Fig. 6
a Immunological detection of ectopically expressed TGA5 or TGA5C186S in the tga2 tga5 tga6 (tga256) background with a TGA-specific antiserum. vc, empty vector control. b Growth phenotypes of the plant lines analysed in (a) on MS plates containing 1 % sucrose and supplemented 50 μM TIBA TIBA/0.025 % DMSO (lower panels) or 0.025 % DMSO (upper panels). Photographs were taken two weeks after germination. c Quantitative RT-PCR analysis of CYP81D11 in independent transgenic lines expressing either TGA5 or TGA5C186S. Four-week-old soil-grown plants of the indicated genotypes were either sprayed with 0.1 mM TIBA/0.05 % DMSO or 0.05 % DMSO (mock). Leaves were harvested for RNA isolation after 10 h of treatment. Relative transcript levels were determined using UBQ5 as a reference gene. The mean values (+/−SE) obtained from four to five individually harvested plants are shown.vc, vector control plants