Independent and interactive effects of DOF affecting germination 1 (DAG1) and the Della proteins GA insensitive (GAI) and Repressor of ga1-3(RGA) in embryo development and seed germination
© Boccaccini et al. 2014
Received: 24 April 2014
Accepted: 16 July 2014
Published: 26 July 2014
The transcription factor DOF AFFECTING GERMINATION1 (DAG1) is a repressor of seed germination acting downstream of the master repressor PHYTOCROME INTERACTING FACTOR3-LIKE 5 (PIL5). Among others, PIL5 induces the expression of the genes encoding the two DELLA proteins GA INSENSITIVE 1 (GAI) and REPRESSOR OF ga1-3 (RGA).
Based on the properties of gai-t6 and rga28 mutant seeds, we show here that the absence of RGA severely increases dormancy, while lack of GAI only partially compensates RGA inactivation. In addition, the germination properties of the dag1rga28 double mutant are different from those of the dag1 and rga28 single mutants, suggesting that RGA and DAG1 act in independent branches of the PIL5-controlled germination pathway. Surprisingly, the dag1gai-t6 double mutant proved embryo-lethal, suggesting an unexpected involvement of (a possible complex between) DAG1 and GAI in embryo development.
Rather than overlapping functions as previously suggested, we show that RGA and GAI play distinct roles in seed germination, and that GAI interacts with DAG1 in embryo development.
KeywordsDAG1 GAI RGA Seed germination Embryogenesis Arabidopsis thaliana
Seed germination is controlled by multiple endogenous and environmental factors , which are integrated to trigger this developmental process at the right time. Two plant hormones play important roles in seed germination: gibberellins (GA), which have an inductive effect, and abscissic acid (ABA), which inhibits the process . Several physical factors affect seed germination, such as light, temperature and water potential. The effect of light is mediated mainly by the photoreceptor phytochrome B (phyB) , and the levels of GA and ABA are oppositely modulated by light, which induces GA biosynthesis and causes a reduction in ABA levels ,. Among the regulators involved in phyB-mediated GA-induced seed germination in Arabidopsis, the bHLH transcription factor PHYTOCHROME INTERACTING FACTOR 3-LIKE 5 (PIL5) represents the master repressor . In seeds kept in the darkness, PIL5 activates transcription of GA-INSENSITIVE (GAI) and REPRESSOR OF ga1-3 (RGA) , two nuclear-localized DELLA transcriptional regulators that repress GA-mediated responses and are rapidly degraded in response to GA –. Indeed, it has been shown that in Arabidopsis all DELLA proteins are under negative control by GA and the proteasome . Accordingly, gain-of-function della mutants show GA-insensitive phenotypes (i.e. dwarfism), whereas loss-of-function mutations result in GA-hypersensitive phenotypes (e.g. increased height) .
The DELLA proteins represent a subfamily of the GRAS plant transcription factors, and are characterized by the N-terminal DELLA domain. In Arabidopsis there are five DELLA genes: the above mentioned GAI and RGA, and RGA-LIKE 1,2,3 (RGL 1,2,3). An insertional mutagenesis approach enabled cloning of Arabidopsis GAI by isolation of a Ds transposon-mutated gai-t6 allele , while RGA was identified by loss-of-function mutations  and shown to encode a protein closely related to GAI . GAI and RGA were shown to have overlapping functions in repressing many growth processes, such as leaf expansion, stem elongation, floral initiation and seed germination ,. Moreover, double mutant seeds have a higher germination rate than the wild-type ones in response to increasing Red (R) light fluences .
As of other DELLA proteins involved in seed germination, RGL2 also plays a negative key role: genetic data clearly showed that only a combination of rga and rgl2 or gai-t6 and rgl2 mutant alleles could restore seed germination in a ga1-3 background .
