- Research article
- Open Access
Reduced expression of AtNUP62 nucleoporin gene affects auxin response in Arabidopsis
BMC Plant Biology volume 16, Article number: 2 (2016)
The plant nuclear pore complex has strongly attracted the attention of the scientific community during the past few years, in particular because of its involvement in hormonal and pathogen/symbiotic signalling. In Arabidopsis thaliana, more than 30 nucleoporins have been identified, but only a few of them have been characterized. Among these, AtNUP160, AtNUP96, AtNUP58, and AtTPR have been reported to modulate auxin signalling, since corresponding mutants are suppressors of the auxin resistance conferred by the axr1 (auxin-resistant) mutation. The present work is focused on AtNUP62, which is essential for embryo and plant development. This protein is one of the three nucleoporins (with AtNUP54 and AtNUP58) of the central channel of the nuclear pore complex.
AtNUP62 promoter activity was detected in many organs, and particularly in the embryo sac, young germinating seedlings and at the adult stage in stipules of cauline leaves. The atnup62-1 mutant, harbouring a T-DNA insertion in intron 5, was identified as a knock-down mutant. It displayed developmental phenotypes that suggested defects in auxin transport or responsiveness. Atnup62 mutant plantlets were found to be hypersensitive to auxin, at the cotyledon and root levels. The phenotype of the AtNUP62-GFP overexpressing line further supported the existence of a link between AtNUP62 and auxin signalling. Furthermore, the atnup62 mutation led to an increase in the activity of the DR5 auxin-responsive promoter, and suppressed the auxin-resistant root growth and leaf serration phenotypes of the axr1 mutant.
AtNUP62 appears to be a major negative regulator of auxin signalling. Auxin hypersensitivity of the atnup62 mutant, reminding that of atnup58 (and not observed with other nucleoporin mutants), is in agreement with the reported interaction between AtNUP62 and AtNUP58 proteins, and suggests closely related functions. The effect of AtNUP62 on auxin signalling likely occurs in relation to scaffold proteins of the nuclear pore complex (AtNUP160, AtNUP96 and AtTPR).
The nuclear pore complex (NPC) is a huge multiprotein complex, which controls exchanges of macromolecules (RNAs and proteins) between the nucleus and the cytosol in eukaryotes. It forms a doughnut-shaped, eight-fold symmetry structure composed of nucleoporins assembled in different kinds of complexes, forming a spoke/ring structure arranged around a central channel [1–3]. Whereas macromolecules readily diffuse through the NPC when their molecular weight is lower than ca. 30 kDa , they need to interact with nucleoporin-associated receptors (importins and exportins) to be carried from one side to the other when their molecular weight is larger than 40–50 kDa [4, 5]. The NPC is organized in interconnected subcomplexes with distinct functions. Some nucleoporins form the scaffold of the NPC structure, whereas FG nucleoporins, which display large domains containing multiple Phenylalanine-Glycine repeats, are involved in transport of macromolecules in the tunnel by binding to receptor-cargo complexes .
The plant NPC has recently attracted the interest of the scientific community due to the discovery of its contribution to several signalling pathways and, so far, 30 nucleoporins have been identified in Arabidopsis thaliana . Plant nucleoporins are involved in cell responses to diverse hormonal signals as well as to biotic or abiotic environmental stimuli, such as auxin, symbiosis and pathogen attack [3, 7–9]. Suppressor of Auxin Resistance (sar1 and sar3) Arabidopsis mutants, which were screened as suppressors of axr1 (auxin-resistant 1), happened to be invalidated in nucleoporin genes (Arabidopsis NUP160 and NUP96/MOS3, respectively) . Sar1 sar3 double mutants are deficient in mRNA export. Furthermore, regarding auxin signalling, the sar3 and double sar1 sar3 mutants display altered localization of AXR3/IAA17, which is an auxin response repressor from the AUX/IAA family. In these mutants, AXR3/IAA17 was not confined to the nucleus, as expected, but found throughout the cell, suggesting that AUX/IAA repressors are not properly imported or poorly retained inside the nucleus . A nucleoporin of inner filaments of the nuclear basket, named AtTPR or NUA, is also involved in auxin signaling , and the nua mutant is deficient in mRNA export like sar1 and sar3 [11, 12]. Based on two-hybrid and genetic interactions, a functional relationship has also been suggested between AtNUP58 and hormonal (auxin/gibberellin) and light signalling .
This work is focused on the FG nucleoporin AtNUP62 from Arabidopsis thaliana, which is believed to be the orthologue of yeast Nsp1p and vertebrate Nup62 nucleoporins [6, 14]. It is located in the central channel of nuclear pores, together with AtNUP54 and AtNUP58, which are also FG nucleoporins [6, 15]. AtNUP62 is not homologous to other Arabidopsis FG nucleoporins, and it is the only one harbouring the Nsp1-C domain characteristic of yeast and vertebrate Nup62 . Arabidopsis AtNUP62 co-suppressors and mutants were reported to display a dwarf, early-flowering phenotype suggesting an important role in plant development [14, 15]. Overexpression of AtNUP62 in tobacco leaves causes severe tissue decay in tobacco leaves . A systematic search for embryo-defective mutants also identified two atnup62 T-DNA insertion mutants . In this study, we addressed the role of AtNUP62 from the point of view of auxin response.
