Background
RAPD (random amplified polymorphic DNA) analysis is wide used for the genetic mapping, taxonomic and phylogenetic studies of many organisms. It can be also applicable for the detection of DNA alterations after influence of many genotoxical agents as well [1]. UV-radiation can produce several major types of DNA lesions such as cyclobutane-type pirimidine dimers and the 6-4 photoproducts [2]. Another important types of DNA damages such as protein cross-links, DNA strand breaks, deletion or insertion of base pairs can also be induced by UV-irradiation. Whereas, irradiation by γ-rays leads to the increasing level of DNA break formation. These different types of DNA damages must be detected by changes in RAPD profiles. The aim of present study was to investigate the possibility mutation changes in plant DNA after the influence of UV-B and γ-ray with using RAPD method. The 10-mer primers with single nucleotide substitutions were used in order to estimate these structural alterations in DNA following after genotoxical agents' treatment.