Fig. 5

Effect of HRW, SNP, cPTIO, L-NAME and NaN₃ on the expression of cell cycle-related genes in adventitious rooting. The primary root system was removed from hypocotyls of 5-day-old germinated cucumber. Explants of cucumber were incubated with 50 % HRW, 50 μM SNP, 200 μM cPTIO, 30 μM L-NAME or 10 μM NaN₃ alone, or in the combination treatments as indicated for 6 h. Then, CycA (a), CycB (b), CDKA (c) and CDKB (d) expression levels were analyzed by real-time RT-PCR. The expression levels of the genes were presented as values relative to water treatment (Con). Values (means ± SE) are the averages of three independent experiments. Bars with different letters were significantly different in comparison with the control at P < 0.05 according to Duncan’s multiple test