- Research article
- Open Access
Origin and evolution of Petrocosmea (Gesneriaceae) inferred from both DNA sequence and novel findings in morphology with a test of morphology-based hypotheses
© Qiu et al. 2015
- Received: 27 November 2014
- Accepted: 4 June 2015
- Published: 3 July 2015
Petrocosmea Oliver (Gesneriaceae) currently comprises 38 species with four non-nominate varieties, nearly all of which have been described solely from herbarium specimens. However, the dried specimens have obscured the full range of extremely diverse morphological variation that exists in the genus and has resulted in a poor subgeneric classification system that does not reflect the evolutionary history of this group. It is important to develop innovative methods to find new morphological traits and reexamine and reevaluate the traditionally used morphological data based on new hypothesis. In addition, Petrocosmea is a mid-sized genus but exhibits extreme diverse floral variants. This makes the genus of particular interest in addressing the question whether there are any key factors that is specifically associated with their evolution and diversification.
Here we present the first phylogenetic analyses of the genus based on dense taxonomic sampling and multiple genes combined with a comprehensive morphological investigation. Maximum-parsimony, maximum likelihood and Bayesian analyses of molecular data from two nuclear DNA and six cpDNA regions support the monophyly of Petrocosmea and recover five major clades within the genus, which is strongly corroborated by the reconstruction of ancestral states for twelve new morphological characters directly observed from living material. Ancestral area reconstruction shows that its most common ancestor was likely located east and southeast of the Himalaya-Tibetan plateau. The origin of Petrocosmea from a potentially Raphiocarpus-like ancestor might have involved a series of morphological modifications from caulescent to acaulescent habit as well as from a tetrandrous flower with a long corolla-tube to a diandrous flower with a short corolla-tube, also evident in the vestigial caulescent habit and transitional floral form in clade A that is sister to the remainder of the genus. Among the five clades in Petrocosmea, the patterns of floral morphological differentiation are consistent with discontinuous lineage-associated morphotypes as a repeated adaptive response to alternative environments.
Our results suggest that the lineage-specific morphological differentiations reflected in the upper lip, a functional organ for insect pollination, are likely adaptive responses to pollinator shifts. We further recognize that the floral morphological diversification in Petrocosmea involves several evolutionary phenomena, i.e. evolutionary successive specialization, reversals, parallel evolution, and convergent evolution, which are probably associated with adaptation to pollination against the background of heterogeneous abiotic and biotic environments in the eastern wing regions of Himalaya-Tibetan plateau.
- DNA sequence
- Floral morphology
- Himalaya-Tibetan plateau
In current plant systematics, research activity tends to begin with phylogenetic reconstruction based on DNA sequence data. Molecular systematics has revolutionized traditional plant systematics and classification. However, the morphological support for such changes has often been absent, or consisted of ad hoc explanations. In many cases, the few morphological characters used to support molecular phylogenies are selected from the characters that were used to initially describe the taxa, rather than novel characters from active morphological and anatomical research. This situation is mainly due to the misunderstanding that everything in morphology has been completed . On the contrary, numerous morphological characters are yet unexplored, especially in tropical groups. Many of these characters may reflect the evolutionary histories of these taxa and serve as a complement to molecular phylogenies.
Petrocosmea Oliv. (Gesneriaceae, Didymocarpoideae sensu Weber et al. 2013)  contains 38 species with four non-nominate varieties, all mostly distributed in southwestern China with several species in Northern Myanmar and Thailand, and Northeastern India [3–6]. The genus has been divided into three subgeneric sections. Hemsley (1899)  erected section Anisochilus Hemsl. because two species, P. iodioides Hemsl. and P. minor Hemsl., have an upper lip that is much shorter than the lower lip making them distinctive from P. sinensis Oliv.. Craib (1919)  made the first revision of the genus with 15 species placing them in sections Petrocosmea Craib and Anisochilus. In the second revision that included 27 species and four varieties, Wang (1985)  principally followed Craib (1919)  but established sect. Deinanthera W. T. Wang. Members of this latter section have anthers constricted near the apex that create a short thick beak. Wang’s classification system has been followed by later authors [3–5].
Few morphological characters were utilized in the sectional divisions and species descriptions, probably because most information was lost on dried specimens. For example, the subgeneric rankings were roughly based on the length ratios of the upper lip (two upper corolla lobes) to the lower lip (two lateral and one lower corolla lobes), and the degree of fusion of the two upper corolla lobes [3–5, 8, 9]. From the description of different sections and species, it would appear that the flowers are morphologically simple in Petrocosmea.
