Figure 4

Verdejo experimental conditions throughout the 24 h sampling cycle, clustering of Verdejo pericarp DEG and functional enrichment analysis. A. Verdejo berry sampling set up. Graph indicating temperature, irradiation and relative humidity conditions measured in a Valbuena de Duero station near the Verdejo vineyard throughout the time lapse of the experimental sampling (2011/09/12 and 2011/09/13 dates). Verdejo berries were harvested at six time points spread throughout 24 h as specified. B. Clustering and functional enrichment analysis. Significant transcripts identified (5% FDR in Limma and ≥2-fold change) were clustered in a 3x1 SOM analysis. Log₂ expression normalized to the last time point is represented within each cluster; no difference of expression is represented in black, higher expression in magenta and lower expression in green. Number of genes within each cluster appears in white. Time points in the light period are indicated in yellow. A summary of over-represented functional categories (5% FDR) ordered by their significance level is indicated for each cluster profile.