Variations of AtNUP62 promoter expression
AtNUP62 is not present on the Arabidopsis microarray chip (https://genevestigator.com/, Arabidopsis EFP browser: http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi) and we have therefore not been able to obtain traditional microarray data. However, there are a few tilling array data (https://genevestigator.com/), indicating that the gene is expressed in flowers, seedlings and juvenile leaves, and to a lesser extent in adult leaves. In order to get more precisely the tissue-specific expression of the gene, transgenic plants expressing an AtNUP62 promoter::GUS fusion were created. The gene contains 8 small introns, all located in the last third of the ORF, at a minimum distance of 1.47 kb from the ATG. This distance, together with the negative IMEter scores of these introns (http://korflab.ucdavis.edu/cgi-bin/web-imeter.pl), make it unlikely that they might have a transcriptional activation function [18, 19]. The activity of the GUS gene under the control of AtNUP62 promoter region allowed detecting a specific expression pattern (Fig. 1). Indeed, in adult plants, GUS staining was generally low, except in stipules at the base of cauline leaves, below flower buds (Fig. 1a and b). In other tissues, it was diffuse and preferably localized in developing organs, such as young leaves (Fig. 1c), flowers (Fig. 1d), and root tips (Fig. 1e). In young plantlets, a low-level expression could be detected in the area of veins at the cotyledon tip (Fig. 1f). At early stages, expression could be found in the embryo sac (Fig. 1g and h). The highest GUS activity was observed during germination (2 days after sowing, before the exit of the plantlet from the seed envelope), at the junction between the hypocotyl and cotyledons (Fig. 1i). At the subcellular level, the AtNUP62-GFP fusion protein was distributed at the nuclear periphery, as expected , with a clear punctuate pattern not previously observed (Fig. 1j). The same AtNUP62-GFP construct allowed detecting a broad fluorescence in the cytosol (Fig. 1k), which might be due to overexpression.
Molecular characterization of the atnup62-1 mutant
The AtNUP62 gene displays a specific organization with two completely different zones. The first zone is intronless, represents the initial two thirds of the ORF and encodes the FG repeat domain of the protein (Fig. 2a). Conversely, the second zone harbours multiple small introns. The encoded sequence displays many acidic and basic amino acids forming the Nsp1-C domain homologous to that found in the yeast Nsp1p nucleoporin.
We tried to isolate homozygous plant lines for two different alleles, SALK_071950 and SALK_037337, both displaying a T-DNA insertion in the 5th intron. We were able to isolate viable homozygous seeds only from SALK_037337 (atnup62-1), in agreement with the previously observed lethal effect of the SALK_071950 (atnup62-3) mutation . The third mutant, SAIL_127_F01 or atnup62-2, displaying a 53 amino acid deletion, has a less visible phenotype compared to atnup62-1  and was not used in this study. RT-PCR (Fig. 2a) and quantitative real-time PCR results (Fig. 2b) indicate that the atnup62-1 mutant is not a bona fide knock-out plant but a mutant with reduced transcript levels. Sequencing PCR products (obtained with primers “B” in Fig. 2a) at the exon 5-exon 6-junction region revealed no difference in sequence between the wild type and the mutant (data not shown). Thus, in the mutant plant, the 5th intron that contains the T-DNA can be spliced exactly as the 5th intron of the wild type gene (at the position indicated by TAIR website, www.arabidopsis.org), albeit with decreased efficiency. Real-time quantitative PCR results (Fig. 2b) allowed concluding that the atnup62 mutant displayed a strong reduction of the spliced transcript.
Developmental defects of the atnup62 T-DNA insertion mutant in reproductive organs and germinating seedlings
Previous analyses of a suppressive mutant  and two T-DNA insertion mutants [13, 15] revealed the same phenotypic defects. All plants impaired in AtNUP62 function were small with reduced leaf blades and bolting occurred earlier than in the wild type.
We examined atnup62-1 plants at different growth stages. In vitro-grown mutant seedlings (MS/2 medium) displayed small cotyledons (Fig. 2c, lower images) and frequent abnormalities such as cotyledon malformations, fused cotyledons and polycotyly (3 or even (rarely) 4 cotyledons; Fig. 2c and Table 1). This change in cotyledon number is exceptional in wild-type Col-0 plants (about 1 ‰). Cotyledons of mutant plantlets also became epinastic (Fig. 2c, second and third bottom pictures). In the greenhouse on compost, at the rosette and subsequent stages, mutant plants had smaller leaf blades than wild type plants and flowering occurred earlier, as described previously [14, 15]. Besides, other anomalies were also detected. Flowers were often abnormal and siliques much smaller than in wild type plants (Fig. 2c), with a longer desiccation time. We harvested 603 ± 15 mg of wild-type seeds per plant vs 270 ± 38 mg of mutant seeds from plants grown in parallel in individual pots. The AtNUP62 cDNA under control of AtNUP62 gene promoter complemented the germination and adult plant phenotypes (Fig. 2b and c) as well as the cotyledon phenotypes.
