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Table 1 Number of identified PR-10 sequences in nine birch species.

From: Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen

Species Number of
sequenced
clones
Subfamily 01 Subfamily 02 Subfamily 03 Subfamily 04 Subfamily 05 Subfamily 06 Subfamily 07 Total
   Seqs Genes Seqs Genes Seqs Genes Seqs Genes Seqs Genes Seqs Genes Seqs Genes Seqs Genes
Subgenus Betulaster:                  
B. nigra 155 10 6 4 3 7 4 2 1 2 1 - - - - 25 15
Subgenus Neurobetula:                  
B. chichibuensis 170 5 4 3 2 10 7 1 1 1 1 2 2 - - 22 17
B. schmidtii 184 3 2 3 2 1 1 1 1 1 1 2 2 1 1 12 10
Subgenus Betulenta:                  
B. lenta 106 3 2 3 2 4 4 1 1 2 1 - - 1 1 14 11
Subgenus Betula:                  
B. costata 103 9 8 3 2 5 5 1 1 2 1 - - - - 20 17
B. pendula 102 5 3 2 2 5 4 2 1 2 1 - - - - 16 11
B. plathyphylla 103 6 4 4 3 6 3 2 1 2 1 - - - - 20 12
B. populifolia 106 4 4 4 2 5 3 2 1 2 1 - - - - 17 11
B. pendula reference * 2 - - 4 - 3 - 4 - 1 - 1 - - - - - 13
  1. The number of clones sequenced in both directions and the number of identified sequences and genes are shown per species.1 Subfamily 01 to 05 were previously identified [32], while subfamily 06 and 07 are new. Homology to mRNA sequences suggests that subfamily 01 and 02 are expressed in pollen.
  2. *1 Species were diploid (2n) as measured by flow cytometry. The identification of alleles of a single gene is based on the criterion of having > 98.5% sequence similarity, and by allowing maximally two alleles per gene.
  3. *2 Genes identified in B. pendula [32].