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Fig. 3 | BMC Plant Biology

Fig. 3

From: Expression of B-class MADS-box genes in response to variations in photoperiod is associated with chasmogamous and cleistogamous flower development in Viola philippica

Fig. 3

Molecular characterization of AP3-like MADS-box genes in V. philippica. a The ORFs of the VpTM6 genes and its putative peptides. Gaps are introduced to show indels between the two paralogs. The sequences of VpTM6-1 are presented, and the identical sequences in VpTM6-2 are highlighted using dots. The star indicates the stop codon. The M, I, K, and C domains of the hypothetic VpTM6 proteins are indicated. The M domain is highlighted by solid boxes, and the K domain is indicated by dashed boxes. The region between M and K domains is the I domain, while the C domain is behind the K domain. The deleterious amino acid substitutions were highlighted in bold (for details, see Additional file 2: Table S2). b Neighbor-joining (NJ) tree of AP3-like genes. VpTM6-1 and VpTM6-1 are boxed. The accession numbers of other sequences used are LeTM6 (X60759.1), LeAP3 (DQ674532.1), PhTM6 (AF230704.1), PhDEF (X69946.1), MtTM6 (JN412097.1), MtNMH7 (JN412096.1), AtAP3 (NM115294.5), AmDEF (AB516402.1), NtDEF (X96428.1), PfDEF (KC174703.1), and PfTM6 (KC174704.1). OsMADS16 (AF077760.1) is used as outgroup. The species name is listed before the gene name. The numbers next to the nodes are bootstrap values. Multiple sequence alignment for the NJ tree is shown in Additional file 3: (Figure S1a)

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BMC Plant Biology

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