Interestingly, the 35S::AtNUP62-GFP transformed plant displayed a high level of AtNUP62 transcript (Fig. 2b), a marked phenotype of reduced growth at the adult stage (Fig. 2c) and an intermediate phenotype in cotyledons (no or weak epinasty, but presence of a few plants with three cotyledons) (Table 1).
The atnup62 mutant and the 35S::AtNUP62-GFP transformed plant are hypersensitive to auxin
Auxin sensitivity of wild type and mutant plants was examined by growth on 2,4-dinitrophenoxyacetic acid (2,4-D), a stable auxin analogue. On 100 and 200 nM 2,4-D, aerial parts of wild type and complemented plants were moderately affected by the treatment, which mostly inhibited root growth (Fig. 3a).
Root growth on vertical agar plates was compared at the different concentrations of hormone. Whereas roots of Col, atnup62, complemented and 35S::AtNUP62-GFP transgenic plants were all affected in the presence of 2,4-D, the effect was stronger in the mutant and in the 35S::AtNUP62-GFP genotypes, whose residual primary root growth on 50 nM 2,4-D was only about 21 and 16 % of that on control medium, compared to about 40 % for the other two genotypes (Fig. 3b). A phenotype was also observed for the atnup62 mutant plants in cotyledons. These organs, already reduced and epinastic in the absence of auxin analogue treatment, became tiny and severely distorted on 100 nM 2,4-D (Fig. 3a and Additional file 1: Figure S1). Despite their root phenotype similar to that of the mutant, the 35S::AtNUP62-GFP plantlets did not display the phenotype of small epinastic cotyledons (Fig. 3a). This paradox can be explained by the distribution of the AtNUP62-GFP fusion protein, which appears to be almost absent in cotyledons both on MS/2 and 2,4-D-containing media (Fig. 3c).
The DR5 synthetic promoter is commonly used to detect auxin activity . In a wild type genetic background, GUS expression under the control of DR5 can be detected in root tips, hydathodes and leaf margins (, and Fig. 3d). In cotyledons, intense blue staining was always restricted to leaf margins (Fig. 3d, left). Homozygous atnup62 mutant seedlings harbouring the DR5::GUS construct, compared to isogenic DR5::GUS seedlings, displayed a higher GUS activity in cotyledons, in which the staining extended towards the centre of these organs (Fig. 3d, left). This suggested altered auxin activity in the atnup62 plants. In young germinating seedlings, DR5::GUS activity was restricted to the cotyledons, the root tip and the inner edge of the hypocotyl alongside the cotyledons, in agreement with previous reports [21, 22]. The atnup62 mutation resulted in an extension of the staining to the external upper part of the hypocotyl, where AtNUP62 is expressed (Fig. 3d, right).
AtNUP62 is a suppressor of auxin resistance conferred by the axr1 mutation
The AXR1 (auxin-resistant 1) gene encodes a subunit of the RUB-activating enzyme, necessary for the auxin-dependent degradation of AUX/IAA transcriptional repressors. Axr1 mutants are resistant to auxin and also display a specific phenotype under standard growth conditions. This consists in a reduced height, defect in root gravitropism, abnormal inflorescences, low fertility , and serrated leaves . In contrast to axr1, the atnup62 mutation conferred sensitivity to auxin. We therefore created the atnup62 axr1 double mutant. This mutant had lost the phenotype of leaf serrations (Fig. 4a). Leaves were also frequently embossed and misshaped, suggesting additional developmental problems that were not present in the parent lines (Fig. 4a). Most importantly, the auxin resistance of the axr1 mutant was reversed by the atnup62 mutation. Indeed, axr1 plants had no apparent growth defect on 200 nM 2,4-D whereas double mutant plants were sensitive to the auxin analogue treatment (Fig. 4b, c and Additional file 1: Figure S1). The restoration of root sensitivity was partial on 50 nM 2,4-D but complete on 100 nM (Fig. 4c). Q-PCR analyses also revealed that the double mutant had a less reduced amount of AtNUP62 transcript compared to the atnup62 single mutant (Fig. 4d).
AXR1 mediates auxin response by activating the SCF E3 ubiquitin ligase complex that targets the AUX/IAA repressors of auxin response for ubiquitination and degradation . In the auxin signalling cascade, this SCF complex is composed of SKP1/ASK1, the cullin CUL1 and the TIR1 F-box protein that specifically recognizes the AUX/IAA proteins to be ubiquitinated and degraded by the proteasome. A two-hybrid screening with AtNUP58, one of the other two FG nucleoporins of the central channel of the NPC, resulted in the identification of AtNUP62 (C-terminal Nsp1 domain), SKP1, and one of its homologues (SKP1B) . SKP1 was found 8 times in that screening, always in frame with the activation domain of GAL4, from different positions in its sequence (from the 7th to the 89th amino acid). We therefore tested the hypothesis of an interaction between AtNUP62 and SKP1 (Additional file 2: Figure S2b), in both bait/prey combinations. AtNUP62 displayed a strong intrinsic transcriptional activity, leading to growth on medium lacking histidine (Additional file 2: Figure S2, right). The presence of SKP1 fused to the Gal4 activator domain (full-length clone obtained in a previous two-hybrid screening with another bait) did not increase the signal. In the reciprocal test, SKP1 displayed a weak transcriptional activity, which was not enhanced by AtNUP62 (Additional file 2: Figure S2).
