- Methodology article
- Open Access
Novel small molecule modulators of plant growth and development identified by high-content screening with plant pollen
BMC Plant Biologyvolume 16, Article number: 192 (2016)
Small synthetic molecules provide valuable tools to agricultural biotechnology to circumvent the need for genetic engineering and provide unique benefits to modulate plant growth and development.
We developed a method to explore molecular mechanisms of plant growth by high-throughput phenotypic screening of haploid populations of pollen cells. These cells rapidly germinate to develop pollen tubes. Compounds acting as growth inhibitors or stimulators of pollen tube growth are identified in a screen lasting not longer than 8 h high-lighting the potential broad applicability of this assay to prioritize chemicals for future mechanism focused investigations in plants. We identified 65 chemical compounds that influenced pollen development. We demonstrated the usefulness of the identified compounds as promotors or inhibitors of tobacco and Arabidopsis thaliana seed growth. When 7 days old seedlings were grown in the presence of these chemicals twenty two of these compounds caused a reduction in Arabidopsis root length in the range from 4.76 to 49.20 % when compared to controls grown in the absence of the chemicals. Two of the chemicals sharing structural homology with thiazolidines stimulated root growth and increased root length by 129.23 and 119.09 %, respectively. The pollen tube growth stimulating compound (S-02) belongs to benzazepin-type chemicals and increased Arabidopsis root length by 126.24 %.
In this study we demonstrate the usefulness of plant pollen tube based assay for screening small chemical compound libraries for new biologically active compounds. The pollen tubes represent an ultra-rapid screening tool with which even large compound libraries can be analyzed in very short time intervals. The broadly applicable high-throughput protocol is suitable for automated phenotypic screening of germinating pollen resulting in combination with seed germination assays in identification of plant growth inhibitors and stimulators.
The identification of novel physiologically active compounds via phenotypic screening of chemical libraries and their application in functional studies becomes increasingly relevant to plant biology [1–3]. In the majority of studies, either plant cell cultures, or whole seedlings are used in phenotype-based chemical screens  aimed at identifying small molecules, which target processes, such as cell wall biosynthesis , cytoskeleton functions , hormone biosynthesis  and signaling [5, 8], gravitropism , pathogenesis, purine biosynthesis and endomembrane trafficking [10–13]. In some cases, associated gene targets have been identified [14–20]. Despite of these achievements, further progress in plant chemical biology largely depends on to what extent image-based screening pipelines can be improved and applied to increase the spatio-temporal phenotypic resolution of fast growing plant systems, and enable the rapid and sensitive screening of large small molecule libraries [14, 21, 22]. Published screens are typically slow using seedlings germinated from seeds and grown in medium, containing the chemicals of interest .
Pollen grains have several unique features, which make them ideally suited to high-throughput chemical biology screens [21, 23]. Firstly, an ample supply of uniform pollen can be easily obtained from only a few flowering plants. Secondly, pollen germination and growth of pollen tubes are very rapid processes, which can be measured efficiently over time-scales of hours. Thirdly, the complete screening procedure can be performed under non-sterile condition on the laboratory bench. Finally, and most importantly, almost 70 % of all genes of the plant under study are transcribed in developing pollen . We therefore hypothesize that any compound, found to inhibit or stimulate pollen germination and tube growth is likely to affect also other plant processes, such as seed germination, growth or differentiation of roots or shoots.
Our high-throughput phenotypical screen integrates operational details of published small molecule screens in various plants and key considerations, when embarking on such a chemical screen. An overview of the strategy is shown in Fig. 1. In tobacco, floral bud size is a good indicator of pollen developmental stage . In order to verify the applicability of this correlation, we analyzed 4′,6-diamidino-2-phenylindole (DAPI)-stained pollen taken from flower buds of different sizes. Results have shown that freshly opened flowers of approximately 40–45 mm in size, which contained fully mature pollen grains, were optimal for screening experiments (Fig. 2). For efficiency of the screen it was critical to identify the correct stage of pollen development for which tobacco pollen grains were collected by gentle stirring of freshly opened flowers into the Eppendorf tube. Then, pollen grains were re-suspended in liquid germination medium (GVH14) and incubated at room temperature. Germination of pollen took place after 30 min and growing pollen tubes could be analyzed after less than 120 min. Next, pollen culture conditions were optimized. The density and homogenous distribution of pollen grains were found to be the most important factor for obtaining reproducible results by image-based evaluation of pollen phenotypes. Most importantly, in order to obtain good images, pollen grains and their growing pollen tubes had to be well separated with little overlap and interference. We tested different densities ranging from 100 up to 10.000 pollen grains per ml medium and found that 4.000 pollen grains per ml were optimal to obtain a good discrimination of individual pollen tubes with meaningful estimation of tube length along with excellent statistical values (in terms of CV and Z’ factor).