Since Petrocosmea was describecd , no molecular systematic study has focused on the phylogeny of Petrocosmea except for a few species that have been sampled in molecular phylogenetics at higher ranks in Gesneriaceae [12–15]. A phylogenetic reconstruction based on DNA sequence data from multiple loci would enhance our understanding of morphological diversity in relation to evolutionary history and test the interpretation of morphological evolution and homology in this genus. In addition, the presently distributed area of Petrocosmea in the northern Myanmar and Thailand, northeastern India and southwestern China is just located in the eastern wing region of the Himalaya-Tibetan plateau. This is where the Hengduan Mountains, that consist of rugged terrain with high mountains alternating with several deep gorges, runs parallel north to south. The Hengduan Mountains have not only been widely considered an important center of survival, but also a well-known region of speciation and evolution in the world [16, 17]. It would be interesting to know whether the origin and diversification of Petrocosmea are related to this heterogeneous ecogeographical environment.
In the present study, we analyzed a multi-gene dataset including two nuclear (ITS, Petrocosmea CYCLOIDEA1D (PeCYC1D)) and six plastid regions (atpI-H, matK, trnH-psbA, rps16, trnL-trnF, trnT-trnL) from 35 species and three non-nominate varieties of Petrocosmea with two species of Raphiocarpus plus an additional species of Boea and two species of Streptocarpus as outgroups. Since we had continuously carried out field observation and had collected living plants of most Petrocosmea species in the greenhouse, we also conducted a comprehensive investigation on the flower morphology of Petrocosmea by dissecting the flower into a series of units for detailed comparison and analyses. Forty-one morphological characters from both vegetative and floral organs were analyzed to reconstruct the relationship within Petrocosmea alone and combined with molecular data. Our objectives in this study are (1) to test the monophyly of the genus; (2) to explore the morphological origin and differentiation pattern of Petrocosmea; (3) to interpret the evolutionary significance of the morphological differentiation within a robust phylogenetic context linked to both biotic and abiotic environment; and finally (4) to evaluate the newly observed vs. traditionally utilized morphological characters in relation to the role of morphological data in phylogenetic reconstruction.
Analyses of DNA sequence and morphological data separately
The combined cpDNA matrix, which comprises six chloroplast regions of trnL-F, matK, rps16, atpI-atpH, trnH-psbA, and trnT-L, had aligned sequences of 5662 bp, of which 4719 (83.35 %) were constant, 560 (9.89 %) were variable but uninformative, and 383 (6.76 %) were parsimony informative. We were unable to amplify cpDNA regions from P. confluens. Modeltest indicated GTR + G as the best-fit model for the cpDNA sequence data. The strict consensus of 6 trees yielded by MP (Maximum Parsimony) analysis (L = 1182, CI = 0.884, RI = 0.873) was generally congruent with the ML (Maximum Likelihood) tree and the majority rule BI (Bayesian Inference) tree in the topology (Additional file 1: Figure S2). Support values less than 50 % are marked with asterisk.
In the nuclear DNA analysis with P. confluens added to the matrix, the ILD (incongruence length different) test gave a p value of 0.42, indicating that the sequence data from ITS and PeCYC1D were congruent. The combined nuclear DNA matrix of ITS and PeCYC1D consisted of 1662 bp, of which 1213 (72.98 %) were constant, 228 (13.72 %) were variable but uninformative, and 221 (13.3 %) were parsimony informative. Modeltest indicated GTR + G as the best-fit model for the combined nuclear DNA data. The strict consensus of eight trees from MP analysis (L = 642, CI = 0.872, RI = 0.849) was congruent with the ML tree and the majority rule consensus BI tree (Additional file 1: Figure S3).
The MP-ML-BI tree of the combined cpDNA and nuclear DNA datasets was similar to the cpDNA and nuclear DNA trees but with stronger support (Figs. 2, Additional file 1: Figure S2-S3). The combined cpDNA and nuclear DNA tree comprises five main clades labeled A–E (Fig. 2). Each clade receives strong or maximum support, and they are grouped together successively by strong to maximum support (Fig. 2).
For the analysis of the morphological data, Forty-one morphological characters were coded. The strict consensus of 125 trees yielded from the MP analysis (L = 82, CI = 0.842, RI = 0.972) was congruent with the majority rule consensus BI tree (Additional file 1: Figure S4). Similar to the DNA trees, the morphological tree comprises five major clades including the same species as the molecular based trees. However, most nodes within the major five clades have weak to moderate support with frequent polytomies.