AtNUP62 expression and plant phenotype
AtNUP62 is an essential gene, as demonstrated by the lethality of two out of four T-DNA insertions . The developmental phenotype we observed for the atnup62-1 mutant (anomalies in cotyledon shape and number, small siliques) is in accordance with the localization of AtNUP62 promoter expression in the embryo sac and with the “embryo-defective” phenotype evidenced by the Seedgenes project . Another mutant, atnup62-3, harbouring a T-DNA insertion in the same intron as atnup62-1, displays a more severe “embryo-defective” phenotype , leading to lethality (, this study). The presence of a residual ability to splice the fifth intron in atnup62-1 might explain this phenotypic difference between the two mutants.
It also seems difficult to obtain plants that deeply overexpress the AtNUP62 protein. Zhao and Meier  obtained co-suppressors, but not overexpressors, by a strategy of plant transformation with a 35S::FLAG-AtNUP62 construct. Overexpression of AtNUP62 by inoculation in tobacco leaves leads to very severe necrosis . Despite the high level of AtNUP62-GFP transcript (Fig. 2b), the similitude of the phenotypes of atnup62 and 35S::AtNUP62-GFP lines indicates that the latter are loss-of-function rather than strong gain-of-function lines (Figs. 2c and 3), suggesting a dominant negative effect.
The localization of AtNUP62 expression in the embryo sac and young seedlings (Fig. 1) is consistent with the embryo-defective phenotype, cotyledon and plantlet developmental defects. Interestingly, the AtNUP62 promoter activity is concentrated in tissues that synthesize, accumulate or transport auxin. Cotyledon tips and embryos are specific expression sites for expression of YUCCA genes encoding enzymes in the auxin synthesis pathway . In the root apex  and germinating seedlings [21, 22], the AtNUP62::GUS activity does not match with the DR5-GUS activity, but encompasses zones of active auxin transport (cotyledon veins, root tip and the apical hook of the young seedling). Stipules of rosette leaves have been reported, from the activity of the DR5 auxin-responsive promoter, to accumulate free auxin , and have been postulated to regulate adjacent organ development . Auxin is produced in flower organs, especially stamens , but a role of stipules of cauline leaves in flower development is not excluded.
AtNUP62 and auxin signalling
It has been previously reported that some scaffold nucleoporins or NPC-associated proteins, AtNUP160/SAR1, AtNup96/SAR3 , AtNUP58 , and AtTPR , are involved in auxin responses. All mutants in these genes have in common the partial suppression of the axr1 auxin resistance phenotype. The present results provide several lines of evidence supporting the hypothesis that AtNUP62 also plays a role in auxin signalling. Developmental defects, such as reduced size, small leaves , abnormal flowers  and early flowering  suggested that AtNUP62 could interfere with auxin distribution or signalling. The embryo and cotyledon phenotypes of the atnup62 mutant are reminiscent of those of mdr1 and pin1 mutants impaired in polar auxin transport [33, 34] and yucca mutants impaired in auxin synthesis . Cotyledon epinasty is one of the symptoms of auxin excess [26, 35], also displayed by the atnup58 mutant , whereas the appearance of extra cotyledons is a sign of dysfunction in auxin distribution [36, 37]. Indeed, cotyledons, which are formed at the triangular stage of embryo development (between globular and heart stage), emerge from spots of auxin accumulation at the two side apexes of the triangle . The atnup62 mutation suppresses phenotypic traits of the axr1 mutant, notably the auxin resistance, but also the typical serrated leaf phenotype that is auxin-dependent . Finally, both atnup62 mutant plants and the 35S::AtNUP62-GFP plants are hypersensitive to auxin. This phenotype seems to be rather specific. Indeed, atnup160/sar1 and atnup96/sar3 do not display auxin hypersensitivity . Among nucleoporin gene mutants, only atnup58 was reported to be more sensitive than the wild type . Interestingly, AtNUP58 is the only known nucleoporin that binds to AtNUP62, and reciprocally . Hypersensitivity to auxin was also observed in mutant plants impaired in auxin membrane secretion  or control of AUX/IAA degradation .
An additional phenotype of the atnup160/sar1 and atnup96/sar3 mutants is the accumulation of mRNAs in the nucleus . However the atnup62-1 mutant does not accumulate nuclear mRNAs . The same holds true for atnup62-2 and mutants deficient for genes encoding other nucleoporins of the central channel of the NPC . This suggests that accumulation of mRNAs in the nucleus and auxin-related phenotypes are independent phenomena.