We have previously shown that the DOF transcription factor DAG1 (DOF AFFECTING GERMINATION1) is a repressor of seed germination in Arabidopsis: dag1 knock-out mutant seeds require lower GA and R light fluence rates than wild-type seeds to germinate –. We have also pointed out that DAG1 acts in the phyB-mediated pathway: DAG1 expression is reduced in seeds irradiated for 24 hours with R light, and this reduction is dependent on PIL5; in pil5 mutant seeds DAG1 expression is reduced irrespective of light conditions, indicating that DAG1 acts downstream of PIL5; moreover, DAG1 negatively regulates GA biosynthesis by directly repressing the GA biosynthetic gene AtGA3ox1. Very recently, we demonstrated that GAI cooperates with DAG1 in repressing AtGA3ox1, and that it directly interacts with DAG1 .
In order to further clarify the role of DAG1 in phyB-mediated seed germination, we focus here on the functional relationship between DAG1, RGA and GAI in the control of this process. We provide genetic and phenotypic evidence suggesting different roles of the two DELLA proteins in seed germination and with respect to DAG1.
The gai-t6 and rga28mutant alleles show different seed germination phenotypes
It has been reported that concurrent inactivation of both GAI and RGA increases the seed germination potential: gai-t6rga28 double mutant seeds require less R light fluences than wild-type ones to germinate  - a phenotype that is reminiscent of dag1 mutant seeds, which need a fluence rate six times lower than wild-type to germinate . We compared the seed germination properties of stored (28 days after ripening, DAR) gai-t6, rga28, double mutant gai-t6rga28 and Col-0 wild-type seeds, under phyB-dependent germination conditions ,. We also assessed the germination properties under white light and in the dark, with or without stratification.
Under white light the only substantial difference in the germination rate of stratified seeds was observed at 24 hours between rga28 and gai-t6rga28 mutant seeds compared to wild-type ones (56%, 64% and 85% respectively), and in all cases 100% germination was attained in 72 hours (Figure 1B). In the absence of cold treatment, although all lines reached 100% germination after 96 hours, gai-t6 seeds germinated faster and rga28 seeds slower than wild-type, while gai-t6rga28 mutants showed the same germination kinetics of the latter, i.e. roughly 60%, 40% and 30%, respectively, after 24 hours (Figure 1C). After 5 days in the dark, stratified seeds of all mutant lines germinated completely as did wild-type seeds; on the contrary, without stratification, the germination rate of gai-t6 and wild-type seeds were similar (above 80%), whereas both rga28 and gai-t6rga28 seeds germinated significantly less (approximately 40%) (Figure 1D). As one function of stratification is to remove seed dormancy, we verified whether the rga28 germination phenotype was due to increased seed dormancy.
A seed germination assay, without stratification, was performed with freshly harvested mutant seeds, and with seeds respectively at 7, 14, 21 DAR, to asses a possible loss of dormancy due to seed storage. The germination rate was scored after seven days under white light. Freshly harvested and 7 DAR single gai-t6 and rga28 and double gai-t6rga28 mutant seeds showed a germination rate lower than 10%, similarly to wild-type seeds (8% germination). The germination of gai-t6 and wild-type seeds increased up to 48% and 52%, respectively, after two weeks of storage; dormancy was almost completely relieved after three weeks - 83% and 97% germination for gai-t6 and wild-type seeds, respectively. Conversely, rga28 and gai-t6rga28 14 DAR seeds still retained a significantly higher level of dormancy, as revealed by a germination rate of 30% and 22%, respectively. After three weeks of storage both rga28 and gai-t6rga28 mutant seeds lost part of their dormancy (38% and 53% germination, respectively), although only rga28 seeds showed a significant difference with wild-type seeds (97% germination) (Figure 1E).
These results point to different effects of GAI and RGA on seed dormancy: the absence of RGA severely increases dormancy, while lack of GAI partially compensates RGA inactivation, as gai-t6rga28 mutant seeds show a milder phenotye than the rga28 single mutant.