Analysis of combined DNA sequence and morphological data
Clade B contains eight taxa (P. coerulea, P. begoniifolia, P. melanophthalma, P. confluens, P. hexiensis, P. duclouxii, P. sichuanensis, and P. mairei var. intraglabra) of sect. Anisochilus sensu Wang (1985) , and is a well-supported clade sister to clades C-D with maximum support. Petrocosmea mairei var. intraglabra and P. sichuanensis as a pair of sister species with maximum support are strongly supported to come together successively with P. duclouxii (MP-BS (bootstrap) =96 %; PP (posterior probabilities) =100 %), P. hexiensis (MP-BS = 99 %; PP =100 %), and P. confluens (MP-BS = 98 %; PP = 100 %). Petrocosmea coerulea and P. melanophthalma as sister species with moderate support (MP-BS = 78 %; PP = 98 %) are further clustered together with P. begoniifolia with MP-BS = 70 % and PP = 100 %). The two branches in clade B are further joined together with strong support (MP-BS = 97 %; PP = 100 %). The species of clade B are defined by their short upper lips with semiorbicular corolla lobes. The morphological synapomorphies of clade B also include two upper corolla lobes highly reflexed backward with two purple spots on the lower side of the corolla-tube below the filaments (Fig. 1). Apparently, P. mairei var. intraglabra is a species apart from P. mairei var. mairei which is nested in clade D (Figs. 2-3).
Clade C includes eight taxa (P. iodioides, P. martinii var. leiandra, P. martinii var. martinii, P. minor, P. sericea, P. shilinensis, P. xingyiensis and P. huanjiangensis) of sect. Anisochilus and two species (P. grandiflora and P. yanshanensis) of sect. Petrocosmea. There are two lineages in Clade C with maximum support. In one lineage, P. grandiflora and P. yanshanensis as strongly supported sister species (MP-BS = 97 %; PP = 100 %) are grouped in sequence with P. sericea (MP-BS = 98 %; PP = 100 %), P. martinii var. martini (MP-BS = 99 %; PP = 100 %), and maximally supported sister species of P. iodioides and P. martinii var. leiandra. In another lineage, P. minor and P. shilinensis are sister to each other (MP-BS = 71 %; PP = 97 %), and further grouped with P. xingyiensis by moderate support (MP-BS = 73 %; PP = 100 %), and together they are sister to P. huanjiangensis with strong support (MP-BS = 98 %; PP = 100 %).
Clade D comprises six taxa (P. forrestii, P. mairei var. mairei, P. barbata, P. cavaleriei, P. xanthomaculata, and P. longipedicellata) of sect. Anisochilus and two newly described species P. nanchuanensis and P. glabristoma with strong support (MP-BS = 98 %; PP = 100 %). Petrocosmea nanchuanensis is sister to a maximally supported branch containing P. barbata, and P. longipedicellata gathered together by strong support (MP-BS = 91 %; PP = 100 %) with two maximally supported sister species, P. cavaleriei and P. xanthomaculata. These five species as a maximum supported branch are further united with three well resolved sister species P. glabristoma, P. forrestii and P. mairei var. mairei. The species in clade D have a generally similar bilateral corolla to the species in clade B. However, the two lobes in the upper lip are extended forward rather than reflexed backward. In addition, they can also be easily recognized by two bright yellow spots or cicatrices on the lower lip and hairs on the upper lip in the corolla throat (Fig. 1).
Five species (P. nervosa, P. oblata, P. flaccida, P. sinensis, and P. qinlingensis) of sect. Petrocosmea form clade E with maximum support. In clade E, P. oblata and P. flaccida are sister with maximum support and these two are grouped with another set of sister species, P. sinensis and P. qinlingensis, with strong support (MP-BS = 90 %; PP = 100 %). Petrocosmea nervosa is sister to the remaining species in Clade E with maximum support. The species of clade E all share a large bilobed upper lip that is equal or almost equal to the trilobed lower lip (Fig. 1). Correspondingly, their styles are generally located in the center of the flower. In addition, the longitudinal anthers, and three yellow spots on the upper side of the corolla tube below the filaments are unique to the species of clades D and E, supporting their sister relationship.
