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Table 3 Caffeoylquinic acids content in leaves of globe artichoke, cultivated and wild cardoon and expression level of HCT in the different plant accessions

From: Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L

 

Globe artichoke 'Romanesco C3'

Globe artichoke 'Violet Margot'

Cultivated cardoon

Wild cardoon

Caffeoylquinic acid (1)*

0.21 ± 0.06 c

2.01 ± 0.03 a

0.61 ± 0.07 b

1.83 ± 0.03 a

Chlorogenic acid (5-CQA) (2)

13.49 ± 2.73 bc

32.74 ± 0.79 a

9.32 ± 1.18 c

19.02 ± 0.97 b

p -Coumaroylquinic acid (3)

0.28 ± 0.03 ab

trace

0.18 ± 0.03 b

0.35 ± 0.03 a

Feruloylquinic acid (4)

0.96 ± 0.32 a

0.79 ± 0.06 a

0.13 ± 0.00 c

0.34 ± 0.11 b

Dicaffeoilquinic acids (5,6)

1.33 ± 0.66 d

26.03 ± 1.09 b

7.16 ± 1.30 c

39.04 ± 3.45 a

Total Caffeoylquinic acids (1,2,5,6)

15.03 ± 3.10

60.78 ± 1.98

17.09 ± 2.59

59.89 ± 4.30

HCT expression

+/-

+

+/-

+

  1. Caffeoylquinic concentrations are expressed in mg/g dry matter. Within a column, means with the letter are not significantly different (P < 0.01; Tukey's HSD test).
  2. HCT expression was measured by northern blot analysis achieved with HCT probes specifically designed for each plant accession. +/- indicates a barely detectable signal, + indicates a strong signal.
  3. * Numbers in brackets refer to peaks reported in Fig. 6