Elucidation of the molecular basis of the relationship between AtNUP62 and auxin signalling will need further investigations. Essential genes are statistically prone to be highly connected in gene networks . AtNUP62 is notably connected to genes encoding nuclear pore proteins, and those involved in embryo development and protein ubiquitination (Aranet server, ). The decrease of AtNUP62 transcript thus probably affects several networks. In order to address the molecular mechanisms affected in nucleoporin mutants, Parry  published a transcriptional analysis of atnup160-4 and atnup62-2. Surprisingly, only a few genes displayed a more than twofold change in gene expression compared to the wild type. Among these, in a list of 18 genes up-regulated in both mutants, five were involved in nuclear transport and two (SAUR9 and ACS4) were auxin-responsive. The identification of other targets would probably require tissue-specific RNAs or sampling at early stages (embryo development and germination).
We propose that AtNUP62 would act as a negative regulator of some auxin responses, like nucleoporins AtNUP160/SAR1 and AtNUP96/SAR3 . The negative role of these two nucleoporins in auxin signalling has been ascribed to the fact that these proteins are involved in the retention of the transcriptional regulator AUX/IAA17 inside the nucleus. The suppression of the axr1 phenotype by the atnup62 mutation suggests that AtNUP62 also acts downstream of AXR1. The mechanisms that could underlie such a control needs to be better resolved, but two-hybrid interaction experiments with AtNUP58  and the absence of evidence for a direct interaction of AtNUP62 with SKP1 (Additional file 2: Figure S2) suggest that AtNUP62 might modulate the activity or nuclear retention of SKP1 in part via its interaction with AtNUP58. This is corroborated by the similitude of plant phenotypes (cotyledon epinasty and root hypersensitivity to auxin). It is not excluded that AtNUP62 might also interfere with SKP1-like proteins (family of 21 members in Arabidopsis) , and/or some target components of the auxin signalling pathways, TIR/AFB receptors (6 members in Arabidopsis), AUX/IAA repressors (29 members) and ARF transcriptional factors (23 members) which form a complex combinatorial network, displaying specific expression patterns and involved in different types of responses .
Our data provide a first explanation for the lethality of knock-out atnup62 mutants, by highlighting its role in auxin-dependent development, especially at early stages. In the auxin signalling pathway, AtNUP62 would act downstream from AXR1. This also suggests that this plant FG nucleoporin, in close connexion with AtNUP58 and in relation to scaffold nuclear pore components (AtNUP160/SAR1, AtNUP96/S, and AtTPR), is able to take part in the control of other SKP1-dependent regulatory pathways, at the time and in the place where regulations are required.
Arabidopsis mutant lines
The atnup62-1 and atnup62-3 mutant lines (Col-0, SALK_037337 and SALK_071950) were obtained from the Nottingham Arabidopsis Stock Center . Homozygotes were selected by PCR for insertion of T-DNA (primers at the T-DNA left border and in the AtNUP62 gene) and for disruption of the AtNUP62 gene (primers on both sides of the insertion site). The axr1 mutant is axr1-3 . For the complementation of the atnup62 mutant, AtNUP62 cDNA was amplified by PCR using oligonucleotides 5′-TTGTAGGTCACCTCAAGACATCCAGTGCTTTGGAGCC-3′ and 5′-ACCCGCCATGGCGGGGTTTCCATTTGGTCAATCC-3′, digested with NcoI and BstEII, and introduced into pCambia vector that had previously incorporated 1.8 kb upstream region from AtNUP62 gene with NcoI site at its 3′ end.
Plant growth conditions
Plants were grown in the greenhouse (8 h/16 h 21 °C /23 °C dark/light, light supplemented if necessary with sodium vapour lamps providing 150 μE.m−2.s−1) or in vitro in growth chamber (16 h-light photoperiod, 140 μM photons.m−2.s−1, 20 °C and 70 % humidity during both light and darkness) on half-strength Murashige and Skoog (MS/2) medium, supplemented when necessary with kanamycin (50 mg.L−1), hygromycin (30 mg.L−1) or 2,4-D.
Localisation of AtNUP62 promoter activity in planta
A 1.8 kb region upstream from the ATG of the AtNUP62 gene (At2g45000) was amplified by PCR, using the 5′-GGTTACATTGTCGTGGTCGAGGTACG-3′ and 5′-CCCCGCCATGGCGGGTTATTGATTG-3′ primers and cloned into pBI-320X (Sal I/Nco I). The amplified promoter region was fused to the β-glucuronidase gene (EcoR I/Sac I fragment) and inserted into the binary vector pMOG406. Arabidopsis thaliana plants (Ws ecotype) were transformed using the floral dip method . Transformants were selected on kanamycin, and homozygotes were recovered at the next generation. Beta-glucuronidase activity was detected according to .
Localisation of AtNUP62 protein in protoplasts and plants by fusion with GFP
AtNUP62 (At2g45000) cDNA was amplified with oligonucleotides 5′-CACCATGTCGGGGTTTCCATTTGGTC-3′ and 5′-AGACATCCAGTGCTTTGGAGCCA-3′ (ORF 5′ end and 3′ end without Stop codon), and cloned into pENTR/D-TOPO (Invitrogen). The cDNA sequence was introduced by LR recombination (Gateway LR clonase enzyme mix, Invitrogen) into pGWB5 (Tsuyoshi Nakagawa, Shimane University, Japan) in order to fuse GFP to the C-terminus of the AtNUP62 protein (resulting in pGWB5-AtNUP62 plasmid used for plant transformation). Col-0 plants were transformed according to . For transient expression in protoplasts, the HinDIII/StuI cassette (including the 35S promoter and GFP-coding sequences) was extracted from pGWB5-AtNUP62 and inserted into pGreen 0179, digested with HinDIII and EcoRV. Fluorescence was detected under a Zeiss confocal microscope (LSM510 AX70 Zeiss, Göttingen, Germany). The excitation was obtained with a Beam splitter HFT 488, and the emitted radiations were selected with BP 505–530 nm filter.
