Fig. 5
EGTA-inhibition of ATP transport via AtAPC2 and reactivation by MgCl2 and CaCl2. Transport of 50 μM [α32P]-ATP into Pi loaded vesicles in absence of EGTA, MgCl2 and CaCl2 was set to 100 % (control; red line). Transport in presence of 200 μM EGTA, 200 μM EDTA and the given concentrations (in mM) of MgCl2 or CaCl2 was calculated accordingly. Generally transport was allowed for 10 min. However, EGTA-inhibited transport was reactivated after 10 min of uptake by subsequent addition of MgCl2 or CaCl2 and transport was again allowed for 10 min (EGTA pretreatment and reactivation; light gray bars). Data represent net values (ATP import in exchange with Pi minus background values of non-loaded proteoliposomes) and are the mean of three independent experiments. Standard errors are indicated