For screening experiments, pollen was suspended in GVH14 medium, then transferred into 384 multi-well plates and compounds added to each well. A primary screen plate acquisition, using tobacco pollen germination and tube growth as phenotype features was started after 120 min of pollen incubation with test compounds at room temperature and completed in 8 h. For image analysis we developed an algorithm, which measures the total area, occupied with all visualized pollen grains in each well of multi-well plate (Fig. 3). The quantification of the total area, occupied by all visualized pollen grains, correlates well with inhibitory (total area decreases) or stimulatory (total area increases) effects of tested compounds.
Robustness and reproducibility of the assay was estimated by statistical analysis of the obtained numerical data, collected after a series of at least four identical experiments. Pollen in GVH14 medium containing 0.1 % dimethyl sulfoxide (DMSO) without added chemical compounds was used as a zero control, whereas pollen suspended in GVH14 media containing 0.1 % DMSO, 1 μM salicylic acid, an established inhibitor of pollen germination and tube growth, was used as negative control. For evaluation of assay performance different statistical parameters were used for quality and reproducibility assessment. We determined intra-plate and inter-plate variability, evaluating the data of maximum and minimum signals of control plates, which were set up in duplicates. We also applied Z’-factor analysis with Z-factors typically ranging in average to 0.45 in four independent experiments with +/− 5 % of variability between each experiments depending on different pollen populations chosen for each test. Statistical analysis confirmed reproducible high-throughput screening (HTS) data with standard deviation (SD) 0.45 ± 0.0276 indicating a good dynamic range and high reproducibility (Additional file 1).
Ideally, a chemical library should contain a range of diverse biologically active and structurally diverse chemicals. We first established a focused small chemical library sharing structural features with well-known plant growth inhibitors or activators from indole-, adenine-, β-carboline- and phytodisteroide families . Chemicals were chosen using topological pharmacophore fingerprint, in which Tanimoto coefficient was calculated with respect to the compounds of the reference sample. The final picking included compounds with the highest ratio of topological similarity with respect to known inhibitors and activators of plant growth. In order to evaluate our assay specificity and possible hit rate, the focused small library was randomly incorporated into a panel of 940 compounds chosen via scaffold hopping and similarity of molecular weight, solubility and structure elements. The combined chemical library consisted of 1040 compounds (Additional file 2). Each compound was tested at 10 uM final concentration diluted from 10 mM stock solution dissolved in 100 % DMSO. The primary screening of 1040 compounds resulted in 65 potential hits (Additional file 3), which included inhibitors and stimulators of pollen tube growth.
Compounds, selected from primary pollen screen were further evaluated in secondary screen of Arabidopsis seed germination in order to validate their effects on general plant development. Arabidopsis seeds were germinated in the presence of selected compounds essentially, as described earlier . Nineteen compounds, that were found to inhibit pollen germination and tube growth, inhibited also seed germination and root growth, whereas three compounds, found as stimulators of pollen germination and tube growth, had a similar effect in seed germination and tube growth (Fig. 4, Table 1). These growth inhibitors belong to at least ten diverse chemical classes, including pyrazole, pyrazine, thiourea, thioamide, oxazole, indoline, diazinane, thiazolidine, guaniline and benzazepin (Additional file 4) of 22 compounds selected after primary and secondary screens of 1040 chemicals from Chemical Diversity Research Institute (CDRI) library of chemical compounds). The compounds also cause a reduction in Arabidopsis root length (as % of control) in the range from 4.76 % (I-01) to 49.20 % (I-19), when 7 days old seedlings were grown in the presence of these chemicals. Two chemicals sharing structural homology with thiazolidines (S-01, S-03) stimulated root growth length (as % of control) up to 129.23 and 119.09 %, respectively. The other root growth stimulating compound (S-02) belongs to benzazepin-type chemicals and increased Arabidopsis root length up to 126.24 % as compared to untreated roots (Additional file 4, Fig. 5). We assume that pollen and seed germination are quite similar processes based on common genes and regulatory pathways (Table 1). Results for selected 22 chemical compound hits showed either inhibitory (I) or stimulatory (S) effects in pollen tube and root growth assays. Although germination of seeds occurred after 3 days of culture on agar plates, the final results on seed germination and root growth were obtained only after at least 10–14 days of incubation, thus demonstrating clearly the advantage of pollen system as fast screening system, which can be completed in 2–3 h.
