Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen

BMC Plant Biology

Table 4 Quantification of identified peptides by Q-TOF LC-MS^E in the pollen of B. pendula 'Youngii'.

Fragment		I*¹	III	IV	V	VII	VIII*¹	X *²	XVII	Direct coverage estimate	Indirect coverage estimate	Subfamily	Direct estimate
Isoform	Gene
01A01	1A	Ia: n.q.	IIIa: 51	IVa: 100	Va: 46	VIIa: 75	VIIIa: n.q.	Xa+g+c: 82	XVIIa: 100	-	4–41%	01	68–75%
01A06	1A	Ia: n.q.	IIIa: 51	IVa: 100	Vb: 23	VIIa: 75	VIIIa: n.q.	Xa+g+c: 82	XVIIa: 100	-	4–5%
01B01	1B	Ib: 69	IIIb: 19	IVa: 100	Vb: 23	VIIa: 75	VIIIc: n.q.	Xb: 18	XVIIa: 100	18–19%	-
01C04/01C05	1C	Id: 31	IIIa: 51	IVa: 100	Va: 46	VIIa: 75	VIIId: 100	Xa+g+c: 82	XVIIa: 100	-	6%
01D01	1D	Ie: 0	IIIa: 51	IVa: 100	Vc: 0	VIIc: 0	VIIIe: 0	Xa+g+c: 82:	XVIIa: 100	0%	-
02A01/02B01	2A	Ia: n.q.	IIIe: 13	IVa: 100	Ve: 32	VIIk: 25	VIIIk: n.q.	Xa+g+c: 82	XVIIa: 100	13%	-	02	25–32%
02C01	2C	Ia: n.q.	IIIf: 17	IVa: 100	Ve: 32	VIIk: 25	VIIIk: n.q.	Xa+g+c: 82	XVIIa: 100	17%	-

Numbers indicate the relative amount of fragment variants compared to the total amount of homologues fragments. Amounts were averaged over the two duplicates. Note that quantification was not possible for all peptide variants^1,2 and that the displayed abundances indicate the relative amounts among those variants that could be quantified. n.q. = not possible to quantify.
* 1 Quantification was not possible for all the peptide variants, because I_a (1854,91 Da) and VIII_a (1854,89 Da), and I_j (1840,89 Da) and VIII_c (1840,88 Da) had a similar mass. Fragment VIII_k overlaps with a keratin peptide.
* 2 Fragments X_a and X_g have exactly the same mass and cannot be distinguished. The peptide peak of X_c overlaps with the first isotope peak of peptide X_{a = g} because they differ exactly 1Da in size and have the same charge. X_c cannot be identified as a result.

ISSN: 1471-2229