Figure 4From: Co-overexpression of two Heat Shock Factors results in enhanced seed longevity and in synergistic effects on seedling tolerance to severe dehydration and oxidative stressEnhanced resistance to drastic dehydration and oxidative stress conditions in the 35S:A9/A4a seedlings. Leaf-survival and whole seedling survival was evaluated. (A) Tolerance to severe dehydration. Data correspond to 17 independent experiments (n = 162) performed with the four sibling 35S:A9/A4a and 35S:A9 line pairs. (B) Tolerance to drastic oxidative stress conditions (treatments with 300 mM H2O2 for 24 h). Data correspond to 10 independent experiments (n = 100) performed with the same sibling line pairs. The dashed line in (A) and (B) separates the data that are described by the y-axis labels placed respectively to right or left in these panels. Data are mean values ± SE. Asterisks denote statistically significant differences (P ≤ 0.01). (C) Representative results shown for survival of leaves and whole seedlings after the H2O2 treatments. Scale bars, 1 cm.Back to article page