Fig. 5

a Quantitative RT-PCR analysis of ROXY19 and CYP81D11 in roxy19DS mutant plants. Two-week-old MS plates-grown plants of the indicated genotypes were transferred onto MS plates supplemented with 0.1 mM TIBA/0.05 % DMSO or 0.05 % DMSO (mock). Plants were harvested for RNA isolation after 48 h of treatment. Relative transcript levels were determined using UBQ5 as a reference gene. The mean values (+/−SE) obtained from three to four biological replicates (one replicate corresponds to a pool of 40 to 50 plantlets) are shown. b Growth phenotypes of the plant lines analysed in (a) on MS plates containing 1 % sucrose and 50 μM TIBA/0.025 % DMSO. Photographs were taken two weeks after germination. c Western blot analysis to detect HA-tagged ROXY18. For comparison, a protein extract from 35S:ROXY19 line #8 was loaded on the same blot. d Growth phenotypes of the plant lines analysed in (c) on MS plates containing 1 % sucrose and 50 μM TIBA/0.025 % DMSO. Photographs were taken two weeks after germination