Ancestral area and character state reconstructions
A series of novel morphological traits are correlated with cladogenetic events in Petrocosmea. These morphological novelties are mainly reflected in the size and shape of the upper lip. In clade A, the two upper corolla lobes are slightly smaller than the three corolla lobes of the lower lip, generating a moderate floral zygomorphy as in Raphiocarpus. In clade B, the two upper corolla lobes are remarkably reduced relative to the three lobes of the lower lip. In clade C, the two much shortened upper corolla lobes are fused and extremely specialized. In clade D, even though the upper lobes are in general similar to those in clade B in size, they are extended forward with a flat face, contrasting with the two upper corolla lobes reflexed backward in clade B. The flowers in clade E are nearly actinomorphic, reflected in the equal length of the upper and lower lips, a deep sinus among the five corolla lobes and a much shortened corolla tube (Fig. 1). These morphological variants in the size and shape of the upper lip are consistent with a series of counterparts in other floral organs, such as character of corolla throat, length ratio of corolla tube to corolla lobes, inflation of the lower part of the corolla tube, position of the anther and filament relative to the ovary and style and type of anther dehiscence, exsertion of the style with curvature type of style tip, and dorsoventrally equal/unequal development of the ovary (Figs. 1, 4, 8).
The monophyly of Petrocosmea is well supported by both molecular and morphological data so far as our current sampling is concerned (Figs. 2-3, Additional file 1: Figure S1-S3). The flowers of Petrocosmea are characterized by a short corolla tube with a length of only 3-6 mm. This short tube is remarkably different from the flowers of many other Gesneriaceae where corolla tubes often are over 2 cm long , but is similar to species of Saintpaulia . Petrocosmea, with a combination of synapomorphies (perennial stemless herbs, bilateral-diandrous flowers with a short corolla tube and two fertile stamens), is clearly distinguished from its sister group Raphiocarpus (subshrub, bilateral-tetrandrous flowers with a long corolla tube over 4 cm long, and four fertile stamens). However, the molecular phylogeny herein does not support the traditional classification of Petrocosmea as divided into three sections (Petrocosmea, Anisochilus and Deinanthera). The species of the three sections are scattered across different branches in the phylogenetic trees from all analyses; none of the three sections are recovered as monophyletic regardless of the source of data used for the phylogenetic analyses. In contrast, our molecular data show that Petrocosmea consists of five clades corroborated by morphological data as prementioned.
Origin of Petrocosmea
The present molecular phylogeny represents the first major step toward understanding evolution in Petrocosmea. Further analyses of morphological characters in light of the molecular phylogeny will enhance our understanding of the morphological origin and diversification in relation to the evolutionary history of this genus. A growing amount of evidence from phylogenetic studies shows that the acaulescent and diandrous-flowered Petrocosmea might have proceeded from a caulescent and tetrandrous-flowered Raphiocarpus-like ancestor [14, 15]. The tetrandrous flowers with only the mid-upper stamen aborted have been considered an ancestral state in Didymocarpoideae [6, 14, 15]. The morphological evolutionary shift from tetrandrous to diandrous flowers has occurred several times in the Old World Gesneriaceae, such as the shift from tetrandrous Oreocharis to Opithandra with only two lateral fertile stamens and from tetrandrous Anna to diandrous Lysionotus with both the mid-upper and lateral stamens aborted [14, 15, 18]. This morphological shift in stamen number usually involves only the increase of sterile stamens from the mid-upper to both the mid-upper and lateral/ventral stamens with the length of corolla tube unchanged in most groups of Gesneriaceae. Therefore, most genera are characterized by a long corolla tube both in tetrandrous and diandrous flowers in Gesneriaceae [5, 6]. However, the flowers of Petrocosmea are not only diandrous but also have a short corolla tube of only 3-6 mm as well as acaulescence. The plants of clade A retain a caulescent habit with ascendant leaves. The plants of other clades of Petrocosmea, in contrast, are characterized by short rhizomes with leaves spreading on the ground (Fig. 7a). The caulescent habit is correlative with their moderately zygomorphic flowers with a relatively long corolla-tube found in species of clade A that is distinctively different from the strongly zygomorphic flowers and short corolla tube in clades B-D.
Ancestral area reconstruction indicates the origin of Petrocosmea in the boundary area of India, Myanmar, Thailand and China, lying east and southeast of Himalaya-Tibetan plateau. This eastern wing region of the Himalaya-Tibetan plateau is one of the most geologically active areas in the world, covered with pure carbonate substrate [19–21]. These limestone areas are characteristic of fluctuating ecological environments with an alternation between severe erosion in the rainy season and extreme drought in the long dry season that are stressful for plant growth [19, 21]. The acaulescent and subsequent rosette habit of Petrocosmea might have evolved in response to selective pressures imposed by extreme fluctuation of seasonal climate and ecological conditions. Habitat usually exerts strong influence on vegetative growth adapted for plant survival . However, little is known about the driving force behind the evolution from a long to short corolla tube in the origin of Petrocosmea. The shift in corolla tube length is probably related to changes in the insect fauna.