Inflorescences (including flower buds and young siliques) were pooled and ground in liquid nitrogen. RNA and genomic DNA were extracted from the same powder sample. RNA was extracted with RNeasy mini kit (Qiagen). Reverse transcription was achieved with 2.5 μg RNA using Superscript (Invitrogen). Two primer pairs, A and B, were used for PCR. (A): 5′-ACTCCGGCTAGCTCCGCTGCTAC-3′ and 5′-TTAGATCTTCAAGACATCCAGTGCTTTGGAGCC-3′ (STOP primer). (B): 5′-CTAGCTTGGAACGACAGCTGGA-3′ and 5′-TCTCTTTCTACTAGCTCAGACTG-3′. EF1-α was used as a control housekeeping gene, with primers5′-CCACCACTGGTGGTTTTGAGGCTGGTATC-3′ and 5′-CATTGAACCCAACGTTGTCACCTGGAAG-3′. To ensure that no wild-type sample contamination occurred in mutant samples during RNA extraction and subsequent steps, a completely independent repeat of RNA/DNA extractions and RT-/genomic DNA PCRs (primers B) was done and results were identical.
Gene expression analysis
Total RNAs were extracted from plantlets grown on half-strength Murashige and Skoog (MS/2) medium for 8 days using the RNeasy Plant Minikit (Qiagen) and quantified by nanodrop after DNase I treatment (Invitrogen). First-strand cDNAs were synthesized with SuperScript III reverse transcriptase (Invitrogen), according to the manufacturer’s instructions. Oligonucleotides for gene-specific amplification were designed using PRIMER3 software (http://frodo.wi.mit.edu/primer3/). The primer pair AtNUP62-F (5′-GCAGAGTGGGATAAGCGGAT-3′) and reverse primer of pair B (5′- TCTCTTTCTACTAGCTCAGACTG-3′) spans intron IV and V, according to AtNUP62 gene structure (Fig. 2), the 5th intron containing the T-DNA insertion (Fig. 2a). For normalization, the PDF2 reference gene (At1g13320) was selected on the basis of its expression stability in roots and leaves under our conditions (primers PDF2-F (5′-TAACGTGGCCAAAATGATGC-3′) and PDF2-R (5′-GTTCTCCACAACCGCTTGGT-3′)). PCR reactions were performed on a LightCycler 480 (ROCHE Applied Science) in triplicate with two independent biological samples. Absence of genomic DNA and primer dimers was confirmed by analysis of minus-RT and water control samples, and by examination of melting curves. Baseline data were collected between cycles 3 and 15. All amplification plots were analysed with an Rn (normalized reporter) threshold of 0.2 to obtain CT values. Data were analyzed using Roche LightCycler software and to derive relative expression levels, the comparative CT method (DDCT) was used as described in Cuéllar et al. .
Selection of atnup62 mutant plants harbouring the DR5::GUS construct
Atnup62-1 homozygous mutant plants were crossed with plants homozygous for the DR5::GUS insertion . The presence of SALK T-DNA insertion in AtNUP62 gene and DR5::GUS integration were confirmed respectively by PCR and ß-glucuronidase activity, and homozygotes were selected at the next generation by checking the absence of AtNUP62 PCR product and the presence of GUS activity in the progeny (100 % of GUS positive plantlets).
A partial AtNUP62 cDNA encoding the NSP1 C-terminal domain (from S266 to the end of the polypeptide sequence) and the full-length SKP1 cDNA were obtained by a two-hybrid screening of the FL4000AB cDNA library (Clontech). The full-length AtNUP62 cDNA clone was obtained by PCR and inserted into pGBT9  and pGAD10 (Clontech). The BglII fragment of the library SKP1 cDNA clone was inserted into pGBT9 previously digested with BamH1. Yeast transformation (AH109 strain) was performed according to . Yeast transformants were grown in liquid medium lacking tryptophan and leucine, cells were washed with water, and threefold serial dilutions (first dilution corresponding to an OD of 0.066 at 600 nm) were dropped on medium lacking tryptophan, leucine and histidine .
Availability of supporting data
All the supporting data are included as additional files.
Nuclear pore complex
Rout MP, Wente SR. Pores for thought: nuclear pore complex proteins. Trends Cell Biol. 1994;4:357–65.
Marelli M, Dilworth DJ, Wozniak RW, Aitchison JD. The dynamics of karyopherin-mediated nuclear transport. Biochem Cell Biol. 2001;79:603–12.
Xu XM, Meier I. The nuclear pore comes to the fore. Trends Plant Sci. 2008;13:20–7.
Gasiorowski JZ, Dean DA. Mechanisms of nuclear transport and interventions. Adv Drug Deliv Rev. 2003;55:703–16.
