Fig. 1

Ethylene induction of ARGOS family members. a Ethylene dose-dependency for induction varies among family members. Green seedlings were treated for 2 h in the presence or absence of the indicated ethylene concentrations, RNA isolated, and gene expression determined by qRT-PCR based on three biological replicates. Expression is shown relative to the maximal induction observed for each gene. Significant dose-dependent differences in expression for each gene are based on an analysis of variance applying Bonferroni correction post-test comparisons (p < 0.05); designations with the same letter exhibit no significant difference. Error bars indicate SE; error bars not shown if smaller than symbol. b Effect of ethylene-insensitive mutations on gene induction. Induction was examined in wild-type (wt) and the ethylene-insensitive mutants etr1-1, ein2-1, and ein3;eil1 using 2-h treatments of green seedlings in the presence or absence of 10 μL L⁻¹ ethylene. Gene expression was determined by semi-quantitative RT-PCR. c Time course for ethylene induction of ARGOS and ARL, as compared to the receptors ETR2, ERS1, and ERS2. Green seedlings were treated for indicated times with 10 μL L⁻¹ ethylene and gene expression determined by semi-quantitative RT-PCR. d Auxin induction of ARGOS family members is ethylene-dependent. Wild-type or etr1-1 green seedlings were treated for 3 h in the absence or presence of 50 μM ACC or 5 μM NAA. Gene expression was determined by semi-quantitative RT-PCR