Functional and evolutionary implications of the lineage-specific morphological differentiation
As outlined above, a series of novel morphological traits are correlated with the cladogenetic events in Petrocosmea, all are first documented in present study. They are mainly reflected in the upper lip, i.e. the two upper corolla lobes, in size and shape. Floral zygomorphy (bilateral symmetry) is one key innovation associated with the adaptive radiation of angiosperms because it promotes the coevolution between plants and animals [23–26]. The evolution of floral zygomorphy has been widely considered a major trend in the phylogeny of angiosperms, in which zygomorphy has played a key role in generating the diversity inherent to many large and successful angiosperm clades [23, 26, 27]. Zygomorphy is also found to be one of the three main factors associated with the geographical distribution of diversification hotspots in angiosperms (two other factors are noncontiguous distribution and altitude) [28, 29]. For example, frequent pollinator shifts are correlated with rapid lineage diversification in the flora of southern Africa that has exceptional species richness and endemism . Floral zygomorphy usually promotes reproductive isolation by discrimination in favor of specific pollinators, such as two sympatric species in Mimulus marked by different zygomorphic flowers that are specifically pollinated by bees or hummingbirds . In Malpighiaaeae, the floral arrangement rotates 36° between zygomorphic flowers as the pollinator shifts from the oil-bee to xylocopine-bee .
In insect-pollinated zygomorphic flowers, the lower lip often functions as a platform for the landing of visiting insects. However, the upper lip plays a key role in attracting or permitting specific pollinators to visit by specialized petal size and shape. Therefore, the lower lip is generally consistent in morphology while the upper lip exhibits variation and specialization in both shape and size [24, 33]. It is especially true for Petrocosmea, in which the upper lip tends to be shortened and specialized from clade A through clade B to clade C in size and shape, especially the carinate-plicate shape in clade C, with coordinated variation of correlative characters. Ancestral state and area reconstructions demonstrate that the floral morphological specialization is accompanied by the geographical dispersal from the boundary area of India, Myanmar, Thailand and China, lying east and southeast of Himalaya-Tibetan plateau, to southwestern China, especially the Hengduan Mountain-Yungui plateau areas where Petrocosmea is highly diversified. The floral transition from moderate to extremely strong zygomorphy may reflect a pollinator or pollinator-behavior shift likely towards more specialized pollination, which is the major evolutionary trend of the floral zygomorphy in many clades of angiosperms [23, 26, 27]. However, the upper lip demonstrates a reversal from the extremely zygomorphic flowers in clade C through clade D to the almost actinomorphic flowers in clade E, as well as a parallel evolutionary pathway within clade C, correlated with a series of other floral morphological differentiations. This evolutionary reversal also frequently occurs in other groups of Gesneriaceae accompanied by pollinator shifts towards generalized pollination . For example, the tubular zygomorphic flowers specifically pollinated by hummingbirds proceeds to subcampanulate flowers with generalized pollination in Gesnerieae , as well as the flat-faced actinomorphic flowers of Ramonda evolved from the tubular zygomorphic flowers of Haberlea that switched to generalist pollinators [15, 35, 36]. The floral morphological transition with pollinator shift from specialist to generalist is usually related to the evolution of the reproductive assurance mechanism when specialist pollinators are absent or rare . It may apply to the floral evolutionary reversal from clade C to clade E and within clade C in Petrocosmea. The lineage-specific differentiation reflected in the upper lip with correlative characters might be related to the evolution of functional morphology to optimize pollination process in the genus Petrocosmea. The elaborate morphology of the upper lip characteristic of carinate-plicate shape in clade C may represent a functional innovation for the occurrence of wholly novel or more effective specialist pollinators.
