Table 3 Sequences of microsatellite primer pairs for each locus, linkage group (LG) in M. truncatula and reference. Sequences are given in the 5' to 3' direction (H for T. Huguet, unpubl.; S for S. Santoni, unpubl.)

AFct32

TTTTTGTCCCACCTCATTAG

TTGGTTAGATTCAAAGGGTTAC

[35]

AFct45

TAAAAAACGGAAAGAGTTGGTTAG

GCCATCTTTTCTTTTGCTTC

[35]

MTLEC2A

CGGAAAGATTCTTGAATAGATG

TGGTTCGCTGTTCTCATG

3

[35]

MAW208206

CTTATGTGCGTGGTATTTCC

AGCTAAACCAACTACCTTTG

4

S

MAA660456

GGGTTTTTGATCCAGATCTT

AAGGTGGTCATACGAGCTCC

4

[34]

MAA660870

GTACATCAACAACTTTCTCCT

ATCAACAAAATTCATCGAAC

S

MAL368684

GACCCTAATAACCAACTCCA

CCGTTACCGTCACTGTCGT

1

S

FMT13

GATGAGAAAATGAAAAGAAC

CAAAAACTCACTCTAACACAC

1

S

MTR58

GAAGTGGAAATGGGAAACC

GAGTGAGTGAGTGTAAGAGTGC

1

[34]