The dag1 and rga28mutations are not epistatic
To elucidate the genetic relationship between the DOF gene DAG1 and the DELLA–encoding genes RGA and GAI, we constructed the dag1rga28 double mutant. In contrast, attempts to isolate the dag1gai-t6 double mutant were unsuccessful (see below). As the dag1 and rga28 mutant lines are in different ecotypes (Ws-4 and Col-0, respectively), several lines for each genotype - double mutants, parental lines and wild-type - were selected and analysed in order to minimize the effect of the ecotype on the phenotype of interest.
Since the dag1rga28 seed germination phenotype is not completely similar to that of the single mutants, dag1 and rga28 do not have an epistatic relationship.
Simultaneous inactivation of both DAG1 and GAIaffects embryo development
Expression of DAG1complements embryo defects and germination properties
DAG1is expressed during embryo development
We had previously characterized the DAG1 transcription factor as a repressor of seed germination – that acts downstream of PIL5 and negatively regulates GA biosynthesis . As also the DELLA proteins RGA and GAI act downstream of PIL5 in seed germination , we investigated on the respective roles of these DELLA proteins in this process and their relationship with DAG1.
RGA and GAI have distinct roles in seed germination
RGA and GAI have been reported to be involved in several growth processes ,; however, the single null mutants rga24, rga28 and gai-t6 were reported to lack any visible phenotype, and a functional redundancy of the two proteins had been suggested ,,. As for seed germination, the single rga28 and gai-t6 mutants were shown to behave similarly to the wild-type in response to increasing red light fluences .
Here we show that the rga28 and gai-t6 single mutants have different seed germination phenotypes, suggesting (at least partially) distinct functions for RGA and GAI in this developmental process. In particular, rga28 seeds have, in the absence of stratification, a lower germination rate than wild-type irrespective of light conditions. This germination phenotype is likely due to an increased dormancy - as revealed by our germination assays on freshly harvested seeds and on seeds at different DAR.
Our data suggest that RGA plays a negative role in the regulation of seed dormancy. RGA has been shown to be involved in seed dormancy and to be directly activated by SPATULA (SPT), which also inhibits the negative regulator of RGA MOTHER OF-FT-AND-TFL1 (MFT) -, but dormancy of the rga28 single mutant was not analysed by those authors.
On the other hand, our work shows that although the gai-t6 single mutant does not have a dormancy phenotype, lack of GAI partially compensates RGA inactivation, as gai-t6rga28 mutant seeds show a milder phenotye than the rga28 single mutant. In addition, in our hands gai-t6 mutant seeds showed a germination potential slightly higher than wild-type under white light in the absence of stratification, similar to that of the dag1 mutant (; this work).
It should be pointed out that RGA and GAI also differ in their transcriptional regulation in connection with DAG1: while we have recently shown a reciprocal negative transcriptional control of the genes DAG1 and GAI during seed germination , a previous microarray analysis of ours showed that GAI, but not RGA, was upregulated by DAG1 inactivation .
Inactivation of GAI enhances the dag1embryo mutant phenotype
We have previously reported that dag1 siliques contain numerous aborted seeds . In this work, attempts to isolate the dag1gai-t6 double mutant were unsuccessful, suggesting that the simultaneous inactivation of both DAG1 and GAI results in an embryo-lethal phenotype, i.e. a more severe phenotype than inactivation of only DAG1. This is not due to an additive effect of the two mutations, since a statistical analysis of the siliques revealed that while dag1 contained 17% abnormal seeds, only 2% aborted seeds were present in gai-t6 and in wild-type siliques. Thus, the absence of GAI does not in itself lead to seed abnormalities, but inactivation of this gene in a dag1 mutant background is apparently responsible for embryo lethality. This may be an additional indication of the cooperation between DAG1 and GAI in controlling common target genes that we pointed out in a previous paper, where we showed that the two proteins cooperate in negatively regulating the AtGA3ox1 gene . Consistently, we could restore embryo development by expressing the DAG1-HA chimaeric protein in the dag1gai-t6 double mutant background.