Given that the phenotypic variation in Petrocosmea mainly involves floral rather than vegetative traits, the diversification of Petrocosmea is likely a product of concerted evolution associated with adaptation to different groups of pollinators rather than a direct response to physical environmental variables. Petrocosmea is a mid-sized genus but exhibits extreme diverse floral variants mainly reflected by various forms of the upper lip apparently shaped by different pollinators as shown in other groups of Gesneriaceae. In addition, the floral morphological diversification in Petrocosmea involves several evolutionary phenomena, i.e. evolutionary successive specialization from clade A to clade C, reversal from clade C to clade E as well as within clade C, parallel evolution reflected by the parallel branches switching to moderate zygomorphy or almost actinomorphy in clade C, and convergent evolution demonstrated by floral similarity between some branches in clades C and E. These evolutionary phenomena are probably all associated with adaptation to pollination under the background of heterogeneous abiotic and biotic environments in the eastern wing regions of Himalaya-Tibetan plateau. Petrocosmea may represent an ideal model for the research of floral evolution related to plant-insect coevolution. Therefore, it merits further study in pollination biology to find whether specific pollinators or pollinator behaviors are responsible for the lineage-specific morphological differentiation, especially for the upper lips, in Petrocosmea.
Utility of morphological characters
According to Craib (1919)  and Wang (1985, 1990, 1998) [3, 4, 9], the relative length of the upper and lower lips was the dominant principle to divide sections within Petrocosmea. Therefore, all species in clade E and some species in clade C with equal or nearly equal upper and lower lips were grouped in sect. Petrocosmea, which are traditionally considered the most primitive in the genus . Most species of clades A, B, C, D with the upper lip shorter than the lower lip were placed in sect. Anisochilus, and later, the species of clade A were further identified as the most advanced group due to their unique anther morphology and moved to sect. Deinanthera .
Some authors hold the opinion that morphological data are problematic in reconstructing phylogenetic trees because morphology is frequently convergent and therefore often misleading [37, 38]. Nevertheless, others argue that morphological data synergistically contribute to phylogenetic trees because of their low intrinsic homoplasy and the problems in resolving homology in morphology can be solved through methodological development and examination using modern tools [39–41]. Abundant morphological characters have been utilized in traditional systematics for more than 200 years, thus the term “morphological feature” is a concept that a lot of people infer as characters that were traditionally used . In this sense it could be difficult to avoid convergence and confusion about different evolutionary status when wholly indiscriminately using them. Relying only on characters that have been used in the traditional classification systems within Petrocosmea reveals homoplasy in these traits based on the phylogenetic analyses in this study. However, the same five clades that were recovered based on DNA sequence data were also recovered using a purely morphological data set that utilized numerous character states that had not previously been considered in the classification within Petrocosmea, albeit the fewer number of morphological characters did not yield the same level of support for the clades. Our results highlight that morphological features are still important and relevant in resolving phylogenetic relationships, but must be evaluated in concert and not be used in isolation for the "value" of any one character. We here distinguish clade A from other clades based on the retention of a caulescent habit and moderate floral zygomorphy. In contrast to placing them in the same section only based on their similar length of the upper lip in traditional classification, we distinguish the plants of clade B, C (the main branch) and D from each other with character combination of upper to lower lip ration of 1:2 with the upper lip reflexed backward in clade B, upper to lower lip of 1:4 with the upper lip folded laterally to specially form a carinate-plicate shape in the main branch of clade C and upper to lower lip ratio of 1:2 with the upper lip extended forward in clade D. We further interpret the equal length of the upper and lower lip in clade E as well as some branches in clade C as a floral reversal rather than an ancestral feature in the traditional classification according to the molecular phylogeny herein. These recognitions of the major distinctive morphological characters are based on the combination of traditionally used characters and our novel findings in morphology, and reevaluation on the ground of new hypothesis, correlated with a series of other morphological traits. Any one morphological character is not problematic in and of itself but can circumscribe non-monophyletic groups when used in isolation, a priori, as being more useful than other characters.
Traditional investigations of morphology should be renewed or extended in the form of new hypotheses for phylogenetic reconstruction and evolutionary origin. According to Bybee et al. (2010) , morphological characters were not only infrequently (only 10.5 %) used in recent phylogenetic reconstructions, but the selection of characters were largely unoriginal and untested for their synapomorphies. It is likely that some so-called morphological synapomorphies in traditional systematics are in fact morphological similarities as a result of convergent evolution. Given the large amount of morphological characters utilized in traditional systematics, there is an urgent need to teach old dogs new tricks in present phylogenetic reconstructions.