We have shown that a small chemical screen using pollen harvested from few flowering plants is not only one of the fastest systems for HTS, but also versatile as pollen from virtually any plant can be used. This broadens the screening space by linking biological diversity with the available chemical space. The success of the screen depends on the quality of the pollen grains to readily germinate under experimental conditions.
An optimization phase may be necessary for different plant species, but should take only a short time and effort to obtain satisfying conditions based on statistical analysis and enable robust, sensitive, reliable and reproducible screens. Rescreening of hits in different conditions and biological context such as germinating roots from seeds of same plants can be completed in less than 2 weeks. Our study demonstrates that broad versatility of the screen in which compounds either stimulating or inhibiting pollen tube growth are also effective in seed germination and root growth assays thereby suggesting shared targets in growth regulating pathways.
In this study, we demonstrated the usefulness of plant pollen tubes for screening small chemical compound libraries for new biologically active compounds. The pollen tubes represent an ultra-rapid screening tool with which even large compound libraries can be analyzed in very short time intervals. The broadly applicable high-throughput protocol is suitable for automated phenotypic screening of germinating pollen resulting in combination with seed germination assays in identification of plant growth inhibitors and stimulators.
Nicotiana tabacum L. cv. SR1 seeds were kindly provided by Prof. A. Bachmair (University of Vienna, Austria). Plants were grown in greenhouse under long-day photoperiod (16 h-light/8 h-dark, 24 °C) with an illumination of 100–150 μE m−2 s−1 and 60–70 % relative humidity for 6–8 weeks with regular supply of fertilizers and routine watering until intensive flowering and pollen dispersal. Continuous flowering can be achieved by regular harvest of open flowers. Determination of pollen developmental stage was performed by DAPI (Invitrogen, cat. no. D1306) staining (DAPI stock solution: 5 mg DAPI in 1 ml 50 % ethanol). Flower buds at different developmental stages were collected, anthers were dissected from flower buds and placed on a glass slide in a drop of DAPI working solution (DAPI working solution: dilute the DAPI stock solution 1:2000 in 1x Pollen Isolation Buffer (PIB buffer). Anthers were squashed gently by the forceps on the slide to release pollen grains and the slide was covered with a glass coverslip. Preparations were incubated incubate for 5 min at room temperature and observed under a fluorescence microscope using a DAPI filter set.
Pollen collection and preparation
Pollen grains were removed from anthers of open flowers using gentle shaking and collected into centrifuge tubes (volume 50 ml) containing GVH14  medium (708 mg of Ca (NO3)2 x 4H2O, 100 mg of KNO3, 200 mg MgSO4 x 7H2O, 14 mg H2BO3, 1 g of Casein hydrolysate, 10 g of sucrose, and 0.5 g of 2-(N-morpholino) ethansulfonic acid (MES) dissolved in 1 l distilled water, pH = 5.9, filter sterilized). Pollen suspension was diluted to a density of 4.000 pollen grains per ml. At least 30–35 flowers were used for one experiment. Pollen suspension was filtered using the nylon mesh (40 μm) and clean pollen suspension was collected in another centrifuge tube.
Chemical screening and treatment
Chemicals were generously provided by the Chemical Diversity Research Institute (CDRI, Moscow, Russia). All chemicals were dissolved at 100 μM GVH14 supplemented with 14 mg boric acid, and then dispensed by 100 μl per well using BiomekFXP robot system into 96-well plate as follows: 99 μl of medium GVH and 1 μl of chemical compound from 10 mM stock solution (compound library) were added into each well into the columns from 2 to 11 by using 96-tips of Biomek FXP to reach the 100 μM final concentration of the compound. In some wells 1 μl from 1 mM stock solution of salicylic acid (SA) was added into the wells from A1 to D1 and from E12 to H12 (marked as min in Additional file 5). Pollen suspensions were loaded into 384-well plates to final density of 4.000 pollen grains per ml pollen. This generates five 384-well plates for screening of more than 1.000 compounds. In each well 45 μl pollen suspension were automatically added by robotic system. For screening 20 μl compounds were automatically added from 100 μM stock solution to each well, and plates incubated at room temperature for 2 h, or stored at 4 °C until start of the screening procedure, but not longer than 4 h. For phenotypic screening plates were opened and four images per well were taken in transmitted light using 10x objective in ImageXpress Micro XL (Wide field High Content Screening System (Molecular Devices, USA). In total, 1536 images were taken for one plate. Data analysis was conducted using the algorithm, developed based on Custom Module Editor software with registration of individual pollen square (Molecular Devices, USA). Images were processed to define the objects of interest (pollen grains) based on the intensity of images and size of objects.
