Nitric oxide, a signaling molecule in plant cell reactivation
© The Author(s) 2005
Published: 31 May 2005
Nitric oxide is known to act as a biological messenger in divers signal transduction pathways in animal organisms. Initial investigations suggest that plants use nitric oxide as a signaling molecule via pathways remarkably similar to those found in mammals. Especially, the siganiling role of NO during plant defense reactions is well established. However, mounting evidences support the hypothesis that NO is a more general effector of plant growth and development.
In addition to cell cycle progression, the effect of the above drugs on the auxin-induced formation of embryogenic competent cells from leaf protoplasts (for more details see [1, 2]) have been followed. The promotive effect of SNP and the inhibitory effect of L-NMMA have been observed on the process, especially at low exogenous auxin (0.02 μM 2,4-dichlorophenoxyacetic acid) supplementation.
The obtained experimental data indicated that NO is required for and can stimulate auxin-mediated activation of cell division as well as embryogenic cell formation from differentiated leaf cells (protoplasts), but is not involved in the regulation of cell cycle progression of continuously dividing cells.
The presented work was partially supported by the national grant OTKA T34818.
- Feher A, Pasternak TP, Dudits D: Transition of somatic plant cells to an embryogenic state. Plant Cell Tissue and Organ Culture. 2003, 74: 201-228. 10.1023/A:1024033216561.View ArticleGoogle Scholar
- Pasternak T, Prinsen E, Ayaydin F, Miskolczi P, Potters G, Asard H, Van Onckelen H, Dudits D, Fehér A: The role of auxin, pH and stress in the activation of embryogenic cell division in leaf protoplast-derived cells of alfalfa (Medicago sativa L.). Plant Physiol. 2002, 129: 1807-1819. 10.1104/pp.000810.PubMed CentralView ArticlePubMedGoogle Scholar