In contrast to Petrocosmea actually exhibiting extremely diverse floral variation, few morphological characters have been described in the traditional system with poor subgeneric classification. We conduct the first phylogenetic analyses in Petrocosmea based on dense taxonomic sampling and multiple loci from two nuclear and six chloroplast DNA regions, which support the monophyly of Petrocosmea and recover five major clades within the genus. We further carry out a comprehensive investigation on the flower morphology with living plant material in Petrocosmea and find a series of novel morphological traits that are specific to the five respective clades. Reconstructions of ancestral states of twelve morphological characters strongly support five clades revealed by the molecular phylogeny, suggesting these newly observed morphological traits have phylogenetic significance. Phylogenetic analyses and ancestral state reconstructions suggest that the acaulescent Petrocosmea with diandrous flowers and short corolla tubes might have proceeded from the caulescent Raphiocarpus-like ancestor with tetrandrous flowers and long corolla tubes. Ancestral area reconstruction shows that the geographic origin of Petrocosmea lies east and southeast of the Himalaya-Tibetan plateau. Functional and evolutionary analyses of floral morphology indicate that the lineage-specific floral differentiation reflected in the upper lip in Petrocosmea are likely adaptive responses to the shift of pollinators or pollinator behaviors, especially the highly specialized structure of the upper lip, a carinate-plicate shape in clade C first recognized herein. We find that the floral morphological diversification in Petrocosmea involves several evolutionary phenomena, i.e. evolutionary successive specialization, reversal, parallel evolution, and convergent evolution, which are probably associated with plant-insect coevolution in the heterogeneous abiotic and biotic environments in the eastern wing regions of Himalaya-Tibetan plateau. Further detailed research in pollination biology with ecogeography-associated analyses would shed light on mechanisms underlying the floral evolution and diversity of Petrocosmea as an adaptive coevolution responding to local environmental changes. Our results also highlight the importance that morphological features, when evaluated in concert, and through active research to discover new characters, would enhance our understanding of the relationships revealed by molecular phylogeny.
Thirty-five species and three non-nominate varieties including all three sections of Petrocosmea sensu Wang (1985)  were sampled. Attempts were made to find suitable material of P. condorensis Pellegr., P. kingii (Clarke) Chatterjee and P. formosa B. L. Burtt as well as P. oblata var. latisepala W. T. Wang, but without success. All materials for DNA extraction came from silica-dried or fresh leaves except for Petrocosmea confluens W. T. Wang and P. grandiflora Hemsl. for which herbarium specimens at PE and KEW, respectively were used. The voucher information of all sampled taxa and GenBank accession numbers are listed in Additional file 1: Tables S1-S2.
Outgroup choice for phylogenetic study
To determine the most appropriate outgroup for the phylogenetic study of Petrocosmea, a large number of related species from 23 genera of Gesneriaceae were sampled. Twenty genera from other Didymocarpeae, one from Trichosporeae which may be a close relative of Didymocarpeae [12, 13, 43], one from Cyrtandreae, and one from Epithemateae were included. Antirrhinum majus (Plantaginaceae) and Tetranema mexicanum (Scrophulariaceae) were used as outgroups in preliminary analyses based on trnL-F and ITS (Additional file 1: Table S2). The result showed that Petrocosmea is well supported as monophyletic and sister to two species of Raphiocarpus from Didymocarpeae with strong to moderate support (Additional file 1: Figure S1). Therefore, Raphiocarpus begoniifolius and Raphiocarpus petelotii were chosen as outgroups with the additional inclusion of one species of Boea and two species of Streptocarpus for the subsequent phylogenetic analyses in all data sets for this study.
DNA extraction, PCR amplification, and sequencing for phylogenetic study
Total genomic DNA was extracted from silica-gel-dried, fresh or herbarium specimen leaf materials using the CTAB method of Rogers and Bendich (1988)  and used as the templates in the polymerase chain reaction (PCR).
The atpI-atpH, matK, trnH-psbA, rps16 intron, trnL-F, trnT-L, and the entire nuclear ribosomal DNA ITS regions were amplified using atpI/atpH , matK-AF/trnK-2R [46, 47], trnH/psbA [48, 49], rps16-2 F/rps16-R3 , c/f , a/b , ITS-1/ITS-4 , respectively. To amplify the PeCYC1D region, a pair of primers were used: forward PD1 (5’–CCC ACA AGA AAT AAT GCT TAG C–3’) and reverse PD2 (5’ – AGC ACA GAT GCC AAA AGA TTC – 3’). The PCR products were purified using Tian quick Midi Purification Kit (Tiangen Biotech, Beijing, China) following the manufacture’s protocol and were directly sequenced. The sequencing primers are the same as amplification primers except matK and PeCYC1D regions. A reverse primer matK-8R  was added in the matK region sequencing, and a forward primer F1d (5’–TCA TCC TCC TCA GGT TTC ACA G–3’) and the reverse primer R  were used in the PeCYC1D sequencing.