Terry LJ, Wente SR. Flexible gates: dynamic topologies and functions for FG nucleoporins in nucleocytoplasmic transport. Eukaryot Cell. 2009;8:1814–27.
Tamura K, Fukao Y, Iwamoto M, Haraguchi T, Hara-Nishimura I. Identification and characterization of nuclear pore complex components in Arabidopsis thaliana. Plant Cell. 2010;22:4084–97.
Chinnusamy V, Gong Z, Zhu JK. Nuclear RNA export and its importance in abiotic stress responses of plants. Curr Top Microbiol Immunol. 2008;326:235–55.
Lee Y, Lee HS, Lee JS, Kim SK, Kim SH. Hormone- and light-regulated nucleocytoplasmic transport in plants: current status. J Exp Bot. 2008;59:3229–45.
Wiermer M, Cheng YT, Imkampe J, Li M, Wang D, Lipka V, et al. Putative members of the Arabidopsis Nup107-160 nuclear pore sub-complex contribute to pathogen defense. Plant J. 2012;70:796–808.
Parry G, Ward S, Cernac A, Dharmasiri S, Estelle M. The Arabidopsis SUPPRESSOR OF AUXIN RESISTANCE proteins are nucleoporins with an important role in hormone signaling and development. Plant Cell. 2006;18:1590–603.
Jacob Y, Mongkolsiriwatana C, Veley KM, Kim SY, Michaels SD. The nuclear pore protein AtTPR is required for RNA homeostasis, flowering time, and auxin signaling. Plant Physiol. 2007;144:1383–90.
Xu XM, Rose A, Muthuswamy S, Jeong SY, Venkatakrishnan S, Zhao Q, et al. NUCLEAR PORE ANCHOR, the Arabidopsis homolog of Tpr/Mlp1/Mlp2/megator, is involved in mRNA export and SUMO homeostasis and affects diverse aspects of plant development. Plant Cell. 2007;19:1537–48.
Ferrández-Ayela A, Alonso-Peral MM, Sánchez-García AB, Micol-Ponce R, Pérez-Pérez JM, Micol JL, et al. Arabidopsis TRANSCURVATA1 encodes NUP58, a component of the nucleopore central channel. PLoS One. 2013;doi: 10.1371/journal.pone.0067661.
Zhao Q, Meier I. Identification and characterization of the Arabidopsis FG-repeat nucleoporin Nup62. Plant Signal Behav. 2011;6:330–4.
Parry G. Components of the Arabidopsis nuclear pore complex play multiple diverse roles in control of plant growth. J Exp Bot. 2014;65:6057–67.
Kemp C, Coleman A, Wells G, Parry G. Overexpressing components of the nuclear transport apparatus causes severe growth symptoms in tobacco leaves. Plant Signal Behav. 2015;doi: 10.1080/15592324.2014.1000103.
Meinke D, Muralla R, Sweeney C, Dickerman A. Identifying essential genes in Arabidopsis thaliana. Trends Plant Sci. 2008;13:483–91.
Rose AB. The effect of intron location on intron-mediated enhancement of gene expression in Arabidopsis. Plant J. 2004;40:744–51.
Rose AB. Intron-mediated regulation of gene expression. Curr Top Microbiol Immunol. 2008;326:277–90.
Ulmasov T, Murfett J, Hagen G, Guilfoyle TJ. Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. Plant Cell. 1997;9:1963–71.
Žádníková P, Petrášek J, Marhavý P, Raz V, Vandenbussche F, Ding Z, et al. Role of PIN-mediated auxin efflux in apical hook development of Arabidopsis thaliana. Development. 2010;137:607–17.
Abbas M, Alabadi D, Blazquez MA. Differential growth at the apical hook: all roads lead to auxin. Front Plant Sci. 2013;doi:10.3389/fpls.2013.00441.
Lincoln C, Britton JH, Estelle M. Growth and development of the axr1 mutants of Arabidopsis. Plant Cell. 1992;2:1071–80.
Bilsborough GD, Runions A, Barkoulas M, Jenkins HW, Hasson A, Galinha C, et al. Model for the regulation of Arabidopsis thaliana leaf margin development. Proc Natl Acad Sci U S A. 2011;108:3424–9.
Gazzarrini S, McCourt P. Cross-talk in plant hormone signalling: what Arabidopsis mutants are telling us. Ann Bot. 2003;91:605–12.
Cheng Y, Dai X, Zhao Y. Auxin synthesized by the YUCCA flavin monooxygenases is essential for embryogenesis and leaf formation in Arabidopsis. Plant Cell. 2007;19:2430–9.
Overvoorde P, Fukaki H, Beeckman T. Auxin control of root development. Cold Spring Harb Perspect Biol. 2010;doi: 10.1101/cshperspect.a001537.
Aloni R, Schwalm K, Langhans M, Ullrich CI. Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis. Planta. 2003;216:841–53.
Aloni A, Aloni R, Langhans M, Ullrich CL. Role of auxin in regulating Arabidopsis flower development. Planta. 2006;223:315–28.
