Figure 3
Degree and pattern of pectin methylesterification (DM) in cell wall extracted from spikes of 02-5B-318 R and Saragolla S plants. (a) The DM was quantified and expressed as methanol to uronic acid molecular ratio (%). Data represent the average ± standard deviation (n = 6). Asterisks indicate data sets significantly different between 02-5B-318R and SaragollaS according to Student’s t-test (p < 0.01). (b) Immunodot analysis of pectin extracted from spikes of 02-5B-318R and SaragollaS plants using LM7 antibody. The micrograms of chelating agent soluble solid fractions from the two genotypes applied to the nitrocellulose membrane were indicated. The experiments were repeated three times with similar results. R = 02-5B-318R; S = SaragollaS.