DNA sequence alignment and phylogenetic analyses
Sequences were aligned using Clustal X1.83  and adjusted manually using BioEdit5.0.9 . All combined DNA data were analyzed with maximum parsimony (MP), maximum likelihood (ML) and Bayesian inference (BI) methods, which were implemented in PAUP**4.0b10 , RAxML 8.1.11 , and MRBAYES version 3.0b4 , respectively.
For MP analysis, all characters were given equal weight and character states were unordered. Heuristic searches were performed with 1000 replicates of random addition, one tree held at each step during stepwise addition, tree-bisection-reconnection (TBR) branch swapping, Multrees in effect, and steepest descent off. Bootstrap support  for each clade was estimated from 1000 heuristic search replicates as described above.
For ML analysis, the optimal model and parameters were determined under the Akaike information criterion (AIC) in Modeltest 3.06 . A BIONJ tree was employed as a starting point . Statistical support for the node on the ML tree was estimated by 1000 replicates of bootstrap analyses.
In the BI analysis, the model choice of nucleotide substitution was the same as described in ML analysis. Four chains of the Markov Chain Monte Carlo were run each for 10,000,000 generations and were sampled every 10,000 generations. For each run, the first 200 samples were discarded as burn-in to ensure that the chains reached stationary. In the majority rule consensus from Bayesian analysis, posterior probability (PP) was used to estimate robustness.
For combined sequence data, the incongruence length difference (ILD) test  as implemented in PAUP* 4.0b10  was performed to assess character congruence between cpDNA data and nDNA data, with 1000 replicates, each with 100 random additions with TBR branch swapping. The p value was used to determine whether the two data sets contained significant incongruence (0.05).
Constructing a morphological character matrix and reconstructing the ancestral state of some selected morphological characters
The morphological dataset is based on 41 characters, of these, 25 are floral and important traits previously used for subgeneric classification within Petrocosmea (see Additional file 1: Appendix S1). The morphological data and the combined matrix of DNA plus morphological data were analyzed with MP and BI methods. The Mk1 model was used for the morphological characters in BI. Characters are equally weighted and the states were unordered.
The evolution of twelve diagnostic characters (for detail see Results) was analyzed on the posterior set of trees from the combined molecular MP analysis. The analysis was performed using unordered maximum parsimony as implemented in Mesquite ver. 3.02 (available from http://mesquiteproject.org). The results are summarized on the majority rule consensus tree of the posterior set of trees.
To reconstruct the possible ancestral ranges of Petrocosmea, we conducted an S-DIVA analysis  using the software package RASP . By utilizing the bootstrap distribution of trees resulting from a MP analysis and generating credibility support values for alternative phylogenetic relationships, the S-DIVA method can minimizes the phylogenetic uncertainties [62, 64, 65].
We used the most parsimonious tree generated from analysis of combined cpDNA and nDNA data as a final representative tree. Four geographic regions were coded: Region A, the border region of China, Thailand, India, and Myanmar, lying east and southeast of Himalaya-Tibetan Plateau; Region B, the Hengduan Mountain-Yunnan Plateau region in southwestern China; Region C, The central China; Region D, the north-central China. By loading the representative tree file and the distribution file based on the geographic region codes as mentioned above, the statistical Dispersal-Vicariance Analysis (S-DIVA) was executed in the software package RASP. Ancestral areas were reconstructed with the “max areas” constrained to three because most species occur in fewer than three areas.
The geographical distribution was generated by ARCGIS 10.2(ESRI,US). Locations of Petrocosmea distribution were obtained from collection records and herbarium. The transition from location to longitude and latitude was carried out online (www.gpsspg.com).
Deposition of phylogenetic data in Treebase
All the phylogenetic data used in this study have been deposited to the Treebase (http://purl.org/phylo/treebase/phylows/study/TB2:S17643).
The data sets supporting the results of the article are available in GenBank under accession numbers KR006351-KR006603. All of the phylogenetic sequence data in this study are deposited in GenBank (National Center for Biotechnology Information) with the link http://www.ncbi.nlm.nih.gov/nuccore/.
All additional materials supporting the results of the article are included as additional files.
We thank Dr. Yu-Min Shui for assistance in the field. This work was supported by the National Natural Science Foundation of China (Grants 31170198, 31200159 and31470333).
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