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Table 2 Kinetic parameters of recombinant HCT

From: Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L

Varying substrate

Saturating substrate

kinetic parameters

  

Km(μM)

Vmax(nkat/mg)

Vmax/Km(nkat/mg/μM)

quinate

p-coumaroyl-CoA

9.2 × 103 ± 5.6 × 103

2.1 ± 1.2

0.0002

p-coumaroyl-CoA

quinate

53.0 ± 13.0

2.2 ± 0.5

0.041

p-coumaroyl-CoA

shikimate

701.67 ± 52

2.06 ± 0.55

0.003

Caffeoyl-CoA

quinate

61.75 ± 0.004

0.67 ± 0.04

0.01

  1. The Km and Vmax values were calculated from triplicates by the Lineweaver-Burk method.
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BMC Plant Biology

ISSN: 1471-2229

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