Keller CP, Stahlberg R, Barkawi LS, Cohen JD. Long-term inhibition by auxin of leaf blade expansion in bean and Arabidopsis. Plant Physiol. 2004;134:1217–26.
Cheng Y, Dai X, Zhao Y. Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis. Genes Dev. 2006;20:1790–9.
Richter R, Behringer C, Zourelidou M, Schwechheimer C. Convergence of auxin and gibberellin signaling on the regulation of the GATA transcription factors GNC and GNL in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2013;110:13192–7.
Noh B, Murphy AS, Spalding E. Multidrug resistance-like genes of Arabidopsis required for auxin transport and auxin-mediated development. Plant Cell. 2001;13:2441–54.
Benková E, Michniewicz M, Sauer M, Teichmann T, Seifertová D, Jürgens G, et al. Local, efflux-dependent auxin gradients as a common module for plant organ formation. Cell. 2003;115:591–602.
Zhao Y. Auxin biosynthesis. Arabidopsis book 2014. doi: 10.1199/tab.0173.
Al-Hammadi AS, Sreelakshmi Y, Negi S, Siddiqi I, Sharma R. The polycotyledon mutant of tomato shows enhanced polar auxin transport. Plant Physiol. 2003;133:113–25.
Chandler JW. Cotyledon organogenesis. J Exp Bot. 2008;59:2917–31.
Ito H, Gray WM. A gain-of-function mutation in the Arabidopsis pleiotropic drug resistance transporter PDR9 confers resistance to auxinic herbicides. Plant Physiol. 2006;142:63–74.
Yu H, Moss B, Jang SS, Prigge MJ, Klavins E, Nemhauser J, et al. Mutations in the TIR1 auxin receptor that increase affinity for Aux/IAA proteins result in auxin hypersensitivity. Plant Physiol. 2013;162:295–303.
Lloyd JP, Seddon E, Moghe GD, Simenc MC, Shiu SH. Characteristics of plant essential genes allow for within- and between-species prediction of lethal mutant phenotypes. Plant Cell. 2015;27:2133–45.
Lee L, Ambaru B, Thakkar P, Marcotte EM, Rhee SY. Rational association of genes with traits using a genome-scale gene network for Arabidopsis thaliana. Nat Biotechnol. 2010;28:149–56.
Zhao D, Ni W, Feng B, Han T, Petrasek MG, Ma H. Members of the Arabidopsis-SKP1-like gene family exhibit a variety of expression patterns and may play diverse roles in Arabidopsis. Plant Physiol. 2003;133:203–17.
Wang R, Estelle M. Diversity and specificity: auxin perception and signaling through the TIR1/AFB pathway. Curr Opin Plant Biol. 2014;21:51–8.
Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Huaming Chen H, Shinn P, et al. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science. 2003;301:653–57.
Clough SJ, Bent AF. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1998;16:735–43.
Lagarde D, Basset M, Lepetit M, Conejero G, Gaymard F, Astruc S, et al. Tissue-specific expression of Arabidopsis AKT1 gene is consistent with a role in K+ nutrition. Plant J. 1996;9:195–203.
Cuéllar T, Pascaud F, Verdeil JL, Torregrossa L, Adam-Blondon AF, Thibaud JB, et al. A grapevine Shaker inward K+ channel activated by the calcineurin B-like calcium sensor 1-protein kinase CIPK23 network is expressed in grape berries under drought stress conditions. Plant J. 2010;61:58–69.
Bartel PL, Fields S. Analyzing protein-protein interactions using two-hybrid system. Methods Enzymol. 1995;254:241–63.
Gietz RD, Schiestl RH. Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier. Yeast. 1991;7:253–63.
Daram P, Urbach S, Gaymard F, Sentenac H, Chérel I. Tetramerization of the AKT1 plant potassium channel involves its C-terminal cytoplasmic domain. EMBO J. 1997;16:3455–63.
We wish to thank Rémy Gibrat for pictures of plants on vertical plates, Nadine Paris for Fig. 3c, Geoffrey Duby for Fig. 1k, Hugues Baudot, Thierry Dessup and José Garcia for taking care of greenhouse plants. We also thank the Salk Institute Genomic Analysis Laboratory for providing the sequence-indexed Arabidopsis T-DNA insertion mutants, and the Nottingham Arabidopsis Stock Center (NASC) for their distribution. This work was partly funded by the GABI-Genoplante project “Functional genomics of membrane transporters” (AF2001065).
No competing interest.
ATF and MB conducted molecular biology experiments, DTL obtained the confocal microscopy images of roots, IC was involved in molecular biology experiments, creation of plant lines and their analyses, and wrote the paper. IG performed the Q-PCR experiments. ATF, DTL, HS and IG contributed to analyses of results and critical reading of the manuscript. All authors have read and approved the final version of the manuscript.
Martin Boeglin and Anja Thoe Fuglsang contributed equally to this work.
About this article
Cite this article
Boeglin, M., Fuglsang, A.T., Luu, DT. et al. Reduced expression of AtNUP62 nucleoporin gene affects auxin response in Arabidopsis . BMC Plant Biol 16, 2 (2016). https://doi.org/10.1186/s12870-015-0695-y
- Nuclear pore complex