MAL369471

ATTCACACAAACCCATCTTC

AAACCCTTAGCACCGACA

1

S

AI974357

ATCTCAATTCCCCAACTTGC

TCTCCTTCACCCATCTTTGC

3

H

B14B03

GCTTGTTCTTCTTCAAGCTC

ACCTGACTTGTGTTTTATGC

5

H

B21E13

GCCGATGGTACTAATGTAGG

AAATCTTGCTTGCTTCTCAG

2

H

E318681

ACCATCAACACCAACAGCAG

TGCTACTTCCGCTTTGTTCA

5

H

ENOD20

CGAACTTCGAATTACCAAAGTCT

TTGAGTAGCTTTTGGGTTGTC

8

H

MTIC7

ACCACTTCTCCATCCATCCA

AGCTTGCTGCATGAGTGCT

H

MTIC12

TTCCTCTTTTGACTCATCACCA

CAACAACATGTTCATGCTTAGAAAC

2

H

MTIC14

CAAACAAACAACACAAACATGG

CCCATTGATTGGTCAAGGTT

6

H

MTIC19

TCTAGAAAAAGCAATGATGTGAGA

TGCAACAGAAGAAGCAAAACA

2

H

MTIC21

GGTGATTGACTGTGGTGTCG

ATCCGGTCTCCCAGGTTCTA

2

H

MTIC27

CGATCGGAACGAGGACTTTA

CCCCGTTTTTCTTCTCTCCT

2

H

MTIC35

GAAGAAGAAAAAGAGATAGATCTGTGG

GGCAGGAACAGATCCTTGAA

7

H

MTIC48

TTTTTGTTAGTTTGATTTTAGGTG

GCTACAAAGTCTTCTTCCACA

5

H

MTIC51

AGTATAGTGATGAAGTGGTAGTGAACA

ACAAAAACTCTCCCGGCTTT

3

H

MTIC58

CATCATTAACAACAACGGCAAT

TGCAAACACAGAACCGAAGA

5

H

MTIC64

CCCGTTCTTTTATGTTGTGG

AACAAACACAATGGCATGGA

1

H

MTIC77

TCTTCATCGCTTTCTTCTATTTCA

GCCGTATGGTGTTGTTGATG

3

H

MTIC79

AAAATCCAAAGCCCTATCACA

AGCGTGAGATTTTTCCATCG

5

H

MTIC82

CACTTTCCACACTCAAACCA

GAGAGGATTTCGGTGATGT

7

H

MTIC84

TCTGAGAGAGAGACAAACAAAACAA

GGGAAAAGGTGTAGCCATTG

1

H

MTIC90

TTTTCTTCTTCAAACCCCTAACC

GATTATCGTTGAGCGGTGGT

4

H

MTIC93

AGCAGGATTTGGGACAGTTG

TACCGTAGCTCCCTTTTCCA

6

H

MTIC94

GCTACAACAGCGCTACATCG

CAGGGTCAGAGCAACAATCA

H

MTIC95

AAAGGTGTTGGGTTTTGTGG

AGGAAGGAGAGGGACGAAAG

H

MTIC103

TGGGTTGTCCTTCTTTTTGG

GGGTGCAGAAGTTTGACCA

8

H

MTIC107

CAAACCATTTCCTCCATTGTG

TACGTAGCCCCTTGCTCATT

1

H

MTIC124

TTGTCACGAGTGTTGGAATTTT

TTGGGTTGTCAATAATGCTCA

3

H

MTIC131

AAGCTGTATTTCTGATACCAAAC

CGGGTATTCCTCTTCCTCCA

3

H

MTIC134

GCAGTTCGCTGAGGACTTG

CAATTAGAGTCTACAGCCAAAAACT

6

H

MTIC135

GCTGACTGGACGGATCTGAG

CCAAAGCATAAGCATTCATTCA

8

H

MTIC145

CCAAAAGGGGCAATTTTCTT

GCATAATTCAATACTTGATCCATTTC

2

H

MTIC153

TCACAACTATGCAACAAAAGTGG

TGGGTCGGTGAATTTTCTGT

6

H

MTIC169

TCAAAACCCTAAAACCCTTTCTC

GCGTGCTAGGTTTGAGAGGA

3

H

MTIC183

AAATGGAAGAAAGTGTCACG

TTCTCTTCAAGTGGGAGGTA

7

H

MTIC185

AGATTTCAATTCTCAACAACC

TCTATGATGGATACGATACGG

8

H

MTIC188

GGCGGTGAAGAAGTAAACGA

AATCGGAGAAACACGAGCAC

8

H

MTIC189

CAAACCCTTTTCAATTTCAACC

ATGTTGGTGGATCCTTCTGC

3

H

MTIC210

CCAAACTGGCTGTGTTCAAA

GCGGTAAGCCTTGCTGTATG

2

H

MTIC230

GTAAGCGCCTGCTTGGACT

GAGATTCTGCCAAAATGCAA

2

H

MTIC232

TAAGAAAGCAGGTCAGGATG

TCCACAAATGTCTAAAACCA

7

H

MTIC233

GCGTAACGTAACAACATTCA

AAGGAACAATCCCAGTTTTT

1

H

MTIC237

CCCATATGCAACAGACCTTA

TGGTGAAGATTCTGTTGTTG

3

H

MTIC238

TTCTTCTTCTAGGAATTTGGAG

CCTTAGCCAAGCAAGTAAAA

5

H

MTIC247

TTCGCAGAACCTAAATTCAT

TGAGAGCATTGATTTTTGTG

1

H

MTIC248

TATCTCCCTTCTCCTTCTCC

GGATTGTGATGAAGAAATGG

8

H

MTIC249

TAGGTCATGGCTATTGCTTC

GTGGGTGAGGATGTGTGTAT

H

MTIC250

GCCTGAACTATTGTGAATGG

CGTTGATGATGTTCTTGATG

6

H

MTIC251

GCGATGCTATTGAGAAAACT

AAATAAACCCAAAGGACTCG

8

H

MTIC258

CACCACCTTCACCTAAGAAA

TGAAATTCACATCAACTGGA

1

H

MTIC272

AGGTGGATGGAGAGAGTCA

TCATGAATAGTGGCACTCAA

3

H

MTIC273

TGTTAGCAACTTTGTGATGG

TCCATTACAATACCCAGAGG

7

H

MTIC278

CTTACCCTCCACTGCTACTG

CGCATATAACAGAGGGTTTC

2

H

MTIC289

GCTGGTGTCAAAGAGGTCTA

AGATTTGAAACTGCCCTACA

H

MTIC299

AGGCTGTTGTTACACCTTTG

TCAAATGCTTAAATGACAAAT

8

H

MTIC304

TTGGGCTTAATTTGACTGAT

AGCGTAAAGTAAAACCCTTTC

2

H

MTIC314

TCTAATCCCAACAACACTCTT

GAAGAAGAAGCCATAGTGTGA

1

H

MTIC318

TCAACCAACTCAATGCCACT

TTGTTGTGAAATGGAAAATGG

7

H

MTIC326

GATCACCCTTTATGGAGTTTGAA

CGACTTCAATTGACCCCCTA

4

H

MTIC331

CCCTCTTCTACCTCCTTTCCA

GGAAGAGAAGATGGGGGTGT

4

H

MTIC332

CCCTGGGTTTTTGATCCAG

GGTCATACGAGCTCCTCCAT

4

H

MTIC338

TCCCCTTAAGCTTCACTCTTTTC

CATTGGTGGACGAGGTCTCT

3

H

MTIC339

CCACACAAAACACGCACTCT

GGTAGGATTGCCACGACTGT

4

H

MTIC343

TCCGATCTTGCGTCCTAACT

CCATTGCGGTGGCTACTCT

6

H

MTIC345

TCCGATCTTGCGTCCTAACT

CCATTGCGGTGGCTACTCT

H

MTIC347

TCGGTGTATTTCCGTGTTTG

GGTTGAAATTGAAAGAAGAATCG

4

H

MTIC354

AAGTGCCAAAGAACAGGGTTT

AACCTACGCTAGGGTTGCAG

2

H

MTIC356

CGGCGATGGAAAATTGATAG

CCAATACAAACTTTGCGTGAGA

8

H

MTIC365

ATCGGCGTCTCAGATTGATT

CGCCATATCCAAATCCAAAT

2

H

MTIC432

TGGAATTTGGGATATAGGAA

GGCCATAAGAACTTCCACTT

7

H

MTIC441

CTTCCTTATCATCGCTTCC

CAGAGATTGAGAATCGAGAAG

H

MTIC446

ATAACTGGCTGAACAAATGC

TCTCCTTCCACCCTCTATG

7

H

MTIC447

TCTTGTTGTATCCTCCGAAC

TCCTGAGTTGTAGAGTGAGTGA

1

H

MTIC451

GGACAAAATTGGAAGAAAAA

AATTACGTTTGTTTGGATGC

2

H

MTIC452

CTAGTGCCAACACAAAAACA

TCACAAAAACTGCATAAAGC

2

H

MTIC470

GGTTCGTGTATTTGTTCGAT

CCCTTCACAGAATGATTGAT

7

H

MTIC471

ATCAGGTGATGATTGGTTTT

CCAACCATCTTTGTTTCCTA

H

MTIC475

GGATTGAAATGCACTCTCTC

TTAATAAACGCCGCTCCT

3

H