Fig. 3

Phylogenetic analysis of CYP719 gene candidates from twenty BIA-accumulating plant species. Red text denotes characterized genes or enzymes used as tBLASTn queries for transcriptome mining. Black text denotes uncharacterized gene candidates identified through mining (>40 % identity to queries). Bootstrap values for each clade were based on 1000 iterations. Each candidate is labeled with respective species abbreviation (e.g. AME, Argemone mexicana; see Table 1) and candidate number (e.g. CYP719-1). Each query is labeled according to species (additional species: CJA, Coptis japonica; PSO, Papaver somniferum) with CYP719 subfamily and gene number indicated (e.g. CYP719B1, salutaridine synthase; see Fig. 1). Outgroup is CYP17A1 from Homo sapiens (HSA). Amino acid sequences for candidates, queries, and outgroups are found in Additional file 6