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Table 1 Amino acid residues that directly bind the ADPglucose substrate in AgtGS and their corresponding invariant residues in plant SS proteins

From: Cloning, characterisation and comparative analysis of a starch synthase IV gene in wheat: functional and evolutionary implications

 

SSs: Group A

 

SSs: Group B

AgtGS

GBSSI

SSI

SSII

SSIII

SSIV

Lys15

Lys91

Lys153

Lys322

Lys1194

Lys435

Gly18

Gly94

Gly156

Gly325

Gly1197

Gly438

Asp138

Asp229

Asp285

Asp447

Asp1309

Asp558

His163

His258

His314

His476

His1337

His586

Asn246

Asn347

Asn408

Asn562

Asn1385

Asn671

Ile297

Val/Ile400

Ile460

Ile619

Val/Ile1437

Val/Ile724

Arg299

Arg402

Arg462

Arg621

Arg1439

Arg726

Gly327

Gly431

Gly490

Gly649

Gly1467

Gly754

Tyr354

Phe458

Phe517

Phe676

Phe/Tyr1499

Tyr784

Asn355

Ser/Asn459

Ser/Asn518

Ser677

Asp1500

Ap785

  1. Amino acids that are part of the catalytic site in AgtGS were identified in previous studies [18, 19, 43, 44] and the equivalent residues in plant SSs were identified after alignment of AgtGS using ClustalW [34]. Only AgtGS amino acids that were conserved across all of the SS isoforms are shown. Data was extracted from the alignment shown in Figure 8.