Assessment of assay variability
Assay was performed in duplicates and during 3 consecutive days on 384-well plates with pollen adjusted to the maximum signal in control plates (medium GVH14 with 0.1 % DMSO). The mean and standard deviation were calculated for each maximum and minimum signal control plates. The data from two 384-multi-well maximum signal control plates were combined to obtain a mean and SD for the replicates. Finally, the combined data of the maximum and minimum plates were used to calculate the Z-factor.
Seed germination and root growth test
Approximately 20 sterilized seeds were used for germination in Murashige and Skoog medium (MS-medium) (1.7 g of (MS) macro salt mixture, 1 g of (MS) micro salt mixture, 1 ml of 1000x (MS) vitamin stock solution and 0.5 g of MES hydrate and 10 g phytagar in 1 L deionized water, pH = 5.7, sterilize by autoclaving 15 min at 121 °C) in a Petri dish, vernalized for 2 to 3 days at 4 °C in the dark, and then transferred to a plant growth room (21–25 °C, 16 h photoperiod). The primary root length was measured after 10 days of growth. In each case at least 20 seedlings were measured. The experiments were repeated at least twice using different lots of seeds.
Chemcial Diversity Research Institute
2-(N-morpholino) ethanesulfonic acid
Murashige and skoog medium
Pollen isolation buffer
Orr DJ, Barding GA, Tolley CE, Hicks GR, Raikhel NV, Larive CK. 1H NMR Based Metabolomics Methods for Chemical Genomics Experiments. Plant Chemical Genomics: Methods and Protocols. 2014;1:225–39.
Norambuena L, Raikhel NV, Hicks GR. Chemical genomics approaches in plant biology. Plant Systems Biology. 2009:345–354.
Dejonghe W, Russinova E. Target identification strategies in plant chemical biology. Front Plant Sci. 2014;5:352.
Hicks GR, Raikhel NV. Small molecules present large opportunities in plant biology. Annu Rev Plant Biol. 2012;63:261–82.
Debolt S, Brabham C. Chemical genetics to examine cellulose biosynthesis. Front Plant Sci. 2013;3:309.
Peng L, Zhang L, Cheng X, Fan LS, Hao HQ. Disruption of cellulose synthesis by 2, 6‐dichlorobenzonitrile affects the structure of the cytoskeleton and cell wall construction in Arabidopsis. Plant Biol. 2013;15(2):405–14.
Meesters C, Mönig T, Oeljeklaus J, Krahn D, Westfall CS, Hause B, … Kombrink E. A chemical inhibitor of jasmonate signaling targets JAR1 in Arabidopsis thaliana. Nat Chem Biol. 2014;10(10):830–836.
Pieterse CM, Leon-Reyes A, Van der Ent S, Van Wees SC. Networking by small-molecule hormones in plant immunity. Nat Chem Biol. 2009;5(5):308–16.
Mähönen AP, ten Tusscher K, Siligato R, Smetana O, Díaz-Triviño S, Salojärvi J, … Scheres B. PLETHORA gradient formation mechanism separates auxin responses. Nature. 2014;515(7525):125–129.
Norambuena L, Hicks GR, Raikhel NV. The use of chemical genomics to investigate pathways intersecting auxin-dependent responses and endomembrane trafficking in Arabidopsis thaliana. Plant Hormones: Methods and Protocols. 2009:133–143.
Rubilar-Hernández C, Hicks GR, Norambuena L. Chemical Genomics Screening for Biomodulators of Endomembrane System Trafficking. Plant Endosomes: Methods and Protocols. 2014:251–264.
Blackwell HE, Zhao Y. Chemical genetic approaches to plant biology. Plant Physiol. 2003;133(2):448–55.
Robert S, Raikhel NV, Hicks GR. Powerful partners: Arabidopsis and chemical genomics. Arabidopsis Book. 2009:e0109.
Hicks GR, Raikhel NV. Opportunities and challenges in plant chemical biology. Nat Chem Biol. 2009;5(5):268–72.
Dai X, Hayashi KI, Nozaki H, Cheng Y, Zhao Y. Genetic and chemical analyses of the action mechanisms of sirtinol in Arabidopsis. Proc Natl Acad Sci U S A. 2005;102(8):3129–34.