The earliest phenotype of the dag1gai-t6 double mutant is an impairment in cell divisions in the basal portion of the globular stage embryo, the hypophyseal and the procambial precursor cells, but not in the ground precursor cells. Consistent with this mutant phenotype, the DAG1 promoter is active in the embryo starting from the globular stage.
Simultaneous inactivation of POLTERGEIST (POL) and POLTERGEIST-LIKE 1 (PLL1) results in defects in basal embryo patterning similar to what described here for the dag1gai-t6 double mutant . POL and PLL1 are two related phosphatases required to establish the vascular axis in the embryo, by inducing expression of the WUSCHEL (WUS) homolog WUSCHEL RELATED HOMEOBOX 5 (WOX5).
It is tempting to speculate that DAG1 and GAI may also function in this molecular network. As the double mutant dag1gai-t6 has a more severe phenotype than the double mutant polpll1, one might hypothesize that DAG1 and GAI act upstream of POL and PLL1. Further analysis on the functional and molecular relationship among these factors will help unveiling the complex signaling underlying embryo development.
Here we show that the DELLA proteins RGA and GAI have, at least partially, different roles in the seed germination process. Indeed, RGA inactivation results in increased seed dormancy, whereas lack of GAI partially compensates this phenotype, as gai-t6rga28 mutant seeds show a milder phenotye than the rga28 single mutant.
With respect to DAG1, our data suggest that this latter and RGA act in independent branches of the PIL5-controlled germination pathway, whereas GAI and DAG1 are involved in embryo development since the dag1gai-t6 double mutant proved embryo-lethal. This latter finding should be regarded in the context of the cooperation of DAG1 and GAI in regulating common target genes, such as in the case of the GA biosynthetic gene AtGA3ox1 that we have very recently demonstrated .
Plant material and growth conditions
dag1 is the allele described in Papi et al. in Ws-4 ecotype. The rga28, gai-t6 and gai-t6rga28 (Col-0) mutants, kindly provided by Dr. G. Choi, are described by Oh et al.. dag1rga28 was obtained by crossing the single mutants, and identified in the F3 generation by PCR analysis. The gai-t6 and dag1 mutants were crossed using both lines as female parent. F1 plants derived from the cross were analysed by PCR to confirm the presence of the mutant alleles in heterozygosis. As the single mutants were in different ecotypes, the parental lines (dag1, rga28, gai-t6) and the wild-type were also selected from the cross. Several lines for each genotype were selected and analysed in order to minimize the effect of the two different ecotypes on the phenotypes of interest. The rga24 and gai-t6 mutant lines in Ler ecotype  were from the ABRC stock.
The dag1gai-t6DAG1-HA lines were isolated from the F2 generation derived from the cross gai-t6 × dag1DAG1-HA, by PCR-based genotyping.
All Arabidopsis thaliana lines used in this work were grown in a growth chamber at 24/21% C with 16/8-h day/night cycles and light intensity of 300 μmol/m-2 s−1 as previously described ,. All the primers used for the screenings are listed in Additional file 2: Table S1.
Seed germination assays
All seeds used for germination tests were harvested from mature plants grown at the same time, in the same conditions, and stored for the same time (7, 14, 21, 28 DAR) under the same conditions, except where freshly harvested seeds were used. Germination assays were performed according to Gabriele et al. . For phyB-dependent germination experiments, seeds, with or without cold treatment (stratification, 2 days at 4°C), were exposed to a pulse of FR light (40 μmol m−2 s−1), then a pulse of R light (90 μmol m−2 s−1) and subsequently kept in the dark for 5 days: under these conditions germination is mediated only by phyB. For the germination assays in the dark, seeds were exposed to a pulse of white light, then kept in the dark for 5 days. All germination assays were repeated with three seed batches, and one representative experiment is shown. Bars represent the mean ± SEM of three biological repeats (25 seeds per biological repeat). P values were obtained from a Student’s unpaired two-tail t test comparing the mutant with its control (* = p ≤ 0,05 ** = p ≤ 0,01).
