyl Man Gal Glc UA
60DAF WS   0.0 0.0 0.2 0.0 0.1 0.2 0.2 0.5   1.3 0.8 1.1 0.4 1.9 1.1 0.7 2.2
PS 0.2 0.0 1.9 0.1 1.0 2.1 1.1 5.0 15.2 2.1 11.2 1.1 22.7 11.2 3.7 22.3
Mannanase 0.0 0.0 0.3 0.0 0.3 0.3 0.4 0.0 2.6 0.0 1.8 0.9 6.2 1.3 1.2 0.0
Xylanase 0.0 0.0 0.2 0.0 0.0 0.1 0.1 0.0 1.3 0.0 1.0 0.9 0.1 0.6 0.3 0.0
Glucanase 0.1 0.2 0.4 0.6 0.3 0.3 5.1 0.0 5.7 18.4 2.1 13.1 7.5 1.8 16.4 0.0
2 M WS 0.0 0.0 0.2 0.0 0.0 0.1 0.1 1.3 1.9 0.0 1.6 0.4 1.4 2.0 0.2 4.5
PS 0.3 0.0 2.3 0.1 0.9 0.6 0.2 4.3 21.1 1.5 15.3 1.4 27.8 8.8 0.5 14.8
Mannanase 0.0 0.0 0.2 0.1 0.2 0.1 0.1 0.2 2.0 1.4 1.1 0.8 5.4 1.2 0.3 0.7
Xylanase 0.1 0.0 0.9 0.5 0.0 0.3 0.2 0.0 5.4 0.0 6.2 6.0 0.0 3.6 0.6 0.0
Glucanase 0.1 0.2 0.4 1.0 0.3 0.5 5.7 0.2 9.1 19.8 2.7 12.9 8.2 6.6 15.8 0.7
  1. Analyses were carried out at 2 time points (60DAF and 2 M: i.e., 2 months of cold storage) on samples released after sequential treatments with water (water-soluble, WS), pectinases (pectinase-soluble, PS), mannanases, xylanase and finally glucanase. The neutral sugars (NS) were measured by GC and the uronic acids (UA) by colorimetry. The results are expressed A) as percentage of initial NSP in the AIM dry weight (% NSP) and B) as a percentage of the initial amount of each sugar in the NSP fraction of AIM. Numbers in bold are the maximum of released sugars among all treatments
  2. Rha, rhamnose, Fuc, fucose, Ara, arabinose, Xyl, xylose, Man, mannose, Gal, galactose, Glc, glucose, UA, uronic acids