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Table 4 Quantification of identified peptides by Q-TOF LC-MSE in the pollen of B. pendula 'Youngii'.

From: Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen

Fragment I*1 III IV V VII VIII*1 X *2 XVII Direct coverage estimate Indirect coverage estimate Subfamily Direct estimate
Isoform Gene             
01A01 1A Ia: n.q. IIIa: 51 IVa: 100 Va: 46 VIIa: 75 VIIIa: n.q. Xa+g+c: 82 XVIIa: 100 - 4–41% 01 68–75%
01A06 1A Ia: n.q. IIIa: 51 IVa: 100 Vb: 23 VIIa: 75 VIIIa: n.q. Xa+g+c: 82 XVIIa: 100 - 4–5%   
01B01 1B Ib: 69 IIIb: 19 IVa: 100 Vb: 23 VIIa: 75 VIIIc: n.q. Xb: 18 XVIIa: 100 18–19% -   
01C04/01C05 1C Id: 31 IIIa: 51 IVa: 100 Va: 46 VIIa: 75 VIIId: 100 Xa+g+c: 82 XVIIa: 100 - 6%   
01D01 1D Ie: 0 IIIa: 51 IVa: 100 Vc: 0 VIIc: 0 VIIIe: 0 Xa+g+c: 82: XVIIa: 100 0% -   
02A01/02B01 2A Ia: n.q. IIIe: 13 IVa: 100 Ve: 32 VIIk: 25 VIIIk: n.q. Xa+g+c: 82 XVIIa: 100 13% - 02 25–32%
02C01 2C Ia: n.q. IIIf: 17 IVa: 100 Ve: 32 VIIk: 25 VIIIk: n.q. Xa+g+c: 82 XVIIa: 100 17% -   
  1. Numbers indicate the relative amount of fragment variants compared to the total amount of homologues fragments. Amounts were averaged over the two duplicates. Note that quantification was not possible for all peptide variants1,2 and that the displayed abundances indicate the relative amounts among those variants that could be quantified. n.q. = not possible to quantify.
  2. * 1 Quantification was not possible for all the peptide variants, because Ia (1854,91 Da) and VIIIa (1854,89 Da), and Ij (1840,89 Da) and VIIIc (1840,88 Da) had a similar mass. Fragment VIIIk overlaps with a keratin peptide.
  3. * 2 Fragments Xa and Xg have exactly the same mass and cannot be distinguished. The peptide peak of Xc overlaps with the first isotope peak of peptide Xa = g because they differ exactly 1Da in size and have the same charge. Xc cannot be identified as a result.