Cytology and microscopy
For staining of ovules and seeds, siliques were harvested and slit open on one side. Tissue was fixed in 50% methanol/10% acetic acid and then subjected to 3 h treatment of 1% SDS and 0.2 N NaOH at room temperature. Siliques were rinsed in water, incubated in 25% bleach solution (2.5% active Cl−) for 1 to 5 min, rinsed again, and then transferred to 1% periodic acid. The samples were then further processed as described before .
For confocal microscopy, a LSM 710 (Zeiss) spectral confocal laser-scanning microscope was used. Excitation wavelengths for propidium iodide-stained samples was 488 nm. Data were processed for some two-dimensional orthogonal sections, 3D rendering, using the open source software Osirix (; http://www.osirix-viewer.com/AboutOsiriX.html) on a quadxeon 2.66-Ghz, 2-GB RAM Apple Mac pro workstation.
Analysis of defective embryos of the F1 plants derived from the cross dag1 × gai-t6. was performed under an Axioskop 2 plus microscope (Zeiss). Bars represent the average of about one hundred mature siliques, error bars represents SD. P values were obtained from a Student’s unpaired two-tail t test comparing the mutant with its control (* = p ≤ 0,05 ** = p ≤ 0,01).
GUS constructs and analysis
The DAG1:GUS line is the one described in Gualberti et al.. Histochemical staining and microscopic analysis were carried out according to Blazquez et al.. Stained embryos (after washing in 70% ethanol) were analysed and photographed under an Axioskop 2 plus microscope (Zeiss).
We thank G. Choi who kindly provided the rga28, gai-t6, gai-t6rga28 mutant lines. This work was partially supported by research grants from Ministero dell’Istruzione, Università e Ricerca, Progetti di Ricerca di Interesse Nazionale, and from Sapienza Università di Roma to PC, and from Istituto Pasteur Fondazione Cenci Bolognetti to PV.
- Koornneef M, Karssen CM: Seed Dormancy and Germination in Arabidopsis. Cold Spring Harbor, Cold Spring Harbor Laboratory Press 1994.Google Scholar
- Koornneef M, Bentsink L, Hilhorst H: Seed dormancy and germination. Curr. Opin. Plant Biol. 2002, 5: 33-36. 10.1016/S1369-5266(01)00219-9.View ArticlePubMedGoogle Scholar
- Shinomura T, Nagatani A, Chory J, Furuya M: The induction of seed germination in Arabidopsis thaliana is regulated principally by phytochrome B and secondarily by phytochrome A. Plant Physiol. 1994, 104: 363-371.PubMed CentralPubMedGoogle Scholar
- Yamaguchi S, Smith MW, Brown RGS, Kamiya Y, Sun TP: Phytochrome regulation and differential expression of gibberellin 3β-hydroxylase genes in germinating Arabidopsis seeds. Plant Cell. 1998, 10: 2115-2126.PubMed CentralPubMedGoogle Scholar
- Seo M, Hanada A, Kuwahara A, Endo A, Okamoto M, Yamauchi Y, North H, Marion-Poll A, Sun TP, Koshiba T, Koshiba T, Kamiya Y, Yamaguchi S, Nambara E: Regulation of hormone metabolism in Arabidopsis seeds: phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism. Plant J. 2006, 48: 354-366. 10.1111/j.1365-313X.2006.02881.x.View ArticlePubMedGoogle Scholar
- Oh E, Kim J, Park E, Kim JI, Kang C, Choi G: PIL5, a phytochrome-interacting basic helix–loop–helix protein, is a key negative regulator of seed germination in Arabidopsis thaliana. Plant Cell. 2004, 16: 3045-3058. 10.1105/tpc.104.025163.PubMed CentralView ArticlePubMedGoogle Scholar
