Cell wall dynamics during apple development and storage involves hemicellulose modifications and related expressed genes

Table 3 Neutral sugar composition of the fractions released by sequential treatment of AIM

	Treatment	% NSP								% initial sugar content in NSP
		NS								NS
		Rha	Fuc	Ara	Xyl	Man	Gal	Glc	UA	Rha	Fuc	Ara	Xyl	Man	Gal	Glc	UA
60DAF	WS	0.0	0.0	0.2	0.0	0.1	0.2	0.2	0.5	1.3	0.8	1.1	0.4	1.9	1.1	0.7	2.2
	PS	0.2	0.0	1.9	0.1	1.0	2.1	1.1	5.0	15.2	2.1	11.2	1.1	22.7	11.2	3.7	22.3
	Mannanase	0.0	0.0	0.3	0.0	0.3	0.3	0.4	0.0	2.6	0.0	1.8	0.9	6.2	1.3	1.2	0.0
	Xylanase	0.0	0.0	0.2	0.0	0.0	0.1	0.1	0.0	1.3	0.0	1.0	0.9	0.1	0.6	0.3	0.0
	Glucanase	0.1	0.2	0.4	0.6	0.3	0.3	5.1	0.0	5.7	18.4	2.1	13.1	7.5	1.8	16.4	0.0
2 M	WS	0.0	0.0	0.2	0.0	0.0	0.1	0.1	1.3	1.9	0.0	1.6	0.4	1.4	2.0	0.2	4.5
	PS	0.3	0.0	2.3	0.1	0.9	0.6	0.2	4.3	21.1	1.5	15.3	1.4	27.8	8.8	0.5	14.8
	Mannanase	0.0	0.0	0.2	0.1	0.2	0.1	0.1	0.2	2.0	1.4	1.1	0.8	5.4	1.2	0.3	0.7
	Xylanase	0.1	0.0	0.9	0.5	0.0	0.3	0.2	0.0	5.4	0.0	6.2	6.0	0.0	3.6	0.6	0.0
	Glucanase	0.1	0.2	0.4	1.0	0.3	0.5	5.7	0.2	9.1	19.8	2.7	12.9	8.2	6.6	15.8	0.7

Analyses were carried out at 2 time points (60DAF and 2 M: i.e., 2 months of cold storage) on samples released after sequential treatments with water (water-soluble, WS), pectinases (pectinase-soluble, PS), mannanases, xylanase and finally glucanase. The neutral sugars (NS) were measured by GC and the uronic acids (UA) by colorimetry. The results are expressed A) as percentage of initial NSP in the AIM dry weight (% NSP) and B) as a percentage of the initial amount of each sugar in the NSP fraction of AIM. Numbers in bold are the maximum of released sugars among all treatments
Rha, rhamnose, Fuc, fucose, Ara, arabinose, Xyl, xylose, Man, mannose, Gal, galactose, Glc, glucose, UA, uronic acids

ISSN: 1471-2229