Sohn EJ, Rojas-Pierce M, Pan S, Carter C, Serrano-Mislata A, Madueño F, … Raikhel NV. The shoot meristem identity gene TFL1 is involved in flower development and trafficking to the protein storage vacuole. Proc Natl Acad Sci. 2007;104(47):18801–18806.
Zhao Y, Chow TF, Puckrin RS, Alfred SE, Korir AK, Larive CK, Cutler SR. Chemical genetic interrogation of natural variation uncovers a molecule that is glycoactivated. Nat Chem Biol. 2007;3(11):716–21.
Walsh TA, Bauer T, Neal R, Merlo AO, Schmitzer PR, Hicks GR, … Davies JP. Chemical genetic identification of glutamine phosphoribosylpyrophosphate amidotransferase as the target for a novel bleaching herbicide in Arabidopsis. Plant Physiol. 2007;144(3):1292–1304.
Walsh TA, Neal R, Merlo AO, Honma M, Hicks GR, Wolff K, … Davies JP. Mutations in an auxin receptor homolog AFB5 and in SGT1b confer resistance to synthetic picolinate auxins and not to 2, 4-dichlorophenoxyacetic acid or indole-3-acetic acid in Arabidopsis. Plant Physiol. 2006;142(2): 542–552.
Desprez T, Vernhettes S, Fagard M, Refrégier G, Desnos T, Aletti E, … Höfte H. Resistance against herbicide isoxaben and cellulose deficiency caused by distinct mutations in same cellulose synthase isoform CESA6. Plant Physiol. 2002;128(2):482–490.
Brown MQ, Ung N, Raikhel NV, Hicks GR. High-Throughput Identification of Chemical Endomembrane Cycling Disruptors Utilizing Tobacco Pollen. Plant Chemical Genomics: Methods and Protocols. 2014:111–114.
Wang MW, Hao X, Chen K. Biological screening of natural products and drug innovation in China. Philos Trans R Soc Lond B Biol Sci. 2007;362(1482):1093–105.
Ung N, Brown MQ, Hicks GR, Raikhel NV. An approach to quantify endomembrane dynamics in pollen utilizing bioactive chemicals. Mol Plant. 2013;6(4):1202–13.
Mascarenhas JP. Molecular mechanisms of pollen tube growth and differentiation. Plant Cell. 1993;5(10):1303.
Touraev A, Heberle-Bors E. Microspore embryogenesis and in vitro pollen maturation in tobacco. Plant Cell Culture Protocols. 1999:281–291.
The authors would like to thank Prof. M. Kirpichnikov for useful suggestions.
This work was carried out with the financial support of the Ministry of Education and Science of the Russian Federation (Agreement No. 02.A03.21.0003 dated of August 28, 2013) and Russian Fund for Fundamental Research. KP acknowledges support by Bundesministerium für Bildung und Forschung (BMBF Microsystems FKZ 0316185).
Availability of supporting data
All the supporting data are included as additional files.
AT, AI, KS, KP designed the study; RCN, YN, YM and PV developed the experimental methods; RCN, YN and PV performed the experiments, EM, SL developed the experimental methods, critical review of manuscript; KP, RCN, YM and YN wrote the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
Statistical analysis of the assay data to interpret variability. The mean and standard deviation (SD) are calculated for each maximum (max) and minimum (min) signal plates for pollen assay to obtain the coefficient of variation (% CV) for each plate (intra-plate variability). The data from the maximum and minimum replicate plates are then combined to obtain a new mean and SD. These data are used to calculate the Z-factor (four plates testing) for each day. Finally, all of the data from all the maximum and minimum plates are combined to obtain the mean, SD and interplate variability. (DOCX 16 kb)
Listing 1040 chemical compounds library screened on pollen cells. (DOCX 985 kb)
Listing 65 chemical compounds screened in both assays: pollen germination and root growth. (DOCX 15 kb)
Chemical diversity vs biological activity (root length and root growth (% of zero control plant growth) of 22 compounds selected after primary and secondary screens of 1040 chemicals from CDRI library of chemical compounds. (TIF 7536 kb)
General scheme of dispensing tested compounds and control compounds in 96-well plate. Columns 1 and 12 were used for control compounds. Wells A1:D1, E12:H12 were used as negative controls for the minimum signal (n =8). Wells E1:H1, A12:D12 are used as positive controls for the maximum signal (n = 8). All the remaining wells were used for tested chemicals from compound libraries (columns 2–11; n = 80). Positive controls and tested compounds were re-suspended in medium GVH14 with 0.1 % DMSO. Negative controls were re-suspended in medium GVH14 with 0.1 % DMSO in the presence of 1 μM salicylic acid. (DOCX 72 kb)