- Oh E, Yamaguchi S, Hu J, Yusuke J, Jung B, Paik I, Lee HS, Sun TP, Kamiya Y, Choi G: PIL5, a phytochrome-interacting bHLH protein, regulates gibberellin responsiveness by binding directly to the GAI and RGA promoters in Arabidopsis seeds. Plant Cell. 2007, 19: 1192-1208. 10.1105/tpc.107.050153.PubMed CentralView ArticlePubMedGoogle Scholar
- Tyler L, Thomas SG, Hu J, Dill A, Alonso JM, Ecker JR, Sun TP: DELLA proteins and gibberellin-regulated seed germination and floral development in Arabidopsis. Plant Physiol. 2004, 135: 1008-1019. 10.1104/pp.104.039578.PubMed CentralView ArticlePubMedGoogle Scholar
- Fleet CM, Sun TP: A DELLAcate balance: the role of gibberellin in plant morphogenesis. Curr Opin Plant Biol. 2005, 8: 77-85. 10.1016/j.pbi.2004.11.015.View ArticlePubMedGoogle Scholar
- Sun TP: The molecular mechanism and evolution of the GA-GID1-DELLA signaling module in plants. Curr Biol. 2011, 21: 338-345. 10.1016/j.cub.2011.02.036.View ArticleGoogle Scholar
- Feng S, Martinez C, Gusmaroli G, Wang Y, Zhou J, Wang F, Chen L, Yu L, Iglesias-Pedraz JM, Kircher S, Schäfer E, Fu X, Fan LM, Deng XW: Coordinated regulation of Arabidopsis thaliana development by light and gibberellins. Nature. 2008, 451: 475-479. 10.1038/nature06448.PubMed CentralView ArticlePubMedGoogle Scholar
- Ariizumi T, Hauvermale A, Nelson SK, Hanada A, Yamaguchi S, Steber CM: Lifting DELLA Repression of Arabidopsis Seed Germination by Nonproteolytic Gibberellin Signaling. Plant Physiol. 2013, 162: 2125-2139. 10.1104/pp.113.219451.PubMed CentralView ArticlePubMedGoogle Scholar
- Peng J, Carol P, Richards DE, King KE, Cowling RJ, Murphy GP, Harberd NP: The Arabidopsis GAI gene defines a signalling pathway that negatively regulates gibberellin responses. Genes Dev. 1997, 116: 3194-3205. 10.1101/gad.11.23.3194.View ArticleGoogle Scholar
- Silverstone AL, Mak PY, Martínez EC, Sun TP: The new RGA locus encodes a negative regulator of gibberellin response in Arabidopsis thaliana. Genetics. 1997, 146: 1087-1099.PubMed CentralPubMedGoogle Scholar
- Silverstone AL, Ciampaglio CN, Sun TP: The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway. Plant Cell. 1998, 10 (2): 155-169. 10.1105/tpc.10.2.155.PubMed CentralView ArticlePubMedGoogle Scholar
- Dill A, Sun TP: Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana. Genetics. 2001, 159: 777-785.PubMed CentralPubMedGoogle Scholar
- King K, Moritz T, Harberd N: Gibberellins are not required for normal stem growth in Arabidopsis thaliana in the absence of GAI and RGA. Genetics. 2001, 1596: 767-776.Google Scholar
- Cao D, Hussain A, Cheng H, Peng J: Loss of function of four DELLA genes leads to light- and gibberellin-independent seed germination in Arabidopsis. Planta. 2005, 2236: 105-113. 10.1007/s00425-005-0057-3.View ArticleGoogle Scholar
- Papi M, Sabatini S, Bouchez D, Camilleri C, Costantino P, Vittorioso P: Identification and disruption of an Arabidopsis zinc finger gene controlling seed germination. Genes Dev. 2000, 14: 28-33.PubMed CentralPubMedGoogle Scholar
- Papi M, Sabatini S, Altamura MM, Henning L, Schafer E, Costantino P, Vittorioso P: Inactivation of the phloem-specific DOF zinc finger gene DAG1 affects response to light and integrity of the testa of Arabidopsis seeds. Plant Physiol. 2002, 128: 411-417. 10.1104/pp.010488.PubMed CentralView ArticlePubMedGoogle Scholar
- Gualberti G, Papi M, Bellucci L, Ricci I, Bouchez D, Camilleri C, Costantino P, Vittorioso P: Mutations in the DOF zinc finger genes DAG1 and DAG2 influence with opposite effects the germination of Arabidopsis seeds. Plant Cell. 2002, 14: 1253-1263. 10.1105/tpc.010491.PubMed CentralView ArticlePubMedGoogle Scholar
- Gabriele S, Rizza A, Martone J, Circelli P, Costantino P, Vittorioso P: The DOF protein DAG1 mediates PIL5 activity on seed germination by negatively regulating the GA biosynthetic gene AtGA3ox1. Plant J. 2010, 61: 312-323. 10.1111/j.1365-313X.2009.04055.x.View ArticlePubMedGoogle Scholar
- Boccaccini A, Santopolo S, Capauto D, Lorrai R, Minutello E, Serino G, Costantino P, Vittorioso P: The DOF protein DAG1 and the DELLA protein GAI cooperate in negatively regulating AtGA3ox1 gene. Molecular plant 2014, in press.,Google Scholar
- Penfield S, Josse EM, Kannangara R, Gilday AD, Halliday KJ, Graham IA: Cold and light control seed germination through the bHLH transcription factor SPATULA. Curr. Biol. 2005, 15: 1998-2006. 10.1016/j.cub.2005.11.010.View ArticlePubMedGoogle Scholar
- Penfield S, Gilday AD, Halliday KJ, Graham IA: DELLA-mediated cotyledon expansion breaks coat-imposed seed dormancy. Curr Biol. 2006, 16 (23): 2366-2370. 10.1016/j.cub.2006.10.057.View ArticlePubMedGoogle Scholar
- Vaistij FE, Gan Y, Penfield S, Gilday AD, Dave A, He Z, Josse EM, Choi G, Halliday KJ, Graham IA: Differential control of seed primary dormancy in Arabidopsis ecotypes by the transcription factor SPATULA Proc Natl Acad Sci U S A. 2013, 110: 10866-10871.PubMedGoogle Scholar
- Rizza A, Boccaccini A, Lopez-Vidriero I, Costantino P, Vittorioso P: Inactivation of the ELIP1 and ELIP2 genes affects Arabidopsis seed germination. New Phytol. 2011, 190: 896-905. 10.1111/j.1469-8137.2010.03637.x.View ArticlePubMedGoogle Scholar
- Song S-K, Hofhuis H, Min Lee M, Clark SE: Key divisions in the early Arabidopsis embryo require POL and PLL1 phosphatases to establish the root stem cell organizer and vascular axis. Develop. Cell. 2008, 15: 98-109. 10.1016/j.devcel.2008.05.008.View ArticleGoogle Scholar
- Truernit E, Bauby H, Dubreucq B, Grandjean O, Runions J, Barthélémy J, Palauqui JC: High-resolution whole-mount imaging of three-dimensional tissue organization and gene expression enables the study of Phloem development and structure in Arabidopsis. Plant Cell. 2008, 20: 1494-1503. 10.1105/tpc.107.056069.PubMed CentralView ArticlePubMedGoogle Scholar
- Rosset A, Spadola L, Ratib O: OsiriX: an open-source software for navigating in multidimensional DICOM images. J Digit Imaging. 2004, 17: 205-216. 10.1007/s10278-004-1014-6.PubMed CentralView ArticlePubMedGoogle Scholar
- Blázquez MA, Soowal LN, Lee I, Weigel D: LEAFY expression and flower initiation in Arabidopsis. Development. 1997, 124: 3835-3844.PubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The CreativeCommons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.